##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049485_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10021620 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.219928314983008 31.0 31.0 34.0 28.0 34.0 2 31.340339086894136 31.0 31.0 34.0 28.0 34.0 3 31.430417736852924 31.0 31.0 34.0 28.0 34.0 4 35.078755231190165 37.0 35.0 37.0 32.0 37.0 5 35.199480622893304 37.0 35.0 37.0 32.0 37.0 6 35.25792965608355 37.0 35.0 37.0 32.0 37.0 7 35.205667646548164 37.0 35.0 37.0 32.0 37.0 8 35.18404319860462 37.0 35.0 37.0 32.0 37.0 9 36.79627645031442 39.0 37.0 39.0 32.0 39.0 10-11 36.66313355525354 39.0 35.0 39.0 32.0 39.0 12-13 36.61023966185108 39.0 35.0 39.0 32.0 39.0 14-15 37.701062951897995 39.0 37.0 41.0 32.0 41.0 16-17 37.70235999768501 39.0 37.0 41.0 32.0 41.0 18-19 37.74930909373934 39.5 37.0 41.0 32.0 41.0 20-21 37.78372773064634 40.0 37.0 41.0 32.0 41.0 22-23 37.653457125694246 39.0 37.0 41.0 32.0 41.0 24-25 37.4661359141536 39.0 36.0 41.0 32.0 41.0 26-27 37.39533209201706 39.0 36.0 41.0 32.0 41.0 28-29 37.319207273873886 39.0 36.0 41.0 32.0 41.0 30-31 37.1583325350592 39.0 36.0 41.0 31.0 41.0 32-33 36.95780193222254 39.0 35.5 41.0 31.0 41.0 34-35 36.79926478952504 39.0 35.0 41.0 30.5 41.0 36-37 36.62757523234767 39.0 35.0 41.0 30.0 41.0 38-39 36.48781344732688 39.0 35.0 40.5 30.0 41.0 40-41 36.360250887581046 39.0 35.0 40.0 30.0 41.0 42-43 36.152389533827865 39.0 35.0 40.0 30.0 41.0 44-45 35.955557734178704 39.0 35.0 40.0 29.0 41.0 46-47 35.67116713665057 38.0 35.0 40.0 28.0 41.0 48-49 35.35760346131663 38.0 34.5 40.0 27.0 41.0 50-51 35.142468982060784 38.0 34.0 40.0 26.5 41.0 52-53 34.97074829219228 38.0 34.0 40.0 26.0 41.0 54-55 34.756167565722905 38.0 34.0 40.0 26.0 41.0 56-57 34.562704482907954 37.0 34.0 40.0 26.0 41.0 58-59 34.315815057844944 37.0 33.5 40.0 26.0 41.0 60-61 34.031772757298725 36.5 33.0 39.5 26.0 41.0 62-63 33.68864360253133 36.0 33.0 39.0 25.5 41.0 64-65 33.37793031465971 35.5 33.0 39.0 25.0 40.5 66-67 33.011946172375325 35.0 32.5 38.5 24.0 40.0 68-69 32.67841207309796 35.0 32.0 37.5 23.5 40.0 70-71 32.356837966316824 35.0 32.0 37.0 23.5 39.5 72-73 32.03184275596161 35.0 32.0 36.5 23.0 39.0 74-75 31.766300857545986 35.0 32.0 36.0 22.5 39.0 76-77 31.514328222383206 35.0 32.0 36.0 22.0 38.0 78-79 31.271403076548502 35.0 31.0 35.0 21.5 37.0 80-81 30.993764531083798 34.5 31.0 35.0 20.0 37.0 82-83 30.829618065741865 34.0 31.0 35.0 20.0 36.0 84-85 30.568645588238226 34.0 31.0 35.0 19.0 36.0 86-87 30.428136718414788 34.0 31.0 35.0 18.5 36.0 88-89 30.28674141506064 34.0 31.0 35.0 18.0 35.0 90-91 30.13596644055552 34.0 31.0 35.0 17.0 35.0 92-93 29.875937772535778 34.0 31.0 35.0 10.5 35.0 94-95 29.71249663228101 34.0 31.0 35.0 4.5 35.0 96-97 29.54027038542671 34.0 31.0 35.0 2.0 35.0 98-99 29.325360919691626 34.0 30.5 35.0 2.0 35.0 100 29.181764724665275 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.159433618950807 1101 2 0.16866402072095 1101 3 0.23153819401353815 1101 4 0.3251819309899773 1101 5 0.257162231527289 1101 6 0.25392714149618456 1101 7 0.2013931279487693 1101 8 0.19930978212858008 1101 9 0.20627481870302944 1101 10-11 0.31525507515813445 1101 12-13 0.3829647026645304 1101 14-15 0.349563507454306 1101 16-17 0.3922606736485079 1101 18-19 0.4277876901280777 1101 20-21 0.3179182432981449 1101 22-23 0.31756226165928325 1101 24-25 0.41466723215490475 1101 26-27 0.2879395314459714 1101 28-29 0.21366820542881015 1101 30-31 0.21541248491610077 1101 32-33 0.2019601279188663 1101 34-35 0.3331896835110939 1101 36-37 0.35586003997956794 1101 38-39 0.4174927440062177 1101 40-41 0.36252732675389154 1101 42-43 0.3701160892500397 1101 44-45 0.3309252265386107 1101 46-47 0.3363303930074153 1101 48-49 0.3792860825911504 1101 50-51 0.4536878512621385 1101 52-53 0.40604555884276294 1101 54-55 0.27567344119337633 1101 56-57 0.3065828709305194 1101 58-59 0.31634189336352847 1101 60-61 0.30171626709338994 1101 62-63 0.3058500555246084 1101 64-65 0.2836883198015414 1101 66-67 0.31724681981695113 1101 68-69 0.2700728924847908 1101 70-71 0.3154369304741991 1101 72-73 0.3303548974749404 1101 74-75 0.30287219887408057 1101 76-77 0.3001530257295073 1101 78-79 0.3164886446682331 1101 80-81 0.3919783180873786 1101 82-83 0.29761873313308485 1101 84-85 0.32210902261610386 1101 86-87 0.34481855806676265 1101 88-89 0.27800075019497683 1101 90-91 0.3359600732054915 1101 92-93 0.3670934308518916 1101 94-95 0.3073505882702534 1101 96-97 0.30865730111333534 1101 98-99 0.22881872942683756 1101 100 0.24680388560280875 1102 1 0.14333295260939494 1102 2 0.20255242518619454 1102 3 0.21897385704473749 1102 4 0.24266474158449114 1102 5 0.18595198381233757 1102 6 0.2586339099420343 1102 7 0.19894577225343824 1102 8 0.23407067339671528 1102 9 0.29710398936846616 1102 10-11 0.31878992146045704 1102 12-13 0.31213308624373326 1102 14-15 0.42578598091093767 1102 16-17 0.382935900238202 1102 18-19 0.4154634863823006 1102 20-21 0.32255553507438606 1102 22-23 0.261207632429894 1102 24-25 0.484877145509941 1102 26-27 0.2799325039277534 1102 28-29 0.33024010321411623 1102 30-31 0.3108531902207403 1102 32-33 0.3650280107086843 1102 34-35 0.4199592562174388 1102 36-37 0.4189235618529423 1102 38-39 0.3421646998947665 1102 40-41 0.35816914190831284 1102 42-43 0.416337823516848 1102 44-45 0.31627268521282303 1102 46-47 0.392895528544031 1102 48-49 0.4518124344738723 1102 50-51 0.4113363165313402 1102 52-53 0.34639608547206535 1102 54-55 0.3533794195145532 1102 56-57 0.3108464427895683 1102 58-59 0.38862450436850793 1102 60-61 0.3033691454831171 1102 62-63 0.3258205627059496 1102 64-65 0.3141195425616772 1102 66-67 0.3499798366479112 1102 68-69 0.35215179668043817 1102 70-71 0.3689553429781043 1102 72-73 0.3495139099813471 1102 74-75 0.31316954218887716 1102 76-77 0.3021649933755626 1102 78-79 0.2998168031209545 1102 80-81 0.3533134960566393 1102 82-83 0.2517418092598156 1102 84-85 0.3806295966777071 1102 86-87 0.27963796370075045 1102 88-89 0.24151476536097505 1102 90-91 0.27483626241402703 1102 92-93 0.38258494159199685 1102 94-95 0.29024499350001776 1102 96-97 0.28835554500911087 1102 98-99 0.2661641500451637 1102 100 0.1948252656544831 1103 1 0.08327074435793591 1103 2 0.1450153399975207 1103 3 0.1287355283715712 1103 4 0.22952296284469043 1103 5 0.21862962159146093 1103 6 0.15000827659044091 1103 7 0.1609240200015165 1103 8 0.18506454164632657 1103 9 0.19287472272898754 1103 10-11 0.2747691373311696 1103 12-13 0.2829837658866339 1103 14-15 0.31807929278493674 1103 16-17 0.26976485564806296 1103 18-19 0.2524543086022888 1103 20-21 0.28913420759042907 1103 22-23 0.24876965291493747 1103 24-25 0.37348596697990644 1103 26-27 0.20169053422919347 1103 28-29 0.2209152487971835 1103 30-31 0.21702114381079696 1103 32-33 0.22834675593608011 1103 34-35 0.22465954260538012 1103 36-37 0.18098946279562256 1103 38-39 0.19075432361583466 1103 40-41 0.2376036754164801 1103 42-43 0.29190722179532 1103 44-45 0.14590204003067697 1103 46-47 0.1969506106496084 1103 48-49 0.24074995954805445 1103 50-51 0.276583147451646 1103 52-53 0.3250515440893338 1103 54-55 0.33259708436916213 1103 56-57 0.27924409507086523 1103 58-59 0.26001272318028157 1103 60-61 0.2703104137320125 1103 62-63 0.304899205491445 1103 64-65 0.22250498201817948 1103 66-67 0.26703233011361505 1103 68-69 0.22640476100092144 1103 70-71 0.23669994486402857 1103 72-73 0.26464687602531 1103 74-75 0.21435449223050185 1103 76-77 0.3099407979620068 1103 78-79 0.2483427821758326 1103 80-81 0.287608946797274 1103 82-83 0.3029969103159509 1103 84-85 0.2193446527581706 1103 86-87 0.20253860225501796 1103 88-89 0.20065126150962342 1103 90-91 0.21014048929397333 1103 92-93 0.31261560989893766 1103 94-95 0.17865772401155766 1103 96-97 0.2188572825581332 1103 98-99 0.1935829714748465 1103 100 0.16039169060395153 1104 1 0.015771023025095587 1104 2 0.004442890074656702 1104 3 0.06936095286145516 1104 4 0.1573055686741185 1104 5 0.10226459847790181 1104 6 0.12575830233478058 1104 7 0.09260573701016739 1104 8 0.18088848617876607 1104 9 0.19688916418214575 1104 10-11 0.2406556939356932 1104 12-13 0.18492730799486878 1104 14-15 0.15185069638746995 1104 16-17 0.16178251706944025 1104 18-19 0.16633380541781406 1104 20-21 0.09380899839506895 1104 22-23 0.03270769364561943 1104 24-25 0.21779974887636655 1104 26-27 0.08882743107822932 1104 28-29 0.07513754873553324 1104 30-31 0.09527671294669915 1104 32-33 0.1388438215628227 1104 34-35 0.1580826495977803 1104 36-37 0.1456087755799942 1104 38-39 0.1288048353975597 1104 40-41 0.12331030026861356 1104 42-43 0.17931652989543778 1104 44-45 0.12229572074701878 1104 46-47 0.12156238302046063 1104 48-49 0.20264213855337232 1104 50-51 0.16937334783226987 1104 52-53 0.14056362392086896 1104 54-55 0.109445774885927 1104 56-57 0.1444970474707219 1104 58-59 0.11754682109739889 1104 60-61 0.10553894479654247 1104 62-63 0.1955844308893404 1104 64-65 0.1546134537273005 1104 66-67 0.11418501001323733 1104 68-69 0.09528784091340015 1104 70-71 0.1840672078972645 1104 72-73 0.1510465652745907 1104 74-75 0.0952224595914899 1104 76-77 0.06704505816853157 1104 78-79 0.0597197151013944 1104 80-81 0.09951061340190037 1104 82-83 0.04145621852525494 1104 84-85 0.04872931340226572 1104 86-87 -0.04127686514818407 1104 88-89 -0.06352519736874385 1104 90-91 -0.08577641904804523 1104 92-93 0.05100579893721502 1104 94-95 -0.003234536347818562 1104 96-97 -0.01171567578220234 1104 98-99 -0.049264952379786564 1104 100 -0.052651236341844765 1105 1 -0.07182866288922085 1105 2 -0.03784763356856047 1105 3 -0.033517736739085535 1105 4 0.08530163350688014 1105 5 0.050396756670188836 1105 6 0.05491737456217294 1105 7 0.05734122190402502 1105 8 0.09915320947153106 1105 9 0.07179731495720176 1105 10-11 0.1102646249953807 1105 12-13 0.06665071647179133 1105 14-15 0.07372018882479381 1105 16-17 0.0040054054756808455 1105 18-19 0.06028370442719222 1105 20-21 0.0716705899073915 1105 22-23 0.029649770914502938 1105 24-25 0.2377353707222838 1105 26-27 0.10627719131001356 1105 28-29 0.0013485385188616306 1105 30-31 0.011090861714450284 1105 32-33 -0.05487157080671068 1105 34-35 -0.005835425441659936 1105 36-37 0.07072195630917832 1105 38-39 0.10669237488669125 1105 40-41 0.0943778495191765 1105 42-43 0.12233262473986173 1105 44-45 0.02215172207184679 1105 46-47 -0.03624655173348401 1105 48-49 0.01564358331764737 1105 50-51 -0.010477372035587962 1105 52-53 0.027927543584816306 1105 54-55 -0.07608628329508349 1105 56-57 0.027587385289002953 1105 58-59 -0.005717922574064005 1105 60-61 -0.0038453632472510435 1105 62-63 0.008151636171767507 1105 64-65 0.004225437902960039 1105 66-67 0.05310081392992316 1105 68-69 -0.03654499397854494 1105 70-71 -0.03372514255460857 1105 72-73 -0.03146318566288997 1105 74-75 -0.01120640853320154 1105 76-77 -0.013725003736016816 1105 78-79 -0.06719107245235989 1105 80-81 -0.009604317933980866 1105 82-83 -0.09966896564442607 1105 84-85 -0.033101954624164875 1105 86-87 -0.07627166165211818 1105 88-89 -0.10206965055818529 1105 90-91 -0.12296434817879387 1105 92-93 -0.12372516647409171 1105 94-95 -0.16097806807457715 1105 96-97 -0.18363675376912525 1105 98-99 -0.19834590860088142 1105 100 -0.2541014178413725 1106 1 -0.19243661386010658 1106 2 -0.05897266667347267 1106 3 -0.03027927323855195 1106 4 0.1102701242882489 1106 5 0.009318810757655172 1106 6 -0.05089821829217556 1106 7 0.009181484545578655 1106 8 -9.744405418601332E-4 1106 9 -0.07850009443573214 1106 10-11 0.05959533162329933 1106 12-13 0.05404606274601065 1106 14-15 0.04047914752298709 1106 16-17 -0.014495510126010913 1106 18-19 0.06187847068537877 1106 20-21 0.004546152813894366 1106 22-23 -0.035293779380104695 1106 24-25 0.16992583945865647 1106 26-27 -0.025111955840642963 1106 28-29 0.007800753986153097 1106 30-31 -0.12734266064958888 1106 32-33 -0.061436922958463924 1106 34-35 -0.20866393694367957 1106 36-37 -0.15326916557575743 1106 38-39 -0.005129867571199043 1106 40-41 0.012769639810841227 1106 42-43 0.11230192285115237 1106 44-45 0.04595146109049608 1106 46-47 -0.041685135783708915 1106 48-49 0.015541961384805347 1106 50-51 -0.008713161331236563 1106 52-53 -0.015323069097043174 1106 54-55 -0.0815667168019587 1106 56-57 -0.10854991711032369 1106 58-59 0.0048126398716306085 1106 60-61 -0.037819212915295 1106 62-63 0.018925614382723666 1106 64-65 0.035177177485330446 1106 66-67 0.046824916419225815 1106 68-69 0.06872232402223943 1106 70-71 0.024862866530618533 1106 72-73 -5.99893598625556E-4 1106 74-75 0.05747941366985643 1106 76-77 0.09692583418225809 1106 78-79 0.018232371712556983 1106 80-81 0.024172644691237366 1106 82-83 -0.042872014886651044 1106 84-85 -0.10038709497319687 1106 86-87 -0.14081127186533848 1106 88-89 -0.15312471047689868 1106 90-91 -0.10879475963272256 1106 92-93 -0.015073909461801094 1106 94-95 -0.09034255551232206 1106 96-97 -0.20462792676810437 1106 98-99 -0.2813191912188451 1106 100 -0.28901288769798583 1107 1 -0.18580546557448585 1107 2 -0.1365485949567926 1107 3 -0.08673129181756423 1107 4 0.03335663602645411 1107 5 -0.021011298444264526 1107 6 -0.06651435561524721 1107 7 -0.05487466249400086 1107 8 0.03140667308321099 1107 9 -0.030178384883313925 1107 10-11 0.044673534290843975 1107 12-13 -0.002470340337239918 1107 14-15 0.03884153575952354 1107 16-17 -0.019727374002265208 1107 18-19 -0.054621781215907106 1107 20-21 -0.017962035956180955 1107 22-23 -0.03648470711424068 1107 24-25 0.09511692009571249 1107 26-27 -0.045870251712997856 1107 28-29 -0.16845252760759166 1107 30-31 -0.13294478599124915 1107 32-33 -0.181091144274788 1107 34-35 -0.16777857872898494 1107 36-37 -0.1949075937757314 1107 38-39 -0.13971097616767736 1107 40-41 -0.1886419484912878 1107 42-43 -0.14952906264860388 1107 44-45 -0.22294811035635576 1107 46-47 -0.18609013903603255 1107 48-49 -0.0680509629851116 1107 50-51 -0.07850248541802074 1107 52-53 -0.12811959944729523 1107 54-55 -0.10409209581457901 1107 56-57 -0.25185401135399843 1107 58-59 -0.22526540045103616 1107 60-61 -0.14208085896899547 1107 62-63 -0.16939679558205256 1107 64-65 -0.12450534153964554 1107 66-67 -0.085895811851735 1107 68-69 -0.0493538445866335 1107 70-71 -0.09334606747359686 1107 72-73 -0.09720512031431738 1107 74-75 -0.0826443679011355 1107 76-77 -0.11231116194381485 1107 78-79 -0.14021317444451853 1107 80-81 -0.1484793966694049 1107 82-83 -0.21537462738564983 1107 84-85 -0.21400219115524877 1107 86-87 -0.2547800482858378 1107 88-89 -0.23555110329863282 1107 90-91 -0.17761189926562793 1107 92-93 -0.20047282017565138 1107 94-95 -0.32339534796964386 1107 96-97 -0.3209238028222501 1107 98-99 -0.2372008916059798 1107 100 -0.25878357724177903 1108 1 -0.13897857865359597 1108 2 -0.09975635385547577 1108 3 -0.08442420770087722 1108 4 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2215 38-39 -0.3055982375000781 2215 40-41 -0.2865533773347977 2215 42-43 -0.10952167490680864 2215 44-45 -0.31742707091019895 2215 46-47 -0.13083803345524814 2215 48-49 -0.02411672100764406 2215 50-51 0.00922787792539026 2215 52-53 -0.12161650208021513 2215 54-55 -0.11793520295243809 2215 56-57 -0.10840689306224505 2215 58-59 -0.181696241532336 2215 60-61 -0.1946047036936207 2215 62-63 -0.23459016728646276 2215 64-65 -0.1952643468769324 2215 66-67 -0.06596756510393931 2215 68-69 -0.1635431247403787 2215 70-71 -0.10907674912537146 2215 72-73 -0.09974932848331264 2215 74-75 -0.07935161445489669 2215 76-77 0.04529712659358154 2215 78-79 -0.00874580290424376 2215 80-81 0.08312549734874963 2215 82-83 0.10525610360107862 2215 84-85 0.17679600997519884 2215 86-87 0.1296949505028664 2215 88-89 0.16037306762720505 2215 90-91 0.0957911440130772 2215 92-93 0.11725076368675147 2215 94-95 0.12496430451027152 2215 96-97 0.15190844451873886 2215 98-99 0.2183051130071192 2215 100 0.3118168752067696 2216 1 -0.1358024563600715 2216 2 -0.18738774290104487 2216 3 -0.18553346907211932 2216 4 -0.13500041600740786 2216 5 -0.16127900663229155 2216 6 -0.2244293395743071 2216 7 -0.17335168312995108 2216 8 -0.1322318669522886 2216 9 -2.326690795353052 2216 10-11 -0.8239367538917861 2216 12-13 -0.546269339553831 2216 14-15 -0.516404925280824 2216 16-17 -0.6763783631716933 2216 18-19 -0.6472290337808957 2216 20-21 -0.8178097585828752 2216 22-23 -0.3141066861807431 2216 24-25 -0.10192090419989341 2216 26-27 -0.25167333317146756 2216 28-29 -0.2711901135920698 2216 30-31 -0.2336860702585284 2216 32-33 -0.19183634837305874 2216 34-35 -0.20988487438997083 2216 36-37 -0.4700346547665575 2216 38-39 -0.4457202697898026 2216 40-41 -0.32069222131555364 2216 42-43 -0.272464162048621 2216 44-45 -0.3557947269106734 2216 46-47 -0.2978480476629386 2216 48-49 -0.21386351680273208 2216 50-51 -0.3602921221579862 2216 52-53 -0.3442125787578547 2216 54-55 -0.3315308767868075 2216 56-57 -0.2893277363480564 2216 58-59 -0.2729588376038734 2216 60-61 -0.2611938900354218 2216 62-63 -0.2218026530984858 2216 64-65 -0.21632805943495015 2216 66-67 -0.14041708411846798 2216 68-69 -0.23168143627233917 2216 70-71 -0.20212268423681223 2216 72-73 -0.23938857709142525 2216 74-75 -0.2339493187614572 2216 76-77 -0.3063621119108433 2216 78-79 -0.24768476836838005 2216 80-81 -0.20858051437130243 2216 82-83 -0.23503243709209443 2216 84-85 -0.20030512480312979 2216 86-87 -0.25622451005614266 2216 88-89 -0.25172183755234556 2216 90-91 -0.24409879652116473 2216 92-93 -0.26962515015909005 2216 94-95 -0.31716583040143576 2216 96-97 -0.3566717445776675 2216 98-99 -0.2984110385526506 2216 100 -0.3498507974643026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 373.0 3 69.0 4 175.0 5 580.0 6 1975.0 7 5184.0 8 10656.0 9 14690.0 10 18832.0 11 25966.0 12 33632.0 13 40684.0 14 41858.0 15 48242.0 16 60976.0 17 75081.0 18 68531.0 19 46808.0 20 41345.0 21 42999.0 22 47485.0 23 54770.0 24 66814.0 25 83195.0 26 105707.0 27 133796.0 28 172573.0 29 221037.0 30 280307.0 31 350028.0 32 438518.0 33 555079.0 34 729950.0 35 997530.0 36 1459025.0 37 2060507.0 38 1447423.0 39 238910.0 40 310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.560215186743754 17.38951721227442 11.778036566694226 24.272231034287604 2 19.950526960710942 18.889341244229975 35.41502272087747 25.74510907418162 3 19.15485683261054 23.63192174122072 29.076594261489845 28.136627164678895 4 14.096454874207451 17.200833518017397 34.61751040425703 34.08520120351811 5 13.420956047809376 33.10423460114647 31.08933593233132 22.385473418712827 6 30.820003798055605 34.895099919498385 17.939396165041583 16.345500117404423 7 27.54220375548065 29.822553639032414 21.484759948990284 21.150482656496656 8 26.934028630101718 30.0112257299718 21.27848591345511 21.77625972647137 9 24.915921777117873 16.054340515804828 22.077747909020697 36.9519897980566 10-11 24.938258485155096 23.81478742957725 26.948706895691515 24.298247189576134 12-13 26.913196360787612 21.9640010261815 26.915077294397243 24.207725318633646 14-15 24.58540994858464 23.709618104025434 25.52102248175232 26.183949465637607 16-17 22.966907030453672 26.07845641645583 25.174355630305094 25.780280922785398 18-19 22.491822679367207 23.85963047890461 28.877616592926092 24.770930248802088 20-21 24.746912175875757 24.173676511382393 27.750363713651087 23.329047599090767 22-23 23.72723172501053 25.042752568945936 27.7562908990762 23.473724806967336 24-25 23.27745913335369 24.290254469836214 28.72538072686851 23.706905669941587 26-27 23.476681756263194 23.379227107250355 28.90819809981146 24.235893036674987 28-29 23.666697184059544 23.59834986552806 28.671348383979318 24.06360456643308 30-31 24.28734112765841 25.3761285302782 27.50873549976888 22.827794842294512 32-33 23.084945348157284 24.22447668141478 28.813590018380264 23.876987952047674 34-35 22.75581692381072 22.744551280132356 29.20883050844075 25.290801287616173 36-37 24.571820723595586 24.4039935659105 28.05134299644169 22.97284271405222 38-39 24.28169297977772 24.229036822389993 28.003566289681707 23.48570390815058 40-41 23.368877654953874 23.628321749793034 28.64224190511324 24.360558690139854 42-43 22.77144313993147 24.293252987042017 28.557842943556032 24.377460929470484 44-45 23.745252763525258 24.49617427122561 27.950545919721563 23.80802704552757 46-47 23.602681003670064 25.5270455275694 26.82115266793193 24.049120800828607 48-49 23.326011161671815 24.402678729390676 28.137215780646972 24.134094328290534 50-51 23.425963922490556 23.69397953972999 27.83851010078962 25.041546436989837 52-53 23.208493237620264 24.212288033272067 27.513381070126385 25.065837658981284 54-55 23.60844354505559 25.724753083832752 26.97341348005612 23.69338989105554 56-57 22.392956428202226 24.99543486981147 26.994293337803672 25.617315364182637 58-59 22.315663535436386 23.895148688535386 28.05749469646624 25.731693079561985 60-61 23.717812090260857 25.241163604287532 26.82487462106925 24.216149684382366 62-63 23.285591551066595 25.24915133481413 26.82469999860302 24.640557115516255 64-65 23.081393028272874 24.155979771733513 27.65992923299826 25.102697966995358 66-67 22.60809611231468 24.78896183936607 27.075279276610548 25.527662771708698 68-69 24.033903700200167 24.885233125981628 26.657820791448888 24.423042382369317 70-71 24.02009854694151 24.10492515182176 27.054448282812558 24.820528018424167 72-73 22.925495079637823 24.706339893151007 27.705415890843994 24.662749136367175 74-75 22.774252067031078 24.16624757274772 27.505273598480084 25.554226761741116 76-77 23.26382361334794 24.823865802135785 26.37752678708632 25.534783797429956 78-79 23.467988076775033 25.47059893275711 26.747483627268753 24.313929363199104 80-81 23.014856879426677 24.52395421099583 27.08162951698428 25.379559392593215 82-83 22.779712096361298 24.717303604797507 27.118547835144767 25.384436463696424 84-85 23.010157394284384 25.10236498297106 26.526796004097342 25.360681618647213 86-87 23.606191119443103 25.046543158598478 26.360882542604795 24.986383179353624 88-89 22.801786657960154 24.801499079589558 26.82577340820083 25.570940854249454 90-91 22.1096682684879 24.681958648674687 26.893164458996548 26.315208623840864 92-93 23.02395871324133 25.22720639338071 26.11655406838119 25.632280824996766 94-95 23.059368328326737 24.571160664781107 26.63005546661169 25.739415540280458 96-97 22.195815415606855 24.7940738901753 26.99364418903876 26.016466505179082 98-99 22.36480347728385 24.777059386136866 27.03331748425778 25.82481965232151 100 22.734224980352447 24.948211883047865 26.482437342365557 25.835125794234138 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4944.0 1 4333.0 2 22335.0 3 32971.0 4 16041.5 5 5961.0 6 5920.0 7 12721.0 8 20121.5 9 21280.0 10 15988.5 11 9334.0 12 7380.0 13 7628.0 14 8405.5 15 9405.0 16 10229.0 17 10992.0 18 12044.5 19 13113.5 20 14253.5 21 15617.5 22 17875.0 23 21514.5 24 25329.0 25 28950.5 26 34340.0 27 42857.0 28 54984.5 29 65869.5 30 72085.5 31 80419.5 32 91423.0 33 101873.5 34 114550.0 35 129319.0 36 142117.0 37 156002.0 38 171518.0 39 184031.5 40 199332.5 41 212774.0 42 223760.0 43 238769.0 44 252446.5 45 266457.0 46 285127.0 47 305895.0 48 326318.5 49 353449.5 50 381551.0 51 398845.5 52 405673.5 53 418574.5 54 437701.0 55 440388.0 56 425341.5 57 398746.5 58 367449.5 59 340500.0 60 307317.0 61 266152.5 62 225967.0 63 183023.5 64 144461.5 65 112540.5 66 82406.0 67 58237.5 68 42250.0 69 30438.0 70 21443.5 71 15996.0 72 11994.5 73 8893.0 74 6962.5 75 4583.0 76 2427.0 77 1423.0 78 900.5 79 603.0 80 366.5 81 211.0 82 119.0 83 49.5 84 30.0 85 30.0 86 26.5 87 21.0 88 15.0 89 12.0 90 7.5 91 3.0 92 2.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03152184976081711 2 0.0 3 9.87864237518485E-4 4 9.97842664160086E-6 5 0.0032130533785954766 6 0.007014833929045405 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.97842664160086E-6 14-15 3.4924493245603005E-5 16-17 4.989213320800429E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.542341457768305E-4 28-29 1.2473033302001074E-4 30-31 1.2473033302001074E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.98921332080043E-6 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.993527992480258E-5 50-51 2.4946066604002144E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.023369475194629213 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.4946066604002144E-5 80-81 0.0 82-83 2.993527992480258E-5 84-85 4.490291988720387E-5 86-87 7.483819981200644E-5 88-89 3.991370656640344E-5 90-91 6.485977317040558E-5 92-93 4.989213320800429E-5 94-95 5.488134652880473E-5 96-97 3.4924493245603005E-5 98-99 4.989213320800429E-5 100 1.5965482626561375E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.002162E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.41029760274612 #Duplication Level Percentage of deduplicated Percentage of total 1 63.79259879865948 25.778779023064146 2 19.236836679791452 15.547325903315906 3 7.649035775681715 9.272994362080503 4 3.2807773276545174 5.303087527154447 5 1.5940540746479375 3.220809977569662 6 0.8995720314171028 2.1811184102803205 7 0.6106260231272413 1.727290552298721 8 0.42689727943553457 1.3800836886234098 9 0.31653223804180813 1.1512045746119468 >10 1.894032664261804 14.51419904066807 >50 0.1767390566631013 4.960870731576509 >100 0.11052746628386759 8.476587456791556 >500 0.008147083499696253 2.1825499495819867 >1k 0.0033753160451044434 2.2097796934426284 >5k 7.445543687715396E-5 0.24170423110864434 >10k+ 1.7372935271335924E-4 1.851614877831541 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49270 0.49163708063167433 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35045 0.34969396165490213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33608 0.3353549625709217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23159 0.23109038259283432 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21780 0.21733013225406672 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 11534 0.1150911728842243 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10226 0.10203939083701039 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.97842664160086E-6 0.0 0.0 1.995685328320172E-5 0.0 2 9.97842664160086E-6 0.0 0.0 1.995685328320172E-5 0.0 3 9.97842664160086E-6 0.0 0.0 1.995685328320172E-5 0.0 4 9.97842664160086E-6 0.0 0.0 1.995685328320172E-5 0.0 5 9.97842664160086E-6 0.0 0.0 2.9935279924802576E-5 0.0 6 9.97842664160086E-6 0.0 0.0 2.9935279924802576E-5 0.0 7 9.97842664160086E-6 0.0 0.0 2.9935279924802576E-5 0.0 8 9.97842664160086E-6 0.0 0.0 2.9935279924802576E-5 0.0 9 9.97842664160086E-6 0.0 0.0 2.9935279924802576E-5 0.0 10-11 9.97842664160086E-6 0.0 0.0 4.490291988720387E-5 0.0 12-13 9.97842664160086E-6 0.0 0.0 1.0976269305760945E-4 0.0 14-15 9.97842664160086E-6 0.0 0.0 1.2971954634081117E-4 0.0 16-17 9.97842664160086E-6 0.0 0.0 1.696332529072146E-4 0.0 18-19 9.97842664160086E-6 1.496763996240129E-5 0.0 2.0954695947361803E-4 0.0 20-21 9.97842664160086E-6 1.995685328320172E-5 0.0 3.9414785234323394E-4 0.0 22-23 9.97842664160086E-6 1.995685328320172E-5 0.0 0.0013321199566537147 0.0 24-25 9.97842664160086E-6 3.991370656640344E-5 0.0 0.0035473306710891053 0.0 26-27 9.97842664160086E-6 5.987055984960515E-5 0.0 0.007608550314220655 0.0 28-29 9.97842664160086E-6 5.987055984960515E-5 0.0 0.01571103274720055 0.0 30-31 9.97842664160086E-6 5.987055984960515E-5 0.0 0.03820240639736889 0.0 32-33 9.97842664160086E-6 5.987055984960515E-5 0.0 0.0917815682494447 0.0 34-35 9.97842664160086E-6 6.984898649120601E-5 0.0 0.1660210624629551 0.0 36-37 9.97842664160086E-6 8.980583977440774E-5 0.0 0.26552593293299886 0.0 38-39 9.97842664160086E-6 9.978426641600859E-5 0.0 0.4223568644590395 0.0 40-41 9.97842664160086E-6 1.0477347973680902E-4 0.0 0.65504379531453 0.0 42-43 9.97842664160086E-6 1.0976269305760945E-4 0.0 0.9011517100029736 0.0 44-45 9.97842664160086E-6 1.0976269305760945E-4 0.0 1.1449695757771696 0.0 46-47 9.97842664160086E-6 1.0976269305760945E-4 0.0 1.4183934333969956 4.98921332080043E-6 48-49 9.97842664160086E-6 1.0976269305760945E-4 0.0 1.7358920014927726 9.97842664160086E-6 50-51 9.97842664160086E-6 1.2971954634081117E-4 0.0 2.0897369886305808 9.97842664160086E-6 52-53 9.97842664160086E-6 1.2971954634081117E-4 0.0 2.44072315653557 9.97842664160086E-6 54-55 9.97842664160086E-6 1.2971954634081117E-4 0.0 2.8142356225839738 9.97842664160086E-6 56-57 9.97842664160086E-6 1.2971954634081117E-4 0.0 3.217234339358307 9.97842664160086E-6 58-59 9.97842664160086E-6 1.4967639962401287E-4 0.0 3.6451791227366432 9.97842664160086E-6 60-61 9.97842664160086E-6 1.696332529072146E-4 0.0 4.0852526836978456 9.97842664160086E-6 62-63 9.97842664160086E-6 1.696332529072146E-4 9.97842664160086E-6 4.52633905496317 9.97842664160086E-6 64-65 1.995685328320172E-5 1.846008928696159E-4 1.995685328320172E-5 5.021733013225407 9.97842664160086E-6 66-67 2.9935279924802576E-5 2.0954695947361803E-4 1.995685328320172E-5 5.579971102476446 9.97842664160086E-6 68-69 2.9935279924802576E-5 2.0954695947361803E-4 1.995685328320172E-5 6.114325827560814 9.97842664160086E-6 70-71 2.9935279924802576E-5 2.0954695947361803E-4 1.995685328320172E-5 6.669286003660087 9.97842664160086E-6 72-73 2.9935279924802576E-5 2.195253861152189E-4 1.995685328320172E-5 7.31923581217408 9.97842664160086E-6 74-75 3.991370656640344E-5 2.195253861152189E-4 1.995685328320172E-5 7.930199907799338 9.97842664160086E-6 76-77 3.991370656640344E-5 2.394822393984206E-4 1.995685328320172E-5 8.527892695991266 9.97842664160086E-6 78-79 3.991370656640344E-5 2.394822393984206E-4 1.995685328320172E-5 9.155525753321319 9.97842664160086E-6 80-81 3.991370656640344E-5 2.394822393984206E-4 1.995685328320172E-5 9.81468066041219 9.97842664160086E-6 82-83 3.991370656640344E-5 2.394822393984206E-4 1.995685328320172E-5 10.513834090695916 1.995685328320172E-5 84-85 3.991370656640344E-5 2.494606660400215E-4 1.995685328320172E-5 11.178307499186758 1.995685328320172E-5 86-87 3.991370656640344E-5 2.5943909268162234E-4 1.995685328320172E-5 11.858347253238499 1.995685328320172E-5 88 3.991370656640344E-5 2.5943909268162234E-4 1.995685328320172E-5 12.412264683753724 1.995685328320172E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 2045 0.0 67.57944 2 ATAACGC 2620 0.0 54.542225 3 GGTATCA 20700 0.0 52.021626 1 GTATAAC 2985 0.0 51.513638 1 TAACGCA 3000 0.0 48.886322 4 GTATCAA 34470 0.0 37.4336 1 TATCACG 1860 0.0 33.359653 2 GTCACGC 2920 0.0 32.995083 8 GCTACGC 2840 0.0 31.117168 2 TACCTGG 7100 0.0 30.05786 2 TGTCACG 3305 0.0 29.435884 7 ATCAACG 43890 0.0 29.06731 3 TCAACGC 44255 0.0 28.859005 4 TCTGTCG 4410 0.0 28.561068 8 TATCAAC 45080 0.0 28.341713 2 CAACGCA 45755 0.0 27.78963 5 GTATACG 410 0.0 27.526196 1 GTGGTAT 9605 0.0 27.514256 1 CGGATAC 2205 0.0 26.860619 4 AACGCAG 48695 0.0 26.44756 6 >>END_MODULE