FastQCFastQC Report
Wed 25 May 2016
SRR2049484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8261205
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8400.035.810472
GGTATCA79000.035.11581
GTATCAA120000.032.9919851
TACCTGG75350.031.3757442
GTACCTG81050.030.0506821
TCAACGC127400.029.5137624
ATCAACG128250.029.2085653
ATAACGC11900.027.6477873
CAACGCA137600.027.4637645
TAACGCA12400.027.290714
AACGCAG146950.025.9077666
TATCAAC148550.025.754972
GTACATG255550.022.9991471
TATCACG12100.022.9179572
ACCTGGG103650.022.2649383
ACGCAGA171550.022.1381057
TACATGG258850.021.9345172
GTATAAC23050.021.418841
CGCAGAG179250.021.0813458
ACATGGG259700.020.9216143
Produced by FastQC (version 0.11.5)