##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049484_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8261205
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.620474373895817	31.0	31.0	34.0	27.0	34.0
2	30.73225274036899	31.0	31.0	34.0	27.0	34.0
3	30.814176261211287	31.0	31.0	34.0	27.0	34.0
4	34.395355520169275	37.0	35.0	37.0	31.0	37.0
5	34.40094356694937	37.0	35.0	37.0	31.0	37.0
6	34.43230243045657	37.0	35.0	37.0	31.0	37.0
7	34.303279001065825	37.0	35.0	37.0	30.0	37.0
8	34.29516856197129	37.0	35.0	37.0	30.0	37.0
9	35.84434837290686	39.0	37.0	39.0	30.0	39.0
10-11	35.79277417761695	39.0	37.0	39.0	30.0	39.0
12-13	35.74747715375662	39.0	37.0	39.0	30.0	39.0
14-15	36.944720836730234	40.0	37.0	41.0	31.0	41.0
16-17	36.92065158775263	40.0	37.0	41.0	30.5	41.0
18-19	36.919193265389254	40.0	37.0	41.0	30.0	41.0
20-21	36.94235992206948	40.0	37.0	41.0	30.0	41.0
22-23	36.88197557136035	40.0	37.0	41.0	30.0	41.0
24-25	36.85353056848244	40.0	37.0	41.0	30.0	41.0
26-27	36.817029174315365	40.0	37.0	41.0	30.0	41.0
28-29	36.765079731104606	40.0	37.0	41.0	30.0	41.0
30-31	36.69738772975613	40.0	37.0	41.0	30.0	41.0
32-33	36.64230738736056	40.0	37.0	41.0	30.0	41.0
34-35	36.56177403901731	40.0	37.0	41.0	30.0	41.0
36-37	36.46620662482047	40.0	36.5	41.0	30.0	41.0
38-39	36.36382585833422	40.0	36.0	41.0	30.0	41.0
40-41	36.237068260623	40.0	36.0	41.0	29.0	41.0
42-43	36.116941233149404	40.0	36.0	41.0	29.0	41.0
44-45	35.9596957102505	40.0	35.5	41.0	28.0	41.0
46-47	35.81820957112188	39.5	35.0	41.0	28.0	41.0
48-49	35.68529221826598	39.0	35.0	41.0	28.0	41.0
50-51	35.533330307140425	39.0	35.0	41.0	27.0	41.0
52-53	35.37701231236848	39.0	35.0	41.0	27.0	41.0
54-55	35.2001689826121	39.0	35.0	41.0	27.0	41.0
56-57	35.008151474270406	39.0	34.5	40.5	26.0	41.0
58-59	34.77893697105931	38.0	34.0	40.0	26.0	41.0
60-61	34.532246506411596	38.0	34.0	40.0	26.0	41.0
62-63	34.24967114361645	37.0	34.0	40.0	25.5	41.0
64-65	33.975042079212415	37.0	34.0	40.0	25.0	41.0
66-67	33.69756464099366	36.5	33.5	39.5	24.5	41.0
68-69	33.38198174479389	36.0	33.0	39.0	23.5	41.0
70-71	33.056306555762745	35.5	33.0	39.0	23.0	41.0
72-73	32.70549647418264	35.0	33.0	38.5	22.5	40.0
74-75	32.386409791307685	35.0	33.0	37.0	22.0	40.0
76-77	32.06957961943809	35.0	32.0	37.0	21.5	39.0
78-79	31.750717056410053	35.0	32.0	36.5	20.0	39.0
80-81	31.44064758107322	35.0	32.0	36.0	19.5	38.5
82-83	31.169351626064234	35.0	32.0	36.0	18.5	37.0
84-85	30.91321453710445	35.0	32.0	35.0	18.0	37.0
86-87	30.657607455570947	35.0	32.0	35.0	14.5	36.5
88-89	30.442733717417738	35.0	31.5	35.0	9.0	36.0
90-91	30.250239644216553	35.0	31.0	35.0	4.5	36.0
92-93	30.039852963338884	35.0	31.0	35.0	2.0	36.0
94-95	29.836810731606345	34.5	31.0	35.0	2.0	35.0
96-97	29.614289864493134	34.0	31.0	35.0	2.0	35.0
98-99	29.411969863960522	34.0	31.0	35.0	2.0	35.0
100	29.269547602317097	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.38913931689356573
1101	2	0.38468791358480203
1101	3	0.3974909748894717
1101	4	0.41894190516823215
1101	5	0.42371650956668816
1101	6	0.48775680665705323
1101	7	0.5304496483098049
1101	8	0.47430217702446953
1101	9	0.6412930008937323
1101	10-11	0.619657703145343
1101	12-13	0.6297296626076445
1101	14-15	0.7332147059761098
1101	16-17	0.6988286738356351
1101	18-19	0.735020508609793
1101	20-21	0.7415270158719238
1101	22-23	0.7148343774687405
1101	24-25	0.7659047798874781
1101	26-27	0.749724658014344
1101	28-29	0.7474670686287652
1101	30-31	0.7397490519958012
1101	32-33	0.7336916960450708
1101	34-35	0.6992115718813423
1101	36-37	0.6944907261731501
1101	38-39	0.7541814630671624
1101	40-41	0.7773357513840864
1101	42-43	0.7206472345815698
1101	44-45	0.6808583106191932
1101	46-47	0.6600093530249538
1101	48-49	0.6403946618736001
1101	50-51	0.6468383356811742
1101	52-53	0.6689790579890555
1101	54-55	0.6849915300518035
1101	56-57	0.6877175044362573
1101	58-59	0.6761413604675042
1101	60-61	0.6811569893474214
1101	62-63	0.7208680336007305
1101	64-65	0.7183767055025712
1101	66-67	0.7011499169200164
1101	68-69	0.6732473271376804
1101	70-71	0.681659754710843
1101	72-73	0.6578543324582427
1101	74-75	0.6109386842503071
1101	76-77	0.63637749640408
1101	78-79	0.5730106995996707
1101	80-81	0.6189365619522178
1101	82-83	0.6162735677755364
1101	84-85	0.6125161969507289
1101	86-87	0.6068118663508706
1101	88-89	0.6010307555549943
1101	90-91	0.6176499039607002
1101	92-93	0.5633099991146544
1101	94-95	0.574815324717683
1101	96-97	0.5324274319369877
1101	98-99	0.573352985963286
1101	100	0.5687914654273811
1102	1	0.24216664990717973
1102	2	0.32365421649668136
1102	3	0.3337851568928478
1102	4	0.30963650387616326
1102	5	0.2888085744114264
1102	6	0.3325324892246897
1102	7	0.3697488632151362
1102	8	0.3806427628711333
1102	9	0.4763076527059411
1102	10-11	0.46387693775677974
1102	12-13	0.48457146106930793
1102	14-15	0.5889301161406806
1102	16-17	0.5359882814249772
1102	18-19	0.5383246467736171
1102	20-21	0.5274735701852507
1102	22-23	0.5310818733489384
1102	24-25	0.5765089435456332
1102	26-27	0.5606800818590898
1102	28-29	0.6074253313178346
1102	30-31	0.56318947786405
1102	32-33	0.5565550155258876
1102	34-35	0.5895055782769134
1102	36-37	0.6076575604249328
1102	38-39	0.6030185367576664
1102	40-41	0.6138536550606801
1102	42-43	0.5746485149437532
1102	44-45	0.5146792898986661
1102	46-47	0.4927739220845808
1102	48-49	0.49769982040495364
1102	50-51	0.546372091008088
1102	52-53	0.524657638055821
1102	54-55	0.5140437545065168
1102	56-57	0.5349449958831727
1102	58-59	0.5828229701403771
1102	60-61	0.5083127906152427
1102	62-63	0.4441558797575951
1102	64-65	0.5000456794359565
1102	66-67	0.4913975848423888
1102	68-69	0.5035050724522776
1102	70-71	0.4630667134748023
1102	72-73	0.49302138569980514
1102	74-75	0.5115159540629364
1102	76-77	0.4482182539957762
1102	78-79	0.4077613602831818
1102	80-81	0.44875597116119437
1102	82-83	0.4275107812391603
1102	84-85	0.4077441587650945
1102	86-87	0.3750443321638066
1102	88-89	0.3697867911448398
1102	90-91	0.429468354012311
1102	92-93	0.3758050146459908
1102	94-95	0.4133288506788162
1102	96-97	0.4078696085348561
1102	98-99	0.4496608852838513
1102	100	0.41985392237969776
1103	1	0.27897858545455634
1103	2	0.3318557984466608
1103	3	0.29600099830554427
1103	4	0.3009964056099079
1103	5	0.29708106452729055
1103	6	0.33305014181114956
1103	7	0.34305725184645297
1103	8	0.33997339725222986
1103	9	0.4086934281660035
1103	10-11	0.45750191598389733
1103	12-13	0.4931535318146345
1103	14-15	0.5725297954600137
1103	16-17	0.5011260404253903
1103	18-19	0.4907766505754836
1103	20-21	0.49097179683491277
1103	22-23	0.5169232946598967
1103	24-25	0.5460360300198488
1103	26-27	0.5571527452390157
1103	28-29	0.4992300245538601
1103	30-31	0.5283874685983179
1103	32-33	0.5694713515007876
1103	34-35	0.5449472056207583
1103	36-37	0.5473290709512284
1103	38-39	0.5611594920822185
1103	40-41	0.5835097633798654
1103	42-43	0.5586643166375325
1103	44-45	0.5224514093190393
1103	46-47	0.49296482702815325
1103	48-49	0.46990238340919177
1103	50-51	0.45760369031750514
1103	52-53	0.4472366404218704
1103	54-55	0.43240293839919275
1103	56-57	0.43335832156375176
1103	58-59	0.40226030853099815
1103	60-61	0.43726420128997745
1103	62-63	0.4322126643034565
1103	64-65	0.427024585189983
1103	66-67	0.39937971477837664
1103	68-69	0.3854169134123566
1103	70-71	0.3139418147285937
1103	72-73	0.3893866313934353
1103	74-75	0.379793086667668
1103	76-77	0.4034338454062123
1103	78-79	0.3381228641279179
1103	80-81	0.34799053488570664
1103	82-83	0.31885938907406697
1103	84-85	0.3441257630214203
1103	86-87	0.35581940235757514
1103	88-89	0.3277350250246158
1103	90-91	0.30889328275592476
1103	92-93	0.3043209460796028
1103	94-95	0.3585857362594851
1103	96-97	0.36217796169772853
1103	98-99	0.304277189250989
1103	100	0.28763842416367424
1104	1	0.17097213370913522
1104	2	0.14786279050661477
1104	3	0.17069498427756002
1104	4	0.21536323670347457
1104	5	0.27033366400765857
1104	6	0.2856319087719612
1104	7	0.26599354799730435
1104	8	0.29904076284638137
1104	9	0.3226546195177633
1104	10-11	0.2964818320889222
1104	12-13	0.31243329811467646
1104	14-15	0.39779820679950717
1104	16-17	0.4203926852984381
1104	18-19	0.38622495671380364
1104	20-21	0.36359832774058987
1104	22-23	0.3470278274183798
1104	24-25	0.38980048003918455
1104	26-27	0.39242517064201365
1104	28-29	0.3528459960007453
1104	30-31	0.3091943789148033
1104	32-33	0.35067119093824317
1104	34-35	0.3798257201457176
1104	36-37	0.3712786448442884
1104	38-39	0.40167251585570085
1104	40-41	0.39988491782382596
1104	42-43	0.4071906609745568
1104	44-45	0.3377365766755531
1104	46-47	0.280109090659856
1104	48-49	0.25110638661069373
1104	50-51	0.25727827266168646
1104	52-53	0.24259442298774303
1104	54-55	0.29571218038873326
1104	56-57	0.26841549498765715
1104	58-59	0.3906443363878367
1104	60-61	0.3378028656338543
1104	62-63	0.3668927594573077
1104	64-65	0.4003741488477033
1104	66-67	0.36963478014006057
1104	68-69	0.324469918198254
1104	70-71	0.3729832612397104
1104	72-73	0.3756644700378331
1104	74-75	0.3344022167995675
1104	76-77	0.35047993333056127
1104	78-79	0.31467498898792456
1104	80-81	0.38993452181511046
1104	82-83	0.37498675778059365
1104	84-85	0.338566835141922
1104	86-87	0.3342452593322811
1104	88-89	0.33000991979035277
1104	90-91	0.3401003809291936
1104	92-93	0.30391015385386666
1104	94-95	0.3752836485635136
1104	96-97	0.4282201786444588
1104	98-99	0.42326665833974175
1104	100	0.3375366730502307
1105	1	0.18469902339712263
1105	2	0.22212675153640404
1105	3	0.15351578426464485
1105	4	0.19977252885495034
1105	5	0.21479037744988005
1105	6	0.2576552440828621
1105	7	0.2587741710525293
1105	8	0.2679768308747441
1105	9	0.3239313217403108
1105	10-11	0.3730556135635439
1105	12-13	0.35162818549415675
1105	14-15	0.4263857182512538
1105	16-17	0.34993208331037806
1105	18-19	0.34830077831288975
1105	20-21	0.3614052242088164
1105	22-23	0.3780820228909647
1105	24-25	0.37581715215425504
1105	26-27	0.3481340886697737
1105	28-29	0.3812070354754411
1105	30-31	0.37045380767995795
1105	32-33	0.4107403957538338
1105	34-35	0.416793057974985
1105	36-37	0.3924648329393321
1105	38-39	0.39399939787556804
1105	40-41	0.4387509940449803
1105	42-43	0.44289438283960436
1105	44-45	0.44395685765174875
1105	46-47	0.39056712988029574
1105	48-49	0.40483404311103754
1105	50-51	0.3759950828029801
1105	52-53	0.4120987090652548
1105	54-55	0.3912307372478665
1105	56-57	0.37882491225062154
1105	58-59	0.3773082889423236
1105	60-61	0.3497739063596583
1105	62-63	0.357042358495157
1105	64-65	0.35149903231240387
1105	66-67	0.3482877127937627
1105	68-69	0.38685879094372666
1105	70-71	0.3833749711909178
1105	72-73	0.31553533278245993
1105	74-75	0.37803735133477545
1105	76-77	0.33557594630987353
1105	78-79	0.2979591857923367
1105	80-81	0.3330423921350345
1105	82-83	0.34794657277702257
1105	84-85	0.3335616389792122
1105	86-87	0.29618784728364034
1105	88-89	0.28693921969066594
1105	90-91	0.2949883052488502
1105	92-93	0.29316984696748705
1105	94-95	0.2781571769900566
1105	96-97	0.3090389425429336
1105	98-99	0.3031210144393306
1105	100	0.26295511044807185
1106	1	0.1823745077137211
1106	2	0.19530612612599896
1106	3	0.1846591675180811
1106	4	0.23247862141464282
1106	5	0.26505959462632234
1106	6	0.2877043341902379
1106	7	0.219854286276572
1106	8	0.2436549460121995
1106	9	0.2974496894215548
1106	10-11	0.2885977023987252
1106	12-13	0.3279111346457384
1106	14-15	0.395598639206419
1106	16-17	0.4036820756903552
1106	18-19	0.42126293550783345
1106	20-21	0.451962627325571
1106	22-23	0.4780490438454663
1106	24-25	0.4626591519094774
1106	26-27	0.45110037786812285
1106	28-29	0.4422792102503479
1106	30-31	0.4491193526800217
1106	32-33	0.4309582270483574
1106	34-35	0.4407876337728993
1106	36-37	0.4645780908634052
1106	38-39	0.4388456145876205
1106	40-41	0.4753557318515078
1106	42-43	0.4463814494423062
1106	44-45	0.3968849412540436
1106	46-47	0.36932684852892805
1106	48-49	0.3834556929019044
1106	50-51	0.3631173995603163
1106	52-53	0.3737053381992297
1106	54-55	0.3961601364168956
1106	56-57	0.32656114569232386
1106	58-59	0.27733991983086526
1106	60-61	0.3003226394594307
1106	62-63	0.321058105046653
1106	64-65	0.36729887508214176
1106	66-67	0.40153825209561944
1106	68-69	0.38359280254459094
1106	70-71	0.37608000593585444
1106	72-73	0.35873515676395584
1106	74-75	0.3725556669654466
1106	76-77	0.31909144495678987
1106	78-79	0.27756788186586334
1106	80-81	0.29040114475299816
1106	82-83	0.329379403376759
1106	84-85	0.3408000011802983
1106	86-87	0.33113495771825185
1106	88-89	0.3437929722484512
1106	90-91	0.3171550732812882
1106	92-93	0.29196804318817726
1106	94-95	0.32637485096052643
1106	96-97	0.34917000958142097
1106	98-99	0.38851232891660104
1106	100	0.3600915195487673
1107	1	0.2860985537803309
1107	2	0.30423333773266137
1107	3	0.30444409042313936
1107	4	0.309974205651514
1107	5	0.32296190133659763
1107	6	0.3892208992434263
1107	7	0.3271876193047234
1107	8	0.3333057141005398
1107	9	0.3706355033452553
1107	10-11	0.349501434833428
1107	12-13	0.37662379597434636
1107	14-15	0.45651266192752615
1107	16-17	0.4418842155969003
1107	18-19	0.4657452400681166
1107	20-21	0.4233111320697063
1107	22-23	0.4664687522597575
1107	24-25	0.4312176061913533
1107	26-27	0.4749371921221055
1107	28-29	0.5151998323352558
1107	30-31	0.49179721403387333
1107	32-33	0.47756901869963997
1107	34-35	0.5220725992887196
1107	36-37	0.4497664204617138
1107	38-39	0.4540002309569715
1107	40-41	0.5492197128430689
1107	42-43	0.5181143302395483
1107	44-45	0.4923917310882828
1107	46-47	0.4781480577876849
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2214	100	-0.30703388024728895
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2216	2	-1.0249205352059043
2216	3	-1.082879270166984
2216	4	-1.2033603469801122
2216	5	-1.1218993785291218
2216	6	-1.6384233698413269
2216	7	-1.5359997359255857
2216	8	-1.7806660065333801
2216	9	-1.6884435443392434
2216	10-11	-1.5314686363400938
2216	12-13	-1.4716820026750455
2216	14-15	-1.6553411215181484
2216	16-17	-1.8135871601554356
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2216	28-29	-1.9367628652714188
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2216	32-33	-1.991819047672223
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2216	36-37	-2.032334919231836
2216	38-39	-1.9457283410626829
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2216	50-51	-2.045548915079337
2216	52-53	-2.2050626885250324
2216	54-55	-2.0107730821591403
2216	56-57	-1.983018679390959
2216	58-59	-2.2985412179899143
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2216	62-63	-2.180633477820166
2216	64-65	-2.1592021998142314
2216	66-67	-2.071793288974849
2216	68-69	-2.147024832337003
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2216	74-75	-2.405437737458314
2216	76-77	-2.546195588408281
2216	78-79	-2.255363953237108
2216	80-81	-2.223806526596732
2216	82-83	-2.3137579546505798
2216	84-85	-2.2392425893530152
2216	86-87	-2.213155465549381
2216	88-89	-2.206705014913176
2216	90-91	-2.28928132262595
2216	92-93	-2.2695872064161087
2216	94-95	-2.2100245380870547
2216	96-97	-2.3125452641278095
2216	98-99	-2.2942834861974255
2216	100	-2.251840604404009
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	180805.0
3	29696.0
4	19245.0
5	15802.0
6	14273.0
7	15026.0
8	15710.0
9	18265.0
10	19924.0
11	21964.0
12	23844.0
13	25110.0
14	26820.0
15	28430.0
16	29474.0
17	30922.0
18	29905.0
19	28204.0
20	28499.0
21	29973.0
22	33391.0
23	39317.0
24	47336.0
25	57870.0
26	69767.0
27	82583.0
28	100344.0
29	123992.0
30	148705.0
31	179987.0
32	228595.0
33	298734.0
34	410598.0
35	603508.0
36	1014874.0
37	1734816.0
38	1911614.0
39	542365.0
40	918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.666352091492925	18.56754528901473	13.014473929945822	23.751628689546518
2	16.694952169694616	19.64122488778819	38.347926457530704	25.31589648498649
3	18.677983626286082	24.826407638160777	29.435842984752252	27.059765750800892
4	13.390121993519264	16.66429172873891	37.07351149201591	32.87207478572592
5	14.13071599958692	36.37567590046716	33.99171443455719	15.501893665388728
6	31.72289213803844	36.06981340123262	18.006686621285326	14.200607839443608
7	28.408895264380995	31.260008286310175	21.74145453074955	18.589641918559277
8	26.528903247770035	34.28756589280928	20.133677868999126	19.049852990421556
9	25.7300411355235	16.88512876612558	20.54394823059622	36.8408818677547
10-11	24.92867396068528	26.038231156598997	27.842437478521674	21.190657404194045
12-13	25.372828308473274	23.519604321402657	27.8375383022466	23.27002906787747
14-15	22.89897454259079	25.478171991492708	25.037583357571712	26.585270108344794
16-17	22.920849532100192	28.505162539734197	25.40770528607652	23.166282642089094
18-19	22.9099827597802	27.78426583727656	26.942993231695876	22.362758171247364
20-21	23.885321034551914	27.081105042822827	26.516762505480617	22.516811417144638
22-23	23.601241561122368	26.9928947714901	26.482129480115198	22.923734187272334
24-25	23.32169913424827	27.041168139207954	26.520374437305566	23.116758289238213
26-27	23.197100099179416	27.009705781854315	26.53326323887833	23.25993088008794
28-29	23.22995185358871	27.048910160577428	26.6254733178372	23.095664667996665
30-31	23.349670696245507	27.36318013443821	26.32091659046613	22.966232578850153
32-33	23.088501258399088	27.11617738206137	26.63055404003402	23.164767319505522
34-35	23.243872677946847	26.774644062724235	26.7103772318302	23.27110602749872
36-37	23.530796885638548	26.986863043059273	26.445450248763446	23.036889822538733
38-39	23.316757179003154	27.129226264052587	26.472762365965092	23.081254190979163
40-41	23.284033742651598	26.97315243062166	26.604654006463175	23.13815982026357
42-43	23.104776486550076	27.08079844404584	26.605134622104792	23.209290447299292
44-45	23.20875614021409	27.143711398920324	26.53013190654525	23.11740055432034
46-47	23.315579524327706	27.10654561532736	26.385012608303803	23.19286225204113
48-49	23.230091375020166	26.971415530393717	26.533811844762045	23.26468124982407
50-51	23.164977869916342	26.989028965173585	26.532323215080638	23.31366994982944
52-53	23.212892169052335	26.96491002082077	26.504420932535044	23.31777687759185
54-55	23.238950437190717	27.236424514980623	26.46350854154324	23.061116506285416
56-57	23.005965839439916	27.055641819596165	26.483084681019793	23.455307659944125
58-59	23.085027914173224	26.69964005806429	26.64808748236638	23.567244545396107
60-61	23.274260399908428	27.021390780244793	26.43553620287834	23.26881261696844
62-63	23.063367098408037	27.178567677601094	26.441313269411033	23.316751954579836
64-65	23.162656056270002	26.852475333775942	26.54265565793215	23.4422129520219
66-67	22.977277282385323	26.921393405613436	26.49401185806281	23.60731745393843
68-69	23.29971106630402	27.04812500609121	26.359468724233427	23.292695203371345
70-71	23.280259602782753	26.80476342023748	26.498052149754574	23.41692482722519
72-73	23.074222712024568	26.96908161353485	26.611587107920602	23.34510856651998
74-75	23.051651130387008	26.94074856603671	26.549546321504526	23.458053982071757
76-77	23.1766034304044	26.997334425809555	26.32644337246829	23.499618771317753
78-79	23.1870055669898	27.051701207604644	26.495486487064003	23.265806738341553
80-81	23.0856248930783	26.935835116421003	26.56108705208965	23.417452938411042
82-83	23.098545305833472	26.926557545031983	26.585601871569768	23.38929527756477
84-85	23.14060287534552	26.948622133419597	26.399664359003662	23.511110632231222
86-87	23.251722926608625	27.043568899902727	26.387062839551096	23.317645333937552
88-89	23.1038094970016	26.914153974951628	26.5252652373978	23.456771290648977
90-91	23.005455407352233	26.86808551286404	26.516956582352343	23.609502497431382
92-93	23.168749755227182	27.091682469835366	26.354649194472707	23.38491858046475
94-95	23.22155006142428	26.821344631801182	26.46316244182793	23.49394286494661
96-97	23.13361474578998	26.876420999136492	26.497211479294226	23.492752775779305
98-99	23.076199070570464	26.89467189570904	26.506597506724187	23.522531526996303
100	23.255969914916623	26.79396448641167	26.365714955310832	23.584350643360878
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2132.0
1	1626.0
2	1753.0
3	2194.5
4	1796.0
5	1518.5
6	1342.0
7	1233.5
8	1314.5
9	1324.5
10	1180.0
11	1096.5
12	1149.0
13	1247.5
14	1319.5
15	1482.5
16	1721.5
17	2034.5
18	2539.0
19	3290.5
20	4302.5
21	5783.5
22	8201.5
23	12003.5
24	17134.0
25	23916.0
26	33265.5
27	45486.0
28	60934.0
29	77511.5
30	93002.5
31	108809.0
32	124741.5
33	139656.0
34	154887.0
35	171525.0
36	188413.5
37	207015.0
38	225738.0
39	239214.0
40	251936.0
41	263640.5
42	271075.0
43	277512.5
44	281890.5
45	285472.0
46	288695.0
47	287310.0
48	284042.0
49	285070.0
50	287293.5
51	285082.0
52	277516.0
53	272424.0
54	269132.5
55	259477.5
56	244974.0
57	225715.0
58	206322.0
59	192123.5
60	175368.0
61	153309.5
62	132147.5
63	111628.0
64	91943.5
65	75208.5
66	59476.0
67	45521.0
68	35040.0
69	27030.5
70	20579.5
71	15681.0
72	12362.0
73	9391.0
74	7095.0
75	5255.5
76	3608.0
77	2609.5
78	1922.5
79	1349.5
80	942.5
81	669.5
82	490.5
83	368.0
84	304.0
85	263.0
86	207.5
87	151.0
88	138.5
89	135.5
90	113.0
91	78.5
92	55.5
93	45.5
94	33.5
95	26.0
96	23.5
97	21.5
98	24.0
99	28.0
100	51.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.055282492081966256
2	0.01669248009218994
3	0.019670253915742314
4	0.016220393998212124
5	0.01571199358931294
6	0.013666287182075739
7	0.0217764841811818
8	0.014513621196907713
9	0.01441678302378406
10-11	0.01723114243019027
12-13	0.019028701018798104
14-15	0.014919131046863017
16-17	0.015941984250481617
18-19	0.01403548271710967
20-21	0.01714640902870707
22-23	0.01746113309135895
24-25	0.018211628933067273
26-27	0.011166651838321407
28-29	0.015681731660211796
30-31	0.014549935511829086
32-33	0.015802779376616366
34-35	0.013460506064187971
36-37	0.013908382614884874
38-39	0.01996682082093351
40-41	0.016129608210908697
42-43	0.012885529411266273
44-45	0.02083231199322617
46-47	0.018992386703876735
48-49	0.018913705688213763
50-51	0.014307840079019949
52-53	0.014319944850660406
54-55	0.015149121708031699
56-57	0.02033601635596744
58-59	0.008545968778162508
60-61	0.011668999861400364
62-63	0.017400609233156665
64-65	0.022811442156440858
66-67	0.015197540794593525
68-69	0.01635959887207738
70-71	0.0238645572891606
72-73	0.019821563561248024
74-75	0.020172601938821273
76-77	0.012964210426929244
78-79	0.014229159063356981
80-81	0.020045501836596476
82-83	0.014368363937222232
84-85	0.014392573480503148
86-87	0.018090581216662703
88-89	0.016795370651133824
90-91	0.017170618571987983
92-93	0.012038195396434297
94-95	0.01745508070553872
96-97	0.008860692840814385
98-99	0.006615257701509647
100	0.0044424511920476495
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8261205.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.42718496139037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63959311644778	41.37062533108271
2	14.34916166284072	15.906672750543288
3	4.59022007262793	7.632689309371051
4	2.04010345684821	4.523087665723905
5	1.1388567036456807	3.1561810578744236
6	0.691898178064702	2.300998097402475
7	0.47840022342025756	1.8561464368359566
8	0.3360504291042102	1.4901063432250927
9	0.2738164767575849	1.3659188852447015
>10	1.3402479939020486	13.293940201770631
>50	0.08406711794290371	3.1893842428139223
>100	0.036444713930828244	3.4199065395003068
>500	9.426503679704269E-4	0.3331551886891096
>1k	1.9720409928134522E-4	0.1611879499224502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.420954328091362E-5	0.0	1.210477164045681E-5	0.0
4	0.0	2.420954328091362E-5	0.0	2.420954328091362E-5	0.0
5	0.0	6.052385820228405E-5	0.0	2.420954328091362E-5	0.0
6	0.0	6.052385820228405E-5	0.0	3.631431492137043E-5	0.0
7	0.0	6.052385820228405E-5	0.0	3.631431492137043E-5	0.0
8	0.0	6.052385820228405E-5	0.0	3.631431492137043E-5	0.0
9	0.0	6.052385820228405E-5	0.0	8.473340148319767E-5	0.0
10-11	0.0	6.657624402251245E-5	0.0	1.271001022247965E-4	0.0
12-13	0.0	8.473340148319767E-5	0.0	1.5736203132593854E-4	0.0
14-15	0.0	1.0894294476411129E-4	0.0	1.9367634624730895E-4	0.0
16-17	0.0	1.392048738652533E-4	0.0	2.0578111788776578E-4	0.0
18-19	0.0	1.4525725968548173E-4	0.0	2.2393827534845099E-4	0.0
20-21	0.0	1.4525725968548173E-4	0.0	2.481478186293646E-4	0.0
22-23	0.0	1.4525725968548173E-4	0.0	3.510383775732475E-4	0.0
24-25	6.052385820228405E-6	1.6946680296639534E-4	0.0	7.747053849892359E-4	1.210477164045681E-5
26-27	1.210477164045681E-5	1.9367634624730895E-4	1.210477164045681E-5	0.001301262951349107	1.210477164045681E-5
28-29	1.210477164045681E-5	1.9367634624730895E-4	2.420954328091362E-5	0.0028022546347657514	1.210477164045681E-5
30-31	1.210477164045681E-5	1.9972873206753738E-4	2.420954328091362E-5	0.007692582377510302	1.210477164045681E-5
32-33	1.210477164045681E-5	2.0578111788776578E-4	2.420954328091362E-5	0.018423462436775263	1.210477164045681E-5
34-35	1.210477164045681E-5	2.1183350370799418E-4	2.420954328091362E-5	0.03425045135667254	1.210477164045681E-5
36-37	1.210477164045681E-5	2.360430469889078E-4	2.420954328091362E-5	0.06022729129709286	1.210477164045681E-5
38-39	1.210477164045681E-5	2.54200204449593E-4	2.420954328091362E-5	0.09781865962653148	1.210477164045681E-5
40-41	1.210477164045681E-5	2.54200204449593E-4	2.420954328091362E-5	0.14174082352392903	1.210477164045681E-5
42-43	1.210477164045681E-5	2.663049760900498E-4	2.420954328091362E-5	0.18755133179723782	1.210477164045681E-5
44-45	1.210477164045681E-5	2.663049760900498E-4	3.0261929101142024E-5	0.2416536086442595	1.210477164045681E-5
46-47	1.210477164045681E-5	2.663049760900498E-4	4.841908656182724E-5	0.31045107826279583	1.210477164045681E-5
48-49	1.210477164045681E-5	2.663049760900498E-4	4.841908656182724E-5	0.38437491867106555	1.210477164045681E-5
50-51	1.210477164045681E-5	2.784097477305066E-4	4.841908656182724E-5	0.46238411950798947	1.210477164045681E-5
52-53	1.210477164045681E-5	2.784097477305066E-4	4.841908656182724E-5	0.542027464516375	1.210477164045681E-5
54-55	1.210477164045681E-5	2.784097477305066E-4	4.841908656182724E-5	0.6313788363804069	1.210477164045681E-5
56-57	1.210477164045681E-5	2.784097477305066E-4	4.841908656182724E-5	0.7351469912682229	1.210477164045681E-5
58-59	1.210477164045681E-5	2.905145193709634E-4	4.841908656182724E-5	0.8511712274420016	1.210477164045681E-5
60-61	1.210477164045681E-5	3.0261929101142027E-4	4.841908656182724E-5	0.9724973535943	1.210477164045681E-5
62-63	1.210477164045681E-5	3.0261929101142027E-4	4.841908656182724E-5	1.0984898692139948	1.210477164045681E-5
64-65	1.210477164045681E-5	3.0867167683164867E-4	4.841908656182724E-5	1.235969813120483	1.210477164045681E-5
66-67	1.210477164045681E-5	3.1472406265187707E-4	4.841908656182724E-5	1.392629767691275	1.210477164045681E-5
68-69	1.210477164045681E-5	3.1472406265187707E-4	4.841908656182724E-5	1.5638457101597165	1.210477164045681E-5
70-71	1.210477164045681E-5	3.1472406265187707E-4	4.841908656182724E-5	1.7443641696338488	1.210477164045681E-5
72-73	1.210477164045681E-5	3.1472406265187707E-4	4.841908656182724E-5	1.9398864935563274	1.210477164045681E-5
74-75	1.210477164045681E-5	3.207764484721055E-4	5.4471472382055646E-5	2.1367161328159754	1.210477164045681E-5
76-77	1.210477164045681E-5	3.268288342923339E-4	6.052385820228405E-5	2.3487796271851384	1.210477164045681E-5
78-79	1.210477164045681E-5	3.268288342923339E-4	6.052385820228405E-5	2.584211383206203	1.210477164045681E-5
80-81	1.210477164045681E-5	3.268288342923339E-4	6.052385820228405E-5	2.8383813257266945	1.210477164045681E-5
82-83	1.210477164045681E-5	3.328812201125623E-4	6.052385820228405E-5	3.107615656553735	1.210477164045681E-5
84-85	1.210477164045681E-5	3.631431492137043E-4	6.052385820228405E-5	3.374283775792999	1.210477164045681E-5
86-87	1.210477164045681E-5	3.813003066743895E-4	6.052385820228405E-5	3.6556410354179567	1.210477164045681E-5
88	1.210477164045681E-5	3.873526924946179E-4	6.052385820228405E-5	3.884760153028523	1.210477164045681E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	840	0.0	35.81047	2
GGTATCA	7900	0.0	35.1158	1
GTATCAA	12000	0.0	32.991985	1
TACCTGG	7535	0.0	31.375744	2
GTACCTG	8105	0.0	30.050682	1
TCAACGC	12740	0.0	29.513762	4
ATCAACG	12825	0.0	29.208565	3
ATAACGC	1190	0.0	27.647787	3
CAACGCA	13760	0.0	27.463764	5
TAACGCA	1240	0.0	27.29071	4
AACGCAG	14695	0.0	25.907766	6
TATCAAC	14855	0.0	25.75497	2
GTACATG	25555	0.0	22.999147	1
TATCACG	1210	0.0	22.917957	2
ACCTGGG	10365	0.0	22.264938	3
ACGCAGA	17155	0.0	22.138105	7
TACATGG	25885	0.0	21.934517	2
GTATAAC	2305	0.0	21.41884	1
CGCAGAG	17925	0.0	21.081345	8
ACATGGG	25970	0.0	20.921614	3
>>END_MODULE