FastQCFastQC Report
Wed 25 May 2016
SRR2049484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8261205
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153300.1855661492482029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144030.17434502593749943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117400.049.0525931
TATAACG10800.040.0395622
GTATCAA193650.036.2007371
TAACGCA13200.033.8278924
ATAACGC13650.032.0239943
TACCTGG75350.030.3164422
ATCAACG225500.030.3070133
TCAACGC227000.030.1688634
GTACCTG81100.029.7608431
CAACGCA236900.028.9874735
TATCAAC242450.028.3239142
AACGCAG250850.027.715926
ACGCAGA287350.024.2280837
TATCACG13050.023.7716122
CGCAGAG295400.023.5837528
ACCTGGG96600.023.1122253
GTACATG247400.022.5355071
GTATAAC27800.022.1704851
TACATGG256700.020.9837932
GCAGAGT330700.020.8815549
Produced by FastQC (version 0.11.5)