##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049484_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8261205 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.879246066403145 33.0 31.0 34.0 30.0 34.0 2 32.04602113129985 33.0 31.0 34.0 30.0 34.0 3 32.00093158322545 34.0 31.0 34.0 30.0 34.0 4 35.74094057707078 37.0 35.0 37.0 33.0 37.0 5 35.783281978839646 37.0 35.0 37.0 35.0 37.0 6 35.817981880367334 37.0 35.0 37.0 35.0 37.0 7 35.817108763188905 37.0 35.0 37.0 35.0 37.0 8 35.81865660033857 37.0 35.0 37.0 35.0 37.0 9 37.55243078945505 39.0 37.0 39.0 35.0 39.0 10-11 37.51126802930081 39.0 37.0 39.0 35.0 39.0 12-13 37.479072847120975 39.0 37.0 39.0 35.0 39.0 14-15 38.78736104478705 40.0 38.0 41.0 35.0 41.0 16-17 38.78948204287389 40.0 38.0 41.0 35.0 41.0 18-19 38.83612753829496 40.0 38.0 41.0 35.0 41.0 20-21 38.842671680463084 40.0 38.0 41.0 35.0 41.0 22-23 38.80799235704718 40.0 38.0 41.0 35.0 41.0 24-25 38.78086296127502 40.0 38.0 41.0 35.0 41.0 26-27 38.67764769183188 40.0 38.0 41.0 34.0 41.0 28-29 38.59819971783777 40.0 38.0 41.0 34.0 41.0 30-31 38.51269851068942 40.0 38.0 41.0 34.0 41.0 32-33 38.406021579176404 40.0 38.0 41.0 34.0 41.0 34-35 38.30318173922569 40.0 38.0 41.0 34.0 41.0 36-37 38.1949262849669 40.0 38.0 41.0 34.0 41.0 38-39 38.10953571543135 40.0 38.0 41.0 33.0 41.0 40-41 38.028283888367376 40.0 38.0 41.0 33.0 41.0 42-43 37.90489220398235 40.0 37.0 41.0 33.0 41.0 44-45 37.752341637811924 40.0 37.0 41.0 33.0 41.0 46-47 37.55262277113327 40.0 37.0 41.0 33.0 41.0 48-49 37.35246377495777 40.0 37.0 41.0 32.0 41.0 50-51 37.18988380024464 40.0 36.0 41.0 32.0 41.0 52-53 37.04680564154987 40.0 36.0 41.0 32.0 41.0 54-55 36.87450335635056 39.0 35.5 41.0 31.0 41.0 56-57 36.69447108502936 39.0 35.0 41.0 31.0 41.0 58-59 36.47787925611336 39.0 35.0 41.0 31.0 41.0 60-61 36.24965922041639 38.5 35.0 40.5 31.0 41.0 62-63 35.99666471174605 38.0 35.0 40.0 30.5 41.0 64-65 35.74164132230105 37.5 35.0 40.0 30.0 41.0 66-67 35.44376643601024 37.0 35.0 40.0 30.0 41.0 68-69 35.12588962506075 36.5 34.5 39.5 30.0 41.0 70-71 34.80969386427283 36.0 34.0 39.0 29.0 41.0 72-73 34.47659602927176 35.5 34.0 39.0 29.0 40.5 74-75 34.160623298901314 35.0 34.0 37.5 29.0 40.0 76-77 33.85415929032145 35.0 34.0 37.0 29.0 39.0 78-79 33.56349128244608 35.0 34.0 37.0 29.0 39.0 80-81 33.28482763713042 35.0 34.0 36.0 29.0 39.0 82-83 33.03882714446621 35.0 33.5 36.0 29.0 37.0 84-85 32.79759423716032 35.0 33.0 35.5 28.5 37.0 86-87 32.59582022235255 35.0 33.0 35.0 28.0 37.0 88-89 32.409360862005 35.0 33.0 35.0 27.0 36.0 90-91 32.244623272270815 35.0 33.0 35.0 27.0 36.0 92-93 32.0953129113731 35.0 33.0 35.0 27.0 36.0 94-95 31.947146148776117 35.0 33.0 35.0 27.0 35.5 96-97 31.794484460802025 35.0 33.0 35.0 26.5 35.0 98-99 31.656641736889476 35.0 33.0 35.0 26.0 35.0 100 31.546678601971504 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25280217389806126 1101 2 0.2855079137610872 1101 3 0.42691886372094956 1101 4 0.23519221213637564 1101 5 0.20823231626470573 1101 6 0.20694396318285868 1101 7 0.18899886943744804 1101 8 0.24102837255470888 1101 9 0.295495484713598 1101 10-11 0.27221002629774205 1101 12-13 0.23466484771542184 1101 14-15 0.3496324045317607 1101 16-17 0.33210961835938235 1101 18-19 0.3744443097653374 1101 20-21 0.3752607637272547 1101 22-23 0.35841528622069063 1101 24-25 0.3647519960730179 1101 26-27 0.3809152675574765 1101 28-29 0.39268572841131544 1101 30-31 0.4136327683857175 1101 32-33 0.41676112072970994 1101 34-35 0.42029315128944233 1101 36-37 0.43399230553020374 1101 38-39 0.38002643345370757 1101 40-41 0.3740730476362373 1101 42-43 0.4082923630971038 1101 44-45 0.397055145525016 1101 46-47 0.39376721895775546 1101 48-49 0.40529387986024545 1101 50-51 0.36005856897112665 1101 52-53 0.36602738874940144 1101 54-55 0.32353078034424243 1101 56-57 0.37918438459659853 1101 58-59 0.3577293549999965 1101 60-61 0.32169220428907863 1101 62-63 0.34552249804522717 1101 64-65 0.3059526712907683 1101 66-67 0.3437377874553178 1101 68-69 0.382410660368933 1101 70-71 0.38249609975139265 1101 72-73 0.3609539323167681 1101 74-75 0.39119385673791385 1101 76-77 0.4034623214926114 1101 78-79 0.3867366357331008 1101 80-81 0.371837254494082 1101 82-83 0.34542179589865185 1101 84-85 0.3535764373520891 1101 86-87 0.3172113590585326 1101 88-89 0.38772491740959936 1101 90-91 0.3610647434838512 1101 92-93 0.40084623342716696 1101 94-95 0.3625101354405551 1101 96-97 0.31479993142792395 1101 98-99 0.37277253599819105 1101 100 0.3844617463838631 1102 1 0.2373700806351593 1102 2 0.23419782305713 1102 3 0.35758380521175326 1102 4 0.24665106731609399 1102 5 0.19306342671815457 1102 6 0.14239017638322338 1102 7 0.17900651773690868 1102 8 0.1659231118677411 1102 9 0.19871464920076676 1102 10-11 0.24683455689599043 1102 12-13 0.2239857313168585 1102 14-15 0.3314917019697958 1102 16-17 0.29444156525272547 1102 18-19 0.29663728878094986 1102 20-21 0.308624419863996 1102 22-23 0.27519954474866637 1102 24-25 0.2985153679687471 1102 26-27 0.30902012354421515 1102 28-29 0.2785443376806782 1102 30-31 0.2678021315682244 1102 32-33 0.2616335955874831 1102 34-35 0.2760228017318411 1102 36-37 0.3036128901526709 1102 38-39 0.2392438822733851 1102 40-41 0.27014679429864685 1102 42-43 0.2549508971314438 1102 44-45 0.23693124396522336 1102 46-47 0.2406503883607627 1102 48-49 0.17443048817520435 1102 50-51 0.18454698866131025 1102 52-53 0.2010774369746997 1102 54-55 0.20263704654190917 1102 56-57 0.19480937159686817 1102 58-59 0.14348684863978178 1102 60-61 0.13157151774871068 1102 62-63 0.15164436897259037 1102 64-65 0.12732807116252332 1102 66-67 0.1188564490088524 1102 68-69 0.1164532590792362 1102 70-71 0.09947709250897674 1102 72-73 0.11756240178329591 1102 74-75 0.0758363596513405 1102 76-77 0.08306846824130787 1102 78-79 0.10323115623464219 1102 80-81 0.14145039584142438 1102 82-83 0.12817162697641749 1102 84-85 0.0878709485829674 1102 86-87 0.05247172066204797 1102 88-89 0.06619962691387826 1102 90-91 0.11847122564049073 1102 92-93 0.12499908175015406 1102 94-95 0.08089323471928722 1102 96-97 0.07935217311656473 1102 98-99 0.08324874515154335 1102 100 0.14502476403319164 1103 1 0.2023351571428904 1103 2 0.17154533208830003 1103 3 0.2957046698893038 1103 4 0.19558570012509335 1103 5 0.19147533078908197 1103 6 0.15827413684983327 1103 7 0.1788476133700243 1103 8 0.16502913479701675 1103 9 0.2147900530994491 1103 10-11 0.21452704894466734 1103 12-13 0.21772868878474583 1103 14-15 0.29655443875215326 1103 16-17 0.25621610456863664 1103 18-19 0.26897141529588 1103 20-21 0.2854499066540086 1103 22-23 0.28219267952286486 1103 24-25 0.29930074985833244 1103 26-27 0.30359085271652475 1103 28-29 0.317722901472969 1103 30-31 0.32912956747122024 1103 32-33 0.3241163406209324 1103 34-35 0.3605062699194548 1103 36-37 0.3029030043510019 1103 38-39 0.28910595741852774 1103 40-41 0.3056066498372658 1103 42-43 0.351643141762608 1103 44-45 0.3660329647303584 1103 46-47 0.3290246729069892 1103 48-49 0.3299624451702243 1103 50-51 0.311085568777699 1103 52-53 0.36248344911983565 1103 54-55 0.3248457770344402 1103 56-57 0.3437072579505269 1103 58-59 0.3136309639584667 1103 60-61 0.2688992822014882 1103 62-63 0.30159886310428874 1103 64-65 0.31365417520395056 1103 66-67 0.26491752767743293 1103 68-69 0.26327741903358515 1103 70-71 0.2685995443634397 1103 72-73 0.3257572418572323 1103 74-75 0.2847038005831024 1103 76-77 0.3221230360813081 1103 78-79 0.3094480131648538 1103 80-81 0.3251753132265165 1103 82-83 0.26941599061728283 1103 84-85 0.26456364410849886 1103 86-87 0.247409706172256 1103 88-89 0.24355800910487346 1103 90-91 0.23078917165292978 1103 92-93 0.23439707027302603 1103 94-95 0.23463874534065 1103 96-97 0.2940189700735125 1103 98-99 0.3697533473614705 1103 100 0.3543353451024913 1104 1 0.17939570683633121 1104 2 0.15448682946279746 1104 3 0.28894013387918704 1104 4 0.19168185800834436 1104 5 0.16740476404022075 1104 6 0.15884989211520661 1104 7 0.1439107156318613 1104 8 0.14618909818652526 1104 9 0.16837767544048177 1104 10-11 0.21269493706335396 1104 12-13 0.12786084999761016 1104 14-15 0.19971634407109207 1104 16-17 0.21945030828879908 1104 18-19 0.22842406832030093 1104 20-21 0.22893216766144064 1104 22-23 0.2093308193577883 1104 24-25 0.24302571374743565 1104 26-27 0.22499044142297464 1104 28-29 0.22070960493115876 1104 30-31 0.24608343985881476 1104 32-33 0.23541339837853315 1104 34-35 0.22811229010953582 1104 36-37 0.2812653186657528 1104 38-39 0.27822679047770293 1104 40-41 0.2236017521924012 1104 42-43 0.26175439363864683 1104 44-45 0.262612714563339 1104 46-47 0.1982638842845077 1104 48-49 0.20771698570965214 1104 50-51 0.21156751402142504 1104 52-53 0.16967611128643512 1104 54-55 0.18863172822997853 1104 56-57 0.1864192748311737 1104 58-59 0.19361528021514118 1104 60-61 0.1809672316277755 1104 62-63 0.20614587447212784 1104 64-65 0.18293788245792086 1104 66-67 0.18649616520053058 1104 68-69 0.16033983476174996 1104 70-71 0.21428850283407996 1104 72-73 0.18960121768094496 1104 74-75 0.19548858306995243 1104 76-77 0.162901705215603 1104 78-79 0.15168074807895948 1104 80-81 0.17824323097198658 1104 82-83 0.16783769427216555 1104 84-85 0.16156694552776685 1104 86-87 0.15733207077246192 1104 88-89 0.1444585692982372 1104 90-91 0.15478143457164606 1104 92-93 0.18686584813517015 1104 94-95 0.1975731441241031 1104 96-97 0.21203450829397852 1104 98-99 0.21091968520914506 1104 100 0.18346707148749175 1105 1 0.15823703317956728 1105 2 0.11333852795217325 1105 3 0.23124795657602704 1105 4 0.16242887450287924 1105 5 0.123633520310932 1105 6 0.061524452913332084 1105 7 0.09529630520966492 1105 8 0.09984713619620322 1105 9 0.11405039185309107 1105 10-11 0.09092585049830859 1105 12-13 0.1279541993129243 1105 14-15 0.1816540688177355 1105 16-17 0.19435562714149057 1105 18-19 0.1443853079733728 1105 20-21 0.14105598566054312 1105 22-23 0.122813378412431 1105 24-25 0.19539331753951927 1105 26-27 0.1594415046688482 1105 28-29 0.21503210603092526 1105 30-31 0.1931559621468253 1105 32-33 0.22291659679324738 1105 34-35 0.24156389415990986 1105 36-37 0.22539699447362693 1105 38-39 0.2354956974472202 1105 40-41 0.27152369069404614 1105 42-43 0.2856299843868513 1105 44-45 0.2898937089171838 1105 46-47 0.3430122992026412 1105 48-49 0.3478672607531337 1105 50-51 0.31325052589893687 1105 52-53 0.28013930631104245 1105 54-55 0.30517199193270983 1105 56-57 0.31179794897224156 1105 58-59 0.35025307034263875 1105 60-61 0.34669683341000734 1105 62-63 0.3460514556553562 1105 64-65 0.337172361570353 1105 66-67 0.3107105356677593 1105 68-69 0.3348600927335781 1105 70-71 0.32093413260899695 1105 72-73 0.3669784873446389 1105 74-75 0.37136994307220306 1105 76-77 0.34357370526403486 1105 78-79 0.2887162716705447 1105 80-81 0.3223784886007621 1105 82-83 0.30602797674162474 1105 84-85 0.31911093634215604 1105 86-87 0.2808162946171038 1105 88-89 0.2889018332552382 1105 90-91 0.3317147602368564 1105 92-93 0.3409408302765158 1105 94-95 0.32468674132161013 1105 96-97 0.3356996494076263 1105 98-99 0.36983677463157605 1105 100 0.3543685603142279 1106 1 0.13947043424118277 1106 2 0.13669308088535104 1106 3 0.24370363738122336 1106 4 0.14398083137811568 1106 5 0.11374881391294167 1106 6 0.13168535486082078 1106 7 0.08739036108010367 1106 8 0.08715267530033799 1106 9 0.12516874002709244 1106 10-11 0.1261796177959411 1106 12-13 0.12315393990408552 1106 14-15 0.19280915915363295 1106 16-17 0.19184248671417947 1106 18-19 0.19849696788206472 1106 20-21 0.2236155648198661 1106 22-23 0.1995722191568774 1106 24-25 0.1814464872089374 1106 26-27 0.2058411205887012 1106 28-29 0.21954692739505077 1106 30-31 0.28181860880480514 1106 32-33 0.2305280370109699 1106 34-35 0.2412210129054273 1106 36-37 0.2614265772577298 1106 38-39 0.2158756663043988 1106 40-41 0.22854026544376183 1106 42-43 0.25108227033744157 1106 44-45 0.24610264972222495 1106 46-47 0.22871864865461333 1106 48-49 0.198078913581071 1106 50-51 0.21308709410560311 1106 52-53 0.26465394557627064 1106 54-55 0.2369030817452895 1106 56-57 0.17908571936759188 1106 58-59 0.20245069514796654 1106 60-61 0.23292686368844073 1106 62-63 0.22815522685287704 1106 64-65 0.20245902741484656 1106 66-67 0.20749648843490576 1106 68-69 0.2519940227885371 1106 70-71 0.31266333617413267 1106 72-73 0.3158487454858232 1106 74-75 0.30526688612433617 1106 76-77 0.25101472867225993 1106 78-79 0.2532154888016507 1106 80-81 0.22559390939063917 1106 82-83 0.31290299110242614 1106 84-85 0.2954902787166631 1106 86-87 0.23619156641726846 1106 88-89 0.2351681359898592 1106 90-91 0.24530558725182772 1106 92-93 0.24624329573907744 1106 94-95 0.2477335510507821 1106 96-97 0.28366155260199477 1106 98-99 0.2651066760465213 1106 100 0.27930193009860105 1107 1 0.088836483933175 1107 2 0.09118003104740069 1107 3 0.16567919536652198 1107 4 0.06199210182622039 1107 5 0.08659748791750843 1107 6 0.06595817703757945 1107 7 0.05765694568388113 1107 8 0.05045383274870119 1107 9 0.10925066833101482 1107 10-11 0.06997466474062719 1107 12-13 0.0831834157094562 1107 14-15 0.12071482131744915 1107 16-17 0.06652044557301906 1107 18-19 0.11136591804244489 1107 20-21 0.14257303616290073 1107 22-23 0.11603180215442421 1107 24-25 0.11958936307676282 1107 26-27 0.16234075158799754 1107 28-29 0.15262812677666915 1107 30-31 0.17364427613226496 1107 32-33 0.15571528970553317 1107 34-35 0.10491265572800046 1107 36-37 0.088660631815209 1107 38-39 0.1049983262131633 1107 40-41 0.10355902447374632 1107 42-43 0.09871163440116959 1107 44-45 0.11311039694411562 1107 46-47 0.13489647121246406 1107 48-49 0.14075835614852394 1107 50-51 0.12716417538898384 1107 52-53 0.1704028567895861 1107 54-55 0.1566459432238787 1107 56-57 0.12905431825821267 1107 58-59 0.14582161825857298 1107 60-61 0.17681291147916056 1107 62-63 0.1782636839920002 1107 64-65 0.13682633139642775 1107 66-67 0.1497590933395614 1107 68-69 0.17891730115972138 1107 70-71 0.16554826136041356 1107 72-73 0.1950938176498127 1107 74-75 0.19805473814654562 1107 76-77 0.1988877522220065 1107 78-79 0.19738512505996653 1107 80-81 0.21935808166136184 1107 82-83 0.23414873985036166 1107 84-85 0.19342078481717806 1107 86-87 0.2047461868760294 1107 88-89 0.179255144776981 1107 90-91 0.18038108037875134 1107 92-93 0.21229606051300465 1107 94-95 0.21234865123259183 1107 96-97 0.1913571685623232 1107 98-99 0.24095247464560643 1107 100 0.23377871395360472 1108 1 0.08087603809927302 1108 2 0.08441436569467697 1108 3 0.15775310834433753 1108 4 0.07641615752525155 1108 5 0.07489268674553529 1108 6 0.057680119858474654 1108 7 0.04158842439652233 1108 8 0.0432732941837628 1108 9 0.08941287212785198 1108 10-11 0.06811419172174027 1108 12-13 0.06928252011493186 1108 14-15 0.1039744531118032 1108 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-0.22188623034039523 2215 6 -0.1769572171851692 2215 7 -0.22578775566121578 2215 8 -0.2524360343638037 2215 9 -0.38441006123774457 2215 10-11 -0.3640560138578479 2215 12-13 -0.36421024914324107 2215 14-15 -0.47738730579176547 2215 16-17 -0.49198834551275894 2215 18-19 -0.503426603845206 2215 20-21 -0.45048092425604835 2215 22-23 -0.42841507766435427 2215 24-25 -0.41630647370229923 2215 26-27 -0.4150133460423575 2215 28-29 -0.4100837331628071 2215 30-31 -0.4259518624742924 2215 32-33 -0.4054996963883184 2215 34-35 -0.4354900365514638 2215 36-37 -0.4824449595244573 2215 38-39 -0.48228080878216417 2215 40-41 -0.4638029091078195 2215 42-43 -0.36638773625826104 2215 44-45 -0.4437271604766053 2215 46-47 -0.3906421387936021 2215 48-49 -0.40337813095874964 2215 50-51 -0.39866642771737304 2215 52-53 -0.42183231253619624 2215 54-55 -0.36040166590444755 2215 56-57 -0.39122773603017436 2215 58-59 -0.34576371574708986 2215 60-61 -0.3833847238484651 2215 62-63 -0.37098522574709136 2215 64-65 -0.38384852773070577 2215 66-67 -0.3412887067686725 2215 68-69 -0.3272346575359748 2215 70-71 -0.30003260496446416 2215 72-73 -0.25983642700659004 2215 74-75 -0.2983971503284337 2215 76-77 -0.3112920928975953 2215 78-79 -0.3133435438644838 2215 80-81 -0.3803352508768967 2215 82-83 -0.40568586275395546 2215 84-85 -0.35609260254521047 2215 86-87 -0.33524464622277605 2215 88-89 -0.38576916520570137 2215 90-91 -0.36179078656197206 2215 92-93 -0.30173479301608097 2215 94-95 -0.33053709040503776 2215 96-97 -0.3065516887620525 2215 98-99 -0.3007197656259173 2215 100 -0.3128847314380643 2216 1 -0.278583832636766 2216 2 -0.290002362038976 2216 3 -0.24685248693226924 2216 4 -0.19087301832347237 2216 5 -0.270202632772353 2216 6 -0.2245555591074151 2216 7 -0.34805409715297486 2216 8 -0.2522677483342619 2216 9 -0.3671772792522603 2216 10-11 -0.32430929299171396 2216 12-13 -0.32897501313081534 2216 14-15 -0.5497843245801448 2216 16-17 -0.5127872522005248 2216 18-19 -0.4607381528705119 2216 20-21 -0.4534272971764608 2216 22-23 -0.43717479406907955 2216 24-25 -0.4441213197746876 2216 26-27 -0.46941725758149744 2216 28-29 -0.5095502261364544 2216 30-31 -0.5544994391676923 2216 32-33 -0.5635683745245998 2216 34-35 -0.5481545808122235 2216 36-37 -0.6189967340634084 2216 38-39 -0.5658289100790626 2216 40-41 -0.6060267882936543 2216 42-43 -0.6801495530828063 2216 44-45 -0.7709706217121166 2216 46-47 -0.7304035062878995 2216 48-49 -0.6869993147129492 2216 50-51 -0.6728664631632029 2216 52-53 -0.60606101678259 2216 54-55 -0.592598480970743 2216 56-57 -0.5793089149251358 2216 58-59 -0.6072435670639962 2216 60-61 -0.602086315869137 2216 62-63 -0.6154746410509375 2216 64-65 -0.6963583875904931 2216 66-67 -0.5768039092279977 2216 68-69 -0.628257780138199 2216 70-71 -0.6245864667041872 2216 72-73 -0.6340403180899585 2216 74-75 -0.6259371840132388 2216 76-77 -0.5139357251314323 2216 78-79 -0.5499920425087765 2216 80-81 -0.6161887254765546 2216 82-83 -0.5961852812674593 2216 84-85 -0.7139424995476844 2216 86-87 -0.6001848062488122 2216 88-89 -0.7410476954141672 2216 90-91 -0.5981746669462282 2216 92-93 -0.630914155131304 2216 94-95 -0.6305392616167325 2216 96-97 -0.598347611750949 2216 98-99 -0.7001034502097347 2216 100 -0.7110643401947172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 477.0 3 44.0 4 96.0 5 167.0 6 447.0 7 1235.0 8 2922.0 9 4740.0 10 6037.0 11 7797.0 12 11663.0 13 15680.0 14 16146.0 15 19608.0 16 25172.0 17 35390.0 18 35007.0 19 22244.0 20 18676.0 21 19377.0 22 21283.0 23 23693.0 24 27959.0 25 35246.0 26 45479.0 27 59507.0 28 78825.0 29 102934.0 30 133767.0 31 173207.0 32 224201.0 33 291989.0 34 402945.0 35 596138.0 36 1016746.0 37 1867307.0 38 2231107.0 39 684472.0 40 1475.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.607089911894086 18.859172908234264 13.414369206368246 24.1193679735034 2 16.606524108771055 19.934198461362477 37.854598693532 25.604678736334467 3 18.248814791546753 24.888560446085044 29.788971463606096 27.073653298762107 4 13.352424174490787 16.973784934980422 36.53624822725598 33.13754266327281 5 13.788984109701543 36.23276410277455 34.190986470590154 15.787265316933741 6 31.56991210109433 36.263591405359634 17.699461155438165 14.467035338107864 7 28.07885774532892 31.36076395634777 21.75373931526938 18.806638983053926 8 26.435364375250796 34.087889221837756 20.07023197007213 19.406514432839316 9 25.58101390777738 16.359526243447537 20.70840755071445 37.35105229806063 10-11 24.849238095411017 25.82882279280081 27.885217713396532 21.43672139839164 12-13 25.506334239980184 23.302549228478515 28.088213627256884 23.102902904284413 14-15 22.84433306436521 25.144446292440254 25.62576244958058 26.385458193613953 16-17 22.52008635544088 28.21481248800871 26.030688017062886 23.23441313948752 18-19 22.468371438352086 26.981787400480606 28.305401972884344 22.244439188282968 20-21 23.379728723479264 26.274918969111212 28.0811998109476 22.264152496461925 22-23 23.06032836614029 26.20063295850908 28.062921813464257 22.67611686188637 24-25 22.849020899256438 26.281359990790687 28.090345921774347 22.779273188178525 26-27 22.77856038512553 26.252570373380873 28.09369459228242 22.87517464921118 28-29 22.808376146436398 26.228891150465778 28.222645687867892 22.740087015229935 30-31 22.973482682005834 26.53088744317566 27.906794468845646 22.58883540597286 32-33 22.714917047755417 26.314709281304083 28.154641959357228 22.815731711583272 34-35 22.834331755314572 26.03363577919584 28.194568030830375 22.937464434659212 36-37 23.158349557764218 26.27406973608205 27.85460206526797 22.71297864088576 38-39 23.010214077343395 26.363911087213577 27.87403132221828 22.75184351322475 40-41 22.905366224360034 26.203676596842868 28.01686970518022 22.874087473616875 42-43 22.821114320159342 26.322867911455123 27.91226977664657 22.94374799173896 44-45 22.920089798114027 26.442914211716023 27.750726952676843 22.886269037493108 46-47 23.080131307752634 26.503434185069658 27.410553886643385 23.00588062053432 48-49 23.058826391842377 26.42421668135484 27.431548380821795 23.085408545980986 50-51 23.035205322620435 26.45840298629956 27.332435238780256 23.173956452299752 52-53 23.037320840699856 26.52224313729193 27.23390224057955 23.206533781428664 54-55 23.09181196496463 26.80731581855384 27.180221940059635 22.920650276421892 56-57 22.842007717651867 26.617153227836443 27.18882015990066 23.352018894611028 58-59 22.89032982789128 26.262205774522535 27.389432712731594 23.458031684854596 60-61 23.073080276565047 26.538144626151905 27.246255781391643 23.142519315891402 62-63 22.845868782522977 26.608352549215585 27.297194946217417 23.248583722044017 64-65 22.875943144309854 26.348333824787595 27.403078198362564 23.372644832539986 66-67 22.736153824365143 26.436159949411476 27.304457895874695 23.523228330348683 68-69 23.089730002056008 26.50117771055151 27.150469447040432 23.258622840352057 70-71 23.06273300583021 26.297804306363236 27.23335314685463 23.40610954095192 72-73 22.841185080671323 26.456844642941878 27.28841511184364 23.41355516454316 74-75 22.787065300105365 26.464902353158422 27.216563119029036 23.531469227707177 76-77 22.84838347626684 26.47435888124511 27.02179251787637 23.655465124611677 78-79 22.88645164328268 26.572303947603114 27.146416249697452 23.394828159416754 80-81 22.74349000842381 26.43131811963745 27.206165661293173 23.61902621064557 82-83 22.725936789822416 26.425239476691953 27.21570563280749 23.63311810067814 84-85 22.76000390747137 26.443376708928607 27.04424727127644 23.75237211232358 86-87 22.818848043068183 26.484446716664245 27.031435819309785 23.66526942095779 88-89 22.623182598948567 26.392355264607296 27.149953214519602 23.834508921924535 90-91 22.513590980265246 26.347015776558663 27.12957637346811 24.009816869707983 92-93 22.613500403577437 26.515158484640573 27.02149443247135 23.84984667931064 94-95 22.583067130068855 26.241985833683017 27.152073256330034 24.022873779918097 96-97 22.463371283609284 26.32736086462376 27.174345269676227 24.03492258209073 98-99 22.35222719529106 26.356977680141565 27.184106042675076 24.1066890818923 100 22.47631505235225 26.20024998928721 27.10972341152338 24.21371154683716 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1118.0 1 833.5 2 3038.0 3 4511.5 4 2305.5 5 1072.5 6 1507.5 7 3185.0 8 5489.5 9 6598.0 10 5199.5 11 3500.5 12 3302.5 13 3510.5 14 3945.0 15 4467.0 16 4942.5 17 5619.0 18 6314.0 19 7070.5 20 8095.0 21 9468.0 22 11610.0 23 15001.5 24 19801.5 25 26145.0 26 34769.5 27 46195.0 28 60498.5 29 76761.0 30 92539.5 31 107970.5 32 123296.0 33 137807.5 34 152791.5 35 169420.5 36 186707.5 37 204926.5 38 222854.0 39 236528.0 40 249075.0 41 261568.5 42 271104.5 43 277487.5 44 281413.5 45 284908.0 46 287963.5 47 286852.0 48 283174.5 49 284271.5 50 287166.0 51 285411.0 52 277570.0 53 272419.5 54 269047.5 55 258115.5 56 243496.0 57 224432.0 58 206272.0 59 192842.0 60 174946.5 61 152014.5 62 130822.0 63 110428.5 64 89923.0 65 72567.5 66 56643.5 67 43094.5 68 33020.5 69 24941.0 70 18408.5 71 13760.0 72 10645.0 73 7803.0 74 5792.5 75 4176.5 76 2689.5 77 1807.5 78 1254.5 79 797.0 80 422.5 81 192.5 82 102.0 83 57.5 84 29.0 85 21.0 86 26.5 87 25.0 88 17.5 89 12.5 90 7.5 91 3.0 92 2.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09768550713848646 2 0.0 3 0.0 4 1.210477164045681E-5 5 0.009151207360185348 6 1.210477164045681E-4 7 0.0 8 1.210477164045681E-4 9 0.0 10-11 0.0 12-13 0.013291039261221577 14-15 3.6314314921370426E-5 16-17 0.0 18-19 1.210477164045681E-5 20-21 4.2366700741598835E-5 22-23 0.0 24-25 2.420954328091362E-5 26-27 3.0261929101142024E-5 28-29 9.078578730342607E-5 30-31 0.0 32-33 7.262862984274085E-5 34-35 2.420954328091362E-5 36-37 1.210477164045681E-5 38-39 1.0289055894388288E-4 40-41 6.657624402251246E-5 42-43 1.8762396042708055E-4 44-45 8.473340148319767E-5 46-47 2.420954328091362E-5 48-49 2.84462133550735E-4 50-51 1.3315248804502493E-4 52-53 1.9972873206753738E-4 54-55 1.271001022247965E-4 56-57 1.6341441714616694E-4 58-59 3.0261929101142024E-5 60-61 2.420954328091362E-5 62-63 3.207764484721055E-4 64-65 1.0894294476411129E-4 66-67 5.205051805396428E-4 68-69 5.991861962026121E-4 70-71 1.3920487386525333E-4 72-73 3.510383775732475E-4 74-75 3.8130030667438955E-4 76-77 2.54200204449593E-4 78-79 0.0012528438647872798 80-81 0.0011620580774838536 82-83 8.110196999106063E-4 84-85 0.0013073153371693355 86-87 8.654911722926619E-4 88-89 0.001149953305843397 90-91 0.0014465202110345889 92-93 0.001077324676000656 94-95 0.0015796726990796137 96-97 0.001597829856540299 98-99 0.0018580824468101204 100 6.173433536632973E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8261205.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.977564475861406 #Duplication Level Percentage of deduplicated Percentage of total 1 73.16795357800811 38.76259978245759 2 15.008641747428896 15.902425717390392 3 4.86063719776598 7.725141616152525 4 2.196722676920855 4.655080690086459 5 1.2264916535073667 3.2488270326396194 6 0.7671893581979939 2.4386294213477377 7 0.5286159921189895 1.9603351463818648 8 0.3809641574812699 1.6146042572764956 9 0.26618504790394426 1.2691651984057317 >10 1.457526750380456 13.850040061687269 >50 0.09539169253282545 3.46324940488797 >100 0.041936306593033656 3.8071130351193463 >500 0.001330972125107014 0.4569312677218035 >1k 3.211203764480294E-4 0.2946873087970591 >5k 4.587432935902645E-5 0.1898679876097442 >10k+ 4.587432935902645E-5 0.3613020720384335 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15330 0.1855661492482029 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14403 0.17434502593749943 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.210477164045681E-5 0.0 0.0 0.0 4 0.0 1.210477164045681E-5 0.0 2.420954328091362E-5 0.0 5 0.0 3.631431492137043E-5 0.0 2.420954328091362E-5 0.0 6 0.0 3.631431492137043E-5 0.0 2.420954328091362E-5 0.0 7 0.0 3.631431492137043E-5 0.0 2.420954328091362E-5 0.0 8 0.0 4.841908656182724E-5 0.0 2.420954328091362E-5 0.0 9 0.0 4.841908656182724E-5 0.0 3.631431492137043E-5 0.0 10-11 0.0 4.841908656182724E-5 0.0 4.2366700741598835E-5 0.0 12-13 0.0 4.841908656182724E-5 0.0 4.841908656182724E-5 0.0 14-15 0.0 8.473340148319767E-5 0.0 7.262862984274087E-5 0.0 16-17 0.0 1.0894294476411129E-4 0.0 8.473340148319767E-5 0.0 18-19 0.0 1.149953305843397E-4 0.0 1.0289055894388289E-4 0.0 20-21 0.0 1.210477164045681E-4 0.0 1.331524880450249E-4 0.0 22-23 0.0 1.331524880450249E-4 0.0 2.420954328091362E-4 0.0 24-25 0.0 1.573620313259385E-4 1.210477164045681E-5 6.536576685846678E-4 0.0 26-27 0.0 1.8157157460685214E-4 1.210477164045681E-5 0.0011620580774838536 0.0 28-29 0.0 1.8762396042708055E-4 1.210477164045681E-5 0.0026691021467207267 0.0 30-31 0.0 1.9972873206753738E-4 1.210477164045681E-5 0.007541272732004593 0.0 32-33 0.0 2.299906611686794E-4 1.210477164045681E-5 0.01829030994873024 0.0 34-35 0.0 2.54200204449593E-4 1.210477164045681E-5 0.03421413704175117 0.0 36-37 0.0 2.84462133550735E-4 1.210477164045681E-5 0.06031807708439628 0.0 38-39 0.0 3.1472406265187707E-4 1.210477164045681E-5 0.09809101698844175 0.0 40-41 0.0 3.207764484721055E-4 1.210477164045681E-5 0.14226738109028889 0.0 42-43 0.0 3.510383775732475E-4 1.210477164045681E-5 0.18834419433968774 0.0 44-45 0.0 3.510383775732475E-4 1.210477164045681E-5 0.2426098856038556 0.0 46-47 0.0 3.752479208541611E-4 1.210477164045681E-5 0.311782603143246 0.0 48-49 0.0 4.0550984995530316E-4 1.210477164045681E-5 0.38592432944104405 0.0 50-51 0.0 4.4787655069690197E-4 1.210477164045681E-5 0.4641453637816759 0.0 52-53 0.0 4.4787655069690197E-4 1.210477164045681E-5 0.5439400184355672 0.0 54-55 1.210477164045681E-5 4.4787655069690197E-4 1.210477164045681E-5 0.633551642889869 0.0 56-57 1.210477164045681E-5 4.4787655069690197E-4 1.210477164045681E-5 0.7378039886433032 0.0 58-59 1.210477164045681E-5 4.599813223373588E-4 1.210477164045681E-5 0.8540763726357111 0.0 60-61 1.210477164045681E-5 4.720860939778156E-4 1.210477164045681E-5 0.9757959038663246 0.0 62-63 1.210477164045681E-5 4.720860939778156E-4 1.210477164045681E-5 1.1023997104538625 0.0 64-65 1.210477164045681E-5 4.78138479798044E-4 2.420954328091362E-5 1.2398736019745304 0.0 66-67 1.210477164045681E-5 5.08400408899186E-4 2.420954328091362E-5 1.396388299285637 0.0 68-69 1.210477164045681E-5 5.205051805396428E-4 2.420954328091362E-5 1.568046065918955 0.0 70-71 1.210477164045681E-5 5.205051805396428E-4 2.420954328091362E-5 1.7493089688489754 0.0 72-73 1.210477164045681E-5 5.205051805396428E-4 2.420954328091362E-5 1.945091545361724 0.0 74-75 1.210477164045681E-5 5.38662338000328E-4 2.420954328091362E-5 2.1426474709197993 0.0 76-77 1.210477164045681E-5 5.568194954610132E-4 2.420954328091362E-5 2.35518910376876 0.0 78-79 1.210477164045681E-5 5.6892426710147E-4 2.420954328091362E-5 2.59129872700169 0.0 80-81 1.210477164045681E-5 5.6892426710147E-4 2.420954328091362E-5 2.8464007369384974 0.0 82-83 1.210477164045681E-5 5.6892426710147E-4 2.420954328091362E-5 3.116379511221426 0.0 84-85 1.210477164045681E-5 5.810290387419268E-4 2.420954328091362E-5 3.3840341693493867 0.0 86-87 1.210477164045681E-5 5.991861962026121E-4 2.420954328091362E-5 3.666783477712997 0.0 88 1.210477164045681E-5 6.052385820228405E-4 2.420954328091362E-5 3.896501781519766 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11740 0.0 49.052593 1 TATAACG 1080 0.0 40.039562 2 GTATCAA 19365 0.0 36.200737 1 TAACGCA 1320 0.0 33.827892 4 ATAACGC 1365 0.0 32.023994 3 TACCTGG 7535 0.0 30.316442 2 ATCAACG 22550 0.0 30.307013 3 TCAACGC 22700 0.0 30.168863 4 GTACCTG 8110 0.0 29.760843 1 CAACGCA 23690 0.0 28.987473 5 TATCAAC 24245 0.0 28.323914 2 AACGCAG 25085 0.0 27.71592 6 ACGCAGA 28735 0.0 24.228083 7 TATCACG 1305 0.0 23.771612 2 CGCAGAG 29540 0.0 23.583752 8 ACCTGGG 9660 0.0 23.112225 3 GTACATG 24740 0.0 22.535507 1 GTATAAC 2780 0.0 22.170485 1 TACATGG 25670 0.0 20.983793 2 GCAGAGT 33070 0.0 20.881554 9 >>END_MODULE