FastQCFastQC Report
Wed 25 May 2016
SRR2049483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7255682
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8500.033.7315522
GTATCAA104550.033.3281481
GGTATCA65050.032.819021
TACCTGG66600.031.4764732
TCAACGC107800.030.5201534
ATCAACG109200.030.0438233
GTACCTG74100.029.5722771
TAACGCA11500.028.2006234
CAACGCA117650.028.0448175
AACGCAG128100.026.1611526
TATCAAC128300.026.0476382
ATAACGC11850.024.9887563
GTACATG219750.024.4373591
ACCTGGG86850.023.6501583
ACGCAGA145550.022.9601027
TACATGG226800.022.423822
ACATGGG223200.022.1535823
CGCAGAG150800.022.0038598
TATCACG11000.019.228492
GTATAAC23600.019.1282981
Produced by FastQC (version 0.11.5)