FastQCFastQC Report
Wed 25 May 2016
SRR2049483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7255682
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203240.2801115043354987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185350.2554549661906351No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151120.20827814669937297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109550.1509851175947347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113600.051.756911
TATAACG8550.038.4801062
GTATCAA195150.038.010171
TATCACG12500.033.4644972
GTACCTG71000.032.6606831
TACCTGG68950.032.515352
ATAACGC10650.032.2164463
ATCAACG233350.031.4412233
TAACGCA10850.031.1894054
TCAACGC235000.031.1804644
CAACGCA244750.030.0151545
TATCAAC250700.029.377782
AACGCAG258100.028.705916
ACCTGGG86850.025.5973873
ACGCAGA294450.025.1621517
CGCAGAG301450.024.5778588
GCAGAGT334450.021.9840979
GTACATG218750.021.7175061
TCACGCA19750.020.9420854
AGAGTAC312900.020.71866810-11
Produced by FastQC (version 0.11.5)