##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049483_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7255682 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.675478886753858 31.0 31.0 34.0 30.0 34.0 2 31.954641617424798 33.0 31.0 34.0 30.0 34.0 3 31.944823519002075 33.0 31.0 34.0 30.0 34.0 4 35.67105669735802 37.0 35.0 37.0 33.0 37.0 5 35.75308082686094 37.0 35.0 37.0 35.0 37.0 6 35.8168394094449 37.0 35.0 37.0 35.0 37.0 7 35.78822294031078 37.0 35.0 37.0 33.0 37.0 8 35.782040061843944 37.0 35.0 37.0 33.0 37.0 9 37.492242907007224 39.0 37.0 39.0 35.0 39.0 10-11 37.45062324947537 39.0 37.0 39.0 34.5 39.0 12-13 37.42212647963348 39.0 37.0 39.0 34.0 39.0 14-15 38.69981484855593 40.0 38.0 41.0 34.0 41.0 16-17 38.69993268723739 40.0 38.0 41.0 34.0 41.0 18-19 38.75467330293692 40.0 38.0 41.0 34.0 41.0 20-21 38.758960081216344 40.0 38.0 41.0 34.0 41.0 22-23 38.7180017674424 40.0 38.0 41.0 34.0 41.0 24-25 38.689089667931974 40.0 38.0 41.0 34.0 41.0 26-27 38.58163016240238 40.0 38.0 41.0 34.0 41.0 28-29 38.496989393967375 40.0 38.0 41.0 34.0 41.0 30-31 38.398421678348086 40.0 38.0 41.0 34.0 41.0 32-33 38.274069274259816 40.0 38.0 41.0 34.0 41.0 34-35 38.162223551142404 40.0 38.0 41.0 34.0 41.0 36-37 38.04781507789343 40.0 38.0 41.0 33.0 41.0 38-39 37.96041729778124 40.0 37.0 41.0 33.0 41.0 40-41 37.87935358798801 40.0 37.0 41.0 33.0 41.0 42-43 37.748200651572105 40.0 37.0 41.0 33.0 41.0 44-45 37.58503066975648 40.0 37.0 41.0 33.0 41.0 46-47 37.36914083334965 40.0 37.0 41.0 32.5 41.0 48-49 37.15138101421755 40.0 36.5 41.0 32.0 41.0 50-51 36.98813350695359 40.0 36.0 41.0 31.0 41.0 52-53 36.849143953662804 39.5 36.0 41.0 31.0 41.0 54-55 36.68440037752481 39.0 35.0 41.0 31.0 41.0 56-57 36.50834442578933 39.0 35.0 41.0 31.0 41.0 58-59 36.29687533163664 39.0 35.0 41.0 31.0 41.0 60-61 36.07465004668066 38.5 35.0 40.5 30.0 41.0 62-63 35.82352148288748 38.0 35.0 40.0 30.0 41.0 64-65 35.57048786041064 37.5 35.0 40.0 30.0 41.0 66-67 35.27758190064007 37.0 34.5 40.0 29.0 41.0 68-69 34.96059343559985 36.5 34.0 39.0 29.0 41.0 70-71 34.645610226578285 36.0 34.0 39.0 29.0 41.0 72-73 34.316846369507374 35.5 34.0 39.0 29.0 40.5 74-75 34.00093251054828 35.0 34.0 37.5 29.0 40.0 76-77 33.694888364732634 35.0 34.0 37.0 29.0 39.0 78-79 33.40059170178627 35.0 34.0 37.0 28.0 39.0 80-81 33.120147148124744 35.0 33.5 36.0 28.0 39.0 82-83 32.870649995410496 35.0 33.0 36.0 28.0 37.5 84-85 32.62657197490188 35.0 33.0 35.5 27.0 37.0 86-87 32.41571433257411 35.0 33.0 35.0 27.0 37.0 88-89 32.22270229042563 35.0 33.0 35.0 27.0 36.0 90-91 32.049544756785096 35.0 33.0 35.0 26.5 36.0 92-93 31.895387780225207 35.0 33.0 35.0 26.0 36.0 94-95 31.739881378483787 35.0 33.0 35.0 26.0 35.5 96-97 31.57834184298595 35.0 33.0 35.0 25.0 35.0 98-99 31.43182350053379 35.0 33.0 35.0 25.0 35.0 100 31.31341175095601 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2848891288658315 1101 2 0.32034110381391656 1101 3 0.4651719377829586 1101 4 0.30779984886110157 1101 5 0.28535980897594015 1101 6 0.2791063958709685 1101 7 0.25923240638064726 1101 8 0.24412360158539315 1101 9 0.3747594142607866 1101 10-11 0.3380728967837143 1101 12-13 0.2503805708732827 1101 14-15 0.4210572765745084 1101 16-17 0.39748957610395763 1101 18-19 0.42080598431417116 1101 20-21 0.42341886106304116 1101 22-23 0.42977192015766974 1101 24-25 0.3855569228443585 1101 26-27 0.44170319581514406 1101 28-29 0.44718529576145727 1101 30-31 0.4501103426653472 1101 32-33 0.46058300825023224 1101 34-35 0.4427286004211126 1101 36-37 0.4807215228338748 1101 38-39 0.455792435949931 1101 40-41 0.4197473791684061 1101 42-43 0.4404815807478286 1101 44-45 0.47953820093083976 1101 46-47 0.4447337846847148 1101 48-49 0.4983199154777438 1101 50-51 0.40785443515639486 1101 52-53 0.4117173796546183 1101 54-55 0.38412957429930117 1101 56-57 0.4585422408375166 1101 58-59 0.41680534190825824 1101 60-61 0.427114360768563 1101 62-63 0.42226660323064635 1101 64-65 0.40330645570232093 1101 66-67 0.4282140323566068 1101 68-69 0.37474755669766324 1101 70-71 0.37226143806613976 1101 72-73 0.3851377723528273 1101 74-75 0.39911894516723834 1101 76-77 0.33858950252227515 1101 78-79 0.34454074434334814 1101 80-81 0.3745541789773199 1101 82-83 0.3377355716507964 1101 84-85 0.3472578472593497 1101 86-87 0.34812914213942037 1101 88-89 0.394266461461541 1101 90-91 0.3431382535812446 1101 92-93 0.3378544519081359 1101 94-95 0.3466717348546773 1101 96-97 0.3931072606175654 1101 98-99 0.4548651843739222 1101 100 0.45306696087352805 1102 1 0.26207062028707995 1102 2 0.2740645385893572 1102 3 0.39432604880974154 1102 4 0.2530332905069699 1102 5 0.24842859613560364 1102 6 0.18470812272219206 1102 7 0.1875284090808904 1102 8 0.21454561351266221 1102 9 0.2362604572577638 1102 10-11 0.27036020825695317 1102 12-13 0.2578004018550999 1102 14-15 0.35257135279479 1102 16-17 0.3143934700422335 1102 18-19 0.3397277784181796 1102 20-21 0.3466981330834784 1102 22-23 0.3316263771470105 1102 24-25 0.33540858373820015 1102 26-27 0.3483036270305817 1102 28-29 0.3554300336964502 1102 30-31 0.35765300471194195 1102 32-33 0.33868877110525375 1102 34-35 0.3951052250807763 1102 36-37 0.3655019147167877 1102 38-39 0.3686514865570416 1102 40-41 0.3587686327044963 1102 42-43 0.36678838124184665 1102 44-45 0.38757810587987507 1102 46-47 0.3769968066607916 1102 48-49 0.3515101523839874 1102 50-51 0.3787160860780716 1102 52-53 0.3918951605070191 1102 54-55 0.3888875304999857 1102 56-57 0.3653627198859013 1102 58-59 0.3762964154502271 1102 60-61 0.4261609347037947 1102 62-63 0.42596375678824216 1102 64-65 0.3618434294760462 1102 66-67 0.3891619602775691 1102 68-69 0.417600170949747 1102 70-71 0.41531150169494424 1102 72-73 0.3567297404509375 1102 74-75 0.3677471695900749 1102 76-77 0.3368360015814602 1102 78-79 0.3699771696817393 1102 80-81 0.34391415389204383 1102 82-83 0.3520987941327789 1102 84-85 0.3163418840815382 1102 86-87 0.3254794437573736 1102 88-89 0.30043368325534914 1102 90-91 0.3395795076554009 1102 92-93 0.3696647361047454 1102 94-95 0.3574450130618878 1102 96-97 0.342003849620685 1102 98-99 0.40461210472386 1102 100 0.4502589557389136 1103 1 0.26277608193129254 1103 2 0.27513058633891774 1103 3 0.3739376941681307 1103 4 0.24766547842118314 1103 5 0.230719020699361 1103 6 0.16505289264380707 1103 7 0.20247354971906617 1103 8 0.19853731199652458 1103 9 0.2333617741288876 1103 10-11 0.22951510300055844 1103 12-13 0.24421620994150572 1103 14-15 0.3365528856179907 1103 16-17 0.35397158632331127 1103 18-19 0.28206604759783005 1103 20-21 0.320397760254572 1103 22-23 0.3387635554764685 1103 24-25 0.31878022532957573 1103 26-27 0.3621842161636195 1103 28-29 0.3775169162095793 1103 30-31 0.39034870028076085 1103 32-33 0.43040372245970815 1103 34-35 0.4082204675867942 1103 36-37 0.40473635245454176 1103 38-39 0.41652104132751333 1103 40-41 0.433085513510143 1103 42-43 0.43736655649255596 1103 44-45 0.4202582185030721 1103 46-47 0.4173385796928315 1103 48-49 0.42636386808698035 1103 50-51 0.4426242413143129 1103 52-53 0.4327082593348379 1103 54-55 0.47015986634876583 1103 56-57 0.39788073202836216 1103 58-59 0.4106607184027524 1103 60-61 0.43586487193898193 1103 62-63 0.4105781349930666 1103 64-65 0.40026272205167146 1103 66-67 0.3432801354226598 1103 68-69 0.4041701714250223 1103 70-71 0.33894407951285643 1103 72-73 0.3618736351575791 1103 74-75 0.3711292408852458 1103 76-77 0.37343518762449435 1103 78-79 0.3466031385000079 1103 80-81 0.332520278988639 1103 82-83 0.3267398714991785 1103 84-85 0.3611215400861454 1103 86-87 0.33719761643806123 1103 88-89 0.3361592737878212 1103 90-91 0.3043733119712755 1103 92-93 0.3594017623453567 1103 94-95 0.30506094119990124 1103 96-97 0.32880560075475174 1103 98-99 0.37667700124535486 1103 100 0.39743372940993993 1104 1 0.2333821476920157 1104 2 0.24163753012626188 1104 3 0.3417769094530989 1104 4 0.228256064394742 1104 5 0.22293148620599368 1104 6 0.20365158333801503 1104 7 0.1913196248216309 1104 8 0.14819858216693405 1104 9 0.22439106262749675 1104 10-11 0.24502726231352057 1104 12-13 0.22728815601798402 1104 14-15 0.3351031562386524 1104 16-17 0.30965911880113595 1104 18-19 0.2903272857630128 1104 20-21 0.30403997267442406 1104 22-23 0.28136191911816155 1104 24-25 0.32608253694262146 1104 26-27 0.32531718589182645 1104 28-29 0.3454163526484706 1104 30-31 0.3692616498630059 1104 32-33 0.32220052926916054 1104 34-35 0.35145374886542413 1104 36-37 0.31207526851908085 1104 38-39 0.35263756113560873 1104 40-41 0.33943070867200476 1104 42-43 0.3335977809013144 1104 44-45 0.3222286507703984 1104 46-47 0.3317901103128449 1104 48-49 0.292767516066867 1104 50-51 0.25846110449948867 1104 52-53 0.2702204980183254 1104 54-55 0.25728350654107146 1104 56-57 0.2909196519210937 1104 58-59 0.33915099800003645 1104 60-61 0.3018943717992073 1104 62-63 0.3131407602586407 1104 64-65 0.26499645256675564 1104 66-67 0.3230277013314762 1104 68-69 0.2620361160082396 1104 70-71 0.2970498172403424 1104 72-73 0.3210053095264911 1104 74-75 0.31934553731284865 1104 76-77 0.26446148831518457 1104 78-79 0.27224826659428203 1104 80-81 0.23440453066737632 1104 82-83 0.27453448880511644 1104 84-85 0.26226664325665183 1104 86-87 0.19719689307403598 1104 88-89 0.1617629631675257 1104 90-91 0.15306893936257637 1104 92-93 0.18564951709309696 1104 94-95 0.21101534140059286 1104 96-97 0.16092738671380502 1104 98-99 0.23946643731698458 1104 100 0.20446031302084577 1105 1 0.22612635720380325 1105 2 0.21090936393473214 1105 3 0.31140861443742907 1105 4 0.22068761527003034 1105 5 0.1718120480120362 1105 6 0.18232046683440473 1105 7 0.18133581286390665 1105 8 0.1682213499366938 1105 9 0.20036686487102884 1105 10-11 0.189012138373009 1105 12-13 0.19337296798396153 1105 14-15 0.22773265380420327 1105 16-17 0.2856799875595115 1105 18-19 0.2674457155049623 1105 20-21 0.24384939084654178 1105 22-23 0.21853659444912665 1105 24-25 0.24367353499154376 1105 26-27 0.19627674395373873 1105 28-29 0.26070653090297924 1105 30-31 0.24767360852995068 1105 32-33 0.3116762822438872 1105 34-35 0.27380529023488265 1105 36-37 0.27206898564471516 1105 38-39 0.2854056525547577 1105 40-41 0.25777508780295477 1105 42-43 0.26904287470902943 1105 44-45 0.31711124315626193 1105 46-47 0.26742922350435805 1105 48-49 0.28998523200026227 1105 50-51 0.2945517575688612 1105 52-53 0.3254205243124275 1105 54-55 0.2855436425412563 1105 56-57 0.20705693051460372 1105 58-59 0.2039655209361655 1105 60-61 0.22754450380664792 1105 62-63 0.2154778405235831 1105 64-65 0.1689410561506577 1105 66-67 0.23880430500990002 1105 68-69 0.2388454978203356 1105 70-71 0.27858146480259904 1105 72-73 0.30963998408652316 1105 74-75 0.28018585295045995 1105 76-77 0.23229515114623922 1105 78-79 0.22725851904282024 1105 80-81 0.2585476981479857 1105 82-83 0.19311544877948705 1105 84-85 0.1935590817074484 1105 86-87 0.1964539606018718 1105 88-89 0.19960463760585156 1105 90-91 0.21624816632179034 1105 92-93 0.24857700232085733 1105 94-95 0.23183179145042487 1105 96-97 0.2885175485475564 1105 98-99 0.2924834385311925 1105 100 0.31936560545022274 1106 1 0.177774418727207 1106 2 0.1853178636486703 1106 3 0.2948665089008351 1106 4 0.16223821506327596 1106 5 0.14749682696231048 1106 6 0.15334604136854324 1106 7 0.14308124026352687 1106 8 0.13887403666831943 1106 9 0.19127023010476307 1106 10-11 0.16069859387259555 1106 12-13 0.17684644670045913 1106 14-15 0.23802663208715558 1106 16-17 0.25863952716409244 1106 18-19 0.24786298719637045 1106 20-21 0.2314335977341102 1106 22-23 0.21793915281634924 1106 24-25 0.17166170488997068 1106 26-27 0.2344105485774648 1106 28-29 0.22288890848839316 1106 30-31 0.25049437431474786 1106 32-33 0.20816037729547077 1106 34-35 0.22152304466590778 1106 36-37 0.24587797487834706 1106 38-39 0.2416200440141978 1106 40-41 0.27385457027347826 1106 42-43 0.25157101790509273 1106 44-45 0.23373129551934824 1106 46-47 0.2635278643018708 1106 48-49 0.24021914437020797 1106 50-51 0.19036888492979642 1106 52-53 0.17694603018319555 1106 54-55 0.2580507275852426 1106 56-57 0.23165581532430224 1106 58-59 0.24235475234668513 1106 60-61 0.21350878709491639 1106 62-63 0.20926533099650158 1106 64-65 0.22377029267594395 1106 66-67 0.18882624437920725 1106 68-69 0.21626053624274988 1106 70-71 0.2823170206522505 1106 72-73 0.30439238824711623 1106 74-75 0.3018279312709282 1106 76-77 0.26690251327839576 1106 78-79 0.2543194123031256 1106 80-81 0.2466477510018521 1106 82-83 0.24981961233596195 1106 84-85 0.25731343834890197 1106 86-87 0.2271157029747286 1106 88-89 0.2234228879276472 1106 90-91 0.22303979340343716 1106 92-93 0.2475505344279938 1106 94-95 0.23412831606134077 1106 96-97 0.23377358208978904 1106 98-99 0.2720842902637415 1106 100 0.27824466455305696 1107 1 0.11700927011484907 1107 2 0.12700100860444508 1107 3 0.2181640806075258 1107 4 0.14052909795892532 1107 5 0.13620896595426046 1107 6 0.13937026737055902 1107 7 0.1041373950307829 1107 8 0.12442203874477542 1107 9 0.1152794790902476 1107 10-11 0.14681660731881863 1107 12-13 0.12554816817168302 1107 14-15 0.16605477466348617 1107 16-17 0.15443775334293264 1107 18-19 0.14552964031548754 1107 20-21 0.1563768761622626 1107 22-23 0.16047785320498775 1107 24-25 0.1730856154454159 1107 26-27 0.21024166163901015 1107 28-29 0.22853295645676042 1107 30-31 0.20818955717265908 1107 32-33 0.19587893634005127 1107 34-35 0.2082143937227059 1107 36-37 0.1829465371688599 1107 38-39 0.20389268689056905 1107 40-41 0.22443021210057879 1107 42-43 0.22678238519549154 1107 44-45 0.2042221312256629 1107 46-47 0.2548271123030048 1107 48-49 0.2484494830128554 1107 50-51 0.20689244479154212 1107 52-53 0.21519398534439205 1107 54-55 0.2349898817621181 1107 56-57 0.24103532326305555 1107 58-59 0.20462080155813567 1107 60-61 0.15046771991729457 1107 62-63 0.23167667733125796 1107 64-65 0.18614727912517282 1107 66-67 0.2173585556475004 1107 68-69 0.21608260909209065 1107 70-71 0.18885419698023753 1107 72-73 0.23681618345258215 1107 74-75 0.21683580177323591 1107 76-77 0.22212407725655936 1107 78-79 0.15330653688024398 1107 80-81 0.20264499074649223 1107 82-83 0.23804618507187314 1107 84-85 0.22297913264576863 1107 86-87 0.1546838121269758 1107 88-89 0.210332031531955 1107 90-91 0.23602416445982044 1107 92-93 0.22634648657552958 1107 94-95 0.23182859138992384 1107 96-97 0.24716850667494228 1107 98-99 0.32812878591219885 1107 100 0.3129541161175382 1108 1 0.08410087882725037 1108 2 0.12726152889488418 1108 3 0.1945096647886011 1108 4 0.11142749216051584 1108 5 0.11418423282380274 1108 6 0.13045383761672724 1108 7 0.08786888150060435 1108 8 0.07511612920730215 1108 9 0.1754625357270143 1108 10-11 0.11289961808187599 1108 12-13 0.11937165393940319 1108 14-15 0.14936866059770892 1108 16-17 0.16264950339285633 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12-13 -0.43338255693040395 2215 14-15 -0.60107806758176 2215 16-17 -0.5851707798652015 2215 18-19 -0.568190276781344 2215 20-21 -0.5928658676001035 2215 22-23 -0.5446098799380579 2215 24-25 -0.5709966471142849 2215 26-27 -0.5271621367337005 2215 28-29 -0.4944922796704887 2215 30-31 -0.5587675712087687 2215 32-33 -0.5302115108737269 2215 34-35 -0.5464936170964378 2215 36-37 -0.5476566770972795 2215 38-39 -0.5816771684886675 2215 40-41 -0.5970451543586464 2215 42-43 -0.6046518833626706 2215 44-45 -0.6276888128589064 2215 46-47 -0.5932211573940123 2215 48-49 -0.5391118035247544 2215 50-51 -0.5022761096552131 2215 52-53 -0.5576550616582452 2215 54-55 -0.5101023652532461 2215 56-57 -0.5001578731244365 2215 58-59 -0.5298346335685409 2215 60-61 -0.5367168174395331 2215 62-63 -0.5224996713925663 2215 64-65 -0.5734724083433278 2215 66-67 -0.5661044244101205 2215 68-69 -0.5253671949678207 2215 70-71 -0.5740613799023677 2215 72-73 -0.5573534718308153 2215 74-75 -0.5356100892324491 2215 76-77 -0.5063646022523116 2215 78-79 -0.514495793091335 2215 80-81 -0.5496477889146476 2215 82-83 -0.5708527638722884 2215 84-85 -0.5287197810185802 2215 86-87 -0.4734919026210491 2215 88-89 -0.4981173649089392 2215 90-91 -0.5626646462652474 2215 92-93 -0.5195127539975068 2215 94-95 -0.5239729282067387 2215 96-97 -0.5099178086435749 2215 98-99 -0.487433916239425 2215 100 -0.46282294633747867 2216 1 -0.5663375959816612 2216 2 -0.612879653551083 2216 3 -0.6029717083192949 2216 4 -0.49148642537639375 2216 5 -0.5308518060503715 2216 6 -0.4526184283222605 2216 7 -0.5263598055088039 2216 8 -0.42617242135619904 2216 9 -0.6418464231840701 2216 10-11 -0.5823855693350595 2216 12-13 -0.6142059955027221 2216 14-15 -0.8194274441839937 2216 16-17 -0.8528442789237616 2216 18-19 -0.8202357335106711 2216 20-21 -0.8089357572835141 2216 22-23 -0.7995258572840385 2216 24-25 -0.8137999185048486 2216 26-27 -0.8643926543209872 2216 28-29 -0.9082661574508464 2216 30-31 -0.9651450645595716 2216 32-33 -0.9860556025154494 2216 34-35 -0.9266893306900172 2216 36-37 -1.0200649476306154 2216 38-39 -0.9084851085981853 2216 40-41 -0.8649900449903143 2216 42-43 -0.9321400724873854 2216 44-45 -0.9872503273971063 2216 46-47 -0.9671777886034008 2216 48-49 -0.9076059128167273 2216 50-51 -0.9025229855860886 2216 52-53 -0.9025056420429962 2216 54-55 -0.8980324225938006 2216 56-57 -0.8989722782073031 2216 58-59 -0.9424809372064686 2216 60-61 -0.9733424283076388 2216 62-63 -0.9654866741827206 2216 64-65 -0.9854381310755826 2216 66-67 -0.9475577974034124 2216 68-69 -0.9751034115884991 2216 70-71 -0.9739317023962215 2216 72-73 -0.9692723663991387 2216 74-75 -0.900740109375981 2216 76-77 -0.8304163184989903 2216 78-79 -0.921104392834728 2216 80-81 -0.9823508180062106 2216 82-83 -0.9251814088893298 2216 84-85 -1.069119068636244 2216 86-87 -1.0189787233455831 2216 88-89 -1.1374907032440191 2216 90-91 -0.9992456889854253 2216 92-93 -1.0056931828025135 2216 94-95 -1.0432104057582983 2216 96-97 -1.0677837327353608 2216 98-99 -1.2158772355435943 2216 100 -1.3042494444214903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 374.0 3 59.0 4 87.0 5 208.0 6 507.0 7 1363.0 8 3114.0 9 4849.0 10 6125.0 11 8004.0 12 11668.0 13 15648.0 14 15658.0 15 19212.0 16 25147.0 17 35106.0 18 34942.0 19 21499.0 20 17911.0 21 18521.0 22 19718.0 23 22130.0 24 26171.0 25 32325.0 26 41922.0 27 54914.0 28 71987.0 29 94244.0 30 121806.0 31 157110.0 32 203490.0 33 266261.0 34 365875.0 35 540936.0 36 896407.0 37 1622166.0 38 1903644.0 39 573238.0 40 1336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7014331769208 19.102717059118806 13.637258378912847 23.55859138504755 2 16.58833725072295 20.367899254680676 37.90467939471437 25.139084099881998 3 18.310656393155046 25.35448769667689 30.08410511927066 26.2507507908974 4 13.312297865314385 17.288409277032812 36.72222680100919 32.6770660566436 5 13.500650644228068 36.34880043974481 34.35740595224033 15.7931429637868 6 30.941647485134744 36.61692768297156 17.9407793373382 14.500645494555506 7 27.49880714176834 31.667457311387132 22.127030925556 18.70670462128853 8 26.155419245451633 34.25480017167244 20.37484298503875 19.21493759783717 9 25.45469881397779 16.3900926198254 21.060253191912214 37.094955374284595 10-11 24.64732467602632 26.044305690354125 28.069346203430634 21.23902343018892 12-13 25.317181373353097 23.584865608168627 28.3897281782992 22.708224840179074 14-15 22.632017587322263 25.307114521710645 26.087847577642954 25.97302031332414 16-17 22.13768464494447 28.38782074517599 26.48540826348233 22.98908634639721 18-19 22.058213561085 27.054312824276096 28.945437991653574 21.94203562298533 20-21 22.995295273414683 26.30408829934939 28.812922065768593 21.88769436146733 22-23 22.694958241003395 26.21300106592323 28.81178502585973 22.28025566721364 24-25 22.44456376860112 26.315759007530517 28.847575809238528 22.392101414629835 26-27 22.38722247773089 26.284776379991452 28.855459289199537 22.47254185307812 28-29 22.418174693973857 26.26522068048565 28.959623444888834 22.35698118065166 30-31 22.58336449856571 26.53480586213714 28.65467564969603 22.227153989601113 32-33 22.325182221120247 26.34901101227432 28.94245629611519 22.383350470490242 34-35 22.450332367147894 26.081611085162095 28.929152205010116 22.538904342679896 36-37 22.78832897651619 26.31070779178627 28.60886624118845 22.292096990509087 38-39 22.63169954266817 26.42983817144448 28.59294572625763 22.345516559629726 40-41 22.537281136985094 26.243556772073738 28.723455279174388 22.49570681176678 42-43 22.429774901497698 26.395564130001535 28.62460768601871 22.550053282482054 44-45 22.566676782887434 26.520782218164708 28.399305150699988 22.513235848247866 46-47 22.717114227550983 26.567752889305545 28.075378742955774 22.639754140187698 48-49 22.713171205752417 26.499447431727308 28.04000929205037 22.747372070469908 50-51 22.72399472550251 26.535194728810275 27.93534847069549 22.805462074991723 52-53 22.700624587735984 26.642585256818602 27.860591984250654 22.79619817119476 54-55 22.73732661922837 26.94642073396249 27.762361865798617 22.553890781010523 56-57 22.501251607794874 26.7449273274995 27.778181676599704 22.975639388105918 58-59 22.529526356494433 26.37827048152699 28.002150315783698 23.09005284619488 60-61 22.709491349220347 26.669226042427063 27.859632090768116 22.761650517584474 62-63 22.533295909668297 26.721330669021974 27.913448517457102 22.831924903852627 64-65 22.5249272276077 26.442656126680504 28.02992500830902 23.002491637402773 66-67 22.404783474168465 26.52396126768571 27.956776287490282 23.114478970655547 68-69 22.71531934262242 26.64600919490645 27.77420623038812 22.86446523208301 70-71 22.704655620731398 26.404361940085348 27.84139660145813 23.04958583772513 72-73 22.488792020405047 26.63107053097125 27.89123217175455 22.988905276869158 74-75 22.47815429007266 26.573186057005444 27.789581592737694 23.159078060184203 76-77 22.56649581392522 26.603163170397853 27.602155199176476 23.22818581650045 78-79 22.57625211526211 26.686303906967336 27.735858444275195 23.001585533495362 80-81 22.435897656770017 26.56242495025742 27.785851613053055 23.21582577991951 82-83 22.384308725988458 26.572576449306574 27.800561633215608 23.242553191489364 84-85 22.44016611748076 26.5965143004261 27.605157569586364 23.358162012506774 86-87 22.515142042878175 26.646687687595804 27.589438871297943 23.248731398228077 88-89 22.30788160417376 26.551880093342938 27.720885789004246 23.41935251347905 90-91 22.175724634310438 26.51508526514134 27.71430128851978 23.594888812028437 92-93 22.317143461207788 26.69280829196073 27.55360475747994 23.436443489351543 94-95 22.308931037020642 26.42879805236636 27.674440368191405 23.587830542421592 96-97 22.159684027407373 26.51204606229204 27.719698228918322 23.608571681382266 98-99 22.074112542728518 26.54250096805251 27.713136426043167 23.670250063175807 100 22.231251222326158 26.371730764221397 27.645306756832934 23.75171125661951 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1991.0 1 1567.5 2 6845.0 3 10015.0 4 4673.5 5 1763.5 6 2605.0 7 5904.0 8 10075.0 9 11468.0 10 8200.0 11 4561.0 12 3800.5 13 4086.5 14 4640.5 15 5207.5 16 5860.0 17 6520.5 18 7095.5 19 8019.0 20 9277.5 21 10724.0 22 13027.5 23 16805.5 24 21927.5 25 28368.0 26 37366.0 27 48535.5 28 61590.0 29 76229.5 30 89271.0 31 102138.5 32 115638.0 33 127856.5 34 140201.0 35 153316.0 36 167374.5 37 182696.0 38 197047.0 39 208234.0 40 218790.0 41 228615.0 42 235240.5 43 240495.0 44 244130.5 45 247099.0 46 249520.5 47 248215.0 48 244900.0 49 245118.0 50 247728.5 51 246759.5 52 239543.5 53 234316.5 54 231557.0 55 221940.5 56 208409.5 57 190590.5 58 173589.5 59 161986.0 60 146078.5 61 125505.5 62 106951.5 63 89481.5 64 72379.0 65 57769.0 66 44828.5 67 33946.0 68 25845.0 69 19749.0 70 14695.0 71 10857.5 72 8282.0 73 6141.5 74 4590.5 75 3224.0 76 2027.5 77 1298.5 78 859.0 79 527.5 80 283.0 81 133.0 82 68.0 83 34.0 84 17.5 85 12.0 86 8.5 87 4.0 88 2.5 89 3.5 90 2.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08631855695991086 2 0.0 3 0.0 4 0.0 5 0.007787000587953 6 6.891150962790265E-5 7 0.0 8 5.5129207702322125E-5 9 0.0 10-11 0.0 12-13 0.013782301925580531 14-15 1.3782301925580531E-5 16-17 0.0 18-19 2.0673452888370798E-5 20-21 0.0 22-23 0.0 24-25 4.1346905776741595E-5 26-27 4.1346905776741595E-5 28-29 1.5160532118138585E-4 30-31 6.891150962790266E-6 32-33 7.580266059069292E-5 34-35 1.3782301925580531E-5 36-37 4.1346905776741595E-5 38-39 1.3093186829301505E-4 40-41 2.7564603851161062E-5 42-43 1.9295222695812743E-4 44-45 1.1025841540464425E-4 46-47 3.445575481395133E-5 48-49 2.411902836976593E-4 50-51 1.9984337792091768E-4 52-53 2.205168308092885E-4 54-55 1.584964721441761E-4 56-57 1.9984337792091768E-4 58-59 4.823805673953186E-5 60-61 8.958496251627345E-5 62-63 3.2388409525114245E-4 64-65 1.584964721441761E-4 66-67 4.823805673953186E-4 68-69 4.754894164325283E-4 70-71 1.584964721441761E-4 72-73 3.514486991023035E-4 74-75 3.3077524621393276E-4 76-77 2.5497258562323985E-4 78-79 0.0011025841540464425 80-81 0.001068128399232491 82-83 7.855912097580902E-4 84-85 0.0012955363810045698 86-87 7.718089078325097E-4 88-89 9.85434587679008E-4 90-91 0.0014953797589254876 92-93 0.0010474549463441204 94-95 0.0014884886079626974 96-97 0.0015987470233673415 98-99 0.001867501910916162 100 6.75332794353446E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7255682.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.162019569612184 #Duplication Level Percentage of deduplicated Percentage of total 1 73.92128883871833 40.037262926936165 2 14.712017545773303 15.93665164445303 3 4.718037188460132 7.666152675946073 4 2.125762884744116 4.6054244385546435 5 1.1670526954501876 3.1604965464870856 6 0.7434028114027025 2.4158518571578735 7 0.514774829371737 1.9516871067663057 8 0.33740569524418107 1.4619659094971154 9 0.26653001306123747 1.2992223404980563 >10 1.3705308302976422 13.298697936218456 >50 0.08595602096866026 3.191145819068021 >100 0.035914719200078264 3.3609339486781367 >500 9.179875454278733E-4 0.33347558152170464 >1k 2.8045858860493967E-4 0.2886138647337521 >5k 2.5496234731373364E-5 0.09583637297436166 >10k+ 1.0198493892549346E-4 0.8965810305091362 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20324 0.2801115043354987 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18535 0.2554549661906351 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15112 0.20827814669937297 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10955 0.1509851175947347 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 1.3782301925580531E-5 0.0 0.0 0.0 8 0.0 1.3782301925580531E-5 0.0 0.0 0.0 9 0.0 2.7564603851161062E-5 0.0 0.0 0.0 10-11 0.0 2.7564603851161062E-5 6.891150962790266E-6 0.0 0.0 12-13 0.0 4.1346905776741595E-5 1.3782301925580531E-5 1.3782301925580531E-5 0.0 14-15 0.0 6.891150962790265E-5 1.3782301925580531E-5 2.0673452888370798E-5 0.0 16-17 0.0 8.958496251627345E-5 1.3782301925580531E-5 2.7564603851161062E-5 0.0 18-19 0.0 1.0336726444185398E-4 1.3782301925580531E-5 4.8238056739531864E-5 0.0 20-21 0.0 1.1025841540464425E-4 1.3782301925580531E-5 1.0336726444185398E-4 0.0 22-23 0.0 1.1025841540464425E-4 1.3782301925580531E-5 2.6875488754882036E-4 0.0 24-25 0.0 1.3093186829301505E-4 1.3782301925580531E-5 6.408770395394947E-4 0.0 26-27 6.891150962790266E-6 1.378230192558053E-4 1.3782301925580531E-5 0.0012404071733022478 6.891150962790266E-6 28-29 1.3782301925580531E-5 1.4471417021859556E-4 1.3782301925580531E-5 0.002680657724525413 1.3782301925580531E-5 30-31 1.3782301925580531E-5 1.5160532118138585E-4 1.3782301925580531E-5 0.00651902881079959 1.3782301925580531E-5 32-33 2.7564603851161062E-5 1.5160532118138585E-4 1.3782301925580531E-5 0.015263899382580437 1.3782301925580531E-5 34-35 2.7564603851161062E-5 1.6538762310696638E-4 1.3782301925580531E-5 0.029308065044747 1.3782301925580531E-5 36-37 2.7564603851161062E-5 1.8606107599533717E-4 1.3782301925580531E-5 0.051035864030424705 1.3782301925580531E-5 38-39 2.7564603851161062E-5 1.998433779209177E-4 1.3782301925580531E-5 0.08350696736709244 1.3782301925580531E-5 40-41 2.7564603851161062E-5 2.0673452888370796E-4 1.3782301925580531E-5 0.1238133093484527 1.3782301925580531E-5 42-43 2.7564603851161062E-5 2.3429913273486904E-4 2.0673452888370798E-5 0.16570461605125472 1.3782301925580531E-5 44-45 2.7564603851161062E-5 2.3429913273486904E-4 2.7564603851161062E-5 0.21589286851325623 1.3782301925580531E-5 46-47 2.7564603851161062E-5 2.411902836976593E-4 2.7564603851161062E-5 0.2749638145662944 1.3782301925580531E-5 48-49 2.7564603851161062E-5 2.756460385116106E-4 2.7564603851161062E-5 0.3424902028506762 1.3782301925580531E-5 50-51 2.7564603851161062E-5 2.756460385116106E-4 2.7564603851161062E-5 0.41333812589912294 1.3782301925580531E-5 52-53 2.7564603851161062E-5 2.756460385116106E-4 3.445575481395133E-5 0.48484070828903475 1.3782301925580531E-5 54-55 2.7564603851161062E-5 2.825371894744009E-4 4.1346905776741595E-5 0.5660046843287785 1.3782301925580531E-5 56-57 2.7564603851161062E-5 2.894283404371912E-4 4.1346905776741595E-5 0.6618123561644516 1.3782301925580531E-5 58-59 2.7564603851161062E-5 2.894283404371912E-4 4.1346905776741595E-5 0.7679085163875705 1.3782301925580531E-5 60-61 2.7564603851161062E-5 2.894283404371912E-4 4.1346905776741595E-5 0.8796209646453634 1.3782301925580531E-5 62-63 2.7564603851161062E-5 2.894283404371912E-4 4.1346905776741595E-5 0.9941450024959749 1.3782301925580531E-5 64-65 2.7564603851161062E-5 2.894283404371912E-4 4.1346905776741595E-5 1.1180823525617578 1.3782301925580531E-5 66-67 2.7564603851161062E-5 3.1010179332556195E-4 4.1346905776741595E-5 1.2592475800345164 1.3782301925580531E-5 68-69 2.7564603851161062E-5 3.169929442883522E-4 4.1346905776741595E-5 1.4161866520611017 1.3782301925580531E-5 70-71 2.7564603851161062E-5 3.2388409525114245E-4 4.1346905776741595E-5 1.5833177363616542 2.0673452888370798E-5 72-73 2.7564603851161062E-5 3.5833985006509384E-4 4.1346905776741595E-5 1.763314599509736 2.7564603851161062E-5 74-75 4.1346905776741595E-5 3.5833985006509384E-4 4.1346905776741595E-5 1.9471360514421663 2.7564603851161062E-5 76-77 4.1346905776741595E-5 3.7212215199067435E-4 4.8238056739531864E-5 2.1419282157073587 2.7564603851161062E-5 78-79 4.1346905776741595E-5 3.7212215199067435E-4 5.5129207702322125E-5 2.3556917185731128 2.7564603851161062E-5 80-81 4.1346905776741595E-5 3.7212215199067435E-4 5.5129207702322125E-5 2.5858285961264564 2.7564603851161062E-5 82-83 4.1346905776741595E-5 3.7212215199067435E-4 6.891150962790265E-5 2.8304437818526225 2.7564603851161062E-5 84-85 4.8238056739531864E-5 3.790133029534646E-4 6.891150962790265E-5 3.0752312463528586 2.7564603851161062E-5 86-87 5.5129207702322125E-5 3.8590445391625485E-4 6.891150962790265E-5 3.3358476846146234 2.7564603851161062E-5 88 5.5129207702322125E-5 3.8590445391625485E-4 6.891150962790265E-5 3.543677906501415 2.7564603851161062E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11360 0.0 51.75691 1 TATAACG 855 0.0 38.480106 2 GTATCAA 19515 0.0 38.01017 1 TATCACG 1250 0.0 33.464497 2 GTACCTG 7100 0.0 32.660683 1 TACCTGG 6895 0.0 32.51535 2 ATAACGC 1065 0.0 32.216446 3 ATCAACG 23335 0.0 31.441223 3 TAACGCA 1085 0.0 31.189405 4 TCAACGC 23500 0.0 31.180464 4 CAACGCA 24475 0.0 30.015154 5 TATCAAC 25070 0.0 29.37778 2 AACGCAG 25810 0.0 28.70591 6 ACCTGGG 8685 0.0 25.597387 3 ACGCAGA 29445 0.0 25.162151 7 CGCAGAG 30145 0.0 24.577858 8 GCAGAGT 33445 0.0 21.984097 9 GTACATG 21875 0.0 21.717506 1 TCACGCA 1975 0.0 20.942085 4 AGAGTAC 31290 0.0 20.718668 10-11 >>END_MODULE