FastQCFastQC Report
Wed 25 May 2016
SRR2049482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9647871
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG11450.040.2290762
GTATCAA133400.032.7076231
GGTATCA85150.032.5227661
TAACGCA15350.032.4573024
TACCTGG87850.032.315782
ATAACGC15500.031.5372143
TCAACGC136000.030.240164
GTACCTG96250.030.1887131
ATCAACG137450.030.0925983
CAACGCA147100.027.9267565
AACGCAG160200.026.1996146
TATCAAC165450.025.2270012
TATCACG15550.025.0880642
GTACATG296850.023.441291
ACCTGGG120250.023.2569943
GTATAAC30700.022.0536751
ACGCAGA190850.022.0169987
TACATGG308100.021.6171072
ACATGGG303700.020.9863363
CGCAGAG198900.020.9122588
Produced by FastQC (version 0.11.5)