FastQCFastQC Report
Wed 25 May 2016
SRR2049482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9647871
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238330.24702859314764883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204480.2119431323242195No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146380.15172259247661996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121160.12558211029148295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136500.046.569261
TATAACG14050.039.475682
GTATCAA237550.039.039861
ATAACGC16800.034.6925623
TAACGCA17050.034.459554
TACCTGG91650.032.1557772
ATCAACG283500.031.9818153
TCAACGC284150.031.8259494
CAACGCA296300.030.600225
GTACCTG100600.030.4480631
TATCACG16700.029.834132
TATCAAC307450.029.5363332
AACGCAG313650.029.3625766
ACGCAGA362750.025.3882067
CGCAGAG372200.024.7436088
ACCTGGG118900.024.6280573
GTATAAC34050.024.5968511
GTACATG302300.022.3663561
GCAGAGT421350.021.801519
GTGGTAT71950.021.3842281
Produced by FastQC (version 0.11.5)