##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049482_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9647871 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82666041036411 31.0 31.0 34.0 30.0 34.0 2 32.00874099581141 33.0 31.0 34.0 30.0 34.0 3 32.03744795095208 34.0 31.0 34.0 30.0 34.0 4 35.71920758476145 37.0 35.0 37.0 33.0 37.0 5 35.77758098133775 37.0 35.0 37.0 35.0 37.0 6 35.83016004256276 37.0 35.0 37.0 35.0 37.0 7 35.79104011651897 37.0 35.0 37.0 35.0 37.0 8 35.80570076030246 37.0 35.0 37.0 35.0 37.0 9 37.533499566899266 39.0 37.0 39.0 35.0 39.0 10-11 37.49275860964559 39.0 37.0 39.0 35.0 39.0 12-13 37.459571702399415 39.0 37.0 39.0 34.5 39.0 14-15 38.74836272168233 40.0 38.0 41.0 35.0 41.0 16-17 38.75426257254061 40.0 38.0 41.0 35.0 41.0 18-19 38.80523200403488 40.0 38.0 41.0 35.0 41.0 20-21 38.81341857701041 40.0 38.0 41.0 35.0 41.0 22-23 38.7751189355662 40.0 38.0 41.0 35.0 41.0 24-25 38.747522847268584 40.0 38.0 41.0 34.0 41.0 26-27 38.64206875278494 40.0 38.0 41.0 34.0 41.0 28-29 38.56442955134868 40.0 38.0 41.0 34.0 41.0 30-31 38.47121131698382 40.0 38.0 41.0 34.0 41.0 32-33 38.359479049833894 40.0 38.0 41.0 34.0 41.0 34-35 38.25429444485732 40.0 38.0 41.0 34.0 41.0 36-37 38.145352275128886 40.0 38.0 41.0 33.5 41.0 38-39 38.06228208275173 40.0 38.0 41.0 33.0 41.0 40-41 37.98574799559405 40.0 37.5 41.0 33.0 41.0 42-43 37.86386416236287 40.0 37.0 41.0 33.0 41.0 44-45 37.70810295867347 40.0 37.0 41.0 33.0 41.0 46-47 37.50372056176953 40.0 37.0 41.0 33.0 41.0 48-49 37.30238075322524 40.0 37.0 41.0 32.0 41.0 50-51 37.145941213351634 40.0 36.0 41.0 32.0 41.0 52-53 37.00662628055454 40.0 36.0 41.0 31.5 41.0 54-55 36.84342695917058 39.0 35.5 41.0 31.0 41.0 56-57 36.66710790390958 39.0 35.0 41.0 31.0 41.0 58-59 36.45842518002158 39.0 35.0 41.0 31.0 41.0 60-61 36.23699181923141 39.0 35.0 40.5 31.0 41.0 62-63 35.986663275244865 38.0 35.0 40.0 30.5 41.0 64-65 35.735629031524155 37.5 35.0 40.0 30.0 41.0 66-67 35.44271150598925 37.0 35.0 40.0 30.0 41.0 68-69 35.12521265054228 36.5 34.0 39.5 29.5 41.0 70-71 34.81079675505612 36.0 34.0 39.0 29.0 41.0 72-73 34.478657571188506 35.5 34.0 39.0 29.0 40.5 74-75 34.160815375744555 35.0 34.0 37.5 29.0 40.0 76-77 33.85235592391316 35.0 34.0 37.0 29.0 39.0 78-79 33.55667929225007 35.0 34.0 37.0 29.0 39.0 80-81 33.27386933345191 35.0 34.0 36.0 29.0 39.0 82-83 33.02634705625728 35.0 33.5 36.0 29.0 37.5 84-85 32.78138358193222 35.0 33.0 35.5 28.0 37.0 86-87 32.57209652782464 35.0 33.0 35.0 27.5 37.0 88-89 32.37963976715692 35.0 33.0 35.0 27.0 36.0 90-91 32.21066580388565 35.0 33.0 35.0 27.0 36.0 92-93 32.057745952449 35.0 33.0 35.0 27.0 36.0 94-95 31.906438062863817 35.0 33.0 35.0 27.0 35.5 96-97 31.748435794798667 35.0 33.0 35.0 26.0 35.0 98-99 31.605943736188017 35.0 33.0 35.0 26.0 35.0 100 31.490319885081384 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2572510765201699 1101 2 0.30648292135305155 1101 3 0.4280035071041297 1101 4 0.2636706151820718 1101 5 0.20771380925408067 1101 6 0.22734747017872792 1101 7 0.19119321401630174 1101 8 0.21689312016281548 1101 9 0.3213935516953157 1101 10-11 0.3071036482965894 1101 12-13 0.27691697445605 1101 14-15 0.35766895875197946 1101 16-17 0.3410411293963378 1101 18-19 0.35307857963191935 1101 20-21 0.37508769895681837 1101 22-23 0.3668182680496983 1101 24-25 0.3359158205678838 1101 26-27 0.38336004876683205 1101 28-29 0.40202465498305884 1101 30-31 0.40962944719161953 1101 32-33 0.38281113293781743 1101 34-35 0.3707911820155303 1101 36-37 0.41727033602742836 1101 38-39 0.39220475521785403 1101 40-41 0.4278931597568061 1101 42-43 0.46233674979743 1101 44-45 0.4927619334297262 1101 46-47 0.46670002575283576 1101 48-49 0.4841425979898588 1101 50-51 0.46529099060147416 1101 52-53 0.4698325349741026 1101 54-55 0.44996252415513993 1101 56-57 0.47212370851531915 1101 58-59 0.47026511391410963 1101 60-61 0.4533971860527757 1101 62-63 0.4488169542089153 1101 64-65 0.44126537242474484 1101 66-67 0.47049074846717787 1101 68-69 0.44903391624126243 1101 70-71 0.46468377390913673 1101 72-73 0.4470182443810913 1101 74-75 0.45299272314885286 1101 76-77 0.44076676184042896 1101 78-79 0.4139462502171867 1101 80-81 0.4020783576432194 1101 82-83 0.4095756020921044 1101 84-85 0.42690396289980015 1101 86-87 0.39646431677480365 1101 88-89 0.42759932298071845 1101 90-91 0.4549246553890711 1101 92-93 0.45449668151557887 1101 94-95 0.4420489149317888 1101 96-97 0.40542207094858185 1101 98-99 0.48324074397181604 1101 100 0.4760742460314269 1102 1 0.25140975696500334 1102 2 0.2320525283436794 1102 3 0.35778779283450746 1102 4 0.24109806551842183 1102 5 0.21356202073278752 1102 6 0.17222906514900416 1102 7 0.20924605700609078 1102 8 0.17916580758311085 1102 9 0.22161687729199997 1102 10-11 0.262414040077374 1102 12-13 0.21719174862911927 1102 14-15 0.3099817519222938 1102 16-17 0.27074077139604924 1102 18-19 0.31429388653325674 1102 20-21 0.29537860652496306 1102 22-23 0.29256255435660705 1102 24-25 0.28747263161674397 1102 26-27 0.30899599970027936 1102 28-29 0.29597895265545304 1102 30-31 0.29925555394065384 1102 32-33 0.3033779167573982 1102 34-35 0.28897146684687414 1102 36-37 0.31444128952998085 1102 38-39 0.3202255844360522 1102 40-41 0.34898286958626556 1102 42-43 0.29180058101415796 1102 44-45 0.31184214297860535 1102 46-47 0.29263541765163126 1102 48-49 0.30343885413125093 1102 50-51 0.2924344045230356 1102 52-53 0.29418718500946284 1102 54-55 0.3191568679440522 1102 56-57 0.32091746559488143 1102 58-59 0.30543961710344547 1102 60-61 0.3061061746127507 1102 62-63 0.299728399033647 1102 64-65 0.2539765844430377 1102 66-67 0.2759632378700374 1102 68-69 0.26232061961017195 1102 70-71 0.2618748723888089 1102 72-73 0.25117977348765663 1102 74-75 0.2723001039248203 1102 76-77 0.2198426071338062 1102 78-79 0.2828139881622107 1102 80-81 0.29906445653902836 1102 82-83 0.27682081654285184 1102 84-85 0.19879275402431063 1102 86-87 0.20255062270734925 1102 88-89 0.19759011822414152 1102 90-91 0.23027250503654528 1102 92-93 0.25092783265752416 1102 94-95 0.202233861430841 1102 96-97 0.24573262018412123 1102 98-99 0.30209684537074466 1102 100 0.2885260196128776 1103 1 0.24586533488007234 1103 2 0.2315721247470819 1103 3 0.336179574948801 1103 4 0.18879427835562268 1103 5 0.18314104363791017 1103 6 0.161349758826681 1103 7 0.16121654994439183 1103 8 0.1764589265292713 1103 9 0.22382315033487998 1103 10-11 0.20340596063937255 1103 12-13 0.19745626249416404 1103 14-15 0.2607336195391312 1103 16-17 0.2531722878240288 1103 18-19 0.2600473770372602 1103 20-21 0.30278752647476637 1103 22-23 0.2844757502710564 1103 24-25 0.2810709155054525 1103 26-27 0.24631854235854433 1103 28-29 0.30688459414039926 1103 30-31 0.294535321883302 1103 32-33 0.30144534257370736 1103 34-35 0.2526976976652904 1103 36-37 0.23638957966480234 1103 38-39 0.24686344267747273 1103 40-41 0.2734217085677173 1103 42-43 0.32743699740159826 1103 44-45 0.31014903551599105 1103 46-47 0.29316884890268824 1103 48-49 0.272003230540939 1103 50-51 0.2605441050391022 1103 52-53 0.2805609920761185 1103 54-55 0.27073820895605394 1103 56-57 0.2847423550076158 1103 58-59 0.2659353213325488 1103 60-61 0.24060932856369277 1103 62-63 0.24082591037660706 1103 64-65 0.24184631450593486 1103 66-67 0.27739060470538135 1103 68-69 0.2524397319325402 1103 70-71 0.23563413655690368 1103 72-73 0.3025061376490825 1103 74-75 0.3111045398136909 1103 76-77 0.27974374925187817 1103 78-79 0.25214013939626767 1103 80-81 0.2120716041849704 1103 82-83 0.22870142076654076 1103 84-85 0.2626652478550753 1103 86-87 0.23450134656036425 1103 88-89 0.23929571505723857 1103 90-91 0.23652326997628847 1103 92-93 0.2544683882770755 1103 94-95 0.20668895521587416 1103 96-97 0.2602265513661024 1103 98-99 0.31079108869102257 1103 100 0.27325247266964325 1104 1 0.2038835035236346 1104 2 0.2226212360494202 1104 3 0.31643613829405126 1104 4 0.2010790273783698 1104 5 0.17444240331814598 1104 6 0.17176475455292461 1104 7 0.15414674111015358 1104 8 0.14631773602959441 1104 9 0.18911663186398897 1104 10-11 0.2012601741627975 1104 12-13 0.1617434932013353 1104 14-15 0.2443507733143946 1104 16-17 0.23536470822776323 1104 18-19 0.2519904890287208 1104 20-21 0.23710927919398728 1104 22-23 0.22222407610860273 1104 24-25 0.2476822322243919 1104 26-27 0.2563836128925132 1104 28-29 0.268875276922266 1104 30-31 0.26740799889684297 1104 32-33 0.2769363097156514 1104 34-35 0.2695232708276265 1104 36-37 0.2868041611707852 1104 38-39 0.24902118085653768 1104 40-41 0.2244489145268318 1104 42-43 0.29805011820742067 1104 44-45 0.28265051296089183 1104 46-47 0.27351088532889634 1104 48-49 0.26203829535845813 1104 50-51 0.2826341943582662 1104 52-53 0.31270340954147 1104 54-55 0.2752252393154251 1104 56-57 0.2656654215848846 1104 58-59 0.3382210958741325 1104 60-61 0.32999431268287793 1104 62-63 0.3262647917174135 1104 64-65 0.25306140221655227 1104 66-67 0.2934200739023254 1104 68-69 0.2731861430070168 1104 70-71 0.2672150513272129 1104 72-73 0.2971666620548987 1104 74-75 0.2930659911576754 1104 76-77 0.2635898385431332 1104 78-79 0.24001727577606147 1104 80-81 0.2173843200235268 1104 82-83 0.23728576505526888 1104 84-85 0.20778411226810078 1104 86-87 0.18382249578903043 1104 88-89 0.20848389493664143 1104 90-91 0.185382903607902 1104 92-93 0.23654110880993073 1104 94-95 0.2691539958065903 1104 96-97 0.28765762105241777 1104 98-99 0.29812769283246965 1104 100 0.2677875770906084 1105 1 0.20087909340175258 1105 2 0.1898843607202778 1105 3 0.2985494721823514 1105 4 0.1795708310645523 1105 5 0.14952317314261165 1105 6 0.1596505511323869 1105 7 0.13900709762374674 1105 8 0.13377430372047883 1105 9 0.18026328958349325 1105 10-11 0.17076164077927558 1105 12-13 0.15766219050622965 1105 14-15 0.21277857220520247 1105 16-17 0.26261770750166136 1105 18-19 0.25216708725091763 1105 20-21 0.2643746543906147 1105 22-23 0.2303055296391605 1105 24-25 0.24840343347860738 1105 26-27 0.21827784425161667 1105 28-29 0.2813433371245466 1105 30-31 0.2467039926353607 1105 32-33 0.28275271005409763 1105 34-35 0.27077456690177826 1105 36-37 0.2806965808715276 1105 38-39 0.28155150746209046 1105 40-41 0.269911871478719 1105 42-43 0.23164211504925447 1105 44-45 0.24118337263015377 1105 46-47 0.25799403313047975 1105 48-49 0.3087672775094532 1105 50-51 0.27300966688728323 1105 52-53 0.287257208542016 1105 54-55 0.2952219330182757 1105 56-57 0.25970546390448845 1105 58-59 0.2684708876181361 1105 60-61 0.26119692801454164 1105 62-63 0.26061212805434053 1105 64-65 0.2397689552436404 1105 66-67 0.22959732272250477 1105 68-69 0.26948047862162383 1105 70-71 0.2935386677891003 1105 72-73 0.33817657999546924 1105 74-75 0.31048103472231503 1105 76-77 0.27015297237996094 1105 78-79 0.2527635277036069 1105 80-81 0.29518668924481517 1105 82-83 0.22557949644438224 1105 84-85 0.24461643111674647 1105 86-87 0.21964638824311322 1105 88-89 0.2487346386229774 1105 90-91 0.23039815663251773 1105 92-93 0.20925515269873074 1105 94-95 0.24985642647911277 1105 96-97 0.286100545558746 1105 98-99 0.22327779039575546 1105 100 0.24351333083064475 1106 1 0.1891258989153144 1106 2 0.15945673487124168 1106 3 0.2661179595082004 1106 4 0.15238456888476293 1106 5 0.15954826434252567 1106 6 0.13567554840528828 1106 7 0.14130892991421007 1106 8 0.11297263053232598 1106 9 0.17040556943294405 1106 10-11 0.14432375869648695 1106 12-13 0.13660865858673077 1106 14-15 0.19306626824579354 1106 16-17 0.20963686772786616 1106 18-19 0.17715597247569548 1106 20-21 0.1804654840586295 1106 22-23 0.18401951085347434 1106 24-25 0.16565891623528728 1106 26-27 0.15790921287324977 1106 28-29 0.19695860878140792 1106 30-31 0.21955881303397717 1106 32-33 0.1537590289548021 1106 34-35 0.1475003441653655 1106 36-37 0.13542464045912794 1106 38-39 0.15697260622262377 1106 40-41 0.1763548150806713 1106 42-43 0.21172598385754782 1106 44-45 0.16855727898547457 1106 46-47 0.14846713756636376 1106 48-49 0.11258503315826118 1106 50-51 0.09989735166969638 1106 52-53 0.09406945229765284 1106 54-55 0.10109350745107548 1106 56-57 0.10821331145113788 1106 58-59 0.09832218444762475 1106 60-61 0.09180112173944366 1106 62-63 0.05710943133680502 1106 64-65 0.05950859084691018 1106 66-67 0.03959547402979524 1106 68-69 0.09038519637442022 1106 70-71 0.1353088069153543 1106 72-73 0.1114537921668628 1106 74-75 0.10492417643845897 1106 76-77 0.09000782481822966 1106 78-79 0.05141116747939378 1106 80-81 0.06688602073532479 1106 82-83 0.12044048048895917 1106 84-85 0.13897435761293053 1106 86-87 0.11090810656008188 1106 88-89 0.09795361259013191 1106 90-91 0.1169444516709035 1106 92-93 0.15152892067725077 1106 94-95 0.11941322851868819 1106 96-97 0.1319922014394308 1106 98-99 0.14328898888448904 1106 100 0.130482285804856 1107 1 0.0926665953417185 1107 2 0.09767807070507928 1107 3 0.19941813791437113 1107 4 0.12449707073076866 1107 5 0.1488619605528143 1107 6 0.0854923430709249 1107 7 0.06399201950141276 1107 8 0.1181691243724714 1107 9 0.12668079933973786 1107 10-11 0.10393182509308474 1107 12-13 0.08040015485803309 1107 14-15 0.14273105441001377 1107 16-17 0.12467102137458141 1107 18-19 0.13787965619260234 1107 20-21 0.12490258869972592 1107 22-23 0.13463972347322795 1107 24-25 0.11075613881587998 1107 26-27 0.16294429432722524 1107 28-29 0.1822634616342853 1107 30-31 0.19328788407199227 1107 32-33 0.15595399081496453 1107 34-35 0.11101672087853842 1107 36-37 0.13935365716459813 1107 38-39 0.11259028246985991 1107 40-41 0.16695103555622381 1107 42-43 0.18405151308741097 1107 44-45 0.15035182475977393 1107 46-47 0.1661810666800605 1107 48-49 0.1844894028559665 1107 50-51 0.16897617081809813 1107 52-53 0.1102819875044645 1107 54-55 0.13850065123759947 1107 56-57 0.12022538227851243 1107 58-59 0.1335383786371409 1107 60-61 0.141141808997574 1107 62-63 0.17978991318170046 1107 64-65 0.12191775448996367 1107 66-67 0.1495858491731994 1107 68-69 0.15213355811750517 1107 70-71 0.16345454704477191 1107 72-73 0.16115311490415962 1107 74-75 0.16610411669645941 1107 76-77 0.1287788338424818 1107 78-79 0.16973622557714663 1107 80-81 0.14915129373301284 1107 82-83 0.16679667759181882 1107 84-85 0.1638845300405123 1107 86-87 0.1266011063000434 1107 88-89 0.15900477155316395 1107 90-91 0.15878259111906345 1107 92-93 0.1726905390293183 1107 94-95 0.182569535494423 1107 96-97 0.17808974939306665 1107 98-99 0.23083605177060917 1107 100 0.18343127177740115 1108 1 0.039287169514089726 1108 2 0.09392370214627732 1108 3 0.17541406950360994 1108 4 0.09878759519320113 1108 5 0.08080339376969903 1108 6 0.08205202122399413 1108 7 0.05016803017009863 1108 8 0.0585595215194985 1108 9 0.087385831996734 1108 10-11 0.06514773757292147 1108 12-13 0.035814980086620096 1108 14-15 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-0.3361264720822277 2215 2 -0.3648307202507972 2215 3 -0.2712264543204057 2215 4 -0.2470272266684148 2215 5 -0.2274208103102282 2215 6 -0.21409053957759028 2215 7 -0.22469845334579475 2215 8 -0.2307065798538801 2215 9 -0.35591496649053767 2215 10-11 -0.3688808697773709 2215 12-13 -0.37340942234944663 2215 14-15 -0.4874741859545324 2215 16-17 -0.5142488993031264 2215 18-19 -0.4635390032063711 2215 20-21 -0.4671629537201696 2215 22-23 -0.46115835325097976 2215 24-25 -0.4518186210653923 2215 26-27 -0.43068091795912267 2215 28-29 -0.4173615470633294 2215 30-31 -0.46629143544343066 2215 32-33 -0.44245244255571947 2215 34-35 -0.4671967348626538 2215 36-37 -0.46913220637042485 2215 38-39 -0.4808152715574394 2215 40-41 -0.5345961611142513 2215 42-43 -0.48995472068570933 2215 44-45 -0.4662307544549762 2215 46-47 -0.4969815482119415 2215 48-49 -0.4538550955337257 2215 50-51 -0.4547372340094 2215 52-53 -0.48211572468953534 2215 54-55 -0.40733936403717763 2215 56-57 -0.3999223437755077 2215 58-59 -0.4023378608603849 2215 60-61 -0.4042570602028448 2215 62-63 -0.4322871751836672 2215 64-65 -0.44133393294735157 2215 66-67 -0.37662553017444367 2215 68-69 -0.39357277565262194 2215 70-71 -0.3619386324421825 2215 72-73 -0.3660558475459723 2215 74-75 -0.3616109926992763 2215 76-77 -0.30816788026125863 2215 78-79 -0.37503797199352107 2215 80-81 -0.4369208660473305 2215 82-83 -0.47076649289718375 2215 84-85 -0.4324465340043062 2215 86-87 -0.3943047663921888 2215 88-89 -0.3977298010833117 2215 90-91 -0.42201603587620085 2215 92-93 -0.3729583020711047 2215 94-95 -0.3903694788784371 2215 96-97 -0.37909189988838676 2215 98-99 -0.3611131961240055 2215 100 -0.37070483538018095 2216 1 -0.4201328945855778 2216 2 -0.4303296893508488 2216 3 -0.41028197500817853 2216 4 -0.30907232559525966 2216 5 -0.4028269885792781 2216 6 -0.3607906612538585 2216 7 -0.43392839046077825 2216 8 -0.35563968324225925 2216 9 -0.533244040352109 2216 10-11 -0.4675477470514622 2216 12-13 -0.421590183926817 2216 14-15 -0.6791465998290604 2216 16-17 -0.6455446024837954 2216 18-19 -0.618977958451886 2216 20-21 -0.5900870481186402 2216 22-23 -0.5702453094429174 2216 24-25 -0.5756044560318898 2216 26-27 -0.6072644475068287 2216 28-29 -0.6275482476139516 2216 30-31 -0.6772060029699034 2216 32-33 -0.6941822230852353 2216 34-35 -0.7220857300970707 2216 36-37 -0.7614930038779235 2216 38-39 -0.7216860554673588 2216 40-41 -0.7567100379363367 2216 42-43 -0.826083665641633 2216 44-45 -0.8683106480777383 2216 46-47 -0.8498404651667926 2216 48-49 -0.8398860462678073 2216 50-51 -0.807549244210982 2216 52-53 -0.8095485942617273 2216 54-55 -0.8283937291880932 2216 56-57 -0.8237297773184977 2216 58-59 -0.8303755475978889 2216 60-61 -0.8224634297713962 2216 62-63 -0.8361414713152726 2216 64-65 -0.8395519061058039 2216 66-67 -0.7621200010636286 2216 68-69 -0.7783992525069579 2216 70-71 -0.8085366534268772 2216 72-73 -0.8062986378040549 2216 74-75 -0.8034489474388522 2216 76-77 -0.7174641615471344 2216 78-79 -0.7691288167788457 2216 80-81 -0.7994476909963524 2216 82-83 -0.8068024433442744 2216 84-85 -0.9591334111101553 2216 86-87 -0.8523638429765725 2216 88-89 -0.9341287316897038 2216 90-91 -0.7689774805801655 2216 92-93 -0.8491696377354785 2216 94-95 -0.861916873367786 2216 96-97 -0.9282483154275738 2216 98-99 -1.0048070777037807 2216 100 -0.9873360538622116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 507.0 3 60.0 4 113.0 5 224.0 6 612.0 7 1495.0 8 3150.0 9 5170.0 10 6993.0 11 9556.0 12 13769.0 13 18713.0 14 19257.0 15 23128.0 16 30285.0 17 42030.0 18 41609.0 19 26292.0 20 22496.0 21 22579.0 22 24913.0 23 27704.0 24 33099.0 25 41606.0 26 54076.0 27 70525.0 28 92821.0 29 122827.0 30 158847.0 31 204668.0 32 264974.0 33 345704.0 34 477261.0 35 706749.0 36 1182386.0 37 2156401.0 38 2593614.0 39 799865.0 40 1793.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.491545607159125 19.0775390542123 13.469033274864278 23.961882063764296 2 16.45686390292739 20.25757807085107 37.91365991522896 25.371898110992568 3 18.10946684506872 25.501543293852087 29.853861022810108 26.535128838269085 4 13.307572201443884 17.362012000993378 36.72981429679594 32.6006015007668 5 13.708125130545156 36.31469532898416 34.247326512937384 15.729853027533302 6 30.986705860159663 36.55620002777818 18.01952971954402 14.437564392518135 7 27.702339718265307 31.518632452693453 22.14621236125566 18.632815467785587 8 26.247214149621957 34.09398890655774 20.361620014879982 19.297176928940317 9 25.55959755266214 16.52306503683559 20.9958964003561 36.921441010146175 10-11 24.62577495076375 26.029561340527874 28.07440626019979 21.270257448508588 12-13 25.37278218146629 23.532908277903527 28.31144389439372 22.782865646236466 14-15 22.71668089174308 25.24604787057369 25.97666002599434 26.060611211688883 16-17 22.274162869714985 28.309354467944274 26.358411094012347 23.05807156832839 18-19 22.23798102586374 27.084423815826707 28.68940501893164 21.988190139377913 20-21 23.134669590490375 26.358796246030924 28.4998961169506 22.006638046528103 22-23 22.815665756724982 26.271163866100615 28.508771520680572 22.40439885649383 24-25 22.617042981318356 26.37879195254163 28.484490767181054 22.51967429895896 26-27 22.559271897244873 26.340283020011984 28.5162402184358 22.584204864307342 28-29 22.58690645318788 26.32602081321769 28.626779857231544 22.460292876362885 30-31 22.724672765870977 26.60322814241754 28.32697122126587 22.34512787044561 32-33 22.481299623984263 26.400683881697173 28.567193734781288 22.550822759537276 34-35 22.59223254378765 26.164679474814594 28.584452172806245 22.658635808591516 36-37 22.93257713179978 26.369750667219883 28.258504546444403 22.43916765453594 38-39 22.73650558118646 26.493836611832737 28.29516890742288 22.474488899557922 40-41 22.676555689315656 26.33573661003603 28.403944518528156 22.583763182120155 42-43 22.567342184836356 26.45934800208917 28.293092774112537 22.680217038961935 44-45 22.682449786792098 26.564694139425736 28.125807173818856 22.62704889996331 46-47 22.82877137506145 26.61834475952042 27.822434741274936 22.730449124143192 48-49 22.826057157119394 26.5428511000566 27.789300501267405 22.841791241556596 50-51 22.80765990111997 26.57458089380093 27.70487382011314 22.91288538496596 52-53 22.81605109863591 26.654812903661806 27.61459145744433 22.914544540257957 54-55 22.8706152867299 26.907861299482068 27.555191951285735 22.6663314625023 56-57 22.627500155215913 26.74179980598788 27.592046145820454 23.038653892975752 58-59 22.653496638531255 26.37363537180139 27.801920134462893 23.170947855204457 60-61 22.8622067466025 26.646552368822384 27.60693598121491 22.88430490336021 62-63 22.63886410642879 26.728603152176905 27.69930742532487 22.933225316069436 64-65 22.650832062973567 26.465684220563997 27.794404094380543 23.089079622081897 66-67 22.527680995690268 26.53928693421348 27.7312764816962 23.201755588400054 68-69 22.826859771460253 26.665406278113547 27.55252910282257 22.955204847603632 70-71 22.850914677454973 26.427632174593846 27.610402913932834 23.11105023401835 72-73 22.62766841616116 26.624700081660812 27.651681835960183 23.095949666217845 74-75 22.612203129053693 26.59236987523732 27.59360465978022 23.201822335928767 76-77 22.696418454440504 26.63597656927774 27.40540220065702 23.262202775624736 78-79 22.699094277369497 26.7517993284663 27.52854909623637 23.020557297927834 80-81 22.60718811921622 26.63374096074527 27.551470125617488 23.20760079442102 82-83 22.5952418451699 26.599602634571042 27.579588267556904 23.22556725270216 84-85 22.615209628399903 26.633400190023572 27.39383133923291 23.35755884234361 86-87 22.72462728004218 26.66084875577243 27.373576526776645 23.240947437408742 88-89 22.528010373386998 26.577523395314874 27.508044609295524 23.386421622002608 90-91 22.413076568948163 26.526666196435126 27.51036914954964 23.54988808506706 92-93 22.53344526237593 26.717527422632713 27.349403422279885 23.399623892711475 94-95 22.52636886980785 26.47368710184976 27.440623399884323 23.559320628458064 96-97 22.400760552279365 26.555107390073058 27.46237818113823 23.581753876509346 98-99 22.319584543358662 26.60742627969005 27.476369361705217 23.596619815246072 100 22.453903830492674 26.443230330088074 27.41897417437039 23.683891665048858 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1281.0 1 1142.0 2 6489.0 3 9681.5 4 4637.5 5 1857.5 6 2844.5 7 6372.5 8 11049.5 9 12541.0 10 8933.5 11 5223.0 12 4519.0 13 4857.0 14 5486.0 15 6184.0 16 6721.5 17 7451.0 18 8520.0 19 9545.5 20 10739.0 21 12671.5 22 15650.0 23 20272.5 24 26209.0 25 33656.0 26 44459.0 27 58156.5 28 75637.0 29 95849.5 30 114583.0 31 133163.5 32 152058.5 33 169024.0 34 186530.5 35 205728.0 36 225047.5 37 245445.0 38 265732.0 39 280583.5 40 294244.0 41 307547.0 42 317109.5 43 325029.5 44 329242.0 45 333234.5 46 336441.0 47 333439.5 48 327913.5 49 329339.5 50 332885.5 51 329466.0 52 319649.0 53 313466.0 54 309009.5 55 295571.0 56 277412.0 57 254292.0 58 231908.0 59 215704.0 60 195358.0 61 168454.0 62 143914.5 63 120312.5 64 96836.5 65 77147.5 66 59524.0 67 45144.0 68 34403.5 69 26222.5 70 19916.5 71 15005.5 72 11394.0 73 8299.5 74 6073.0 75 4280.0 76 2676.5 77 1699.5 78 1081.0 79 640.0 80 325.0 81 166.5 82 87.5 83 41.5 84 21.5 85 12.0 86 13.0 87 7.5 88 5.5 89 5.0 90 2.0 91 1.5 92 0.5 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09488103644835218 2 0.0 3 0.0 4 3.109494312268479E-5 5 0.008561474339779211 6 1.1401479144984422E-4 7 0.0 8 9.328482936805436E-5 9 0.0 10-11 0.0 12-13 0.01332936561859088 14-15 2.5912452602237324E-5 16-17 0.0 18-19 1.0364981040894928E-5 20-21 2.5912452602237324E-5 22-23 0.0 24-25 5.182490520447465E-5 26-27 2.0729962081789856E-5 28-29 1.1919728197029168E-4 30-31 5.182490520447464E-6 32-33 3.627743364313225E-5 34-35 2.0729962081789856E-5 36-37 2.0729962081789856E-5 38-39 1.2437977249073916E-4 40-41 1.5547471561342396E-5 42-43 1.8656965873610872E-4 44-45 1.1401479144984422E-4 46-47 4.145992416357971E-5 48-49 2.746719975837156E-4 50-51 1.8656965873610872E-4 52-53 2.1248211133834604E-4 54-55 2.021171302974511E-4 56-57 2.072996208178986E-4 58-59 5.182490520447465E-5 60-61 7.773735780671197E-5 62-63 3.1613192174729534E-4 64-65 1.3992724405208155E-4 66-67 5.337965236060888E-4 68-69 5.441615046469837E-4 70-71 1.7102218717476633E-4 72-73 3.783218079926649E-4 74-75 3.5240935539042757E-4 76-77 2.5912452602237324E-4 78-79 0.0010883230092939675 80-81 9.328482936805435E-4 82-83 7.929210496284621E-4 84-85 0.001248980215427839 86-87 8.084685211898044E-4 88-89 0.0010986879903348624 90-91 0.0014666448172866325 92-93 9.535782557623335E-4 94-95 0.0013578125163572357 96-97 0.0015029222509297647 98-99 0.0017983242105952702 100 5.389790141265363E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9647871.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.52586613479239 #Duplication Level Percentage of deduplicated Percentage of total 1 73.02737526218533 37.6279876193461 2 14.928438472453829 15.38401444666281 3 4.859642119250148 7.511918078984459 4 2.1928596764250368 4.5195597655944235 5 1.264564402936005 3.257888807225213 6 0.803477445126371 2.4839922767943836 7 0.5475189996876338 1.9747973478912317 8 0.3819061529578592 1.5742436250688157 9 0.2950720353071459 1.3683457972221058 >10 1.5414579880280717 14.387124759418304 >50 0.10633281257311307 3.7527221907581563 >100 0.04899134717402709 4.369584656300569 >500 0.0018184649633854206 0.6010985813241119 >1k 4.2374975536721676E-4 0.32296741124759015 >5k 4.035705889238076E-5 0.12519222848985156 >10k+ 8.071411778476152E-5 0.7385624076718385 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23833 0.24702859314764883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20448 0.2119431323242195 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14638 0.15172259247661996 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12116 0.12558211029148295 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.036498104089493E-5 0.0 0.0 0.0 3 0.0 1.036498104089493E-5 0.0 1.036498104089493E-5 0.0 4 0.0 1.036498104089493E-5 0.0 1.036498104089493E-5 0.0 5 0.0 1.036498104089493E-5 0.0 1.036498104089493E-5 0.0 6 0.0 1.036498104089493E-5 0.0 1.036498104089493E-5 0.0 7 0.0 1.036498104089493E-5 0.0 1.036498104089493E-5 0.0 8 0.0 1.036498104089493E-5 0.0 1.036498104089493E-5 0.0 9 0.0 1.036498104089493E-5 0.0 1.036498104089493E-5 0.0 10-11 0.0 1.036498104089493E-5 0.0 2.0729962081789856E-5 0.0 12-13 0.0 1.036498104089493E-5 0.0 4.664241468402718E-5 0.0 14-15 1.036498104089493E-5 2.5912452602237324E-5 1.036498104089493E-5 6.737237676581704E-5 0.0 16-17 1.036498104089493E-5 4.145992416357972E-5 3.1094943122684784E-5 7.773735780671197E-5 0.0 18-19 1.036498104089493E-5 5.182490520447465E-5 3.1094943122684784E-5 1.1919728197029168E-4 0.0 20-21 1.036498104089493E-5 6.218988624536957E-5 3.1094943122684784E-5 1.7102218717476633E-4 0.0 22-23 1.036498104089493E-5 6.218988624536957E-5 3.1094943122684784E-5 2.7985448810416305E-4 0.0 24-25 1.036498104089493E-5 7.25548672862645E-5 4.145992416357972E-5 7.670085970262247E-4 0.0 26-27 1.036498104089493E-5 7.25548672862645E-5 4.145992416357972E-5 0.0014044549310412628 0.0 28-29 1.036498104089493E-5 7.773735780671197E-5 4.145992416357972E-5 0.0031250417838298208 0.0 30-31 1.036498104089493E-5 8.291984832715944E-5 4.145992416357972E-5 0.007530158726210165 0.0 32-33 1.036498104089493E-5 8.291984832715944E-5 4.145992416357972E-5 0.017257693433090054 0.0 34-35 1.036498104089493E-5 8.291984832715944E-5 4.145992416357972E-5 0.031006840783837183 0.0 36-37 1.036498104089493E-5 9.328482936805436E-5 4.145992416357972E-5 0.052742206026593845 0.0 38-39 1.036498104089493E-5 1.036498104089493E-4 4.145992416357972E-5 0.08653722671043176 1.036498104089493E-5 40-41 1.036498104089493E-5 1.036498104089493E-4 4.145992416357972E-5 0.12723014227698526 1.036498104089493E-5 42-43 1.036498104089493E-5 1.45109734572529E-4 4.145992416357972E-5 0.17112583698517528 1.036498104089493E-5 44-45 1.036498104089493E-5 1.45109734572529E-4 4.145992416357972E-5 0.2212871627325863 1.036498104089493E-5 46-47 2.072996208178986E-5 1.45109734572529E-4 4.145992416357972E-5 0.2838398233143872 1.036498104089493E-5 48-49 2.072996208178986E-5 1.45109734572529E-4 6.218988624536957E-5 0.3530364367434017 1.036498104089493E-5 50-51 2.072996208178986E-5 1.5547471561342394E-4 6.218988624536957E-5 0.4260473631954656 1.036498104089493E-5 52-53 2.072996208178986E-5 1.5547471561342394E-4 6.218988624536957E-5 0.5016495349077532 1.036498104089493E-5 54-55 2.5912452602237324E-5 1.5547471561342394E-4 6.218988624536957E-5 0.5855592389243182 1.036498104089493E-5 56-57 3.1094943122684784E-5 1.606572061338714E-4 6.218988624536957E-5 0.6836482370048274 1.036498104089493E-5 58-59 3.1094943122684784E-5 1.8656965873610872E-4 6.218988624536957E-5 0.7914233098680528 1.036498104089493E-5 60-61 3.1094943122684784E-5 1.9693463977700365E-4 6.218988624536957E-5 0.9061066425950347 1.036498104089493E-5 62-63 4.145992416357972E-5 2.0211713029745114E-4 6.218988624536957E-5 1.0236403451082627 1.036498104089493E-5 64-65 4.145992416357972E-5 2.072996208178986E-4 6.218988624536957E-5 1.1522542123542077 1.036498104089493E-5 66-67 4.664241468402718E-5 2.3839456394058337E-4 6.218988624536957E-5 1.2985351897843576 1.036498104089493E-5 68-69 5.182490520447465E-5 2.3839456394058337E-4 6.218988624536957E-5 1.4589488188637678 1.036498104089493E-5 70-71 5.182490520447465E-5 2.3839456394058337E-4 6.218988624536957E-5 1.630546262486304 1.036498104089493E-5 72-73 6.737237676581704E-5 2.487595449814783E-4 6.218988624536957E-5 1.8157011013103306 1.5547471561342396E-5 74-75 7.25548672862645E-5 2.539420355019258E-4 6.218988624536957E-5 2.0029237538520155 2.072996208178986E-5 76-77 7.25548672862645E-5 2.643070165428207E-4 6.218988624536957E-5 2.203563874351139 2.072996208178986E-5 78-79 7.25548672862645E-5 2.746719975837156E-4 6.218988624536957E-5 2.4255351258324245 2.072996208178986E-5 80-81 7.25548672862645E-5 2.798544881041631E-4 6.218988624536957E-5 2.667754367777098 2.5912452602237324E-5 82-83 8.291984832715944E-5 2.798544881041631E-4 6.218988624536957E-5 2.9228987410797678 3.1094943122684784E-5 84-85 8.291984832715944E-5 2.90219469145058E-4 6.218988624536957E-5 3.177799537327976 3.1094943122684784E-5 86-87 8.291984832715944E-5 3.005844501859529E-4 6.218988624536957E-5 3.4456099174626194 3.1094943122684784E-5 88 8.291984832715944E-5 3.213144122677428E-4 6.218988624536957E-5 3.662507510724387 3.1094943122684784E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13650 0.0 46.56926 1 TATAACG 1405 0.0 39.47568 2 GTATCAA 23755 0.0 39.03986 1 ATAACGC 1680 0.0 34.692562 3 TAACGCA 1705 0.0 34.45955 4 TACCTGG 9165 0.0 32.155777 2 ATCAACG 28350 0.0 31.981815 3 TCAACGC 28415 0.0 31.825949 4 CAACGCA 29630 0.0 30.60022 5 GTACCTG 10060 0.0 30.448063 1 TATCACG 1670 0.0 29.83413 2 TATCAAC 30745 0.0 29.536333 2 AACGCAG 31365 0.0 29.362576 6 ACGCAGA 36275 0.0 25.388206 7 CGCAGAG 37220 0.0 24.743608 8 ACCTGGG 11890 0.0 24.628057 3 GTATAAC 3405 0.0 24.596851 1 GTACATG 30230 0.0 22.366356 1 GCAGAGT 42135 0.0 21.80151 9 GTGGTAT 7195 0.0 21.384228 1 >>END_MODULE