FastQCFastQC Report
Wed 25 May 2016
SRR2049481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5610783
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG7500.044.494892
TACCTGG59650.039.870632
GTACCTG64600.037.9261671
ATAACGC9450.034.816033
GGTATCA60500.032.2571871
TATCACG8750.031.6925352
TAACGCA10400.031.635724
GTATCAA86700.029.3977381
ACCTGGG81250.029.0397833
TCAACGC88100.027.475424
ATCAACG89200.027.0839043
GTATAAC18150.025.3911971
CAACGCA98050.025.2149965
GTACATG187400.023.8389841
AACGCAG106050.023.4013086
TATCAAC106200.023.0139872
TACATGG193850.022.282442
ACATGGG188050.021.9949173
CCTGGGG80050.020.9613824
ACGCAGA120950.020.596197
Produced by FastQC (version 0.11.5)