##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049481_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5610783 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.128560309675137 31.0 31.0 34.0 26.0 34.0 2 30.22904485880135 31.0 31.0 34.0 26.0 34.0 3 30.469966313079652 31.0 31.0 34.0 27.0 34.0 4 34.064679386103506 37.0 35.0 37.0 30.0 37.0 5 34.034183286004826 37.0 35.0 37.0 30.0 37.0 6 33.959944806277484 37.0 35.0 37.0 30.0 37.0 7 33.97057416050487 37.0 35.0 37.0 30.0 37.0 8 33.94273294832468 37.0 35.0 37.0 30.0 37.0 9 35.482620518383975 39.0 35.0 39.0 30.0 39.0 10-11 35.41264427799115 39.0 35.0 39.0 30.0 39.0 12-13 35.325614089869454 39.0 35.0 39.0 29.0 39.0 14-15 36.46866702918291 40.0 37.0 41.0 29.0 41.0 16-17 36.42500164415555 40.0 37.0 41.0 29.0 41.0 18-19 36.398865541583056 40.0 37.0 41.0 29.0 41.0 20-21 36.44020362220389 40.0 37.0 41.0 29.0 41.0 22-23 36.36870451058257 40.0 37.0 41.0 29.0 41.0 24-25 36.33837068729979 40.0 37.0 41.0 29.0 41.0 26-27 36.30868739710661 40.0 36.5 41.0 29.0 41.0 28-29 36.261342668215825 40.0 36.0 41.0 29.0 41.0 30-31 36.184877137469044 40.0 36.0 41.0 29.0 41.0 32-33 36.13544767281144 40.0 36.0 41.0 28.0 41.0 34-35 36.04871788839454 40.0 36.0 41.0 28.0 41.0 36-37 35.93277462343491 40.0 36.0 41.0 28.0 41.0 38-39 35.81012703574528 40.0 35.5 41.0 27.0 41.0 40-41 35.68244316345864 40.0 35.0 41.0 27.0 41.0 42-43 35.54850918312114 39.5 35.0 41.0 26.5 41.0 44-45 35.361577697800826 39.0 35.0 41.0 26.0 41.0 46-47 35.209303585613625 39.0 35.0 41.0 25.5 41.0 48-49 35.066704504522804 39.0 35.0 41.0 25.0 41.0 50-51 34.90687173251933 39.0 35.0 41.0 24.5 41.0 52-53 34.74131756654998 39.0 34.0 40.0 24.0 41.0 54-55 34.55846064622353 38.5 34.0 40.0 24.0 41.0 56-57 34.36172527078662 38.0 34.0 40.0 23.5 41.0 58-59 34.13245156692034 38.0 34.0 40.0 23.0 41.0 60-61 33.87557993242655 37.5 33.5 40.0 22.0 41.0 62-63 33.583320278114485 37.0 33.0 40.0 22.0 41.0 64-65 33.30169577044772 36.5 33.0 40.0 21.0 41.0 66-67 33.026263090196146 36.0 33.0 39.0 20.5 41.0 68-69 32.70961290073061 35.5 33.0 39.0 20.0 41.0 70-71 32.385354682225284 35.0 32.0 39.0 18.5 40.0 72-73 32.0371707478261 35.0 32.0 38.0 17.5 40.0 74-75 31.728531026774696 35.0 32.0 37.0 16.0 39.5 76-77 31.42373497602741 35.0 32.0 37.0 13.0 39.0 78-79 31.113504122330163 35.0 31.5 36.0 9.5 39.0 80-81 30.811909852867238 35.0 31.0 36.0 7.0 38.0 82-83 30.54370397144213 35.0 31.0 35.5 4.5 37.0 84-85 30.292004520581173 35.0 31.0 35.0 2.0 37.0 86-87 30.036534383881893 35.0 31.0 35.0 2.0 36.0 88-89 29.827180092332924 34.0 31.0 35.0 2.0 36.0 90-91 29.634781722978772 34.0 31.0 35.0 2.0 36.0 92-93 29.4196622824301 34.0 30.5 35.0 2.0 35.5 94-95 29.205278033386783 34.0 30.0 35.0 2.0 35.0 96-97 28.97322726614093 34.0 30.0 35.0 2.0 35.0 98-99 28.763765859417482 34.0 30.0 35.0 2.0 35.0 100 28.611072287058686 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29164502620413657 1101 2 0.2879713172135503 1101 3 0.3443231325086771 1101 4 0.4170793082294182 1101 5 0.3733499140044998 1101 6 0.374276334440232 1101 7 0.4178268128057283 1101 8 0.45188651258760615 1101 9 0.569258196486345 1101 10-11 0.5933196882035432 1101 12-13 0.5681736531242336 1101 14-15 0.6277447473444653 1101 16-17 0.6294643571319583 1101 18-19 0.6421199543591456 1101 20-21 0.5546566262212735 1101 22-23 0.5420397658229987 1101 24-25 0.6236610693473352 1101 26-27 0.6246448423568225 1101 28-29 0.5864254882865296 1101 30-31 0.5558131550151302 1101 32-33 0.5847414261624877 1101 34-35 0.5962832554002233 1101 36-37 0.5597349314306612 1101 38-39 0.5420933585073442 1101 40-41 0.619289206524563 1101 42-43 0.5001589949645009 1101 44-45 0.4982110400177078 1101 46-47 0.4720320380408083 1101 48-49 0.41014114586972994 1101 50-51 0.39638424056726507 1101 52-53 0.4477075638728607 1101 54-55 0.4267197982591924 1101 56-57 0.39899337173716276 1101 58-59 0.39368753858662586 1101 60-61 0.4351072231871882 1101 62-63 0.38997604042972966 1101 64-65 0.4083234817043362 1101 66-67 0.35112791558749734 1101 68-69 0.3358184523637604 1101 70-71 0.33731653004272033 1101 72-73 0.3867472533363525 1101 74-75 0.34833124259886716 1101 76-77 0.33520575369084327 1101 78-79 0.308891385090476 1101 80-81 0.38933965057055886 1101 82-83 0.31286171647535355 1101 84-85 0.30266209892627316 1101 86-87 0.252253193374365 1101 88-89 0.30771806708560945 1101 90-91 0.31691105726968516 1101 92-93 0.19995076170072323 1101 94-95 0.245538863837222 1101 96-97 0.25933537747068414 1101 98-99 0.3094074170452785 1101 100 0.3125765241916767 1102 1 0.03351246771572747 1102 2 0.08790195421498126 1102 3 0.18535073260107993 1102 4 0.23550897411818994 1102 5 0.2168138549088141 1102 6 0.18844319770186502 1102 7 0.19984102715856267 1102 8 0.35635042496137004 1102 9 0.42011917505989516 1102 10-11 0.3387611341444341 1102 12-13 0.3653951603782062 1102 14-15 0.4930721370163482 1102 16-17 0.4648176743306962 1102 18-19 0.4609757762646609 1102 20-21 0.4336858387502929 1102 22-23 0.43152541970808045 1102 24-25 0.46220001493772145 1102 26-27 0.32896216749134766 1102 28-29 0.38747707705505974 1102 30-31 0.33767401762577265 1102 32-33 0.36692379440116696 1102 34-35 0.3599409019115356 1102 36-37 0.40160929083530306 1102 38-39 0.41498728956707964 1102 40-41 0.4590066481742099 1102 42-43 0.35390224234927103 1102 44-45 0.3710629115148478 1102 46-47 0.3543299857120843 1102 48-49 0.2627822854599273 1102 50-51 0.2661115482674745 1102 52-53 0.21772954160491054 1102 54-55 0.3376557447051951 1102 56-57 0.25648337913976604 1102 58-59 0.2859618319721662 1102 60-61 0.297629990111254 1102 62-63 0.2730558787443016 1102 64-65 0.29797317095135867 1102 66-67 0.3298813020572027 1102 68-69 0.343444539784457 1102 70-71 0.2552703875427156 1102 72-73 0.24740601622073655 1102 74-75 0.2407304568734716 1102 76-77 0.17649690096588344 1102 78-79 0.23154411023414667 1102 80-81 0.2163082359734041 1102 82-83 0.1535165326327288 1102 84-85 0.17713451371271205 1102 86-87 0.15940991479004651 1102 88-89 0.17826668668217494 1102 90-91 0.22056632009900312 1102 92-93 0.20063168331746795 1102 94-95 0.17496826344079963 1102 96-97 0.1428169904472405 1102 98-99 0.25073846875216077 1102 100 0.17056733489818043 1103 1 0.17078163196374163 1103 2 0.16818239395161427 1103 3 0.2274204788098011 1103 4 0.23170410910486794 1103 5 0.21176772116177744 1103 6 0.2551477709925365 1103 7 0.28629336799156846 1103 8 0.2747267991383495 1103 9 0.3431169407464836 1103 10-11 0.40053136358837094 1103 12-13 0.436346339516696 1103 14-15 0.45983531464055005 1103 16-17 0.46200273634959643 1103 18-19 0.3576139511052574 1103 20-21 0.38354591580070974 1103 22-23 0.3852559436953982 1103 24-25 0.3945368024255629 1103 26-27 0.4100174537581225 1103 28-29 0.39393494799933393 1103 30-31 0.4055596222636453 1103 32-33 0.4592388325923409 1103 34-35 0.46222461517711366 1103 36-37 0.40905809091250234 1103 38-39 0.4368018341308755 1103 40-41 0.4322573515004393 1103 42-43 0.40803568504767185 1103 44-45 0.39706326781732315 1103 46-47 0.32810252369912263 1103 48-49 0.380052080660235 1103 50-51 0.35993107364289045 1103 52-53 0.3788414280038097 1103 54-55 0.38388079425350696 1103 56-57 0.3359488512034261 1103 58-59 0.36263381843267695 1103 60-61 0.34568428170028653 1103 62-63 0.3808579749905121 1103 64-65 0.3305075578512984 1103 66-67 0.34067518850063294 1103 68-69 0.3072257626442294 1103 70-71 0.31741699858661576 1103 72-73 0.2951758225554997 1103 74-75 0.3147162196205642 1103 76-77 0.3455456847125511 1103 78-79 0.28260886078530234 1103 80-81 0.29419328388866717 1103 82-83 0.3108510932301307 1103 84-85 0.3187900954136076 1103 86-87 0.36056344404025253 1103 88-89 0.3374980012156037 1103 90-91 0.3623639031805723 1103 92-93 0.34031409162525605 1103 94-95 0.4005539356276806 1103 96-97 0.3757812414326409 1103 98-99 0.3627042934068072 1103 100 0.3303851752598277 1104 1 0.11651851324263873 1104 2 0.06853446973898158 1104 3 0.1195543690584735 1104 4 0.2165202067154013 1104 5 0.18531412240741219 1104 6 0.2162339288314925 1104 7 0.23303188989866896 1104 8 0.24125990027150834 1104 9 0.3187654212019666 1104 10-11 0.360386300928198 1104 12-13 0.34085405378367284 1104 14-15 0.40984785892562314 1104 16-17 0.3643815607894112 1104 18-19 0.3522319268279901 1104 20-21 0.3094312750132602 1104 22-23 0.4002665677897923 1104 24-25 0.413404740140777 1104 26-27 0.36316617018450614 1104 28-29 0.40267830749001376 1104 30-31 0.3373792857367235 1104 32-33 0.3699483304390583 1104 34-35 0.39639614116310184 1104 36-37 0.36313745495110794 1104 38-39 0.3392042628658629 1104 40-41 0.338291606549312 1104 42-43 0.3645701479671004 1104 44-45 0.3709331761597454 1104 46-47 0.3100388540670309 1104 48-49 0.2221415867641383 1104 50-51 0.30193908872774244 1104 52-53 0.2656466270011322 1104 54-55 0.2565761871648675 1104 56-57 0.29792359979533956 1104 58-59 0.3640689321994657 1104 60-61 0.347423931224057 1104 62-63 0.3670541096790956 1104 64-65 0.3503626248850509 1104 66-67 0.3498833998556208 1104 68-69 0.3013870067836848 1104 70-71 0.3156450408639486 1104 72-73 0.24775369115104695 1104 74-75 0.35519779479656677 1104 76-77 0.3315636847889287 1104 78-79 0.32400902924330666 1104 80-81 0.38589484662846374 1104 82-83 0.4090081352862711 1104 84-85 0.3697886594136044 1104 86-87 0.3610945406816839 1104 88-89 0.3292667886422116 1104 90-91 0.3610087241056803 1104 92-93 0.33940264591981517 1104 94-95 0.35621255382532624 1104 96-97 0.35480229003687924 1104 98-99 0.4039287337955102 1104 100 0.37637700598331136 1105 1 0.22419694513131105 1105 2 0.17709603725792178 1105 3 0.28834296432793494 1105 4 0.23266953138419666 1105 5 0.2539993708289572 1105 6 0.2611609705953839 1105 7 0.3511094708698863 1105 8 0.3446235279093415 1105 9 0.4027515358117668 1105 10-11 0.47322811430561273 1105 12-13 0.37774760708916943 1105 14-15 0.49538617417221076 1105 16-17 0.440062546939366 1105 18-19 0.48111416437317445 1105 20-21 0.4690295384173737 1105 22-23 0.45033462274399483 1105 24-25 0.47027728457034357 1105 26-27 0.4946692429963164 1105 28-29 0.5184114504762505 1105 30-31 0.48889672539505824 1105 32-33 0.5064609869699908 1105 34-35 0.49954653646444314 1105 36-37 0.4333607579118919 1105 38-39 0.4949552348250137 1105 40-41 0.5444861162986641 1105 42-43 0.5284249476716312 1105 44-45 0.47030170135118254 1105 46-47 0.3490920868450047 1105 48-49 0.43287144715717574 1105 50-51 0.47859465367676535 1105 52-53 0.45274600078657556 1105 54-55 0.4387947171974176 1105 56-57 0.40143148269946494 1105 58-59 0.434385075474502 1105 60-61 0.417774891363365 1105 62-63 0.3918018934920795 1105 64-65 0.40211750941028157 1105 66-67 0.40331415334593856 1105 68-69 0.4448608426506695 1105 70-71 0.4553128849050765 1105 72-73 0.3687812538965005 1105 74-75 0.3644187241469723 1105 76-77 0.4498350777750595 1105 78-79 0.3960250234848317 1105 80-81 0.41325936508622263 1105 82-83 0.40717591177541124 1105 84-85 0.3485432886803643 1105 86-87 0.3857506507968651 1105 88-89 0.3248547710119105 1105 90-91 0.3641954940884595 1105 92-93 0.34516240592376235 1105 94-95 0.40005448162895263 1105 96-97 0.36755632076653555 1105 98-99 0.35833185859510763 1105 100 0.3230415378117506 1106 1 0.10102543681719922 1106 2 0.04252501035089651 1106 3 0.05808745533780524 1106 4 0.1250798807844049 1106 5 0.11266119765480198 1106 6 0.1856218623540542 1106 7 0.227779429680929 1106 8 0.20567542837109443 1106 9 0.2242097996497634 1106 10-11 0.17517952623604316 1106 12-13 0.14590842598980203 1106 14-15 0.19958844750391336 1106 16-17 0.2434699743143156 1106 18-19 0.15905771742527008 1106 20-21 0.18594261823854197 1106 22-23 0.2090901894437991 1106 24-25 0.2613391705443462 1106 26-27 0.23353076097824754 1106 28-29 0.21578117778729933 1106 30-31 0.1746595746945374 1106 32-33 0.14565311696559036 1106 34-35 0.19129861379118296 1106 36-37 0.1768008858453456 1106 38-39 0.2302602347190046 1106 40-41 0.2504857204777906 1106 42-43 0.24122419688000463 1106 44-45 0.15853487243612818 1106 46-47 0.14475534622064856 1106 48-49 0.1885915122146926 1106 50-51 0.2245191473331687 1106 52-53 0.21403358715252807 1106 54-55 0.22628047811056007 1106 56-57 0.14994341503550146 1106 58-59 0.13982108144412564 1106 60-61 0.0948371191640689 1106 62-63 0.130602497599547 1106 64-65 0.10634957497828879 1106 66-67 0.15034687728828544 1106 68-69 0.21181603724067344 1106 70-71 0.18378647729178965 1106 72-73 0.1650406988705555 1106 74-75 0.14265983142009375 1106 76-77 0.0788442418533144 1106 78-79 0.05647184839940422 1106 80-81 0.15895745819902984 1106 82-83 0.1364094993381002 1106 84-85 0.14125696667782606 1106 86-87 0.13545595649418374 1106 88-89 0.14171369986443594 1106 90-91 0.1072909797056596 1106 92-93 0.07537122904793492 1106 94-95 0.12460410925667631 1106 96-97 0.17569686867507528 1106 98-99 0.18397636795072358 1106 100 0.13933360971589437 1107 1 -0.04653603872246492 1107 2 -0.036752551814881684 1107 3 0.02002706300806878 1107 4 -0.022649893267036703 1107 5 -0.006790264970376825 1107 6 -0.01657134014934769 1107 7 0.015088804240150466 1107 8 0.0555801652512784 1107 9 0.11767350719875225 1107 10-11 0.04630392048377985 1107 12-13 0.06667780014221592 1107 14-15 0.1072287304422801 1107 16-17 0.0811379435449524 1107 18-19 0.09495291698964792 1107 20-21 0.14903245913989593 1107 22-23 0.09899671361203843 1107 24-25 0.1263883593123083 1107 26-27 0.11127438984654958 1107 28-29 0.09057948266463 1107 30-31 0.10221246725018318 1107 32-33 0.11341052729191148 1107 34-35 0.11266947706130992 1107 36-37 0.1316304816014764 1107 38-39 0.12999014081636062 1107 40-41 0.18563298667734074 1107 42-43 0.14965792900753883 1107 44-45 0.11274345895471782 1107 46-47 0.10801024450695706 1107 48-49 0.13675738454963948 1107 50-51 0.16147488894600315 1107 52-53 0.12659720843819855 1107 54-55 0.10862650707996835 1107 56-57 0.10123975572994937 1107 58-59 0.10114765598320474 1107 60-61 0.1311138647126029 1107 62-63 0.10922819980201126 1107 64-65 0.14679405617625463 1107 66-67 0.13088043612223288 1107 68-69 0.134094589417316 1107 70-71 0.09354819778328505 1107 72-73 0.11737113241794361 1107 74-75 0.17190594598592313 1107 76-77 0.15032394629722745 1107 78-79 0.08922474215546572 1107 80-81 0.09313666116859665 1107 82-83 0.053068217685847685 1107 84-85 0.05190430173464833 1107 86-87 0.04615646337040502 1107 88-89 -0.009228684437839973 1107 90-91 0.10407634875229732 1107 92-93 0.0700809698011362 1107 94-95 0.015154650661571623 1107 96-97 0.09269776598704027 1107 98-99 0.09703163349804456 1107 100 0.0523401783329156 1108 1 -0.05010331760105302 1108 2 -0.01810834802304484 1108 3 -0.009533999809686833 1108 4 -0.08023978046416858 1108 5 3.174232770035701E-4 1108 6 0.05449283699843477 1108 7 -0.014486634958963407 1108 8 0.030787392698499616 1108 9 0.09296656853908303 1108 10-11 0.0409096608243118 1108 12-13 0.05641939662344697 1108 14-15 0.05108046671525557 1108 16-17 0.029951865558388135 1108 18-19 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-0.4973847759325807 2215 44-45 -0.4550726259828579 2215 46-47 -0.4492909998488699 2215 48-49 -0.4489191556960037 2215 50-51 -0.43705737422036606 2215 52-53 -0.4636927281324006 2215 54-55 -0.5065303276280773 2215 56-57 -0.44388874014778423 2215 58-59 -0.4981409074243075 2215 60-61 -0.39895103836131796 2215 62-63 -0.3722740850007682 2215 64-65 -0.36241485514035077 2215 66-67 -0.302784854914961 2215 68-69 -0.3177891350547668 2215 70-71 -0.29024664043073045 2215 72-73 -0.33487558312025456 2215 74-75 -0.3585825658740234 2215 76-77 -0.3386881223695575 2215 78-79 -0.35325398676537034 2215 80-81 -0.40657464162218915 2215 82-83 -0.3512047242189311 2215 84-85 -0.37085006485611416 2215 86-87 -0.37741834195428225 2215 88-89 -0.35067189301495816 2215 90-91 -0.29936589423282456 2215 92-93 -0.29531017819895666 2215 94-95 -0.3410563811345213 2215 96-97 -0.41194739745142783 2215 98-99 -0.4658282946189942 2215 100 -0.40834817900764975 2216 1 -0.40950767038576075 2216 2 -0.4178219269964565 2216 3 -0.5971718419859258 2216 4 -0.6744717346665254 2216 5 -0.7028295898504524 2216 6 -1.0295892042331332 2216 7 -1.0301369442696853 2216 8 -1.248014454066798 2216 9 -1.2825603501225942 2216 10-11 -0.97903459499706 2216 12-13 -0.9539808891293049 2216 14-15 -1.113854877224 2216 16-17 -1.18854224542293 2216 18-19 -1.1192383583428622 2216 20-21 -1.1150635943865979 2216 22-23 -1.150353114996122 2216 24-25 -1.1553729863089188 2216 26-27 -1.2294340976848943 2216 28-29 -1.2669145997399127 2216 30-31 -1.2379180085130699 2216 32-33 -1.3144836052504942 2216 34-35 -1.5382163793375483 2216 36-37 -1.3056142328519655 2216 38-39 -1.2125859512644226 2216 40-41 -1.380738702224626 2216 42-43 -1.3218241233970218 2216 44-45 -1.2116109589351183 2216 46-47 -1.132545865906252 2216 48-49 -1.2435767670229296 2216 50-51 -1.3154730118242455 2216 52-53 -1.5049777966018354 2216 54-55 -1.2884541355367887 2216 56-57 -1.2889251157192234 2216 58-59 -1.609924111861197 2216 60-61 -1.7416371634377725 2216 62-63 -1.5518275850546885 2216 64-65 -1.5364281660116674 2216 66-67 -1.4724644324186968 2216 68-69 -1.5441290729992616 2216 70-71 -1.4445992637249532 2216 72-73 -1.5620172473911893 2216 74-75 -1.7387477881580544 2216 76-77 -1.9711631291556273 2216 78-79 -1.7222165516237027 2216 80-81 -1.6483019806139687 2216 82-83 -1.6434968632062237 2216 84-85 -1.6121804707725147 2216 86-87 -1.6258982906345238 2216 88-89 -1.622636197522617 2216 90-91 -1.6893437111476288 2216 92-93 -1.7193008186078167 2216 94-95 -1.6496521997019151 2216 96-97 -1.651146350949027 2216 98-99 -1.6775490129778419 2216 100 -1.646498123660649 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 159492.0 3 26073.0 4 16712.0 5 13370.0 6 11494.0 7 11676.0 8 12649.0 9 14024.0 10 15629.0 11 16688.0 12 17966.0 13 19226.0 14 19890.0 15 20568.0 16 21533.0 17 22393.0 18 21737.0 19 20914.0 20 21403.0 21 22741.0 22 25314.0 23 30016.0 24 36351.0 25 43179.0 26 52030.0 27 60551.0 28 72968.0 29 89425.0 30 107710.0 31 128636.0 32 162800.0 33 212554.0 34 291078.0 35 425322.0 36 705958.0 37 1163391.0 38 1186984.0 39 309880.0 40 458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.53559312180814 18.22766494805192 12.48856282781285 23.748179102327093 2 17.296658193181557 19.868400436431124 37.41694591259363 25.417995457793687 3 18.661644565738598 25.16794506824579 29.359771260296586 26.810639105719027 4 13.469461019779411 16.99754156628181 36.75009037310725 32.78290704083153 5 13.906372741861684 35.925029460274935 33.28031583054625 16.88828196731714 6 31.025214961207343 35.906266610719776 18.396415596064372 14.672102832008516 7 28.046349357901917 31.170319326119227 21.837074887109853 18.946256428869003 8 26.342070250891968 33.88239421154554 20.372548334564456 19.40298720299804 9 25.57942089906295 17.484649399175513 20.77462535478796 36.16130434697358 10-11 24.793323830529953 26.109803458104196 27.550393512496203 21.546479198869644 12-13 25.318585431396183 23.79851466285788 27.52816815103824 23.35473175470769 14-15 22.984280338056287 25.708944498786767 24.922136982760733 26.384638180396212 16-17 22.855497564280284 28.549235834549847 25.11095699983431 23.484309601335557 18-19 22.79154743192702 27.81421911480082 26.75326314303427 22.64097031023789 20-21 23.97067166137917 27.27149569090181 26.16404921093079 22.593783436788232 22-23 23.594902447356834 27.33089911109324 26.10610213055796 22.96809631099196 24-25 23.24761802900256 27.342584917600295 26.296941985971106 23.112855067426043 26-27 23.154204387240934 27.151656894922006 26.317189174120177 23.37694954371689 28-29 23.200480286660405 27.21433380457659 26.37028490104159 23.214901007721416 30-31 23.329618854945416 27.73836572517132 25.992840144601736 22.939175275281528 32-33 22.95824647122209 27.394680340003337 26.3919521492635 23.25512103951108 34-35 23.03214602585247 26.909339000490633 26.560967468954445 23.497547504702453 36-37 23.554270997862947 27.280443931160082 26.207451650686743 22.957833420290232 38-39 23.326976531350212 27.430064036847345 26.19408853067852 23.04887090112392 40-41 23.17379975481054 27.165690944523103 26.421593071949424 23.238916228716935 42-43 22.898807041585588 27.34216337468919 26.43783471269865 23.321194871026574 44-45 23.150729497289063 27.37993309784774 26.306138401710612 23.163199003152585 46-47 23.20972191843604 27.494265794400718 26.086210151348805 23.20980213581444 48-49 23.077626518240272 27.245505352790765 26.39118547166878 23.285682657300175 50-51 23.043720333961044 27.11876023329894 26.419301912432058 23.418217520307955 52-53 23.060485527342962 27.13433913046501 26.35636847443902 23.44880686775301 54-55 23.175392156862745 27.543511586452762 26.26336007130125 23.017736185383246 56-57 22.810786557393765 27.296563479667135 26.277807706171075 23.614842256768025 58-59 22.844767045584174 26.878949830028976 26.560370014943764 23.715913109443086 60-61 23.216614866491373 27.319735969279417 26.216741154763202 23.246908009466004 62-63 22.97555710064965 27.42011602585122 26.239117284436425 23.365209589062708 64-65 23.024158940968746 27.003788786663957 26.431009668371757 23.54104260399554 66-67 22.790999586184387 27.117819267479522 26.339760354147018 23.75142079218907 68-69 23.316456370866906 27.19465204065908 26.207177273882287 23.281714314591724 70-71 23.263262501291337 26.883707174475113 26.389063320456646 23.463967003776904 72-73 22.874568281396076 27.119482204177885 26.631728978805736 23.3742205356203 74-75 22.850430254430538 27.00079184213641 26.55585749265915 23.592920410773903 76-77 22.997002015827515 27.05039461842323 26.270729788740066 23.681873577009192 78-79 23.014772733450783 27.221888148043377 26.499280797264685 23.264058321241155 80-81 22.860905203663425 26.95816779479764 26.623445265816247 23.557481735722686 82-83 22.845883888852843 26.98593441288099 26.63809152364241 23.530090174623762 84-85 22.855826079640416 27.05234708778014 26.435281111497723 23.656545721081724 86-87 23.041523079786174 27.118463543727778 26.40554710612274 23.43446627036331 88-89 22.834584963714928 26.970107932732283 26.572443667719988 23.622863435832798 90-91 22.624188216772147 26.9180829794334 26.614537729203093 23.843191074591363 92-93 22.886415316242044 27.16086506793313 26.40075156649137 23.55196804933345 94-95 22.903783119317602 26.8301054498965 26.529082324218678 23.737029106567213 96-97 22.709757767408302 26.878203166372238 26.66032591307119 23.75171315314827 98-99 22.701278803194494 26.90533583867965 26.660139505154888 23.733245852970963 100 22.91418472026459 26.827657206574223 26.478794463824716 23.779363609336468 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3196.0 1 2701.5 2 2908.5 3 3266.5 4 2631.0 5 2169.5 6 1861.5 7 1737.0 8 1744.5 9 1725.5 10 1634.0 11 1550.5 12 1576.5 13 1629.0 14 1701.0 15 1835.0 16 2005.5 17 2206.0 18 2561.5 19 3169.0 20 4002.5 21 5217.0 22 7132.5 23 10113.0 24 13928.5 25 18706.5 26 25010.0 27 33012.5 28 42688.5 29 53616.0 30 63684.0 31 73499.5 32 83672.5 33 92980.5 34 102123.0 35 112481.5 36 124158.5 37 136091.0 38 147823.0 39 156472.0 40 163656.0 41 170573.5 42 176157.0 43 181644.0 44 184193.5 45 186220.5 46 189119.5 47 189653.5 48 189019.5 49 191210.5 50 195129.0 51 194917.0 52 190660.0 53 188818.0 54 187339.0 55 181435.0 56 171932.5 57 159120.0 58 145884.5 59 135907.0 60 124583.0 61 108919.0 62 93737.0 63 78546.0 64 63959.5 65 51692.0 66 40626.0 67 30975.0 68 23667.0 69 18201.5 70 13640.5 71 10348.0 72 8170.5 73 6293.5 74 4915.0 75 3551.0 76 2290.0 77 1568.5 78 1107.0 79 785.0 80 523.5 81 342.0 82 251.0 83 169.0 84 122.0 85 107.0 86 84.5 87 68.0 88 55.0 89 41.5 90 36.5 91 35.5 92 27.5 93 21.5 94 18.5 95 14.0 96 12.5 97 11.0 98 8.5 99 10.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052969434034429774 2 0.013081953089256882 3 0.01627223865189582 4 0.0126007368312052 5 0.012226457519387224 6 0.012493799884971492 7 0.020068500243192438 8 0.012814610723672614 9 0.013224535684235159 10-11 0.015318717547978597 12-13 0.016851480443995072 14-15 0.013732486178845269 16-17 0.014962261060532904 18-19 0.012493799884971492 20-21 0.015158312128628037 22-23 0.01629897288845425 24-25 0.016628695139341513 26-27 0.009980781648479365 28-29 0.014258259497827665 30-31 0.013429498164516433 32-33 0.014935526823974478 34-35 0.012128431985339657 36-37 0.012903724845534037 38-39 0.018607028644665104 40-41 0.014890969763043767 42-43 0.011665038551660258 44-45 0.019569461160768468 46-47 0.01828621780596398 48-49 0.017805001547912296 50-51 0.012966104730837032 52-53 0.013162155798932164 54-55 0.013955271483498828 56-57 0.019551638336396185 58-59 0.007396472114498101 60-61 0.01073825168430146 62-63 0.01626332723970968 64-65 0.021449769132044494 66-67 0.01419587961252467 68-69 0.015461300142956873 70-71 0.023036000501177822 72-73 0.019275384558625774 74-75 0.019230827497695065 76-77 0.012119520573153516 78-79 0.013393852515771864 80-81 0.018927839483366227 82-83 0.013883980186009687 84-85 0.013670106293542275 86-87 0.01673563208557522 88-89 0.016040541935056125 90-91 0.016236593003151254 92-93 0.011299670652028424 94-95 0.016307884300640393 96-97 0.009160931727354275 98-99 0.006790496085840425 100 0.004758694107399983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5610783.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.93492452712715 #Duplication Level Percentage of deduplicated Percentage of total 1 77.33571143109958 46.35110027874639 2 13.44971150767004 16.122148882476747 3 3.942347524303346 7.08852903986483 4 1.7143978930911068 4.110092333275252 5 0.9608164573247763 2.879323092709108 6 0.5693397741229922 2.0474001835411895 7 0.38736368553520784 1.625162927997175 8 0.26417366935920616 1.2666583148078612 9 0.20373672124792547 1.0989850510258878 >10 1.0786953846983247 11.47678254537462 >50 0.065501299807881 2.670438792353321 >100 0.027308745021607912 2.792089675757389 >500 7.465904043240938E-4 0.3124215833029622 >1k 1.493163136063373E-4 0.1588672987672391 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.782282437228458E-5 0.0 1.782282437228458E-5 0.0 3 0.0 3.564564874456916E-5 0.0 1.782282437228458E-5 0.0 4 0.0 5.346847311685374E-5 0.0 1.782282437228458E-5 0.0 5 0.0 7.129129748913832E-5 0.0 1.782282437228458E-5 0.0 6 0.0 7.129129748913832E-5 0.0 1.782282437228458E-5 0.0 7 0.0 7.129129748913832E-5 0.0 1.782282437228458E-5 0.0 8 0.0 7.129129748913832E-5 0.0 1.782282437228458E-5 0.0 9 0.0 7.129129748913832E-5 0.0 5.346847311685374E-5 0.0 10-11 0.0 7.129129748913832E-5 8.91141218614229E-6 5.346847311685374E-5 0.0 12-13 0.0 8.020270967528062E-5 1.782282437228458E-5 7.129129748913832E-5 1.782282437228458E-5 14-15 0.0 9.802553404756519E-5 3.564564874456916E-5 8.020270967528062E-5 1.782282437228458E-5 16-17 0.0 1.0693694623370748E-4 3.564564874456916E-5 1.1584835841984978E-4 1.782282437228458E-5 18-19 0.0 1.1584835841984978E-4 3.564564874456916E-5 1.3367118279213438E-4 1.782282437228458E-5 20-21 0.0 1.2475977060599208E-4 3.564564874456916E-5 1.693168315367035E-4 1.782282437228458E-5 22-23 0.0 1.2475977060599208E-4 3.564564874456916E-5 4.2774778493482994E-4 1.782282437228458E-5 24-25 0.0 1.4258259497827665E-4 3.564564874456916E-5 9.089640429865136E-4 1.782282437228458E-5 26-27 0.0 1.6040541935056124E-4 3.564564874456916E-5 0.0015862313691333276 1.782282437228458E-5 28-29 0.0 1.6040541935056124E-4 3.564564874456916E-5 0.0029585888457992404 1.782282437228458E-5 30-31 0.0 1.7822824372284584E-4 3.564564874456916E-5 0.006861787383329564 1.782282437228458E-5 32-33 0.0 2.049624802812727E-4 3.564564874456916E-5 0.015782110981657996 1.782282437228458E-5 34-35 8.91141218614229E-6 2.2278530465355727E-4 3.564564874456916E-5 0.028712570063750463 1.782282437228458E-5 36-37 1.782282437228458E-5 2.495195412119841E-4 3.564564874456916E-5 0.0504029473248208 1.782282437228458E-5 38-39 1.782282437228458E-5 2.7625377777041097E-4 3.564564874456916E-5 0.0817354725712971 1.782282437228458E-5 40-41 1.782282437228458E-5 2.940766021426956E-4 3.564564874456916E-5 0.12318245064904487 1.782282437228458E-5 42-43 1.782282437228458E-5 3.029880143288379E-4 3.564564874456916E-5 0.1684880702033923 1.782282437228458E-5 44-45 1.782282437228458E-5 3.029880143288379E-4 3.564564874456916E-5 0.218008787721785 1.782282437228458E-5 46-47 1.782282437228458E-5 3.029880143288379E-4 3.564564874456916E-5 0.2776796037201938 1.782282437228458E-5 48-49 1.782282437228458E-5 3.029880143288379E-4 3.564564874456916E-5 0.3460390466000913 1.782282437228458E-5 50-51 1.782282437228458E-5 3.029880143288379E-4 3.564564874456916E-5 0.4200394133938169 1.782282437228458E-5 52-53 1.782282437228458E-5 3.029880143288379E-4 3.564564874456916E-5 0.4944853507968496 1.782282437228458E-5 54-55 1.782282437228458E-5 3.2972225088726475E-4 3.564564874456916E-5 0.5797853882426036 1.782282437228458E-5 56-57 1.782282437228458E-5 3.38633663073407E-4 3.564564874456916E-5 0.6779980619460777 1.782282437228458E-5 58-59 1.782282437228458E-5 3.38633663073407E-4 3.564564874456916E-5 0.785487515735326 1.782282437228458E-5 60-61 1.782282437228458E-5 3.564564874456916E-4 3.564564874456916E-5 0.8984040195459351 1.782282437228458E-5 62-63 1.782282437228458E-5 3.564564874456916E-4 3.564564874456916E-5 1.0145018975069968 1.782282437228458E-5 64-65 1.782282437228458E-5 3.564564874456916E-4 3.564564874456916E-5 1.1453927196970548 1.782282437228458E-5 66-67 1.782282437228458E-5 3.564564874456916E-4 3.564564874456916E-5 1.2937320869475792 1.782282437228458E-5 68-69 1.782282437228458E-5 3.564564874456916E-4 3.564564874456916E-5 1.4518650961906743 1.782282437228458E-5 70-71 1.782282437228458E-5 3.742793118179762E-4 3.564564874456916E-5 1.6242207192828522 1.782282437228458E-5 72-73 1.782282437228458E-5 3.921021361902608E-4 3.564564874456916E-5 1.8176963892561875 1.782282437228458E-5 74-75 1.782282437228458E-5 3.921021361902608E-4 3.564564874456916E-5 2.009870993050346 1.782282437228458E-5 76-77 1.782282437228458E-5 3.921021361902608E-4 5.346847311685374E-5 2.209905462392682 1.782282437228458E-5 78-79 1.782282437228458E-5 3.921021361902608E-4 5.346847311685374E-5 2.432173905139443 1.782282437228458E-5 80-81 1.782282437228458E-5 3.921021361902608E-4 5.346847311685374E-5 2.672710742867796 1.782282437228458E-5 82-83 1.782282437228458E-5 4.0992496056254534E-4 5.346847311685374E-5 2.9281563731835645 1.782282437228458E-5 84-85 1.782282437228458E-5 4.2774778493482994E-4 5.346847311685374E-5 3.1803136924026467 1.782282437228458E-5 86-87 1.782282437228458E-5 4.2774778493482994E-4 5.346847311685374E-5 3.448297679664318 1.782282437228458E-5 88 1.782282437228458E-5 4.2774778493482994E-4 5.346847311685374E-5 3.665299477809069 1.782282437228458E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 750 0.0 44.49489 2 TACCTGG 5965 0.0 39.87063 2 GTACCTG 6460 0.0 37.926167 1 ATAACGC 945 0.0 34.81603 3 GGTATCA 6050 0.0 32.257187 1 TATCACG 875 0.0 31.692535 2 TAACGCA 1040 0.0 31.63572 4 GTATCAA 8670 0.0 29.397738 1 ACCTGGG 8125 0.0 29.039783 3 TCAACGC 8810 0.0 27.47542 4 ATCAACG 8920 0.0 27.083904 3 GTATAAC 1815 0.0 25.391197 1 CAACGCA 9805 0.0 25.214996 5 GTACATG 18740 0.0 23.838984 1 AACGCAG 10605 0.0 23.401308 6 TATCAAC 10620 0.0 23.013987 2 TACATGG 19385 0.0 22.28244 2 ACATGGG 18805 0.0 21.994917 3 CCTGGGG 8005 0.0 20.961382 4 ACGCAGA 12095 0.0 20.59619 7 >>END_MODULE