FastQCFastQC Report
Wed 25 May 2016
SRR2049481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5610783
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158270.2820818413401481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146780.2616034161363931No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143050.25495550264553096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80690.1438123698599643No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67220.11980502543049697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8950.049.889872
GGTATCA89800.047.2052231
TAACGCA10900.041.3958244
ATAACGC11100.040.2265173
TACCTGG59150.040.1280862
GTACCTG63100.038.249841
GTATCAA152700.037.7431951
ATCAACG181200.031.3862193
TCAACGC183350.031.0694474
ACCTGGG78200.030.1122193
TATCACG10050.029.931312
CAACGCA192200.029.810015
TATCAAC193550.029.2863982
GTATAAC19650.028.9710661
AACGCAG204850.028.410686
ACGCAGA232700.024.950057
CGCAGAG237300.024.5060218
GTGGTAT44150.024.2966161
GTACATG182200.022.516961
GCAGAGT262000.022.0162899
Produced by FastQC (version 0.11.5)