##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049481_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5610783 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.701514209335844 31.0 31.0 34.0 30.0 34.0 2 31.759348561510933 31.0 31.0 34.0 30.0 34.0 3 31.81591321567774 33.0 31.0 34.0 30.0 34.0 4 35.59665861253233 37.0 35.0 37.0 33.0 37.0 5 35.68401932493201 37.0 35.0 37.0 33.0 37.0 6 35.770469112778024 37.0 35.0 37.0 33.0 37.0 7 35.77086673998976 37.0 35.0 37.0 33.0 37.0 8 35.79002039465793 37.0 35.0 37.0 33.0 37.0 9 37.492976470485495 39.0 37.0 39.0 35.0 39.0 10-11 37.46595644493826 39.0 37.0 39.0 34.5 39.0 12-13 37.440455280484024 39.0 37.0 39.0 34.0 39.0 14-15 38.728844262913036 40.0 38.0 41.0 35.0 41.0 16-17 38.740770673184116 40.0 38.0 41.0 35.0 41.0 18-19 38.792346629695004 40.0 38.0 41.0 35.0 41.0 20-21 38.79411287159029 40.0 38.0 41.0 35.0 41.0 22-23 38.74953709669399 40.0 38.0 41.0 34.5 41.0 24-25 38.71968413321278 40.0 38.0 41.0 34.0 41.0 26-27 38.61207000876705 40.0 38.0 41.0 34.0 41.0 28-29 38.52484287843604 40.0 38.0 41.0 34.0 41.0 30-31 38.41888378146152 40.0 38.0 41.0 34.0 41.0 32-33 38.28613314754821 40.0 38.0 41.0 34.0 41.0 34-35 38.16758249249703 40.0 38.0 41.0 34.0 41.0 36-37 38.04892561341261 40.0 38.0 41.0 33.0 41.0 38-39 37.95766312830134 40.0 37.0 41.0 33.0 41.0 40-41 37.873089549177 40.0 37.0 41.0 33.0 41.0 42-43 37.740771386097094 40.0 37.0 41.0 33.0 41.0 44-45 37.57573221063798 40.0 37.0 41.0 33.0 41.0 46-47 37.35498663911971 40.0 37.0 41.0 32.5 41.0 48-49 37.1315668419185 40.0 36.5 41.0 32.0 41.0 50-51 36.962755465681 40.0 36.0 41.0 31.0 41.0 52-53 36.81414002288095 39.0 36.0 41.0 31.0 41.0 54-55 36.640067348888735 39.0 35.0 41.0 31.0 41.0 56-57 36.457818008645134 39.0 35.0 41.0 31.0 41.0 58-59 36.24087796658684 39.0 35.0 41.0 31.0 41.0 60-61 36.00824697729354 38.0 35.0 40.0 30.0 41.0 62-63 35.74443103573958 38.0 35.0 40.0 30.0 41.0 64-65 35.4832785370598 37.0 35.0 40.0 30.0 41.0 66-67 35.18400043273817 37.0 34.5 40.0 29.0 41.0 68-69 34.86161762092742 36.0 34.0 39.0 29.0 41.0 70-71 34.54201864516949 36.0 34.0 39.0 29.0 41.0 72-73 34.212653741910884 35.0 34.0 39.0 29.0 40.5 74-75 33.90241959099113 35.0 34.0 37.5 29.0 40.0 76-77 33.603643912088565 35.0 34.0 37.0 29.0 39.0 78-79 33.31739687669261 35.0 34.0 36.5 28.0 39.0 80-81 33.044470352890144 35.0 33.0 36.0 28.0 39.0 82-83 32.8057907425755 35.0 33.0 36.0 27.5 37.0 84-85 32.56923534558368 35.0 33.0 35.0 27.0 37.0 86-87 32.36384556308808 35.0 33.0 35.0 27.0 36.5 88-89 32.17962653340897 35.0 33.0 35.0 27.0 36.0 90-91 32.013627331515046 35.0 33.0 35.0 26.5 36.0 92-93 31.85749350848179 35.0 33.0 35.0 26.0 36.0 94-95 31.70462634181361 35.0 33.0 35.0 26.0 35.5 96-97 31.54360086640314 35.0 33.0 35.0 25.0 35.0 98-99 31.399129854068498 35.0 33.0 35.0 25.0 35.0 100 31.28525448230666 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16214486409245765 1101 2 0.14501398775244922 1101 3 0.3070314186150469 1101 4 0.20570713263974483 1101 5 0.19915467659661346 1101 6 0.141996199545126 1101 7 0.1352262625214209 1101 8 0.11589393386304181 1101 9 0.2579518233212781 1101 10-11 0.2418133411849297 1101 12-13 0.21121372668466876 1101 14-15 0.263256324546866 1101 16-17 0.2685870745536576 1101 18-19 0.2909227715781242 1101 20-21 0.28150046872291057 1101 22-23 0.2589816397155573 1101 24-25 0.2843200502777208 1101 26-27 0.2809339263428541 1101 28-29 0.34957049935582063 1101 30-31 0.29027639719195264 1101 32-33 0.28430615663461367 1101 34-35 0.2876517560285876 1101 36-37 0.39402444100412737 1101 38-39 0.3611811596741106 1101 40-41 0.3779581585304257 1101 42-43 0.31654149227053807 1101 44-45 0.28090868606008 1101 46-47 0.24232136406517668 1101 48-49 0.27417271770258367 1101 50-51 0.27864136129597483 1101 52-53 0.2575090575040946 1101 54-55 0.219758123262892 1101 56-57 0.21846506821575673 1101 58-59 0.2383900582037839 1101 60-61 0.17903265360982346 1101 62-63 0.20264060411674478 1101 64-65 0.11948081384148423 1101 66-67 0.1633958900404835 1101 68-69 0.22516667132480706 1101 70-71 0.27847774223879185 1101 72-73 0.19541130044034816 1101 74-75 0.2235663146211806 1101 76-77 0.20492685841853842 1101 78-79 0.24077268471944535 1101 80-81 0.2594358170485691 1101 82-83 0.16289323535339406 1101 84-85 0.24168991957624542 1101 86-87 0.13437829418720781 1101 88-89 0.17182071996255388 1101 90-91 0.16376528664476098 1101 92-93 0.19765942749067378 1101 94-95 0.18410909589870883 1101 96-97 0.19214932536279505 1101 98-99 0.24923584655095965 1101 100 0.2787442277197414 1102 1 0.1791839107669162 1102 2 0.13581299059415386 1102 3 0.3171879133201756 1102 4 0.1711850976931899 1102 5 0.1690623020167905 1102 6 0.1274908196945077 1102 7 0.14370362659249025 1102 8 0.12148948162365514 1102 9 0.12648330955160247 1102 10-11 0.21938183522863852 1102 12-13 0.15452218372455917 1102 14-15 0.2575618785259124 1102 16-17 0.21224276073962045 1102 18-19 0.236276869532432 1102 20-21 0.23145025647704642 1102 22-23 0.20860258979536894 1102 24-25 0.19394597633203858 1102 26-27 0.19523721475027145 1102 28-29 0.1925344882712423 1102 30-31 0.21435357173422886 1102 32-33 0.2518268405212609 1102 34-35 0.2928300367281551 1102 36-37 0.3310285270093374 1102 38-39 0.28716162912754584 1102 40-41 0.333728302560651 1102 42-43 0.29842618403161936 1102 44-45 0.32162236566437485 1102 46-47 0.30384400459581684 1102 48-49 0.2755715432867021 1102 50-51 0.34032351985040066 1102 52-53 0.30842958977997625 1102 54-55 0.27221244139332157 1102 56-57 0.3586288903654733 1102 58-59 0.37694362186972086 1102 60-61 0.39731938294349334 1102 62-63 0.3317102195954007 1102 64-65 0.2860014020127153 1102 66-67 0.25309991772677876 1102 68-69 0.2772729166636694 1102 70-71 0.27425055679047716 1102 72-73 0.29614879232217817 1102 74-75 0.27215074650877114 1102 76-77 0.23534098405054493 1102 78-79 0.23659927016708338 1102 80-81 0.2500895671448262 1102 82-83 0.22604554588365033 1102 84-85 0.2005485960713287 1102 86-87 0.19936126175164048 1102 88-89 0.23447118413631074 1102 90-91 0.2258747976586264 1102 92-93 0.26431513699192877 1102 94-95 0.2416691028687552 1102 96-97 0.26983688846364373 1102 98-99 0.28239797864866745 1102 100 0.3218916328349337 1103 1 0.16382898154491699 1103 2 0.1820626101670335 1103 3 0.3125758005207544 1103 4 0.2030213173239659 1103 5 0.17964478232736525 1103 6 0.18794013195650905 1103 7 0.15629914163513092 1103 8 0.1435009575324102 1103 9 0.2386968386533539 1103 10-11 0.2188474557516642 1103 12-13 0.2103583068567545 1103 14-15 0.3551353473531549 1103 16-17 0.30316703161857816 1103 18-19 0.304035021807195 1103 20-21 0.2561027873380368 1103 22-23 0.2513399948741579 1103 24-25 0.26542882556413616 1103 26-27 0.2650145999355118 1103 28-29 0.27862596150772845 1103 30-31 0.2886614541841013 1103 32-33 0.2720965540689235 1103 34-35 0.2777997881329597 1103 36-37 0.31283371863326437 1103 38-39 0.2777568398284984 1103 40-41 0.2074946052544533 1103 42-43 0.25954512225025184 1103 44-45 0.29322056782148564 1103 46-47 0.27134309528221934 1103 48-49 0.19696305289573246 1103 50-51 0.23799486051267849 1103 52-53 0.24109031559780192 1103 54-55 0.23552546053716128 1103 56-57 0.2976314290205764 1103 58-59 0.2580489389418048 1103 60-61 0.2092936326669914 1103 62-63 0.27483699646840165 1103 64-65 0.2680586034052652 1103 66-67 0.25933625018667783 1103 68-69 0.26309801668816846 1103 70-71 0.2394856793065685 1103 72-73 0.25194260453592676 1103 74-75 0.2508622305827899 1103 76-77 0.22865452280214527 1103 78-79 0.15053496499401575 1103 80-81 0.15442959104678522 1103 82-83 0.16521320846521803 1103 84-85 0.2125606658175343 1103 86-87 0.19267327879477847 1103 88-89 0.21599155647238888 1103 90-91 0.19372261938863033 1103 92-93 0.2099110899896992 1103 94-95 0.2041628119032488 1103 96-97 0.27218043085250443 1103 98-99 0.2427405857435403 1103 100 0.2271582287359699 1104 1 0.10871437894513036 1104 2 0.07547348125631359 1104 3 0.1914689925261328 1104 4 0.17861405821062704 1104 5 0.15208888604504267 1104 6 0.15135037619179315 1104 7 0.15162870471790058 1104 8 0.13213920729842243 1104 9 0.15011128785403827 1104 10-11 0.15794186068349347 1104 12-13 0.14838921436120245 1104 14-15 0.2673612194187456 1104 16-17 0.2705944156271727 1104 18-19 0.23511433468875964 1104 20-21 0.2332256355896547 1104 22-23 0.2521247728417535 1104 24-25 0.2330685545614628 1104 26-27 0.26556857314507454 1104 28-29 0.27805365822279526 1104 30-31 0.26825455389063535 1104 32-33 0.21185682204539802 1104 34-35 0.27116754319587955 1104 36-37 0.21101213136689267 1104 38-39 0.23968535973975946 1104 40-41 0.15508698155706213 1104 42-43 0.2048118414189446 1104 44-45 0.24083947373791403 1104 46-47 0.1896881575100835 1104 48-49 0.21555010238044758 1104 50-51 0.19152231203416648 1104 52-53 0.17253228873242676 1104 54-55 0.16912144088996683 1104 56-57 0.16079300676163655 1104 58-59 0.1915712099113378 1104 60-61 0.17977857988516632 1104 62-63 0.2135150118342466 1104 64-65 0.12214342179413507 1104 66-67 0.20280355975095432 1104 68-69 0.21539630119773534 1104 70-71 0.2615608686416877 1104 72-73 0.21655950668113633 1104 74-75 0.1996878520508858 1104 76-77 0.19728258438946256 1104 78-79 0.1842714903351279 1104 80-81 0.140349585743742 1104 82-83 0.172297811418062 1104 84-85 0.15009490321362762 1104 86-87 0.15125295734116406 1104 88-89 0.13719966983640575 1104 90-91 0.12956517960443392 1104 92-93 0.19483593596014614 1104 94-95 0.15282421924090173 1104 96-97 0.13683270341438458 1104 98-99 0.23735061411048264 1104 100 0.20038925757629755 1105 1 0.19530966265716643 1105 2 0.14707612303478967 1105 3 0.3080228047701823 1105 4 0.16380430261467893 1105 5 0.12878818863195107 1105 6 0.14104384388022595 1105 7 0.12107351125587229 1105 8 0.09730779874249862 1105 9 0.1875208209406125 1105 10-11 0.16497693504166477 1105 12-13 0.15565464307318422 1105 14-15 0.21025000762001866 1105 16-17 0.19874482839283303 1105 18-19 0.18149676189642605 1105 20-21 0.24941973178395926 1105 22-23 0.24189040309196486 1105 24-25 0.21723190405558057 1105 26-27 0.19000482146867625 1105 28-29 0.2544501225506721 1105 30-31 0.1974272789291902 1105 32-33 0.2186749756031432 1105 34-35 0.20179248215809054 1105 36-37 0.18230366378535479 1105 38-39 0.17708917850149675 1105 40-41 0.24074245443421205 1105 42-43 0.24466705663453325 1105 44-45 0.18429690711747782 1105 46-47 0.21009644252663406 1105 48-49 0.19827398690302545 1105 50-51 0.14757556339418443 1105 52-53 0.17570435375948534 1105 54-55 0.20876477900014123 1105 56-57 0.17755720116548446 1105 58-59 0.19935275811892694 1105 60-61 0.1134810110998501 1105 62-63 0.1468225119484643 1105 64-65 0.13543551418847954 1105 66-67 0.16650375134150863 1105 68-69 0.17459554685097345 1105 70-71 0.13233743420951782 1105 72-73 0.17275843839579608 1105 74-75 0.1751326507648443 1105 76-77 0.16416952500561877 1105 78-79 0.13092812130612685 1105 80-81 0.15223620571501328 1105 82-83 0.11318946404691133 1105 84-85 0.12221793276702897 1105 86-87 0.14666061014941079 1105 88-89 0.22362870721298833 1105 90-91 0.17265957172811142 1105 92-93 0.17670731028041686 1105 94-95 0.16739411290835093 1105 96-97 0.15629800607590383 1105 98-99 0.24196521084331835 1105 100 0.2590280662806812 1106 1 0.11744536922504523 1106 2 0.10742051583254053 1106 3 0.22483380016424803 1106 4 0.15600000474712772 1106 5 0.10711284369818941 1106 6 0.09541284124739491 1106 7 0.10118946373037119 1106 8 0.10361418114623433 1106 9 0.09339593856710593 1106 10-11 0.13408186104818043 1106 12-13 0.12096808091931877 1106 14-15 0.19054088892549714 1106 16-17 0.19625468147619785 1106 18-19 0.17505133769418535 1106 20-21 0.20255710992126552 1106 22-23 0.1630550668272619 1106 24-25 0.2019152722362847 1106 26-27 0.1613442197377566 1106 28-29 0.18439077464341125 1106 30-31 0.23352694752635728 1106 32-33 0.16687400764860882 1106 34-35 0.199753834196585 1106 36-37 0.1943452630189597 1106 38-39 0.2225741241845185 1106 40-41 0.24488684083719647 1106 42-43 0.22329692191294725 1106 44-45 0.16820871773484924 1106 46-47 0.2188337282466577 1106 48-49 0.21852310862858104 1106 50-51 0.2322479077657107 1106 52-53 0.23675986746728483 1106 54-55 0.21122662670100567 1106 56-57 0.27656505833945744 1106 58-59 0.2648863176414622 1106 60-61 0.17460354029573466 1106 62-63 0.19595886092422177 1106 64-65 0.22832532088731483 1106 66-67 0.2354471857018865 1106 68-69 0.22568598701320042 1106 70-71 0.24947177422046707 1106 72-73 0.29931742623968205 1106 74-75 0.23097890673632548 1106 76-77 0.24325465928645684 1106 78-79 0.2501994850154077 1106 80-81 0.21208826987727036 1106 82-83 0.210538023190054 1106 84-85 0.15400006548345857 1106 86-87 0.2050602107561943 1106 88-89 0.18059074719990065 1106 90-91 0.17756687117370262 1106 92-93 0.23079375180640582 1106 94-95 0.23166729721642199 1106 96-97 0.2546496295925742 1106 98-99 0.28959917892853326 1106 100 0.2988968004603727 1107 1 0.058732850172589224 1107 2 0.04497451210524517 1107 3 0.12253087284651798 1107 4 0.0914441741700287 1107 5 0.092592782223484 1107 6 0.06735079167482638 1107 7 0.06458089615170337 1107 8 0.0801479160414047 1107 9 0.02701321950844715 1107 10-11 0.09882841126547248 1107 12-13 0.052347243741593275 1107 14-15 0.07532561908335111 1107 16-17 0.1089640000826364 1107 18-19 0.1248378334658824 1107 20-21 0.11017025675865 1107 22-23 0.07790445613161268 1107 24-25 0.09953403769374347 1107 26-27 0.16189632501969697 1107 28-29 0.20383497474364987 1107 30-31 0.14318529874646657 1107 32-33 0.11553321816746376 1107 34-35 0.14752424904414596 1107 36-37 0.16255454377890288 1107 38-39 0.1086431875288767 1107 40-41 0.12973250551328164 1107 42-43 0.19656829592731384 1107 44-45 0.20017734549443134 1107 46-47 0.19640234960401415 1107 48-49 0.16784247532840624 1107 50-51 0.17333190463529036 1107 52-53 0.1996417227256302 1107 54-55 0.25738524117930695 1107 56-57 0.21376539832432684 1107 58-59 0.15111380273044261 1107 60-61 0.19039024880805755 1107 62-63 0.20100775625683553 1107 64-65 0.15760802817521835 1107 66-67 0.1789212535194693 1107 68-69 0.09101255888468529 1107 70-71 0.09928086451419915 1107 72-73 0.15523262609082877 1107 74-75 0.18265267198204782 1107 76-77 0.15978048476511475 1107 78-79 0.15784597011005275 1107 80-81 0.1783179557313801 1107 82-83 0.1658148410970739 1107 84-85 0.20521711194328418 1107 86-87 0.14994541584582777 1107 88-89 0.12316474876109851 1107 90-91 0.10227324742384525 1107 92-93 0.0974568616809357 1107 94-95 0.1162922451566537 1107 96-97 0.1229450669729708 1107 98-99 0.10180030752091085 1107 100 0.11574279058293868 1108 1 -0.04041044216462808 1108 2 -0.02514492193747131 1108 3 0.14305817039848634 1108 4 0.021442881091445543 1108 5 0.0647775876881056 1108 6 0.03285434631469286 1108 7 0.004355224098979704 1108 8 0.01913386392436678 1108 9 0.034841809011702196 1108 10-11 0.03430993052008091 1108 12-13 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-0.29766635118086526 2215 3 -0.19257440196182785 2215 4 -0.20693044962829532 2215 5 -0.21082929801684003 2215 6 -0.19293204169026268 2215 7 -0.21782234271269374 2215 8 -0.2023464559630881 2215 9 -0.3387357771329462 2215 10-11 -0.33367228703141905 2215 12-13 -0.3519657276858865 2215 14-15 -0.44715426287518767 2215 16-17 -0.4321895802227331 2215 18-19 -0.40478641189559994 2215 20-21 -0.4310463398316813 2215 22-23 -0.3981042946229465 2215 24-25 -0.41992259174892155 2215 26-27 -0.3690311119131451 2215 28-29 -0.3568242298576507 2215 30-31 -0.3280114927257429 2215 32-33 -0.32002151320249794 2215 34-35 -0.3760631595375372 2215 36-37 -0.3798881881681879 2215 38-39 -0.3397630492335395 2215 40-41 -0.38564322137833074 2215 42-43 -0.3416963700031346 2215 44-45 -0.33862216344716245 2215 46-47 -0.3732742942300362 2215 48-49 -0.3073441472145291 2215 50-51 -0.27263919049772056 2215 52-53 -0.2768004653822942 2215 54-55 -0.2546833022874466 2215 56-57 -0.2734524265286282 2215 58-59 -0.27262753946709495 2215 60-61 -0.32392307408011334 2215 62-63 -0.3191806883323096 2215 64-65 -0.3282555569827821 2215 66-67 -0.23394355805135802 2215 68-69 -0.2806976426626093 2215 70-71 -0.2792706180024851 2215 72-73 -0.25776082625819186 2215 74-75 -0.2343739644220122 2215 76-77 -0.22106765770348602 2215 78-79 -0.2181797370812646 2215 80-81 -0.27519404295156846 2215 82-83 -0.3182118255202653 2215 84-85 -0.2854786630676145 2215 86-87 -0.24255179250808823 2215 88-89 -0.22782183838912218 2215 90-91 -0.2641585201829528 2215 92-93 -0.20432276260142146 2215 94-95 -0.2215195729248265 2215 96-97 -0.20165383314005325 2215 98-99 -0.20381760044941544 2215 100 -0.21386814243420105 2216 1 -0.22349842060305747 2216 2 -0.1638398231250342 2216 3 -0.22963656785943698 2216 4 -0.16328682415078788 2216 5 -0.2492759214070972 2216 6 -0.257411984295679 2216 7 -0.32974789400373794 2216 8 -0.20345442771848354 2216 9 -0.3862432933969586 2216 10-11 -0.35556578039886233 2216 12-13 -0.3580254652065591 2216 14-15 -0.606700642166409 2216 16-17 -0.5366392581375479 2216 18-19 -0.48084195291717435 2216 20-21 -0.4586436832963443 2216 22-23 -0.5142378106740608 2216 24-25 -0.5196469244901394 2216 26-27 -0.5355845190129571 2216 28-29 -0.6017055802738653 2216 30-31 -0.5841054011429136 2216 32-33 -0.5528062213443192 2216 34-35 -0.6015441530183026 2216 36-37 -0.6337699556374403 2216 38-39 -0.5706533449006557 2216 40-41 -0.5802988954849724 2216 42-43 -0.6447151098908463 2216 44-45 -0.7157238532759607 2216 46-47 -0.7097782288276306 2216 48-49 -0.7446451314499569 2216 50-51 -0.6998347305923005 2216 52-53 -0.7287241935299278 2216 54-55 -0.6666381124448435 2216 56-57 -0.6480249309338646 2216 58-59 -0.6406617167166573 2216 60-61 -0.687239934432867 2216 62-63 -0.6419737115661732 2216 64-65 -0.674045900777827 2216 66-67 -0.6335094850662202 2216 68-69 -0.6551994112832844 2216 70-71 -0.658259231112055 2216 72-73 -0.6741312929859191 2216 74-75 -0.6699483068794763 2216 76-77 -0.5500545573411202 2216 78-79 -0.6035451147973632 2216 80-81 -0.6679668131327645 2216 82-83 -0.639891428204379 2216 84-85 -0.7149847238311295 2216 86-87 -0.6138799920105669 2216 88-89 -0.7154879011206994 2216 90-91 -0.6634838945203398 2216 92-93 -0.7071608540886274 2216 94-95 -0.6506185739706929 2216 96-97 -0.6822466594150747 2216 98-99 -0.7384420337197355 2216 100 -0.711646808094951 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 327.0 3 26.0 4 58.0 5 176.0 6 432.0 7 984.0 8 2174.0 9 3771.0 10 5077.0 11 6904.0 12 9663.0 13 12900.0 14 12783.0 15 14969.0 16 19188.0 17 27378.0 18 26553.0 19 16716.0 20 13943.0 21 14222.0 22 15421.0 23 16986.0 24 20457.0 25 25443.0 26 32809.0 27 42468.0 28 55718.0 29 72444.0 30 94361.0 31 122216.0 32 157594.0 33 205005.0 34 285961.0 35 424540.0 36 706883.0 37 1271483.0 38 1446015.0 39 425734.0 40 1001.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51096643638268 18.783634614808292 12.965336813518494 23.740062135290533 2 17.210129138838553 20.24701365210524 37.06448814719799 25.478369061858213 3 18.506454446732302 25.36984588425537 29.65867687272882 26.465022796283517 4 13.639094579134497 17.227666798020884 36.140089538305084 32.99314908453954 5 13.526649008794475 35.702524267691444 33.688653450593016 17.082173272921068 6 30.879976473838283 35.975760924292985 18.21646742205845 14.927795179810277 7 27.6012634956654 31.2586318166288 22.084404262292804 19.055700425412994 8 26.45508341267468 33.319443399827975 20.570177611732277 19.655295575765074 9 25.51148386954192 16.520849229064822 21.296867121754666 36.6707997796386 10-11 24.816233313603465 25.66581170578153 27.761660003603776 21.75629497701123 12-13 25.615726052196408 23.305762938094684 28.08133079429726 22.99718021541165 14-15 23.048157721316056 25.004604972657557 25.96547807725571 25.981759228770674 16-17 22.388372531962116 27.860024171314414 26.24513370059045 23.506469596133016 18-19 22.228291885159678 26.510146001038713 28.82866416838152 22.432897945420084 20-21 23.373802740313447 25.911925103481575 28.519476297553204 22.194795858651776 22-23 22.94916770083605 25.97294352677692 28.54793172361148 22.529957048775547 24-25 22.68479403177508 25.9891204084708 28.659123271708815 22.66696228804531 26-27 22.68320779969917 25.79249001097084 28.697299778524577 22.827002410805413 28-29 22.68790448950377 25.806064972117937 28.78825495795947 22.717775580418824 30-31 22.89643200391389 26.291742729289542 28.39054089157796 22.421284375218608 32-33 22.521241043908987 26.012174963661444 28.74267937780092 22.723904614628655 34-35 22.569902020787833 25.590309871244326 28.805040010465564 23.034748097502273 36-37 23.129988498927332 25.972409188667317 28.38025579683114 22.517346515574214 38-39 22.9526361415961 26.0992953559365 28.349304980273672 22.59876352219372 40-41 22.746504785387625 25.89505181856671 28.544915825851973 22.81352757019369 42-43 22.575824654495083 26.074486336466236 28.461438653561878 22.888250355476803 44-45 22.817730948850755 26.19339531761822 28.19300250214899 22.795871231382034 46-47 22.924029909920733 26.38286241994318 27.76960353376861 22.92350413636748 48-49 22.914021268577343 26.17904811740929 27.938032698033805 22.968897915979557 50-51 22.894269648763192 26.123450369494545 27.78487919578996 23.19740078595231 52-53 22.858250930063882 26.263549721023168 27.709037466422835 23.169161882490112 54-55 22.973534031817195 26.72021129358057 27.555167769708678 22.751086904893562 56-57 22.600129287044506 26.432205128314806 27.568073977711798 23.399591606928887 58-59 22.630665245473512 25.99087631065814 27.897349717156004 23.481108726712343 60-61 23.002353684284067 26.40101782676128 27.592100418159475 23.004528070795175 62-63 22.73786883447544 26.525649664025348 27.631015767812787 23.10546573368643 64-65 22.744273295578594 26.064028587851055 27.886165458425978 23.305532658144372 66-67 22.617496602063394 26.252379803228017 27.716255094231474 23.41386850047712 68-69 23.105784553338918 26.36728082665553 27.487157249659717 23.039777370345835 70-71 23.05520798513788 26.061192810581286 27.675364189982005 23.208235014298833 72-73 22.715945877954045 26.309779408809515 27.80809599263986 23.16617872059658 74-75 22.714846117103566 26.214666105839495 27.70621092329738 23.36427685375956 76-77 22.844593776308223 26.34283942692709 27.362476751183596 23.450090045581092 78-79 22.879888099883118 26.53365398154033 27.532483364874157 23.053974553702396 80-81 22.705874185811766 26.243063175508556 27.657710636938017 23.39335200174166 82-83 22.669718315130737 26.306753229254333 27.65565777621411 23.36787067940082 84-85 22.76610340592469 26.381751514020564 27.398643632915043 23.4535014471397 86-87 22.92092709240447 26.43261698177034 27.35167480402784 23.29478112179735 88-89 22.627898543976436 26.296273753496983 27.52086561339249 23.55496208913409 90-91 22.409095036749815 26.269988897124136 27.51247012743578 23.808445938690273 92-93 22.682321086226533 26.500801634155575 27.303424966445967 23.51345231317192 94-95 22.665569240508855 26.20854709141669 27.445432872594957 23.680450795479498 96-97 22.44286500283476 26.287329769549377 27.53829600319247 23.73150922442339 98-99 22.428461205443813 26.343010502973158 27.507741016846925 23.720787274736097 100 22.58663538389479 26.234806860251986 27.373441297393793 23.80511645845943 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 978.0 1 935.0 2 6375.0 3 9546.5 4 4468.0 5 1642.5 6 2326.5 7 4816.0 8 8075.0 9 9294.0 10 6707.5 11 3771.5 12 3172.0 13 3361.5 14 3756.5 15 4194.5 16 4577.0 17 5123.0 18 5754.0 19 6320.5 20 7028.0 21 8264.5 22 10075.5 23 12570.0 24 15883.0 25 20156.0 26 26158.5 27 33705.5 28 42919.0 29 53587.5 30 63329.5 31 72999.0 32 82603.5 33 91762.0 34 101377.0 35 111421.5 36 122487.0 37 134297.5 38 144954.5 39 153177.0 40 161332.5 41 168622.0 42 174486.0 43 179512.0 44 181691.5 45 184308.0 46 187517.0 47 187542.5 48 186244.5 49 189569.0 50 194635.0 51 194661.5 52 189670.0 53 186742.5 54 185995.5 55 180035.0 56 170094.5 57 157597.5 58 144974.5 59 135566.0 60 124226.0 61 108076.5 62 92490.0 63 76848.5 64 61726.5 65 49670.5 66 38671.0 67 29288.0 68 22329.5 69 16806.0 70 12581.0 71 9577.5 72 7198.5 73 5300.0 74 4079.0 75 2862.5 76 1765.0 77 1177.0 78 803.5 79 504.0 80 267.5 81 115.5 82 52.0 83 27.5 84 14.5 85 16.0 86 16.5 87 9.5 88 8.5 89 7.5 90 2.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08824080346718095 2 0.0 3 0.0 4 0.0 5 0.00789551119692207 6 1.4258259497827665E-4 7 0.0 8 8.91141218614229E-5 9 0.0 10-11 0.0 12-13 0.013901803010381972 14-15 8.91141218614229E-6 16-17 0.0 18-19 1.782282437228458E-5 20-21 2.673423655842687E-5 22-23 0.0 24-25 4.455706093071145E-5 26-27 4.455706093071145E-5 28-29 1.2475977060599208E-4 30-31 8.91141218614229E-6 32-33 1.1584835841984977E-4 34-35 1.782282437228458E-5 36-37 3.564564874456916E-5 38-39 1.2475977060599208E-4 40-41 8.020270967528062E-5 42-43 1.6040541935056124E-4 44-45 1.0693694623370748E-4 46-47 4.455706093071145E-5 48-49 3.1189942651498016E-4 50-51 1.782282437228458E-4 52-53 2.2278530465355727E-4 54-55 2.049624802812727E-4 56-57 2.1387389246741497E-4 58-59 8.020270967528062E-5 60-61 8.91141218614229E-5 62-63 3.1189942651498016E-4 64-65 1.4258259497827665E-4 66-67 4.4557060930711453E-4 68-69 5.346847311685375E-4 70-71 1.693168315367035E-4 72-73 3.4754507525954935E-4 74-75 4.099249605625454E-4 76-77 2.4060812902584186E-4 78-79 0.0011584835841984978 80-81 0.0010069895770340787 82-83 8.020270967528061E-4 84-85 0.001069369462337075 86-87 6.594445017745295E-4 88-89 0.0010069895770340787 90-91 0.0013278004157352013 92-93 0.0010069895770340787 94-95 0.001408003125410482 96-97 0.0014258259497827665 98-99 0.001532762896016474 100 4.812162580516837E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5610783.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.804584685460746 #Duplication Level Percentage of deduplicated Percentage of total 1 75.52620010956149 42.9023443009464 2 14.356603414108458 16.310417888645972 3 4.357296109951838 7.425431876321635 4 1.8646637138742095 4.236857913786932 5 1.0280182365884154 2.919807448924234 6 0.6293652376988716 2.1450498565770415 7 0.422319169849602 1.6792765533610639 8 0.3053450687093624 1.3875999851031047 9 0.2209704117212987 1.1296919219043275 >10 1.1792112908529708 11.95726761603297 >50 0.07568459595899836 2.937712474954145 >100 0.03265193379963787 3.175211288812058 >500 0.0012609148239155314 0.4767935696608258 >1k 2.521800006194174E-4 0.2493083741405519 >5k 6.304500013221019E-5 0.2648509775991661 >10k+ 9.45675001983153E-5 0.8023779532295742 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15827 0.2820818413401481 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14678 0.2616034161363931 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14305 0.25495550264553096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8069 0.1438123698599643 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6722 0.11980502543049697 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.782282437228458E-5 0.0 3 0.0 0.0 0.0 1.782282437228458E-5 0.0 4 0.0 0.0 0.0 1.782282437228458E-5 0.0 5 0.0 0.0 0.0 1.782282437228458E-5 0.0 6 0.0 0.0 0.0 1.782282437228458E-5 0.0 7 0.0 0.0 0.0 1.782282437228458E-5 0.0 8 0.0 1.782282437228458E-5 0.0 1.782282437228458E-5 0.0 9 0.0 1.782282437228458E-5 0.0 3.564564874456916E-5 0.0 10-11 0.0 1.782282437228458E-5 0.0 3.564564874456916E-5 0.0 12-13 0.0 1.782282437228458E-5 0.0 5.346847311685374E-5 0.0 14-15 0.0 3.564564874456916E-5 0.0 7.129129748913832E-5 0.0 16-17 0.0 5.346847311685374E-5 0.0 1.2475977060599205E-4 0.0 18-19 0.0 8.91141218614229E-5 0.0 1.693168315367035E-4 0.0 20-21 0.0 1.0693694623370748E-4 0.0 2.2278530465355727E-4 0.0 22-23 0.0 1.0693694623370748E-4 0.0 4.6339343367939913E-4 0.0 24-25 0.0 1.0693694623370748E-4 1.782282437228458E-5 9.446096917310829E-4 0.0 26-27 0.0 1.0693694623370748E-4 3.564564874456916E-5 0.001621877017877897 0.0 28-29 0.0 1.1584835841984978E-4 3.564564874456916E-5 0.0029942344945438097 0.0 30-31 0.0 1.2475977060599208E-4 3.564564874456916E-5 0.006950901505190987 0.0 32-33 0.0 1.2475977060599208E-4 3.564564874456916E-5 0.01588013651570556 0.0 34-35 0.0 1.4258259497827665E-4 3.564564874456916E-5 0.028997735253707014 0.0 36-37 0.0 2.2278530465355727E-4 3.564564874456916E-5 0.05096436629254776 0.0 38-39 0.0 2.940766021426956E-4 3.564564874456916E-5 0.08242165130963004 0.0 40-41 0.0 3.029880143288379E-4 3.564564874456916E-5 0.12408250327984524 0.0 42-43 0.0 3.38633663073407E-4 3.564564874456916E-5 0.1694594141316818 0.0 44-45 0.0 3.742793118179762E-4 3.564564874456916E-5 0.21928311966440334 0.0 46-47 0.0 3.742793118179762E-4 3.564564874456916E-5 0.27919454379183795 0.0 48-49 0.0 3.742793118179762E-4 3.564564874456916E-5 0.3478035062129474 0.0 50-51 0.0 3.921021361902608E-4 4.455706093071145E-5 0.42227617785253857 0.0 52-53 0.0 4.0992496056254534E-4 5.346847311685374E-5 0.4971231288039477 0.0 54-55 0.0 4.0992496056254534E-4 5.346847311685374E-5 0.5828330912102642 0.0 56-57 0.0 4.0992496056254534E-4 5.346847311685374E-5 0.6814824241108594 0.0 58-59 0.0 4.0992496056254534E-4 7.129129748913832E-5 0.7893550686241118 0.0 60-61 1.782282437228458E-5 4.0992496056254534E-4 7.129129748913832E-5 0.9028953712877508 0.0 62-63 1.782282437228458E-5 4.0992496056254534E-4 7.129129748913832E-5 1.0194566426824918 0.0 64-65 1.782282437228458E-5 4.0992496056254534E-4 7.129129748913832E-5 1.1506326300625065 0.0 66-67 1.782282437228458E-5 4.0992496056254534E-4 7.129129748913832E-5 1.29966708746355 0.0 68-69 1.782282437228458E-5 4.0992496056254534E-4 7.129129748913832E-5 1.4586466808643286 0.0 70-71 1.782282437228458E-5 4.0992496056254534E-4 7.129129748913832E-5 1.6313231147952076 0.0 72-73 1.782282437228458E-5 4.0992496056254534E-4 7.129129748913832E-5 1.8257433944602741 0.0 74-75 1.782282437228458E-5 4.2774778493482994E-4 7.129129748913832E-5 2.0184259487490426 0.0 76-77 1.782282437228458E-5 4.544820214932568E-4 7.129129748913832E-5 2.2192535337759454 0.0 78-79 1.782282437228458E-5 4.6339343367939913E-4 7.129129748913832E-5 2.4430903850674675 0.0 80-81 1.782282437228458E-5 4.6339343367939913E-4 7.129129748913832E-5 2.684803529204391 0.0 82-83 1.782282437228458E-5 4.6339343367939913E-4 7.129129748913832E-5 2.941790833828362 0.0 84-85 1.782282437228458E-5 4.6339343367939913E-4 7.129129748913832E-5 3.1959621322015126 0.0 86-87 1.782282437228458E-5 4.812162580516837E-4 7.129129748913832E-5 3.466485871936234 0.0 88 1.782282437228458E-5 4.812162580516837E-4 7.129129748913832E-5 3.685100635686677 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 895 0.0 49.88987 2 GGTATCA 8980 0.0 47.205223 1 TAACGCA 1090 0.0 41.395824 4 ATAACGC 1110 0.0 40.226517 3 TACCTGG 5915 0.0 40.128086 2 GTACCTG 6310 0.0 38.24984 1 GTATCAA 15270 0.0 37.743195 1 ATCAACG 18120 0.0 31.386219 3 TCAACGC 18335 0.0 31.069447 4 ACCTGGG 7820 0.0 30.112219 3 TATCACG 1005 0.0 29.93131 2 CAACGCA 19220 0.0 29.81001 5 TATCAAC 19355 0.0 29.286398 2 GTATAAC 1965 0.0 28.971066 1 AACGCAG 20485 0.0 28.41068 6 ACGCAGA 23270 0.0 24.95005 7 CGCAGAG 23730 0.0 24.506021 8 GTGGTAT 4415 0.0 24.296616 1 GTACATG 18220 0.0 22.51696 1 GCAGAGT 26200 0.0 22.016289 9 >>END_MODULE