FastQCFastQC Report
Wed 25 May 2016
SRR2049480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10330419
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90400.037.4475331
GTATCAA145550.035.049081
TCAACGC153000.031.9473514
ATCAACG155550.031.3937173
CAACGCA165550.029.5553115
AACGCAG176650.027.7242416
TATCAAC180300.027.4490782
GTACATG339350.024.1079641
ACGCAGA212050.022.9850857
TACATGG351050.022.639652
TATAACG9650.022.4040032
CGCAGAG219350.022.089868
ACATGGG352600.021.567183
TACCTGG86400.021.3239362
GTACCTG96650.020.383151
CATGGGG294600.018.4902884
ATAACGC11950.018.4853273
GCAGAGT260550.018.0738539
TATCACG15750.017.3078272
TAACGCA12350.016.364194
Produced by FastQC (version 0.11.5)