FastQCFastQC Report
Wed 25 May 2016
SRR2049480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10330419
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212400.2056063747269109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195890.18962444795317596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118780.11498081539577437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146200.049.8789021
GTATCAA246900.038.0721131
ATCAACG299350.030.9941563
TCAACGC300200.030.8750864
CAACGCA311750.029.8668865
TATCAAC321200.028.9296422
AACGCAG327600.028.4854566
ACGCAGA383450.024.311997
CGCAGAG391850.023.7308358
GTACATG341400.022.6553541
GCAGAGT439900.021.0425479
TACATGG353750.021.0327662
TACCTGG86150.020.9501862
GTGGTAT69850.020.6104561
ACATGGG351150.020.4121673
GTACCTG95200.019.619421
GAGTACT256150.019.38731212-13
AGAGTAC404700.019.32396110-11
ATAACGC10500.019.2482453
CAGAGTA426550.019.03946110-11
Produced by FastQC (version 0.11.5)