##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049480_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10330419 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.646158398802605 31.0 31.0 34.0 30.0 34.0 2 31.83810259777459 31.0 31.0 34.0 30.0 34.0 3 31.971832797875866 33.0 31.0 34.0 30.0 34.0 4 35.73428889960804 37.0 35.0 37.0 33.0 37.0 5 35.73511180911442 37.0 35.0 37.0 33.0 37.0 6 35.8373729081076 37.0 35.0 37.0 35.0 37.0 7 35.81077563262439 37.0 35.0 37.0 35.0 37.0 8 35.83273805254172 37.0 35.0 37.0 35.0 37.0 9 37.55275879903807 39.0 37.0 39.0 35.0 39.0 10-11 37.51582931921735 39.0 37.0 39.0 35.0 39.0 12-13 37.47723848374398 39.0 37.0 39.0 35.0 39.0 14-15 38.78463017811765 40.0 38.0 41.0 35.0 41.0 16-17 38.78504366570223 40.0 38.0 41.0 35.0 41.0 18-19 38.832686796150284 40.0 38.0 41.0 35.0 41.0 20-21 38.84491611618077 40.0 38.0 41.0 35.0 41.0 22-23 38.818072964901035 40.0 38.0 41.0 35.0 41.0 24-25 38.79721267840152 40.0 38.0 41.0 35.0 41.0 26-27 38.70087525007456 40.0 38.0 41.0 34.5 41.0 28-29 38.62571871479753 40.0 38.0 41.0 34.0 41.0 30-31 38.544701526627335 40.0 38.0 41.0 34.0 41.0 32-33 38.447140527407456 40.0 38.0 41.0 34.0 41.0 34-35 38.35419729828965 40.0 38.0 41.0 34.0 41.0 36-37 38.25420120907003 40.0 38.0 41.0 34.0 41.0 38-39 38.174152616655725 40.0 38.0 41.0 34.0 41.0 40-41 38.093649686426076 40.0 38.0 41.0 33.0 41.0 42-43 37.96803145158003 40.0 37.0 41.0 33.0 41.0 44-45 37.81629781909137 40.0 37.0 41.0 33.0 41.0 46-47 37.62132441094597 40.0 37.0 41.0 33.0 41.0 48-49 37.42509722984131 40.0 37.0 41.0 32.5 41.0 50-51 37.268037917919884 40.0 36.0 41.0 32.0 41.0 52-53 37.12783866753129 40.0 36.0 41.0 32.0 41.0 54-55 36.96250965231904 39.0 35.5 41.0 31.5 41.0 56-57 36.784127584757215 39.0 35.0 41.0 31.0 41.0 58-59 36.57050623987275 39.0 35.0 41.0 31.0 41.0 60-61 36.352559223396455 38.5 35.0 40.5 31.0 41.0 62-63 36.10677988956692 38.0 35.0 40.0 31.0 41.0 64-65 35.8574864194763 37.5 35.0 40.0 30.5 41.0 66-67 35.56602796072454 37.0 35.0 40.0 30.0 41.0 68-69 35.25370800545457 36.5 34.5 39.5 30.0 41.0 70-71 34.94478205579077 36.0 34.0 39.0 30.0 41.0 72-73 34.61953610013302 35.5 34.0 39.0 29.5 40.5 74-75 34.302563090616175 35.0 34.0 37.5 29.0 40.0 76-77 33.992290728962686 35.0 34.0 37.0 29.0 39.0 78-79 33.70090695256407 35.0 34.0 37.0 29.0 39.0 80-81 33.4241398630588 35.0 34.0 36.0 29.0 39.0 82-83 33.176892292558506 35.0 34.0 36.0 29.0 37.0 84-85 32.93883917002786 35.0 33.0 35.5 29.0 37.0 86-87 32.733729290167226 35.0 33.0 35.0 29.0 37.0 88-89 32.54709581479706 35.0 33.0 35.0 29.0 36.0 90-91 32.384069126334566 35.0 33.0 35.0 28.0 36.0 92-93 32.237144398499225 35.0 33.0 35.0 27.0 36.0 94-95 32.094096570526325 35.0 33.0 35.0 27.0 35.5 96-97 31.941385436544248 35.0 33.0 35.0 27.0 35.0 98-99 31.80201504895397 35.0 33.0 35.0 27.0 35.0 100 31.69141212955641 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15654031784323763 1101 2 0.21892296375463616 1101 3 0.3751332517984203 1101 4 0.22181182125216736 1101 5 0.21056799573293006 1101 6 0.18427619880907997 1101 7 0.1734582538419147 1101 8 0.18519970885201786 1101 9 0.2755338175422395 1101 10-11 0.2885977268279163 1101 12-13 0.23417505306593256 1101 14-15 0.35417268247982747 1101 16-17 0.3248225465571082 1101 18-19 0.3540424978072778 1101 20-21 0.3456387148917486 1101 22-23 0.3570766921676878 1101 24-25 0.335491121460727 1101 26-27 0.3480472868053326 1101 28-29 0.37408291597769505 1101 30-31 0.3529034305098193 1101 32-33 0.38010953669227376 1101 34-35 0.35737816176335 1101 36-37 0.40051914687840906 1101 38-39 0.3857981087515725 1101 40-41 0.4171617208925724 1101 42-43 0.39436637162623356 1101 44-45 0.37267205093276345 1101 46-47 0.3804899113034992 1101 48-49 0.40175104887670443 1101 50-51 0.3788700489597119 1101 52-53 0.3747412909835006 1101 54-55 0.40843549243026445 1101 56-57 0.43167706056041055 1101 58-59 0.41472650406949896 1101 60-61 0.36700813602978855 1101 62-63 0.36169917053117473 1101 64-65 0.3287651127155016 1101 66-67 0.3528780364944666 1101 68-69 0.36557284132187107 1101 70-71 0.34924066286414757 1101 72-73 0.3416720548648655 1101 74-75 0.30220198351924665 1101 76-77 0.29445444887002736 1101 78-79 0.3006343007102643 1101 80-81 0.2797231254831871 1101 82-83 0.26871306418022556 1101 84-85 0.28877297596983453 1101 86-87 0.27321579081628045 1101 88-89 0.32300486391829963 1101 90-91 0.31975173128450507 1101 92-93 0.32027948256477856 1101 94-95 0.3442550428120015 1101 96-97 0.3073491904916388 1101 98-99 0.33840228601416555 1101 100 0.3274023735802274 1102 1 0.14831314421167718 1102 2 0.16504118686010827 1102 3 0.29485506529043803 1102 4 0.20754421013921842 1102 5 0.14765703553953813 1102 6 0.1308629427168171 1102 7 0.16271975358579738 1102 8 0.16955570836374534 1102 9 0.186360859066923 1102 10-11 0.21300976110268977 1102 12-13 0.21150950592328144 1102 14-15 0.23558060410097426 1102 16-17 0.23720082398839537 1102 18-19 0.26350029833045596 1102 20-21 0.23687931938908946 1102 22-23 0.22401229633989317 1102 24-25 0.24536904238598112 1102 26-27 0.2536615141911511 1102 28-29 0.24661061335940815 1102 30-31 0.22865711621182783 1102 32-33 0.2538966917512937 1102 34-35 0.2667833271650366 1102 36-37 0.30978647164570816 1102 38-39 0.24650201252118364 1102 40-41 0.2595766391585741 1102 42-43 0.203794291809956 1102 44-45 0.29426847241073517 1102 46-47 0.23330672652384976 1102 48-49 0.20411359440965526 1102 50-51 0.22697955620562738 1102 52-53 0.20134105620288523 1102 54-55 0.1738180878626565 1102 56-57 0.2099779950055094 1102 58-59 0.23755320563002869 1102 60-61 0.22555665108232859 1102 62-63 0.24403876049789375 1102 64-65 0.2063662936916586 1102 66-67 0.22366878855678607 1102 68-69 0.24830123391886616 1102 70-71 0.22484473923972104 1102 72-73 0.2438141142490693 1102 74-75 0.19446978857148167 1102 76-77 0.18217210977973508 1102 78-79 0.19804247559330435 1102 80-81 0.22585559742188366 1102 82-83 0.2538322193350808 1102 84-85 0.25020424103577454 1102 86-87 0.18984192493327612 1102 88-89 0.18601266369994818 1102 90-91 0.21766433311689326 1102 92-93 0.20643375099728445 1102 94-95 0.13366156578867816 1102 96-97 0.17300217835445508 1102 98-99 0.21266258447531072 1102 100 0.27765006142468707 1103 1 0.13069198645654367 1103 2 0.12516213314808766 1103 3 0.2877226650421214 1103 4 0.17093289044857585 1103 5 0.16880197606442238 1103 6 0.1505465204060954 1103 7 0.16446569863011717 1103 8 0.14822108029790826 1103 9 0.18608880644192993 1103 10-11 0.2083901465178215 1103 12-13 0.19709953973218575 1103 14-15 0.2470899561716351 1103 16-17 0.27270318921619463 1103 18-19 0.24705660278324615 1103 20-21 0.2608257172722972 1103 22-23 0.2868701728513585 1103 24-25 0.28019359741005445 1103 26-27 0.2758865105858632 1103 28-29 0.29177991755581445 1103 30-31 0.28360530463495337 1103 32-33 0.2977705803891695 1103 34-35 0.30066791113700475 1103 36-37 0.3108642105826078 1103 38-39 0.2730905061516964 1103 40-41 0.2911691701387369 1103 42-43 0.3294344490876995 1103 44-45 0.3314535085063852 1103 46-47 0.30101602155769314 1103 48-49 0.32891202124628904 1103 50-51 0.3191269788538804 1103 52-53 0.30487494053458875 1103 54-55 0.27181283620117114 1103 56-57 0.2856486410844852 1103 58-59 0.3090026522163285 1103 60-61 0.2587058910026414 1103 62-63 0.27252606806057855 1103 64-65 0.27933066697329423 1103 66-67 0.2966938203370475 1103 68-69 0.26523438430995583 1103 70-71 0.26803505742901024 1103 72-73 0.28688476628347104 1103 74-75 0.25206951086881446 1103 76-77 0.25411669621530564 1103 78-79 0.2503065160474236 1103 80-81 0.26132133873401386 1103 82-83 0.2488340174697541 1103 84-85 0.28052492632602366 1103 86-87 0.21630927417471923 1103 88-89 0.27665889970677426 1103 90-91 0.25274241415436194 1103 92-93 0.25629602604323054 1103 94-95 0.2153066302904918 1103 96-97 0.27373643984300244 1103 98-99 0.34147060866325774 1103 100 0.32171002610922983 1104 1 0.10687703251621627 1104 2 0.15810337006437436 1104 3 0.24512027615000065 1104 4 0.16829428743785257 1104 5 0.11406178075088036 1104 6 0.12322538429014429 1104 7 0.14303536276906925 1104 8 0.14862806445373167 1104 9 0.16887970124889762 1104 10-11 0.17863819310802853 1104 12-13 0.15336164306596345 1104 14-15 0.2661036825094669 1104 16-17 0.23034520123994895 1104 18-19 0.18507752810538136 1104 20-21 0.21941573751954024 1104 22-23 0.19789413605937511 1104 24-25 0.22708617758276972 1104 26-27 0.1916126410115453 1104 28-29 0.22680714996099027 1104 30-31 0.2232584964256361 1104 32-33 0.25161525210417324 1104 34-35 0.24319313635114526 1104 36-37 0.22807900910684253 1104 38-39 0.24139920802549142 1104 40-41 0.2546695224215867 1104 42-43 0.25530262757304456 1104 44-45 0.26980054806105613 1104 46-47 0.26993032246620174 1104 48-49 0.25302695104000605 1104 50-51 0.252478333260143 1104 52-53 0.26892143048983996 1104 54-55 0.2594825848770981 1104 56-57 0.24360941107639889 1104 58-59 0.2802591523512561 1104 60-61 0.26811645930767725 1104 62-63 0.22521106963338156 1104 64-65 0.2057685086945682 1104 66-67 0.2791477560654343 1104 68-69 0.2833404852063097 1104 70-71 0.25082696388206216 1104 72-73 0.23748680986138737 1104 74-75 0.23926087021344244 1104 76-77 0.2573238656246204 1104 78-79 0.2353802083523746 1104 80-81 0.23236732064273724 1104 82-83 0.22957133887354075 1104 84-85 0.2714182756734971 1104 86-87 0.22100995693487846 1104 88-89 0.2015114302463914 1104 90-91 0.19812080931956189 1104 92-93 0.23432750937247704 1104 94-95 0.22921836557933517 1104 96-97 0.1840723836122784 1104 98-99 0.1942515168408221 1104 100 0.1912778785029161 1105 1 0.1035239947978539 1105 2 0.10237225717112253 1105 3 0.2601717908529402 1105 4 0.17982617637798626 1105 5 0.15499120530935073 1105 6 0.13270244392570874 1105 7 0.13982723606154934 1105 8 0.14147418127577538 1105 9 0.16989595144497116 1105 10-11 0.19959756382415605 1105 12-13 0.17850166942093182 1105 14-15 0.22771112276053884 1105 16-17 0.21605339766342269 1105 18-19 0.20332921494393474 1105 20-21 0.21799385366624335 1105 22-23 0.1700020072992956 1105 24-25 0.21503270307217548 1105 26-27 0.19917782259113892 1105 28-29 0.22586891686935928 1105 30-31 0.24510921034248412 1105 32-33 0.27510064705013804 1105 34-35 0.23661672910063203 1105 36-37 0.24427598628948033 1105 38-39 0.23492164293216433 1105 40-41 0.24676348867374287 1105 42-43 0.27039352524594307 1105 44-45 0.2552155702379082 1105 46-47 0.22233625597117168 1105 48-49 0.20974531459874157 1105 50-51 0.21015954039877727 1105 52-53 0.18276076291608945 1105 54-55 0.2458769724612253 1105 56-57 0.21873649000406914 1105 58-59 0.2079908929295513 1105 60-61 0.1603557107821274 1105 62-63 0.2013273643523661 1105 64-65 0.16422397045856485 1105 66-67 0.20697425836957706 1105 68-69 0.23938684215740125 1105 70-71 0.23123527618361095 1105 72-73 0.19942649995580553 1105 74-75 0.23316480894653324 1105 76-77 0.22362381841250567 1105 78-79 0.21227448124502502 1105 80-81 0.1988147238807798 1105 82-83 0.13608649715816057 1105 84-85 0.19041033470440993 1105 86-87 0.1974822166835679 1105 88-89 0.20462544298105456 1105 90-91 0.2263550589361003 1105 92-93 0.23061132151705976 1105 94-95 0.20001414916278293 1105 96-97 0.21105859472908506 1105 98-99 0.21915628095565154 1105 100 0.22994518041861411 1106 1 0.08653051450065163 1106 2 0.10558230028416915 1106 3 0.2294505987755464 1106 4 0.1717607644131789 1106 5 0.14801332840769987 1106 6 0.10769907935969769 1106 7 0.14742746941344365 1106 8 0.1349344817269298 1106 9 0.1848549602275824 1106 10-11 0.16574018668306678 1106 12-13 0.12796780847092037 1106 14-15 0.19499115957940916 1106 16-17 0.21421517955453595 1106 18-19 0.22074580442212266 1106 20-21 0.20896443175446677 1106 22-23 0.18982809206998041 1106 24-25 0.17359380422649195 1106 26-27 0.20124101824958274 1106 28-29 0.22059669488172062 1106 30-31 0.2188160272144799 1106 32-33 0.1949261669409026 1106 34-35 0.15116984341777595 1106 36-37 0.22180997758552223 1106 38-39 0.2206144490875488 1106 40-41 0.19884164658903813 1106 42-43 0.22045027449475896 1106 44-45 0.2020709331584314 1106 46-47 0.2202040037426798 1106 48-49 0.263215322511293 1106 50-51 0.24313409495918847 1106 52-53 0.21900220163134776 1106 54-55 0.20962071977010766 1106 56-57 0.23275082185492835 1106 58-59 0.2144808406795633 1106 60-61 0.1672667059014259 1106 62-63 0.18950414521750503 1106 64-65 0.17026462684337673 1106 66-67 0.16433309734927803 1106 68-69 0.19581098845791445 1106 70-71 0.21641522786095635 1106 72-73 0.2738375949882368 1106 74-75 0.24780547965106337 1106 76-77 0.21950427626461533 1106 78-79 0.21409645905625752 1106 80-81 0.2040839378828565 1106 82-83 0.18029884342784896 1106 84-85 0.16402557041961785 1106 86-87 0.16965981888411363 1106 88-89 0.1881353836672517 1106 90-91 0.17991270796802183 1106 92-93 0.2194368246026528 1106 94-95 0.2103784811760434 1106 96-97 0.18936107691030557 1106 98-99 0.20973330720462968 1106 100 0.19299223307996982 1107 1 0.03650193346832609 1107 2 0.06402125945340842 1107 3 0.16778686555911904 1107 4 0.11684250166075572 1107 5 0.11459270218120565 1107 6 0.12095062810441704 1107 7 0.09558704009657504 1107 8 0.11611425914111351 1107 9 0.1272500026976786 1107 10-11 0.12123833034615927 1107 12-13 0.0803136945952474 1107 14-15 0.1085645576378127 1107 16-17 0.11859774603575346 1107 18-19 0.12016291561118919 1107 20-21 0.11547431525823271 1107 22-23 0.11629415989539638 1107 24-25 0.12704903346507734 1107 26-27 0.15667685113606922 1107 28-29 0.16798336277147996 1107 30-31 0.13283601573821358 1107 32-33 0.11002420963900761 1107 34-35 0.09328235654794526 1107 36-37 0.09765740763575081 1107 38-39 0.09878781724236063 1107 40-41 0.1529302072653067 1107 42-43 0.16475698607700906 1107 44-45 0.1795387583430923 1107 46-47 0.13482869383577167 1107 48-49 0.15341856348096172 1107 50-51 0.16418385733008733 1107 52-53 0.14737723564464744 1107 54-55 0.20070020254760124 1107 56-57 0.13348239542109752 1107 58-59 0.1221301682264837 1107 60-61 0.1403871683996698 1107 62-63 0.14483416539555805 1107 64-65 0.1198913252929259 1107 66-67 0.13939764411801292 1107 68-69 0.12632604881913068 1107 70-71 0.14415577631180554 1107 72-73 0.16272261221050854 1107 74-75 0.17284432770938452 1107 76-77 0.16809513878190785 1107 78-79 0.165230320843186 1107 80-81 0.13315773562921152 1107 82-83 0.15533103683077343 1107 84-85 0.11616430610126827 1107 86-87 0.10703303733360059 1107 88-89 0.08722134078559662 1107 90-91 0.1161859478594991 1107 92-93 0.18462767667102753 1107 94-95 0.14032941173845614 1107 96-97 0.16861160424464927 1107 98-99 0.21270166024688564 1107 100 0.2101155733553952 1108 1 0.05689830681690111 1108 2 0.07319284444489327 1108 3 0.15686256888242056 1108 4 0.08471587846622697 1108 5 0.07755610743006258 1108 6 0.07908770136084087 1108 7 0.0552248488203233 1108 8 0.01892522816517328 1108 9 0.12177707116506298 1108 10-11 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92-93 -0.31918590466786156 2214 94-95 -0.2916236177608056 2214 96-97 -0.2942632587795728 2214 98-99 -0.33536095566513424 2214 100 -0.30311952166020006 2215 1 -0.2296191018673781 2215 2 -0.31433308474448296 2215 3 -0.24388895551992107 2215 4 -0.27325790257227567 2215 5 -0.2358343134148555 2215 6 -0.21246327438002766 2215 7 -0.24881501285938867 2215 8 -0.29548772467761353 2215 9 -0.4059819418454964 2215 10-11 -0.4243220895554458 2215 12-13 -0.4087463386225849 2215 14-15 -0.5076830464741064 2215 16-17 -0.5416312134603629 2215 18-19 -0.48901784632280965 2215 20-21 -0.48808075211701407 2215 22-23 -0.45022417064331677 2215 24-25 -0.4411400456005694 2215 26-27 -0.47288225630743597 2215 28-29 -0.3894528291192074 2215 30-31 -0.4366096793238441 2215 32-33 -0.38990654779711065 2215 34-35 -0.44303894316503545 2215 36-37 -0.4392555981336983 2215 38-39 -0.40528481020307083 2215 40-41 -0.46218738563840844 2215 42-43 -0.4460080304450571 2215 44-45 -0.43934093363685633 2215 46-47 -0.459683093464335 2215 48-49 -0.453214018536805 2215 50-51 -0.46772196552997514 2215 52-53 -0.46030391617984634 2215 54-55 -0.38887350035900425 2215 56-57 -0.3746224228837747 2215 58-59 -0.40461287963135106 2215 60-61 -0.4070127406626156 2215 62-63 -0.4338306496247313 2215 64-65 -0.4662271677044032 2215 66-67 -0.37481095966766986 2215 68-69 -0.372011669997228 2215 70-71 -0.3608597374934135 2215 72-73 -0.3348694972201258 2215 74-75 -0.34017430579880426 2215 76-77 -0.34084174846424276 2215 78-79 -0.38315791303212876 2215 80-81 -0.454848741477619 2215 82-83 -0.3646072891326426 2215 84-85 -0.32868178128597236 2215 86-87 -0.3030420269766765 2215 88-89 -0.3728136114085814 2215 90-91 -0.3642318956801134 2215 92-93 -0.3166445547785308 2215 94-95 -0.3605077694586072 2215 96-97 -0.34572925405050725 2215 98-99 -0.3374085623402969 2215 100 -0.29226327960113707 2216 1 -0.1480651067036689 2216 2 -0.20822741846077975 2216 3 -0.26076719396536063 2216 4 -0.21720301838681877 2216 5 -0.24095183768756812 2216 6 -0.25883660562654853 2216 7 -0.33316975594015474 2216 8 -0.24622445433044504 2216 9 -0.4554780418975355 2216 10-11 -0.36623034781730723 2216 12-13 -0.3616345725879384 2216 14-15 -0.5802864181817569 2216 16-17 -0.5433823703820124 2216 18-19 -0.5064920856514448 2216 20-21 -0.4759191287296005 2216 22-23 -0.46361207861701104 2216 24-25 -0.4867533581098087 2216 26-27 -0.5290395626274034 2216 28-29 -0.540944608012417 2216 30-31 -0.5752847135565133 2216 32-33 -0.5722075584510691 2216 34-35 -0.5541233814149678 2216 36-37 -0.6552058781270205 2216 38-39 -0.5899594471734204 2216 40-41 -0.6234392158249378 2216 42-43 -0.6665452829978946 2216 44-45 -0.7374044350033273 2216 46-47 -0.7066943419472054 2216 48-49 -0.6832130864710919 2216 50-51 -0.6860675119712383 2216 52-53 -0.6746775896497823 2216 54-55 -0.6535020499961846 2216 56-57 -0.6540084797273025 2216 58-59 -0.6712784239723177 2216 60-61 -0.6667257730858225 2216 62-63 -0.6547643828986267 2216 64-65 -0.6942003452174248 2216 66-67 -0.6599448393588077 2216 68-69 -0.6728039513020363 2216 70-71 -0.7326847756682682 2216 72-73 -0.7193712388590896 2216 74-75 -0.6992433390775759 2216 76-77 -0.6083816840001077 2216 78-79 -0.6590350744315643 2216 80-81 -0.6891478932482471 2216 82-83 -0.6544456567494379 2216 84-85 -0.7395469552323632 2216 86-87 -0.647085145024711 2216 88-89 -0.7595467767982385 2216 90-91 -0.739619918060967 2216 92-93 -0.7107341883686367 2216 94-95 -0.7368434332933234 2216 96-97 -0.7081186082611595 2216 98-99 -0.8115234817036558 2216 100 -0.8056551880909026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 608.0 3 70.0 4 90.0 5 207.0 6 465.0 7 1238.0 8 2588.0 9 4259.0 10 5847.0 11 8021.0 12 11198.0 13 14818.0 14 17368.0 15 22056.0 16 29718.0 17 41690.0 18 40815.0 19 25516.0 20 21734.0 21 23002.0 22 24957.0 23 27978.0 24 33359.0 25 42392.0 26 54941.0 27 72847.0 28 96712.0 29 128451.0 30 166995.0 31 214924.0 32 280182.0 33 368056.0 34 509581.0 35 764247.0 36 1306654.0 37 2349649.0 38 2776497.0 39 838856.0 40 1833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.89993558758693 19.481780433508717 14.442758990608654 24.1755249882957 2 15.855165216435074 19.692705591128494 38.374203408399985 26.07792578403645 3 18.199116608919734 24.327309473120113 29.843736251162706 27.629837666797446 4 13.50090577167352 16.69860793629067 36.07987595468064 33.720610337355176 5 14.087225080640172 36.738645101824105 34.334760084414306 14.839369733121424 6 32.52192628578403 36.13065078062253 16.94943539989559 14.397987533697842 7 28.500160545278945 31.567354625209298 21.24149078561092 18.690994043900833 8 26.40888917456116 35.18172757552267 19.354317854628782 19.05506539528739 9 25.751830588865754 16.29248532900747 20.203701321311364 37.75198276081541 10-11 25.284835978095373 26.075544467267008 27.34405061401672 21.2955689406209 12-13 25.685642751610654 23.265708933622857 27.785238745952572 23.263409568813913 14-15 22.853237884468953 25.00278304324181 25.27138785671596 26.872591215573273 16-17 22.63404320773436 28.26563956408738 25.922631986175972 23.177685242002287 18-19 22.64927337157477 27.079825186155354 28.058401318242947 22.21250012402693 20-21 23.40990945380913 26.163491157860214 28.022704405571975 22.403894982758686 22-23 23.23595490173245 25.98931853586965 27.99713157810927 22.777594984288633 24-25 23.06117681998479 26.154643723931347 27.89437521199534 22.889804244088523 26-27 22.905540845759624 26.242792220159544 27.921717587890328 22.929949346190508 28-29 22.879601525787226 26.226411258803502 28.112474777126355 22.781512438282913 30-31 23.031643056457156 26.350114777543364 27.8494055129231 22.76883665307638 32-33 22.897840987027145 26.251171903548887 27.99067607642094 22.86031103300303 34-35 23.04018578919072 26.124688274956544 27.971230205786448 22.863895730066293 36-37 23.180657817620425 26.164008004904737 27.79111084243312 22.864223335041718 38-39 23.019229305106105 26.269750618187317 27.87672544671411 22.834294629992467 40-41 23.018990716346757 26.22357124824402 27.88387224677109 22.873565788638135 42-43 23.03578980831234 26.287629354877772 27.75280509494357 22.92377574186631 44-45 23.028148842538343 26.385717426234994 27.633906389167436 22.952227342059224 46-47 23.17171300338433 26.33501298520016 27.471971236004133 23.021302775411385 48-49 23.19404316749141 26.39487601120945 27.24818362366685 23.162897197632287 50-51 23.128883985991248 26.51142516373278 27.203842755734797 23.15584809454117 52-53 23.168445002904292 26.562332487657418 27.146603438367872 23.122619071070417 54-55 23.186499996079533 26.666700224589622 27.097491993773325 23.049307785557517 56-57 23.018467147299926 26.539279847758056 27.203276465894493 23.238976539047524 58-59 23.069567647952102 26.292681923398508 27.32795036016229 23.3098000684871 60-61 23.133175588714504 26.43324773976794 27.245437148891614 23.18813952262594 62-63 22.9549300219353 26.506822595102985 27.337735881932705 23.200511501029002 64-65 22.98804427029828 26.35950535325221 27.35632517706837 23.296125199381144 66-67 22.90577603930006 26.362754233489888 27.32308322860971 23.40838649860034 68-69 23.03280257590186 26.474492061629007 27.215279231488733 23.2774261309804 70-71 23.041225601987765 26.31806354854957 27.236754894606708 23.403955954855963 72-73 22.99948230345149 26.47459782683612 27.16507124232458 23.360848627387814 74-75 22.986656831597678 26.510922153880106 27.082169290965098 23.42025172355712 76-77 23.017328575979636 26.471282360700123 27.018952715545286 23.49243634777496 78-79 23.007778089697297 26.521538580680136 27.09031683494187 23.380366494680697 80-81 22.92214225800472 26.5173230438762 27.11289539573575 23.447639302383326 82-83 22.889687776415823 26.49655928472811 27.157970395449325 23.455782543406738 84-85 22.949117135570667 26.440000019360532 26.974704013776957 23.636178831291847 86-87 22.964953361384378 26.546265273404263 26.975476931555313 23.513304433656046 88-89 22.864703272670738 26.42986768353202 27.062443146666766 23.642985897130472 90-91 22.80892396594572 26.40409534211222 27.04322659445818 23.743754097483876 92-93 22.85394796171812 26.54419738095187 26.966323785966708 23.635530871363304 94-95 22.837475971713232 26.379168603732378 27.035375881904372 23.747979542650015 96-97 22.828766343892056 26.4072001578661 26.99774599385002 23.766287504391826 98-99 22.702392330787685 26.423859775951954 27.06675387342181 23.806994019838555 100 22.82812274330489 26.27016723115102 26.990925399395106 23.91078462614899 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 845.0 1 737.5 2 2791.0 3 4190.0 4 2213.5 5 1031.0 6 2075.0 7 5436.0 8 9294.5 9 10651.0 10 8039.0 11 4929.5 12 4363.5 13 4776.0 14 5349.0 15 5978.0 16 6688.0 17 7440.5 18 8439.0 19 9491.5 20 10825.5 21 12867.5 22 16047.5 23 20627.5 24 26536.0 25 34592.0 26 45296.5 27 58270.0 28 74035.0 29 92641.5 30 111483.5 31 130133.5 32 149641.0 33 168672.0 34 187879.0 35 209104.5 36 231061.0 37 253813.0 38 276644.5 39 294052.0 40 309897.0 41 325226.0 42 336367.5 43 344985.0 44 351020.5 45 356736.0 46 359719.0 47 356102.5 48 350597.0 49 350938.5 50 353469.5 51 351511.0 52 343263.5 53 338206.0 54 334764.5 55 321404.0 56 303409.5 57 279748.0 58 256338.5 59 240359.0 60 219374.5 61 192230.0 62 168533.5 63 144190.0 64 119011.0 65 97345.0 66 76954.0 67 59941.0 68 46591.5 69 35681.0 70 26798.5 71 19678.5 72 15076.5 73 11247.5 74 8229.5 75 5861.5 76 3840.0 77 2672.5 78 1832.0 79 1150.0 80 661.5 81 319.5 82 154.5 83 95.5 84 65.5 85 57.0 86 47.0 87 45.5 88 41.0 89 29.0 90 17.5 91 10.5 92 6.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09125476904663789 2 0.0 3 0.0 4 9.680149469251924E-6 5 0.008615333027634214 6 9.680149469251926E-5 7 0.0 8 1.0648164416177116E-4 9 0.0 10-11 0.0 12-13 0.014321781139758221 14-15 9.680149469251924E-6 16-17 0.0 18-19 1.4520224203877887E-5 20-21 9.680149469251924E-6 22-23 0.0 24-25 2.9040448407755774E-5 26-27 1.4520224203877887E-5 28-29 1.3552209256952695E-4 30-31 9.680149469251924E-6 32-33 3.87205978770077E-5 34-35 9.680149469251924E-6 36-37 2.4200373673129815E-5 38-39 1.4036216730415292E-4 40-41 2.9040448407755774E-5 42-43 1.9360298938503852E-4 44-45 1.161617936310231E-4 46-47 4.356067261163366E-5 48-49 2.8556440934293176E-4 50-51 1.9360298938503852E-4 52-53 2.2748351252742025E-4 54-55 2.1296328832354233E-4 56-57 1.9360298938503852E-4 58-59 6.292097155013751E-5 60-61 8.228127048864137E-5 62-63 3.775258293008251E-4 64-65 1.5972246624265676E-4 66-67 5.130479218703521E-4 68-69 5.469284450127337E-4 70-71 1.7424269044653465E-4 72-73 4.1624642717783277E-4 74-75 3.775258293008251E-4 76-77 2.5168388620055006E-4 78-79 0.001151937786840979 80-81 0.0010067355448022002 82-83 7.889321817440318E-4 84-85 0.0012003385341872387 86-87 8.179726301517877E-4 88-89 0.0010648164416177117 90-91 0.001418141897245407 92-93 9.776950963944445E-4 94-95 0.001418141897245407 96-97 0.001408461747776155 98-99 0.0017375868297307205 100 4.549670250548405E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0330419E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.50515530125218 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99416028089719 36.36076245769954 2 15.068502386982555 15.220741064236861 3 5.103479826341779 7.732561236185973 4 2.3808056527861265 4.809718369442496 5 1.3708636426603977 3.461784058470183 6 0.8475499590698236 2.5683385385034825 7 0.5956260626534412 2.1057530757049787 8 0.4490937887556389 1.8145241236745027 9 0.321600569008861 1.4618238014487235 >10 1.7144065702611595 15.53112229533291 >50 0.10629811620307733 3.654776634581367 >100 0.045729671522677026 3.931970581866462 >500 0.001499686567508628 0.49826779452325437 >1k 2.8784021837513214E-4 0.22585277279406737 >5k 3.837842853563626E-5 0.11119106672448287 >10k+ 5.756764280345439E-5 0.5108121288108881 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21240 0.2056063747269109 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19589 0.18962444795317596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11878 0.11498081539577437 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.680149469251924E-6 0.0 9.680149469251924E-6 0.0 6 0.0 1.936029893850385E-5 0.0 9.680149469251924E-6 0.0 7 0.0 1.936029893850385E-5 0.0 9.680149469251924E-6 0.0 8 0.0 1.936029893850385E-5 0.0 9.680149469251924E-6 0.0 9 0.0 1.936029893850385E-5 0.0 9.680149469251924E-6 0.0 10-11 0.0 1.936029893850385E-5 0.0 9.680149469251924E-6 0.0 12-13 0.0 1.936029893850385E-5 0.0 9.680149469251924E-6 0.0 14-15 0.0 4.840074734625962E-5 0.0 9.680149469251924E-6 0.0 16-17 9.680149469251924E-6 5.808089681551155E-5 0.0 9.680149469251924E-6 0.0 18-19 9.680149469251924E-6 5.808089681551155E-5 0.0 1.4520224203877885E-5 0.0 20-21 9.680149469251924E-6 5.808089681551155E-5 0.0 3.87205978770077E-5 0.0 22-23 9.680149469251924E-6 5.808089681551155E-5 0.0 8.712134522326733E-5 0.0 24-25 9.680149469251924E-6 8.712134522326733E-5 9.680149469251924E-6 2.662041104044279E-4 0.0 26-27 9.680149469251924E-6 1.0648164416177118E-4 9.680149469251924E-6 6.34049790236001E-4 9.680149469251924E-6 28-29 1.4520224203877885E-5 1.161617936310231E-4 2.9040448407755774E-5 0.0017956677265462322 1.936029893850385E-5 30-31 2.9040448407755774E-5 1.2100186836564906E-4 2.9040448407755774E-5 0.004970756752460863 1.936029893850385E-5 32-33 2.9040448407755774E-5 1.2584194310027503E-4 2.9040448407755774E-5 0.012409951619580969 1.936029893850385E-5 34-35 2.9040448407755774E-5 1.2584194310027503E-4 2.9040448407755774E-5 0.023730886423871094 1.936029893850385E-5 36-37 2.9040448407755774E-5 1.645625409772827E-4 2.9040448407755774E-5 0.04341547036959488 1.936029893850385E-5 38-39 2.9040448407755774E-5 2.032831388542904E-4 2.9040448407755774E-5 0.07492919696674452 1.936029893850385E-5 40-41 2.9040448407755774E-5 2.1296328832354233E-4 2.9040448407755774E-5 0.11094903314183094 1.936029893850385E-5 42-43 2.9040448407755774E-5 2.5168388620055006E-4 2.9040448407755774E-5 0.1478352426944154 1.936029893850385E-5 44-45 3.87205978770077E-5 2.56523960935176E-4 2.9040448407755774E-5 0.19279469690435597 1.936029893850385E-5 46-47 4.840074734625962E-5 2.710441851390539E-4 5.808089681551155E-5 0.24829099381157724 1.936029893850385E-5 48-49 4.840074734625962E-5 2.7588425987367987E-4 5.808089681551155E-5 0.30943081785937243 1.936029893850385E-5 50-51 4.840074734625962E-5 2.8072433460830584E-4 5.808089681551155E-5 0.3733198043564351 1.936029893850385E-5 52-53 4.840074734625962E-5 2.8072433460830584E-4 6.292097155013751E-5 0.4398369514343997 1.936029893850385E-5 54-55 4.840074734625962E-5 2.9040448407755774E-4 6.776104628476347E-5 0.5137545727816074 1.936029893850385E-5 56-57 4.840074734625962E-5 2.9040448407755774E-4 6.776104628476347E-5 0.600353189933535 2.420037367312981E-5 58-59 4.840074734625962E-5 2.9040448407755774E-4 6.776104628476347E-5 0.6976193318005784 2.9040448407755774E-5 60-61 4.840074734625962E-5 2.9040448407755774E-4 6.776104628476347E-5 0.8019471427054411 2.9040448407755774E-5 62-63 4.840074734625962E-5 2.9040448407755774E-4 6.776104628476347E-5 0.9079931801410959 2.9040448407755774E-5 64-65 4.840074734625962E-5 2.9040448407755774E-4 6.776104628476347E-5 1.021192848034528 2.9040448407755774E-5 66-67 4.840074734625962E-5 2.9040448407755774E-4 6.776104628476347E-5 1.1516473823569013 2.9040448407755774E-5 68-69 4.840074734625962E-5 2.952445588121837E-4 6.776104628476347E-5 1.2981661247234986 3.87205978770077E-5 70-71 4.840074734625962E-5 3.049247082814356E-4 6.776104628476347E-5 1.4547425423886486 3.87205978770077E-5 72-73 4.840074734625962E-5 3.2912508195456547E-4 6.776104628476347E-5 1.6198713721098823 3.87205978770077E-5 74-75 7.260112101938943E-5 3.3880523142381736E-4 6.776104628476347E-5 1.7920376704952625 3.87205978770077E-5 76-77 7.74411957540154E-5 3.484853808930693E-4 6.776104628476347E-5 1.9763670766887578 3.87205978770077E-5 78-79 7.74411957540154E-5 3.484853808930693E-4 6.776104628476347E-5 2.181876649920976 3.87205978770077E-5 80-81 7.74411957540154E-5 3.726857545661991E-4 6.776104628476347E-5 2.4051154169061295 3.87205978770077E-5 82-83 7.74411957540154E-5 3.87205978770077E-4 6.776104628476347E-5 2.6345301192526653 3.87205978770077E-5 84-85 7.74411957540154E-5 3.87205978770077E-4 6.776104628476347E-5 2.868334769383507 3.87205978770077E-5 86-87 7.74411957540154E-5 3.87205978770077E-4 6.776104628476347E-5 3.1136345970090855 3.87205978770077E-5 88 7.74411957540154E-5 3.87205978770077E-4 6.776104628476347E-5 3.310214232355919 3.87205978770077E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14620 0.0 49.878902 1 GTATCAA 24690 0.0 38.072113 1 ATCAACG 29935 0.0 30.994156 3 TCAACGC 30020 0.0 30.875086 4 CAACGCA 31175 0.0 29.866886 5 TATCAAC 32120 0.0 28.929642 2 AACGCAG 32760 0.0 28.485456 6 ACGCAGA 38345 0.0 24.31199 7 CGCAGAG 39185 0.0 23.730835 8 GTACATG 34140 0.0 22.655354 1 GCAGAGT 43990 0.0 21.042547 9 TACATGG 35375 0.0 21.032766 2 TACCTGG 8615 0.0 20.950186 2 GTGGTAT 6985 0.0 20.610456 1 ACATGGG 35115 0.0 20.412167 3 GTACCTG 9520 0.0 19.61942 1 GAGTACT 25615 0.0 19.387312 12-13 AGAGTAC 40470 0.0 19.323961 10-11 ATAACGC 1050 0.0 19.248245 3 CAGAGTA 42655 0.0 19.039461 10-11 >>END_MODULE