FastQCFastQC Report
Wed 25 May 2016
SRR2049479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4481873
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50450.038.5927771
GTATCAA77750.032.5423361
TCAACGC81000.030.4034064
ATCAACG81150.030.2913193
CAACGCA88400.027.9655885
TATCAAC91100.027.0860652
AACGCAG96100.025.822956
GTACCTG32950.023.83571
TACCTGG33800.023.2222772
ACGCAGA107050.022.830837
GTACATG117550.022.804421
CGCAGAG106700.022.6391498
ATAACGC3150.022.3813233
TACATGG120700.021.0276892
GTGGTAT30750.020.341051
ACATGGG122600.020.241773
GCAGAGT122600.018.7837189
TATAACG4101.8189894E-1218.3417662
TCTGTCG14700.017.5835598
AGAGTAC107900.017.18319310-11
Produced by FastQC (version 0.11.5)