##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049479_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4481873
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.775936979918885	31.0	31.0	34.0	28.0	34.0
2	30.95456319266521	31.0	31.0	34.0	28.0	34.0
3	31.023531902845082	31.0	31.0	34.0	28.0	34.0
4	34.692242729769454	37.0	35.0	37.0	32.0	37.0
5	34.72825758338087	37.0	35.0	37.0	32.0	37.0
6	34.83933592049574	37.0	35.0	37.0	32.0	37.0
7	34.774855289295346	37.0	35.0	37.0	32.0	37.0
8	34.720488733170264	37.0	35.0	37.0	32.0	37.0
9	36.32459621234247	39.0	37.0	39.0	32.0	39.0
10-11	36.259611439235336	39.0	37.0	39.0	32.0	39.0
12-13	36.17824623767787	39.0	37.0	39.0	32.0	39.0
14-15	37.42095034821379	40.0	38.0	41.0	32.0	41.0
16-17	37.40285835854787	40.0	38.0	41.0	32.0	41.0
18-19	37.41516615932669	40.0	38.0	41.0	32.0	41.0
20-21	37.46509059939896	40.0	38.0	41.0	32.0	41.0
22-23	37.43180005323667	40.0	38.0	41.0	32.0	41.0
24-25	37.43326528886472	40.0	38.0	41.0	32.0	41.0
26-27	37.43486651674423	40.0	38.0	41.0	32.0	41.0
28-29	37.41607493117275	40.0	38.0	41.0	32.0	41.0
30-31	37.363933672373136	40.0	38.0	41.0	32.0	41.0
32-33	37.32037855601888	40.0	38.0	41.0	31.5	41.0
34-35	37.26120073014117	40.0	38.0	41.0	31.0	41.0
36-37	37.155555724135866	40.0	37.5	41.0	31.0	41.0
38-39	37.04898431972526	40.0	37.0	41.0	30.5	41.0
40-41	36.92874184520623	40.0	37.0	41.0	30.0	41.0
42-43	36.83683774618335	40.0	37.0	41.0	30.0	41.0
44-45	36.68419096212678	40.0	37.0	41.0	30.0	41.0
46-47	36.56354430391044	40.0	36.5	41.0	30.0	41.0
48-49	36.46463632503643	40.0	36.0	41.0	30.0	41.0
50-51	36.35206943614868	40.0	36.0	41.0	30.0	41.0
52-53	36.242519924147786	40.0	36.0	41.0	29.5	41.0
54-55	36.099308257953766	39.5	35.0	41.0	29.0	41.0
56-57	35.94308027023523	39.0	35.0	41.0	29.0	41.0
58-59	35.769662371066744	39.0	35.0	41.0	28.5	41.0
60-61	35.574885098261376	39.0	35.0	41.0	28.0	41.0
62-63	35.3221272668815	38.5	35.0	40.5	28.0	41.0
64-65	35.08495934623761	38.0	34.5	40.0	28.0	41.0
66-67	34.85786243831541	37.5	34.0	40.0	28.0	41.0
68-69	34.57284956534913	37.0	34.0	40.0	27.5	41.0
70-71	34.25183712256015	36.5	34.0	39.5	26.5	41.0
72-73	33.916563343048765	36.0	34.0	39.0	26.0	41.0
74-75	33.617171660151904	36.0	34.0	39.0	26.0	40.5
76-77	33.29319728604537	35.0	33.5	38.5	26.0	40.0
78-79	32.945398274337535	35.0	33.0	37.0	26.0	39.0
80-81	32.61609677471897	35.0	33.0	37.0	25.5	39.0
82-83	32.32253981761643	35.0	33.0	36.5	25.0	39.0
84-85	32.038541252730724	35.0	33.0	36.0	25.0	37.5
86-87	31.770213145263153	35.0	33.0	36.0	25.0	37.0
88-89	31.54484163205874	35.0	33.0	35.0	24.0	37.0
90-91	31.346507475780776	35.0	33.0	35.0	24.0	36.0
92-93	31.14434824458435	35.0	32.0	35.0	23.0	36.0
94-95	30.949480719333188	35.0	32.0	35.0	20.5	36.0
96-97	30.766583635904006	35.0	32.0	35.0	19.5	36.0
98-99	30.60040433988201	35.0	32.0	35.0	17.5	35.0
100	30.478576032832702	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12421132821436132
1101	2	0.1420089283551249
1101	3	0.1386158726805995
1101	4	0.24080167974104683
1101	5	0.25915007737666684
1101	6	0.2835464212225389
1101	7	0.3459661234095961
1101	8	0.3670028102408125
1101	9	0.4182398152046858
1101	10-11	0.5022873380279691
1101	12-13	0.4678595574877633
1101	14-15	0.6417652153210724
1101	16-17	0.5427799146311116
1101	18-19	0.554873996844826
1101	20-21	0.5770816454741521
1101	22-23	0.5128656289012952
1101	24-25	0.5015711762246866
1101	26-27	0.5525512864475175
1101	28-29	0.5465172486372154
1101	30-31	0.5344539827623223
1101	32-33	0.48319668461544296
1101	34-35	0.5292457381823539
1101	36-37	0.5347276960691971
1101	38-39	0.4933859747904563
1101	40-41	0.5848373693007076
1101	42-43	0.5130553919081393
1101	44-45	0.5317713324985647
1101	46-47	0.4304238879527773
1101	48-49	0.42335674571182835
1101	50-51	0.4426203652557561
1101	52-53	0.4236134888150005
1101	54-55	0.41874828982010825
1101	56-57	0.4424982361949503
1101	58-59	0.35941981330292094
1101	60-61	0.34761282604020494
1101	62-63	0.37566442764824615
1101	64-65	0.38292369354693534
1101	66-67	0.33679388661725085
1101	68-69	0.2936470491842229
1101	70-71	0.3575250751136423
1101	72-73	0.3727094071667665
1101	74-75	0.3047828354983366
1101	76-77	0.299859202354277
1101	78-79	0.27367737295481476
1101	80-81	0.3593473253583497
1101	82-83	0.3083820961063921
1101	84-85	0.2734694806717215
1101	86-87	0.24765216228427178
1101	88-89	0.23943989465088933
1101	90-91	0.2794745840294901
1101	92-93	0.19901865972446942
1101	94-95	0.2551012481683159
1101	96-97	0.25602569993149515
1101	98-99	0.331171788877338
1101	100	0.2743206420302826
1102	1	0.05014989063635866
1102	2	0.11552032720178929
1102	3	0.12880326240388484
1102	4	0.22703067337189253
1102	5	0.20645784623668106
1102	6	0.21681071953987896
1102	7	0.26287154364456455
1102	8	0.3477209268221273
1102	9	0.46499431772221556
1102	10-11	0.41887504029160283
1102	12-13	0.4195937932970324
1102	14-15	0.4563492434873595
1102	16-17	0.45025136372092334
1102	18-19	0.4793723050386518
1102	20-21	0.485573826586176
1102	22-23	0.5007838062692898
1102	24-25	0.4740388463197931
1102	26-27	0.4432025107880051
1102	28-29	0.5084043387305996
1102	30-31	0.4284518364009102
1102	32-33	0.4176485421056526
1102	34-35	0.40954782913643584
1102	36-37	0.4229686345509265
1102	38-39	0.4279097811122696
1102	40-41	0.4245174188983398
1102	42-43	0.4220812047282152
1102	44-45	0.3005606017674012
1102	46-47	0.2695153692111134
1102	48-49	0.2225251651506568
1102	50-51	0.276858460750006
1102	52-53	0.2564321750696834
1102	54-55	0.2884073330485819
1102	56-57	0.23407934875251613
1102	58-59	0.26096516661875313
1102	60-61	0.21666938185635587
1102	62-63	0.2047996026923613
1102	64-65	0.24937603786849394
1102	66-67	0.26671861603585256
1102	68-69	0.23345445493797712
1102	70-71	0.1392147026473225
1102	72-73	0.2012549380012132
1102	74-75	0.2318478342072936
1102	76-77	0.2443627823129617
1102	78-79	0.24664471759913198
1102	80-81	0.2630107984831014
1102	82-83	0.21905150265629203
1102	84-85	0.22628812658125952
1102	86-87	0.24757174097343082
1102	88-89	0.226422938933716
1102	90-91	0.23536974853006853
1102	92-93	0.2267147236526874
1102	94-95	0.2646319804056958
1102	96-97	0.3034567231918146
1102	98-99	0.2515273909343989
1102	100	0.22087597404388148
1103	1	0.11136474466852064
1103	2	0.1721225053376898
1103	3	0.1640515515819807
1103	4	0.2293636388982776
1103	5	0.21748075439685977
1103	6	0.30050275666001625
1103	7	0.36511274144395856
1103	8	0.30790659248235386
1103	9	0.3721003091221604
1103	10-11	0.39944275324890555
1103	12-13	0.4115055040409956
1103	14-15	0.4367793373268398
1103	16-17	0.4227204611174642
1103	18-19	0.3476167098375953
1103	20-21	0.3542398563558038
1103	22-23	0.43341811155252685
1103	24-25	0.45200829214793004
1103	26-27	0.4947908458304795
1103	28-29	0.4067442066448166
1103	30-31	0.43542449507368275
1103	32-33	0.44449121922938417
1103	34-35	0.4072675992040473
1103	36-37	0.40564596610389003
1103	38-39	0.4313154483701567
1103	40-41	0.4286174814406749
1103	42-43	0.41427614198129703
1103	44-45	0.3657845949039853
1103	46-47	0.34704500538097705
1103	48-49	0.31284591056697764
1103	50-51	0.28869402960924617
1103	52-53	0.3081365673861356
1103	54-55	0.2847529901879824
1103	56-57	0.278951104490595
1103	58-59	0.35113303076875013
1103	60-61	0.3285291103719672
1103	62-63	0.3757086487301393
1103	64-65	0.36737420251395747
1103	66-67	0.3373965866506978
1103	68-69	0.3122368212464224
1103	70-71	0.3593491784120033
1103	72-73	0.3257992654502857
1103	74-75	0.34064136461501704
1103	76-77	0.2631178845791027
1103	78-79	0.2688980341938958
1103	80-81	0.22454174135265959
1103	82-83	0.2638448694018294
1103	84-85	0.2442716785313408
1103	86-87	0.280736324735674
1103	88-89	0.27698117636987973
1103	90-91	0.21387499318880643
1103	92-93	0.179537019638353
1103	94-95	0.20912504002484056
1103	96-97	0.14721486165132092
1103	98-99	0.17606918551627615
1103	100	0.21368276108885098
1104	1	-0.012845382373278369
1104	2	0.05321391208405757
1104	3	0.039619144268375095
1104	4	0.1856923150893195
1104	5	0.21065349000759426
1104	6	0.2697347199560127
1104	7	0.32510885703019454
1104	8	0.28406527498002276
1104	9	0.3467187006367638
1104	10-11	0.3825459605678958
1104	12-13	0.3819996056672821
1104	14-15	0.46973625599464697
1104	16-17	0.39007161792130063
1104	18-19	0.38539022753826657
1104	20-21	0.4486495827896704
1104	22-23	0.4454305624066137
1104	24-25	0.3788627696443072
1104	26-27	0.41688075915055123
1104	28-29	0.40753689604978405
1104	30-31	0.36604138760929317
1104	32-33	0.402392848548466
1104	34-35	0.388525265769573
1104	36-37	0.4042091402079322
1104	38-39	0.42465106283000154
1104	40-41	0.44860367869214457
1104	42-43	0.4299680877332577
1104	44-45	0.38592592191992026
1104	46-47	0.3860325504549209
1104	48-49	0.37211110731784913
1104	50-51	0.362355137706146
1104	52-53	0.3713997118634964
1104	54-55	0.34375116434077313
1104	56-57	0.28857385116351963
1104	58-59	0.34206519505970334
1104	60-61	0.43252265296595027
1104	62-63	0.3621407789029121
1104	64-65	0.3833947316653834
1104	66-67	0.35983181547018717
1104	68-69	0.2754564820067955
1104	70-71	0.2997850002585807
1104	72-73	0.3531770776245651
1104	74-75	0.34530251306143356
1104	76-77	0.28207479855797146
1104	78-79	0.30205894628089425
1104	80-81	0.3745720898776099
1104	82-83	0.350215697898669
1104	84-85	0.302824366692505
1104	86-87	0.23983545909629456
1104	88-89	0.23264999579313184
1104	90-91	0.2301424565469219
1104	92-93	0.21736363337597453
1104	94-95	0.1993703738234487
1104	96-97	0.1870673674498704
1104	98-99	0.23386558522738454
1104	100	0.23928813056286202
1105	1	0.09079937996752463
1105	2	0.04829270760505722
1105	3	0.13474096824867843
1105	4	0.1853234174127678
1105	5	0.17456045586693847
1105	6	0.18126712248891863
1105	7	0.26108972140551145
1105	8	0.2924137275723879
1105	9	0.3328274440182568
1105	10-11	0.3186156403118474
1105	12-13	0.3156960460927891
1105	14-15	0.39627390642470317
1105	16-17	0.3572112159227032
1105	18-19	0.3661621503678347
1105	20-21	0.34794950786018575
1105	22-23	0.3181520052646647
1105	24-25	0.3472261327513806
1105	26-27	0.32029045065380757
1105	28-29	0.27929807900183334
1105	30-31	0.3289462366643434
1105	32-33	0.3102522752465191
1105	34-35	0.2937422341888265
1105	36-37	0.29916461452724974
1105	38-39	0.32328072535776187
1105	40-41	0.31644921903109235
1105	42-43	0.29844472254523424
1105	44-45	0.2424949353745518
1105	46-47	0.19040297480992763
1105	48-49	0.19456331561073625
1105	50-51	0.19915429904131088
1105	52-53	0.15662154754260627
1105	54-55	0.1292670186223006
1105	56-57	0.09890918961873751
1105	58-59	0.11554885359605294
1105	60-61	0.10326246277983131
1105	62-63	0.14028320504782243
1105	64-65	0.1432995658598486
1105	66-67	0.15197140686955635
1105	68-69	0.16456836361134464
1105	70-71	0.11274083022348691
1105	72-73	0.12609424559570925
1105	74-75	0.10416232923150659
1105	76-77	0.11476969115451396
1105	78-79	0.1388665342127382
1105	80-81	0.15604130517925086
1105	82-83	0.11137866378893335
1105	84-85	0.07964884670966654
1105	86-87	0.1229031301652661
1105	88-89	0.08544697888907038
1105	90-91	0.10684232136366845
1105	92-93	0.10534057724718338
1105	94-95	0.09821071327173314
1105	96-97	0.13242774214005237
1105	98-99	0.13847484237919616
1105	100	0.10625507215984697
1106	1	0.03359104250809963
1106	2	0.04421368818693239
1106	3	0.02073653753643967
1106	4	0.07240733308197633
1106	5	0.1112746440628598
1106	6	0.1651375053897297
1106	7	0.17976444993561813
1106	8	0.13045858314425374
1106	9	0.1758505660010954
1106	10-11	0.2022254378466215
1106	12-13	0.15781218134328867
1106	14-15	0.21572186438102392
1106	16-17	0.18112639365551075
1106	18-19	0.18612278065734245
1106	20-21	0.21282237386262892
1106	22-23	0.24740188031559285
1106	24-25	0.27800035950840396
1106	26-27	0.2746676432413082
1106	28-29	0.196566460347114
1106	30-31	0.24568044640006548
1106	32-33	0.2561255674883185
1106	34-35	0.28587958951960957
1106	36-37	0.26964028854959565
1106	38-39	0.27909879355695466
1106	40-41	0.3484587317717214
1106	42-43	0.31455550274419863
1106	44-45	0.3047293913329483
1106	46-47	0.27215617023265537
1106	48-49	0.26762407675957434
1106	50-51	0.3559882215961778
1106	52-53	0.2961407539829892
1106	54-55	0.28658641250810035
1106	56-57	0.27333640000763637
1106	58-59	0.3467958123319761
1106	60-61	0.29425548407937896
1106	62-63	0.4121612986611609
1106	64-65	0.3642517050778977
1106	66-67	0.4086605739131315
1106	68-69	0.33486315391375854
1106	70-71	0.33496223959635785
1106	72-73	0.34090266913705136
1106	74-75	0.20622548461044943
1106	76-77	0.1892206838662247
1106	78-79	0.16695402526461578
1106	80-81	0.21758191764214985
1106	82-83	0.23355622406236165
1106	84-85	0.2017481967388406
1106	86-87	0.16970447489611473
1106	88-89	0.22527715321886888
1106	90-91	0.23950197457762812
1106	92-93	0.11837102359171325
1106	94-95	0.16200049167462538
1106	96-97	0.22439413051301926
1106	98-99	0.20394546365927724
1106	100	0.24810159588236047
1107	1	0.12051885595963086
1107	2	0.127148837755918
1107	3	0.09943812328734225
1107	4	0.16480779332189854
1107	5	0.12785935780614466
1107	6	0.2156028110779431
1107	7	0.21555231046730228
1107	8	0.17086017343876136
1107	9	0.2147232789846356
1107	10-11	0.22725755309799212
1107	12-13	0.2331882422284508
1107	14-15	0.28056798385915727
1107	16-17	0.33358807906454047
1107	18-19	0.36364557895354466
1107	20-21	0.33374413055101115
1107	22-23	0.2976538503175874
1107	24-25	0.3032759001857599
1107	26-27	0.4019994245887162
1107	28-29	0.32154001379795005
1107	30-31	0.34675858282201233
1107	32-33	0.28548845518778165
1107	34-35	0.30029425853281566
1107	36-37	0.3345655173095494
1107	38-39	0.32278124621498705
1107	40-41	0.3374952478380848
1107	42-43	0.31652078935471195
1107	44-45	0.26030610539184806
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2216	94-95	-1.0304102743222643
2216	96-97	-1.1107317618481787
2216	98-99	-1.1842476140532057
2216	100	-1.1752458050998555
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74025.0
3	13434.0
4	8506.0
5	6851.0
6	5639.0
7	5503.0
8	5945.0
9	6722.0
10	7669.0
11	8712.0
12	9871.0
13	10775.0
14	12369.0
15	13212.0
16	13367.0
17	14170.0
18	13831.0
19	12203.0
20	12420.0
21	13243.0
22	15166.0
23	18650.0
24	22908.0
25	27361.0
26	33134.0
27	37961.0
28	45944.0
29	57707.0
30	69242.0
31	80314.0
32	103980.0
33	138570.0
34	192845.0
35	282756.0
36	474369.0
37	883038.0
38	1236987.0
39	491240.0
40	1234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.793029473494755	20.420559297239734	14.008334101866653	22.77807712739886
2	15.870714211595688	20.99222647328248	39.85760621792876	23.279453097193073
3	17.92866249486447	26.150977999065837	30.76535632265478	25.15500318341491
4	12.615183233221863	18.11005998654866	39.27387311557396	30.000883664655507
5	12.764451946004831	37.47672064190111	35.269348811698386	14.489478600395678
6	29.09316439777211	38.49450781660189	18.777360784246348	13.634967001379655
7	25.807123994714505	32.895635090932906	23.320838190014108	17.97640272433848
8	24.119287151159615	35.764478277702224	21.679700008144675	18.436534562993494
9	24.24223761455832	17.83890976382426	22.520273009790802	35.39857961182661
10-11	22.94487656227406	27.438818928843673	29.542455204842895	20.073849304039374
12-13	23.669409669528612	25.303015882384905	29.259804298418747	21.767770149667736
14-15	21.366469731166475	27.66281598686487	26.57391920612601	24.39679507584265
16-17	20.78120739232909	30.672290191181382	27.015143393850078	21.531359022639442
18-19	20.629271464541656	29.547466865886392	29.0601729248615	20.763088744710455
20-21	21.897119509156727	29.014759774996694	28.424815077430658	20.663305638415917
22-23	21.568140235840396	29.037360326391	28.408540383031433	20.985959054737172
24-25	21.328954098369437	28.97796502146325	28.47004830012309	21.223032580044222
26-27	21.135730344900836	28.79044616742636	28.607979318266814	21.465844169405994
28-29	21.205458879605295	28.866177339390326	28.624161729043156	21.304202051961223
30-31	21.53569437404543	29.346479821761033	28.141699931014852	20.97612587317868
32-33	21.039205053879336	29.00761161356835	28.664185249402518	21.288998083149792
34-35	21.161698554562655	28.554589431484818	28.745140278403504	21.53857173554903
36-37	21.694846987246752	29.007739198008665	28.134564866060497	21.16284894868409
38-39	21.456681337846454	29.08511088187199	28.288794428485115	21.169413351796443
40-41	21.254722548299952	28.79802449895326	28.52737719589221	21.41987575685458
42-43	21.16645438674159	28.840195166622635	28.425992702449893	21.56735774418588
44-45	21.437674258702767	29.051324101129993	28.210206975291264	21.300794664875973
46-47	21.406259937715284	29.235210169079238	28.01363970600542	21.344890187200054
48-49	21.29330438033977	28.894915741410536	28.300703193543754	21.51107668470594
50-51	21.32263626197404	28.78107543523935	28.28552866188313	21.610759640903478
52-53	21.340592536938583	28.851920212728572	28.18817548076306	21.619311769569787
54-55	21.48548441821915	29.265713967888395	28.00489675017927	21.24390486371318
56-57	21.224703125840367	29.010113728394877	28.039848344286767	21.725334801477995
58-59	21.15021392193941	28.59171868288792	28.423202860473808	21.834864534698863
60-61	21.56336490247713	29.090099304067117	27.938296190024918	21.408239603430832
62-63	21.34438531946513	29.068836806688765	28.001864685137452	21.584913188708658
64-65	21.347421527490905	28.725512316736417	28.27711870250959	21.649947453263085
66-67	21.248570162351008	28.838971829018416	28.057397911934945	21.85506009669563
68-69	21.697912685948914	29.032047509198073	27.940083514877305	21.329956289975705
70-71	21.723259490439734	28.745500291081328	28.035221677652842	21.496018540826096
72-73	21.370437134598358	28.89155573855277	28.36990209091056	21.36810503593831
74-75	21.356904642670003	28.930255061643095	28.18178754712164	21.53105274856526
76-77	21.519156110912515	28.989865315672947	27.88599071459588	21.60498785881866
78-79	21.595704336399034	29.103386441136742	28.030597431826727	21.2703117906375
80-81	21.406311492485635	28.894219562687617	28.14625893037568	21.553210014451064
82-83	21.33342735316616	28.97866791153686	28.252565536435213	21.435339198861765
84-85	21.41533646385237	28.873431546701557	28.077948562776236	21.633283426669838
86-87	21.6193155381372	29.045644403591353	27.973976015248052	21.361064043023394
88-89	21.337781700838917	28.943610905529766	28.09255434014301	21.626053053488306
90-91	21.2131801901046	28.87464854280623	28.091771681222593	21.820399585866575
92-93	21.440651189484285	29.106171965684872	27.922745888561966	21.530430956268876
94-95	21.31925492533956	28.91862281624618	28.07527511953581	21.68684713887845
96-97	21.30882100090499	28.96280494669517	28.104998276710237	21.6233757756896
98-99	21.265296108289146	29.010136208262153	28.04738107508363	21.67718660836507
100	21.45081015225708	28.99482658401697	27.927342345810004	21.62702091791595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3865.0
1	2815.0
2	2162.0
3	2501.0
4	2199.5
5	1831.5
6	1645.0
7	1595.0
8	1622.5
9	1642.5
10	1491.5
11	1248.5
12	1169.5
13	1221.5
14	1345.0
15	1504.5
16	1707.5
17	2059.5
18	2603.5
19	3432.0
20	4713.0
21	6692.5
22	9644.0
23	14363.0
24	20757.5
25	28496.0
26	38091.0
27	49617.0
28	63774.0
29	78984.0
30	92109.0
31	104285.5
32	115696.0
33	126139.5
34	135860.5
35	142780.0
36	147943.0
37	152601.5
38	156839.5
39	158731.0
40	158551.0
41	157115.0
42	153327.5
43	149307.0
44	145383.0
45	142291.0
46	140059.0
47	136550.5
48	133832.0
49	132543.0
50	129779.0
51	124326.0
52	116187.5
53	109629.0
54	105466.5
55	100620.5
56	93489.5
57	85479.0
58	78145.5
59	70824.0
60	62914.0
61	53982.0
62	45515.5
63	37627.0
64	30467.5
65	24396.0
66	19165.0
67	14851.5
68	11333.0
69	8539.5
70	6389.5
71	4808.0
72	3640.0
73	2630.5
74	1985.0
75	1434.0
76	962.5
77	701.0
78	482.0
79	319.5
80	228.0
81	156.0
82	114.0
83	89.0
84	65.5
85	51.0
86	44.0
87	32.5
88	22.5
89	26.0
90	24.5
91	16.5
92	13.5
93	13.0
94	12.0
95	9.0
96	5.5
97	5.0
98	7.5
99	8.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08134991776875428
2	0.01423512000451597
3	0.018652915867986442
4	0.011936973671498501
5	0.009705763639442706
6	0.007184496303219659
7	0.020794877498760005
8	0.009326457933993221
9	0.009415706335275453
10-11	0.013052578687526399
12-13	0.015919683578718095
14-15	0.010185473796334702
16-17	0.012918706085603051
18-19	0.008679407024697041
20-21	0.013509976744097835
22-23	0.013878126399387042
24-25	0.01617627273240451
26-27	0.005287967775972233
28-29	0.01228281122646715
30-31	0.010564779501784187
32-33	0.012561712480474123
34-35	0.008746343325658714
36-37	0.010386282699219724
38-39	0.019311122827442902
40-41	0.012929862135763328
42-43	0.008233165018285882
44-45	0.020694473047317495
46-47	0.018697540068627557
48-49	0.018217829911735563
50-51	0.010252410097296376
52-53	0.01074327630434865
54-55	0.011903505521017665
56-57	0.02083950169940112
58-59	0.0021865858314146784
60-61	0.007463397557226632
62-63	0.015339568970383586
64-65	0.02454331035261374
66-67	0.013275699690731979
68-69	0.014179339753714576
70-71	0.026027065023930842
72-73	0.02076140934827917
74-75	0.020460195993951637
76-77	0.009616515238160475
78-79	0.011524199815568178
80-81	0.019824301134815734
82-83	0.012249343075986314
84-85	0.011323390912683157
86-87	0.017314189848752964
88-89	0.015138760067498567
90-91	0.015808123077115304
92-93	0.007831547212515838
94-95	0.015272632669421912
96-97	0.013800034048265089
98-99	0.010341658498578607
100	0.006872126898731847
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4481873.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.48195381407034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.49899656698474	53.64747614598201
2	13.178380502349144	17.786057288075398
3	3.5391349675665795	7.164832272692678
4	1.3634714771322978	3.680388769865759
5	0.6944020526091307	2.34298036212825
6	0.4055549756507819	1.642058528155949
7	0.2600847024877612	1.2285716716717727
8	0.18457840558486552	0.996456915260211
9	0.1420703409887128	0.8628465770054586
>10	0.6932027301162822	8.01570898465624
>50	0.030296897777263927	1.3700909783102555
>100	0.009627806301967193	1.0762019003212844
>500	9.928728144177677E-5	0.039508253404386615
>1k	9.92871688391984E-5	0.14682135247026695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.2312100320557945E-5	0.0	0.0	0.0
4	0.0	2.2312100320557945E-5	0.0	2.2312100320557945E-5	0.0
5	0.0	2.2312100320557945E-5	0.0	2.2312100320557945E-5	0.0
6	0.0	2.2312100320557945E-5	0.0	2.2312100320557945E-5	0.0
7	0.0	2.2312100320557945E-5	0.0	2.2312100320557945E-5	0.0
8	0.0	4.462420064111589E-5	0.0	2.2312100320557945E-5	0.0
9	0.0	4.462420064111589E-5	0.0	2.2312100320557945E-5	0.0
10-11	0.0	6.693630096167383E-5	0.0	2.2312100320557945E-5	0.0
12-13	0.0	6.693630096167383E-5	0.0	2.2312100320557945E-5	0.0
14-15	0.0	8.924840128223178E-5	0.0	6.693630096167383E-5	0.0
16-17	0.0	1.227165517630687E-4	0.0	1.0040445144251074E-4	0.0
18-19	1.1156050160278972E-5	1.3387260192334767E-4	0.0	1.1156050160278972E-4	0.0
20-21	2.2312100320557945E-5	1.3387260192334767E-4	0.0	1.7849680256446356E-4	0.0
22-23	2.2312100320557945E-5	1.3387260192334767E-4	0.0	2.9005730416725327E-4	0.0
24-25	2.2312100320557945E-5	1.5618470224390563E-4	0.0	4.2392990609060096E-4	0.0
26-27	2.2312100320557945E-5	1.7849680256446356E-4	0.0	6.358948591359014E-4	0.0
28-29	2.2312100320557945E-5	2.0080890288502151E-4	0.0	0.0013610381195540348	0.0
30-31	2.2312100320557945E-5	2.0080890288502151E-4	0.0	0.0033579710982439706	0.0
32-33	2.2312100320557945E-5	2.0080890288502151E-4	0.0	0.007296056804822448	0.0
34-35	2.2312100320557945E-5	2.0080890288502151E-4	0.0	0.01365500539618146	0.0
36-37	2.2312100320557945E-5	2.0080890288502151E-4	0.0	0.02336076903562417	0.0
38-39	2.2312100320557945E-5	2.1196495304530048E-4	0.0	0.03585554521513662	0.0
40-41	2.2312100320557945E-5	2.9005730416725327E-4	0.0	0.04899737230394525	1.1156050160278972E-5
42-43	2.2312100320557945E-5	2.9005730416725327E-4	0.0	0.062429256696921134	2.2312100320557945E-5
44-45	2.2312100320557945E-5	3.235254546480902E-4	0.0	0.07930836058942323	2.2312100320557945E-5
46-47	2.2312100320557945E-5	3.9046175560976404E-4	0.0	0.09957890373065012	2.2312100320557945E-5
48-49	2.2312100320557945E-5	4.0161780577004303E-4	0.0	0.12056343408213485	2.2312100320557945E-5
50-51	2.2312100320557945E-5	4.0161780577004303E-4	0.0	0.1425520089480447	2.2312100320557945E-5
52-53	2.2312100320557945E-5	4.7971015689199587E-4	0.0	0.16497566977020545	2.2312100320557945E-5
54-55	2.2312100320557945E-5	4.908662070522748E-4	0.0	0.18931817121993416	2.2312100320557945E-5
56-57	2.2312100320557945E-5	5.020222572125537E-4	0.0	0.2180561564328128	2.2312100320557945E-5
58-59	2.2312100320557945E-5	5.131783073728328E-4	0.0	0.24953852998512005	2.2312100320557945E-5
60-61	2.2312100320557945E-5	5.131783073728328E-4	0.0	0.2828281836633925	2.2312100320557945E-5
62-63	2.2312100320557945E-5	5.131783073728328E-4	0.0	0.3177243085647451	2.2312100320557945E-5
64-65	2.2312100320557945E-5	5.131783073728328E-4	0.0	0.35627961791866924	2.2312100320557945E-5
66-67	2.2312100320557945E-5	5.466464578536697E-4	0.0	0.40011173899840535	2.2312100320557945E-5
68-69	2.2312100320557945E-5	5.578025080139487E-4	0.0	0.4487521176972217	2.2312100320557945E-5
70-71	2.2312100320557945E-5	5.578025080139487E-4	0.0	0.5023457826672019	2.2312100320557945E-5
72-73	3.346815048083692E-5	5.689585581742276E-4	0.0	0.5630681636896002	2.2312100320557945E-5
74-75	4.462420064111589E-5	5.912706584947855E-4	0.0	0.625240831232835	2.2312100320557945E-5
76-77	4.462420064111589E-5	6.247388089756225E-4	0.0	0.6933819856118191	2.2312100320557945E-5
78-79	4.462420064111589E-5	6.470509092961804E-4	0.0	0.7728576869536464	2.2312100320557945E-5
80-81	4.462420064111589E-5	6.693630096167384E-4	0.0	0.8589935502411603	2.2312100320557945E-5
82-83	4.462420064111589E-5	6.693630096167384E-4	0.0	0.9508524672608973	2.2312100320557945E-5
84-85	5.578025080139486E-5	6.805190597770174E-4	0.0	1.0482224730598122	2.2312100320557945E-5
86-87	6.693630096167383E-5	6.916751099372963E-4	0.0	1.1549524049432012	2.2312100320557945E-5
88	6.693630096167383E-5	7.139872102578542E-4	0.0	1.2447028284826456	2.2312100320557945E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5045	0.0	38.592777	1
GTATCAA	7775	0.0	32.542336	1
TCAACGC	8100	0.0	30.403406	4
ATCAACG	8115	0.0	30.291319	3
CAACGCA	8840	0.0	27.965588	5
TATCAAC	9110	0.0	27.086065	2
AACGCAG	9610	0.0	25.82295	6
GTACCTG	3295	0.0	23.8357	1
TACCTGG	3380	0.0	23.222277	2
ACGCAGA	10705	0.0	22.83083	7
GTACATG	11755	0.0	22.80442	1
CGCAGAG	10670	0.0	22.639149	8
ATAACGC	315	0.0	22.381323	3
TACATGG	12070	0.0	21.027689	2
GTGGTAT	3075	0.0	20.34105	1
ACATGGG	12260	0.0	20.24177	3
GCAGAGT	12260	0.0	18.783718	9
TATAACG	410	1.8189894E-12	18.341766	2
TCTGTCG	1470	0.0	17.583559	8
AGAGTAC	10790	0.0	17.183193	10-11
>>END_MODULE