FastQCFastQC Report
Wed 25 May 2016
SRR2049479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4481873
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173610.3873603736652065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163050.36379879572669727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92420.20620843116259652No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53280.11887887050793272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90300.058.53141
GTATCAA163450.040.024581
TATAACG4750.035.6276322
ATAACGC5250.033.129933
ATCAACG199600.032.2419243
TCAACGC201250.031.9542244
CAACGCA207900.030.999955
GTGGTAT37050.030.9954911
TATCAAC209450.030.8378722
AACGCAG218400.029.5678756
TGGTATC38350.029.1735742
ACGCAGA242850.026.532947
CGCAGAG246950.026.035338
GCAGAGT270550.023.4689589
TAACGCA7150.023.0112444
GTACCTG35250.022.4309181
TACCTGG35400.021.5124472
CAGAGTA266000.021.47629410-11
GAGTACT213200.021.39411712-13
AGAGTAC254250.021.25802410-11
Produced by FastQC (version 0.11.5)