##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049479_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4481873
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02785844221824	33.0	31.0	34.0	30.0	34.0
2	32.19035791509487	33.0	31.0	34.0	30.0	34.0
3	32.14715856518023	34.0	31.0	34.0	30.0	34.0
4	35.86630968793627	37.0	35.0	37.0	35.0	37.0
5	35.908442073213585	37.0	35.0	37.0	35.0	37.0
6	35.96356077024048	37.0	35.0	37.0	35.0	37.0
7	35.958925208277876	37.0	35.0	37.0	35.0	37.0
8	35.967260116473625	37.0	35.0	37.0	35.0	37.0
9	37.692774203999086	39.0	37.0	39.0	35.0	39.0
10-11	37.66695553399215	39.0	37.0	39.0	35.0	39.0
12-13	37.644637074723	39.0	37.0	39.0	35.0	39.0
14-15	38.96078704148913	40.0	38.0	41.0	36.0	41.0
16-17	38.96579521552708	40.0	38.0	41.0	36.0	41.0
18-19	39.04698493241553	40.0	38.5	41.0	36.0	41.0
20-21	39.06597029857829	40.0	39.0	41.0	36.0	41.0
22-23	39.02332495811461	40.0	39.0	41.0	35.0	41.0
24-25	38.996268635902894	40.0	39.0	41.0	35.0	41.0
26-27	38.88946451628593	40.0	38.0	41.0	35.0	41.0
28-29	38.80499157383531	40.0	38.0	41.0	35.0	41.0
30-31	38.67752555683751	40.0	38.0	41.0	35.0	41.0
32-33	38.51486420967306	40.0	38.0	41.0	34.5	41.0
34-35	38.37846041152884	40.0	38.0	41.0	34.0	41.0
36-37	38.23044227268376	40.0	38.0	41.0	34.0	41.0
38-39	38.11241996370714	40.0	38.0	41.0	34.0	41.0
40-41	38.00925360892644	40.0	38.0	41.0	33.5	41.0
42-43	37.86080395406117	40.0	38.0	41.0	33.0	41.0
44-45	37.67472204143223	40.0	37.5	41.0	33.0	41.0
46-47	37.402261063622284	40.0	37.0	41.0	33.0	41.0
48-49	37.13354461404863	40.0	37.0	41.0	32.0	41.0
50-51	36.970252392247616	40.0	37.0	41.0	31.0	41.0
52-53	36.856471278860425	40.0	37.0	41.0	31.0	41.0
54-55	36.71467710039977	40.0	36.0	41.0	31.0	41.0
56-57	36.56601001411687	40.0	36.0	41.0	31.0	41.0
58-59	36.39780890712432	39.0	35.5	41.0	31.0	41.0
60-61	36.21029890851436	39.0	35.0	41.0	30.5	41.0
62-63	35.98884863538079	39.0	35.0	41.0	30.0	41.0
64-65	35.76421754922551	38.5	35.0	41.0	30.0	41.0
66-67	35.50476419122094	38.0	35.0	40.0	29.5	41.0
68-69	35.212714304934565	37.0	35.0	40.0	29.0	41.0
70-71	34.92455910731964	37.0	35.0	40.0	29.0	41.0
72-73	34.592725965238195	36.0	34.0	39.0	29.0	41.0
74-75	34.27272102533918	36.0	34.0	39.0	29.0	41.0
76-77	33.95284828463457	35.5	34.0	38.5	29.0	40.0
78-79	33.64469765207537	35.0	34.0	37.0	28.0	39.5
80-81	33.34032512746345	35.0	34.0	37.0	28.0	39.0
82-83	33.06067652965624	35.0	34.0	36.5	28.0	39.0
84-85	32.77489054241385	35.0	34.0	36.0	27.0	37.5
86-87	32.524576109140085	35.0	34.0	36.0	27.0	37.0
88-89	32.30413679727204	35.0	34.0	35.5	27.0	37.0
90-91	32.11410251919231	35.0	33.0	35.0	26.0	36.0
92-93	31.94680460602074	35.0	33.0	35.0	26.0	36.0
94-95	31.78551054436393	35.0	33.0	35.0	26.0	36.0
96-97	31.638182072539763	35.0	33.0	35.0	25.5	36.0
98-99	31.50563057007639	35.0	33.0	35.0	25.0	35.5
100	31.400661732271306	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2567960545003345
1101	2	0.25649495824794855
1101	3	0.39824081973164027
1101	4	0.21119809915244048
1101	5	0.21756647429510423
1101	6	0.23261829328984618
1101	7	0.19836329398094676
1101	8	0.22372946536086147
1101	9	0.28504504452543955
1101	10-11	0.23526812934026964
1101	12-13	0.22944102946888734
1101	14-15	0.35798902385522524
1101	16-17	0.3051793308414332
1101	18-19	0.31936072529585857
1101	20-21	0.372676362969635
1101	22-23	0.29966752415389664
1101	24-25	0.31936688583040507
1101	26-27	0.36860122578748644
1101	28-29	0.37991022942188124
1101	30-31	0.3926315302883765
1101	32-33	0.4264746262231469
1101	34-35	0.38962870205780575
1101	36-37	0.37964190421664057
1101	38-39	0.37270160685670106
1101	40-41	0.3865648982701515
1101	42-43	0.3380013716779757
1101	44-45	0.3906477665977306
1101	46-47	0.45438936441714617
1101	48-49	0.4141357103986607
1101	50-51	0.37774194072435563
1101	52-53	0.3430769286318949
1101	54-55	0.3753293178056154
1101	56-57	0.3737356840148678
1101	58-59	0.286996839896851
1101	60-61	0.31589205166877576
1101	62-63	0.31690012589051975
1101	64-65	0.3170617326574714
1101	66-67	0.3657557247644263
1101	68-69	0.36732217856497584
1101	70-71	0.3831723428027942
1101	72-73	0.4240889855264314
1101	74-75	0.38554071639703835
1101	76-77	0.38895150954318325
1101	78-79	0.32736488407725517
1101	80-81	0.3434137071600176
1101	82-83	0.3196732966819411
1101	84-85	0.3116405668387543
1101	86-87	0.2531251025617891
1101	88-89	0.311679499535245
1101	90-91	0.32854729809486827
1101	92-93	0.31937426826263504
1101	94-95	0.3585563713270723
1101	96-97	0.36294081888251384
1101	98-99	0.3917878371745296
1101	100	0.3776158544182273
1102	1	0.26323392836529536
1102	2	0.2562800531397116
1102	3	0.33494373426449897
1102	4	0.21902330787129642
1102	5	0.20664141404636638
1102	6	0.21801025976572674
1102	7	0.19903651987069537
1102	8	0.19506800949306324
1102	9	0.257277502458912
1102	10-11	0.21662248876704382
1102	12-13	0.21232426794631465
1102	14-15	0.3055173461429561
1102	16-17	0.30163433794216843
1102	18-19	0.323847642712046
1102	20-21	0.2822033969025881
1102	22-23	0.24080161256892296
1102	24-25	0.24294102136855145
1102	26-27	0.25320309995368007
1102	28-29	0.26297145720516113
1102	30-31	0.2524144868877727
1102	32-33	0.32598490347493936
1102	34-35	0.355045521139445
1102	36-37	0.3408694185257133
1102	38-39	0.2977939299836052
1102	40-41	0.33704305541177604
1102	42-43	0.2544483366476342
1102	44-45	0.29703978148778276
1102	46-47	0.2886411772587323
1102	48-49	0.28267578119093173
1102	50-51	0.24693002169436085
1102	52-53	0.23018161350945832
1102	54-55	0.24157341396691123
1102	56-57	0.19054225865875196
1102	58-59	0.2055229243741934
1102	60-61	0.23338301435759234
1102	62-63	0.22724381268313465
1102	64-65	0.1361386627689356
1102	66-67	0.16970463974914196
1102	68-69	0.20187456269373172
1102	70-71	0.17844940632217998
1102	72-73	0.22941387148416936
1102	74-75	0.18585594641812264
1102	76-77	0.11736618764276585
1102	78-79	0.14772949654874168
1102	80-81	0.10247569612158713
1102	82-83	0.08173770366219202
1102	84-85	0.026211886286198194
1102	86-87	0.09925452438904614
1102	88-89	0.11416186870940948
1102	90-91	0.12482835040368911
1102	92-93	0.09910265133703433
1102	94-95	0.10183177800607623
1102	96-97	0.08771017916998858
1102	98-99	0.10553712441040375
1102	100	0.14793205272265553
1103	1	0.2615119683417291
1103	2	0.265038967695709
1103	3	0.3235408960244399
1103	4	0.16189819007673378
1103	5	0.18602920056073202
1103	6	0.1593830867767423
1103	7	0.16561670102915116
1103	8	0.13578242092292214
1103	9	0.24600416517257173
1103	10-11	0.20656557718943702
1103	12-13	0.18480771825697673
1103	14-15	0.2277458536282282
1103	16-17	0.2572810406361725
1103	18-19	0.22113792942611354
1103	20-21	0.19987677003874182
1103	22-23	0.28791222807513606
1103	24-25	0.2616612480017153
1103	26-27	0.22589476493718053
1103	28-29	0.21192054462609633
1103	30-31	0.2190840327966299
1103	32-33	0.2315178544535712
1103	34-35	0.2201637183614693
1103	36-37	0.21352296061060372
1103	38-39	0.23920338615103987
1103	40-41	0.18552050997116254
1103	42-43	0.23225184720157444
1103	44-45	0.20639151685900714
1103	46-47	0.2063885231061633
1103	48-49	0.19577122417317128
1103	50-51	0.16958716824410658
1103	52-53	0.1726637064630907
1103	54-55	0.21058527752720124
1103	56-57	0.18427023002046639
1103	58-59	0.09592322779869278
1103	60-61	0.09977180167923194
1103	62-63	0.1129927328049476
1103	64-65	0.11689569131304012
1103	66-67	0.1331073065335744
1103	68-69	0.07753156571433806
1103	70-71	0.0617409906435995
1103	72-73	0.11769280121139047
1103	74-75	0.14698150064518956
1103	76-77	0.12386578465710585
1103	78-79	0.15377525571723538
1103	80-81	0.1283236058891717
1103	82-83	0.135990679943518
1103	84-85	0.13271070395677498
1103	86-87	0.13375600741526483
1103	88-89	0.11485041891570091
1103	90-91	0.13669710631776866
1103	92-93	0.10799743148741925
1103	94-95	0.10756469560281445
1103	96-97	0.0827519942096373
1103	98-99	0.14069981566623113
1103	100	0.10342528964250874
1104	1	0.15963114951026824
1104	2	0.1798436802743879
1104	3	0.3279240398897727
1104	4	0.15758333193495844
1104	5	0.16241208826205877
1104	6	0.12266849136348412
1104	7	0.15237776010197734
1104	8	0.11636563332076832
1104	9	0.1937067034790232
1104	10-11	0.17280354343476745
1104	12-13	0.17826443067327347
1104	14-15	0.2581729676260238
1104	16-17	0.27219628872472157
1104	18-19	0.22819442111688915
1104	20-21	0.22933780269310944
1104	22-23	0.22346067262531477
1104	24-25	0.22564147895158015
1104	26-27	0.21293440853851564
1104	28-29	0.21792140862435616
1104	30-31	0.21197941780953755
1104	32-33	0.2630218567694129
1104	34-35	0.2512479744795755
1104	36-37	0.21701082607464883
1104	38-39	0.15822024448210215
1104	40-41	0.23677059896841968
1104	42-43	0.26275615590239454
1104	44-45	0.24761361167103502
1104	46-47	0.2194023087030601
1104	48-49	0.1712041950229093
1104	50-51	0.18242862308292018
1104	52-53	0.2271658691116798
1104	54-55	0.2182752568872388
1104	56-57	0.22653613146913898
1104	58-59	0.26049807760156796
1104	60-61	0.20571088276859228
1104	62-63	0.21060321371339086
1104	64-65	0.20091839909247255
1104	66-67	0.26653336564403673
1104	68-69	0.25682596220070053
1104	70-71	0.23635181816317186
1104	72-73	0.2524141543693901
1104	74-75	0.25266233238356506
1104	76-77	0.24620002859014534
1104	78-79	0.27142298158917555
1104	80-81	0.21264990997188704
1104	82-83	0.2813081042909147
1104	84-85	0.25289532960010064
1104	86-87	0.2003567986362924
1104	88-89	0.17123096955233308
1104	90-91	0.15306773861962597
1104	92-93	0.18212064662211702
1104	94-95	0.1360485168590735
1104	96-97	0.11477231013784106
1104	98-99	0.08440618310635628
1104	100	0.08917904174805713
1105	1	0.1307291232930865
1105	2	0.1638891318483786
1105	3	0.25326823455404224
1105	4	0.1031503262954061
1105	5	0.1908784522290432
1105	6	0.10530170775437853
1105	7	0.10722992770713091
1105	8	0.0952859135629609
1105	9	0.11370380242505718
1105	10-11	0.12884132839027984
1105	12-13	0.1518328977435388
1105	14-15	0.19247499466426632
1105	16-17	0.20099161886329142
1105	18-19	0.17432711379840526
1105	20-21	0.20029984658886235
1105	22-23	0.1998890209737212
1105	24-25	0.176140769208871
1105	26-27	0.15535584558918458
1105	28-29	0.20526947390536776
1105	30-31	0.20066719192450932
1105	32-33	0.14727772598401856
1105	34-35	0.1957718233926613
1105	36-37	0.1554387285096439
1105	38-39	0.16304695584286577
1105	40-41	0.1610003222060925
1105	42-43	0.22533659298925102
1105	44-45	0.20087177207967954
1105	46-47	0.14264636020401866
1105	48-49	0.08172036440154073
1105	50-51	0.023735185382712132
1105	52-53	0.013954703664630586
1105	54-55	0.10106530640658207
1105	56-57	0.04816194510686245
1105	58-59	0.09706028591918425
1105	60-61	0.07771636500194745
1105	62-63	0.07456351680870199
1105	64-65	0.0508951971427436
1105	66-67	0.020420349425862128
1105	68-69	0.0540963186555814
1105	70-71	0.03196861707098719
1105	72-73	0.07507300633993452
1105	74-75	0.11421459743714735
1105	76-77	0.05619216928828763
1105	78-79	0.054055727435070366
1105	80-81	0.0329721699169383
1105	82-83	-0.025103068924934746
1105	84-85	-0.015966052572544243
1105	86-87	0.04916825358376542
1105	88-89	0.06523164564867301
1105	90-91	0.09475753821475053
1105	92-93	0.0823641944718787
1105	94-95	0.05698003174724775
1105	96-97	0.10517837774838767
1105	98-99	0.10587735549106725
1105	100	0.09243612542490709
1106	1	0.10517050082157908
1106	2	0.109022672276879
1106	3	0.19629564696781898
1106	4	0.1102537107668553
1106	5	0.0953247122726566
1106	6	0.0740132277310721
1106	7	0.07768695331605358
1106	8	0.08172245009055956
1106	9	0.1182143388257586
1106	10-11	0.10686586367075535
1106	12-13	0.09290040078271034
1106	14-15	0.1152124502841616
1106	16-17	0.1119657071343525
1106	18-19	0.15544663778251788
1106	20-21	0.1748129786885002
1106	22-23	0.12894786464627117
1106	24-25	0.1578185086507915
1106	26-27	0.16861682307846593
1106	28-29	0.1683836307747839
1106	30-31	0.2226267884110058
1106	32-33	0.17096337862041366
1106	34-35	0.17106935511787924
1106	36-37	0.17955519130308772
1106	38-39	0.13549666396986737
1106	40-41	0.12586885536806847
1106	42-43	0.0833918205188624
1106	44-45	0.07888314626890036
1106	46-47	0.04569888989840365
1106	48-49	0.09190101981681664
1106	50-51	0.10929409435416204
1106	52-53	0.04272637826317549
1106	54-55	0.07976417679807923
1106	56-57	0.10645576630437148
1106	58-59	0.028464294218352393
1106	60-61	0.08519929121909087
1106	62-63	0.03988506622962973
1106	64-65	0.002490477612170139
1106	66-67	-0.05187454270655678
1106	68-69	0.010337751782969917
1106	70-71	0.0661646003276104
1106	72-73	0.09235524382158644
1106	74-75	0.12456162780041069
1106	76-77	0.133490125627155
1106	78-79	0.03162590348811278
1106	80-81	0.020261597463417047
1106	82-83	0.07269498343618608
1106	84-85	0.07654651166347293
1106	86-87	0.031026775433936393
1106	88-89	-0.010645025925960283
1106	90-91	-0.00807899031157433
1106	92-93	0.014452833973869872
1106	94-95	-0.005600427085713733
1106	96-97	-0.008812010696768624
1106	98-99	0.00950539430775521
1106	100	0.013904581839771168
1107	1	0.06896600262276564
1107	2	0.09495794295564508
1107	3	0.15589617262476452
1107	4	0.0504952828013856
1107	5	0.10142181501408487
1107	6	0.06801273068725067
1107	7	0.08429297651066037
1107	8	0.07481163887777598
1107	9	0.07993757333103702
1107	10-11	0.09475291754941395
1107	12-13	0.09012942583942163
1107	14-15	0.11578536120472194
1107	16-17	0.13855043020601698
1107	18-19	0.10892385020257933
1107	20-21	0.0873185339142708
1107	22-23	0.08928188601269227
1107	24-25	0.08467814387122985
1107	26-27	0.08851051858745507
1107	28-29	0.12470504598500298
1107	30-31	0.09268566350887397
1107	32-33	0.08685503635439318
1107	34-35	0.03908319726275522
1107	36-37	0.07371648263777075
1107	38-39	0.05915550169957129
1107	40-41	0.1126420769512606
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2216	52-53	-0.7257878336110011
2216	54-55	-0.681482142493536
2216	56-57	-0.6113450655546373
2216	58-59	-0.5790548822417136
2216	60-61	-0.664683334188112
2216	62-63	-0.6523143638379665
2216	64-65	-0.6691771832895839
2216	66-67	-0.5774336092075032
2216	68-69	-0.618272801959499
2216	70-71	-0.6389014632489136
2216	72-73	-0.6364036730259599
2216	74-75	-0.6309678903244063
2216	76-77	-0.6107089594262263
2216	78-79	-0.6065558127208774
2216	80-81	-0.590640794530195
2216	82-83	-0.5845555415536268
2216	84-85	-0.6183096879634036
2216	86-87	-0.5734237459039946
2216	88-89	-0.5843645439876326
2216	90-91	-0.5682662352518086
2216	92-93	-0.5581457652397219
2216	94-95	-0.5804825090366386
2216	96-97	-0.6225351202636098
2216	98-99	-0.6654778468993818
2216	100	-0.739124917740348
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	321.0
3	43.0
4	37.0
5	109.0
6	346.0
7	955.0
8	2144.0
9	3491.0
10	4549.0
11	5906.0
12	9307.0
13	13361.0
14	14415.0
15	17287.0
16	21722.0
17	30607.0
18	31953.0
19	16980.0
20	11647.0
21	10735.0
22	11085.0
23	12162.0
24	13798.0
25	16912.0
26	21357.0
27	27407.0
28	35763.0
29	46675.0
30	60785.0
31	78380.0
32	102623.0
33	134514.0
34	187027.0
35	277360.0
36	453054.0
37	883789.0
38	1334960.0
39	586249.0
40	2058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40182897934936	20.407695457869288	14.42550937356385	22.764966189217503
2	15.796900090654065	21.12706004833247	39.658687338976364	23.417352522037106
3	17.500808255834112	26.200541603923185	31.34365922461435	24.954990915628354
4	12.498569236566945	18.201943696307325	38.81948462171954	30.480002445406196
5	12.17310968813076	37.1419740895585	35.534303831223966	15.150612391086774
6	28.759749577240346	38.67754934836221	18.316007569177177	14.246693505220264
7	25.14446527155053	33.24763999336884	23.404500752252464	18.20339398282816
8	24.253208424245845	35.17353124463812	21.547553890973706	19.025706440142322
9	24.237880011325622	16.862347505161345	22.79776780823553	36.1020046752775
10-11	22.93802167085056	27.027974688260915	29.470134472797422	20.56386916809111
12-13	24.165700366788617	24.847859365939197	29.647459890095057	21.33898037717713
14-15	21.515379842311464	26.82830370249224	27.80480392907162	23.85151252612468
16-17	20.101952911204755	29.88971351932551	28.250588537426207	21.757745032043523
18-19	19.846724795637897	27.879393280443242	31.747363211764366	20.526518712154495
20-21	21.107503492401502	27.323197243652373	31.45379175179663	20.115507512149495
22-23	20.72545339861259	27.3570335437885	31.53776334135305	20.379749716245865
24-25	20.572570887216127	27.279699804077445	31.555345276405646	20.59238403230078
26-27	20.395937145028427	27.108599462769252	31.68170985657113	20.813753535631196
28-29	20.45703883175628	27.16347607350766	31.6854248212745	20.694060273461563
30-31	20.843517877458822	27.627021113717415	31.178928987947675	20.350532020876095
32-33	20.321370105757126	27.34534200322053	31.683093206791003	20.650194684231344
34-35	20.456481029248263	26.956732151937366	31.643656569474416	20.94313024933995
36-37	21.046848047680065	27.46928572050123	30.917051866485284	20.56681436533342
38-39	20.849028966238	27.57259074498541	30.982314759922918	20.596065528853675
40-41	20.630203042344128	27.328976077635396	31.136301720285246	20.904519159735226
42-43	20.62152363531943	27.48697921605543	30.833448426584155	21.058048722040986
44-45	20.966926104331826	27.668577400564452	30.522116534761246	20.842379960342473
46-47	21.004990547478698	27.986212460727916	29.98790907283629	21.020887918957097
48-49	21.028094727360635	27.777014208122363	29.95830091571091	21.23659014880609
50-51	21.08743375816316	27.7672861323826	29.74885723000183	21.396422879452405
52-53	21.088939824934798	27.928390652747186	29.579218331264627	21.40345119105338
54-55	21.28703780763087	28.351885472881538	29.340490013884818	21.02058670560277
56-57	20.942282389527772	28.072560288968475	29.39719621684952	21.587961104654237
58-59	20.84934133564249	27.611402643493022	29.834334886329888	21.7049211345346
60-61	21.219610640462147	28.090488061576043	29.386698373648695	21.303202924313116
62-63	21.044906894952177	28.02656389415764	29.48397913104633	21.444550079843854
64-65	21.04375782178567	27.716693444905733	29.737433434637705	21.50211529867089
66-67	20.97612984571406	27.834367461996358	29.530321363412128	21.65918132887746
68-69	21.508083785506642	28.006973870076195	29.265208987403255	21.219733357013908
70-71	21.477382335465553	27.76295758492041	29.387088835404306	21.37257124420973
72-73	21.177117245401643	27.963398338150142	29.6019766735916	21.257507742856614
74-75	21.21936520735862	27.942904674005714	29.38897420788139	21.448755910754276
76-77	21.32815900852166	28.0143591752823	29.10422718359043	21.553254632605608
78-79	21.43651772372845	28.108917856440822	29.24413520865049	21.210429211180237
80-81	21.246946724774883	27.896964825488478	29.377909989530032	21.4781784602066
82-83	21.101821792340218	27.978078248937383	29.52120218213461	21.398897776587795
84-85	21.226705942057716	27.970083379510253	29.23664557310124	21.566565105330792
86-87	21.369044796816492	28.09456368160675	29.155286965921977	21.381104555654783
88-89	21.085166329287198	27.971991461110623	29.279008266122396	21.663833943479784
90-91	20.95996467523844	27.917293826957756	29.261807979844	21.8609335179598
92-93	21.1723866800123	28.145274758326106	29.043795597637235	21.63854296402436
94-95	20.960644517261336	27.942145079750265	29.273644058077597	21.8235663449108
96-97	20.95119436741461	27.971142270618415	29.27104089505906	21.806622466907914
98-99	20.89118963583956	28.03462291459325	29.22994951057375	21.84423793899344
100	21.064185736840084	28.029763482547903	29.059601933836515	21.846448846775495
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1931.0
1	1407.0
2	2743.5
3	4059.5
4	2209.0
5	1018.5
6	2055.5
7	4730.0
8	7707.5
9	8895.0
10	6632.5
11	3804.0
12	3326.0
13	3664.0
14	4138.0
15	4632.5
16	5169.5
17	5876.0
18	6644.5
19	7553.0
20	8862.5
21	10662.0
22	13396.5
23	17807.0
24	23580.0
25	30562.5
26	39861.0
27	50913.0
28	63752.5
29	78402.0
30	91372.0
31	102938.0
32	114512.5
33	124216.5
34	132669.0
35	139385.0
36	144459.0
37	149677.0
38	154362.0
39	155859.5
40	155487.0
41	154448.0
42	151352.5
43	147657.5
44	144039.0
45	141656.5
46	139343.5
47	135450.5
48	132312.5
49	131116.5
50	128867.0
51	123180.5
52	114675.0
53	107932.5
54	103361.0
55	97743.5
56	90919.5
57	83226.0
58	76158.5
59	69919.5
60	61957.0
61	52579.0
62	44412.0
63	36540.5
64	29190.5
65	23321.0
66	18294.0
67	13996.5
68	10632.0
69	7958.5
70	5743.5
71	4064.0
72	3005.5
73	2224.5
74	1634.5
75	1155.5
76	698.0
77	474.0
78	302.5
79	155.0
80	85.5
81	48.5
82	30.5
83	15.5
84	7.0
85	2.5
86	2.0
87	0.5
88	0.0
89	1.0
90	1.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	3.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13240000330219084
2	0.0
3	0.0
4	0.0
5	0.014904483014132709
6	2.6774520384669533E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.227165517630687E-4
82-83	5.131783073728328E-4
84-85	3.681496552892061E-4
86-87	5.689585581742276E-4
88-89	5.689585581742276E-4
90-91	6.916751099372963E-4
92-93	1.5618470224390563E-4
94-95	1.5618470224390563E-4
96-97	5.243343575331117E-4
98-99	9.705763639442706E-4
100	1.1156050160278972E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4481873.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.82408557731836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.38993290343079	50.81555718932241
2	13.942160382678203	18.07575595558859
3	3.632389916706648	7.063990644323402
4	1.4372474774797783	3.7267301390373633
5	0.7412797840800882	2.4026392079971854
6	0.44065834479151383	1.7139164551874735
7	0.28723391710721013	1.3033773216286308
8	0.1951800507687181	1.012189465121338
9	0.1463878769172982	0.8540514234691996
>10	0.7390917511371793	8.308819925779959
>50	0.034317828983811034	1.5109631821962868
>100	0.013224632281212224	1.4850823355271416
>500	4.1313876993817107E-4	0.18802731371423895
>1k	3.442820483274959E-4	0.462379537385487
>5k	6.885640966530982E-5	0.32516989379753464
>10k+	6.885640966530982E-5	0.7513500099236651
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17361	0.3873603736652065	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16305	0.36379879572669727	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9242	0.20620843116259652	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5328	0.11887887050793272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.2312100320557945E-5	0.0	2.2312100320557945E-5	0.0
4	0.0	2.2312100320557945E-5	0.0	1.3387260192334767E-4	0.0
5	0.0	4.462420064111589E-5	0.0	1.3387260192334767E-4	0.0
6	0.0	4.462420064111589E-5	0.0	1.3387260192334767E-4	0.0
7	0.0	4.462420064111589E-5	0.0	1.3387260192334767E-4	0.0
8	0.0	4.462420064111589E-5	0.0	1.3387260192334767E-4	0.0
9	2.2312100320557945E-5	4.462420064111589E-5	0.0	1.7849680256446356E-4	0.0
10-11	2.2312100320557945E-5	5.578025080139486E-5	0.0	1.7849680256446356E-4	0.0
12-13	2.2312100320557945E-5	6.693630096167383E-5	0.0	1.8965285272474255E-4	0.0
14-15	2.2312100320557945E-5	8.924840128223178E-5	0.0	2.0080890288502151E-4	0.0
16-17	2.2312100320557945E-5	1.673407524041846E-4	0.0	2.2312100320557947E-4	0.0
18-19	3.346815048083692E-5	2.2312100320557945E-4	0.0	2.454331035261374E-4	0.0
20-21	4.462420064111589E-5	2.2312100320557945E-4	0.0	3.1236940448781125E-4	0.0
22-23	4.462420064111589E-5	2.342770533658584E-4	0.0	4.462420064111589E-4	0.0
24-25	4.462420064111589E-5	2.6774520384669533E-4	0.0	5.912706584947855E-4	0.0
26-27	4.462420064111589E-5	2.6774520384669533E-4	0.0	8.478598121812019E-4	0.0
28-29	5.578025080139486E-5	2.6774520384669533E-4	0.0	0.0015730030725993353	0.0
30-31	6.693630096167383E-5	2.6774520384669533E-4	0.0	0.0035476239509687132	0.0
32-33	6.693630096167383E-5	2.6774520384669533E-4	0.0	0.0075861141089897015	0.0
34-35	6.693630096167383E-5	2.6774520384669533E-4	0.0	0.014056623201951505	0.0
36-37	6.693630096167383E-5	3.0121335432753226E-4	0.0	0.023706606590592816	0.0
38-39	6.693630096167383E-5	3.569936051289271E-4	0.0	0.03624600697074638	0.0
40-41	6.693630096167383E-5	3.569936051289271E-4	0.0	0.04947708246083724	0.0
42-43	6.693630096167383E-5	3.569936051289271E-4	0.0	0.06296474710461453	0.0
44-45	6.693630096167383E-5	3.9046175560976404E-4	0.0	0.07975460259583438	0.0
46-47	6.693630096167383E-5	4.462420064111589E-4	0.0	0.1002705788405874	0.0
48-49	6.693630096167383E-5	4.462420064111589E-4	0.0	0.12127742129239272	0.0
50-51	6.693630096167383E-5	5.020222572125537E-4	0.0	0.1432994643087834	0.0
52-53	6.693630096167383E-5	5.578025080139487E-4	0.0	0.16582352958238666	0.0
54-55	6.693630096167383E-5	5.912706584947855E-4	0.0	0.19042262018580178	0.0
56-57	6.693630096167383E-5	6.135827588153436E-4	0.0	0.21940603850220658	0.0
58-59	6.693630096167383E-5	6.247388089756225E-4	0.0	0.25097766045579606	0.0
60-61	6.693630096167383E-5	6.470509092961804E-4	0.0	0.284367718585511	0.0
62-63	6.693630096167383E-5	6.582069594564593E-4	0.0	0.3192973116373445	0.0
64-65	6.693630096167383E-5	6.805190597770174E-4	0.0	0.3579753375430317	0.0
66-67	6.693630096167383E-5	7.028311600975753E-4	0.0	0.40191901912437056	0.0
68-69	6.693630096167383E-5	7.362993105784122E-4	0.0	0.4507490506759116	0.0
70-71	6.693630096167383E-5	7.362993105784122E-4	0.0	0.5041084385925259	0.0
72-73	6.693630096167383E-5	7.809235112195281E-4	0.0	0.5644403578593147	0.0
74-75	6.693630096167383E-5	8.032356115400861E-4	0.0	0.6267357419543125	0.0
76-77	6.693630096167383E-5	8.032356115400861E-4	0.0	0.6949326765840977	0.0
78-79	6.693630096167383E-5	8.032356115400861E-4	0.0	0.775122365136183	0.0
80-81	6.693630096167383E-5	8.032356115400861E-4	0.0	0.8615482857278642	0.0
82-83	6.693630096167383E-5	8.032356115400861E-4	0.0	0.9536637919012877	0.0
84-85	6.693630096167383E-5	8.25547711860644E-4	0.0	1.0511565142519657	0.0
86-87	6.693630096167383E-5	8.478598121812019E-4	0.0	1.1577525735334313	0.0
88	6.693630096167383E-5	8.478598121812019E-4	0.0	1.2473802805211125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9030	0.0	58.5314	1
GTATCAA	16345	0.0	40.02458	1
TATAACG	475	0.0	35.627632	2
ATAACGC	525	0.0	33.12993	3
ATCAACG	19960	0.0	32.241924	3
TCAACGC	20125	0.0	31.954224	4
CAACGCA	20790	0.0	30.99995	5
GTGGTAT	3705	0.0	30.995491	1
TATCAAC	20945	0.0	30.837872	2
AACGCAG	21840	0.0	29.567875	6
TGGTATC	3835	0.0	29.173574	2
ACGCAGA	24285	0.0	26.53294	7
CGCAGAG	24695	0.0	26.03533	8
GCAGAGT	27055	0.0	23.468958	9
TAACGCA	715	0.0	23.011244	4
GTACCTG	3525	0.0	22.430918	1
TACCTGG	3540	0.0	21.512447	2
CAGAGTA	26600	0.0	21.476294	10-11
GAGTACT	21320	0.0	21.394117	12-13
AGAGTAC	25425	0.0	21.258024	10-11
>>END_MODULE