FastQCFastQC Report
Wed 25 May 2016
SRR2049478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4168858
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51390.1232711692266803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43000.042.0042081
GTATCAA71550.033.9868551
ATCAACG73450.031.4204313
TCAACGC74600.030.807474
CAACGCA81750.027.9990185
TATCAAC86150.027.1158712
AACGCAG86350.026.5631716
TCTGTCG13650.025.4785468
GTACCTG30800.023.9761221
ACGCAGA95600.023.6979947
GTCTCGC13150.023.6074031
CGCAGAG94500.023.523668
CTGTCGC14850.022.7869839
TACCTGG31650.021.979132
GTACATG110200.021.853371
TACATGG109300.021.3296622
TATAACG4200.021.263112
TCGCTCT15550.020.8547344
CGCTCTG14300.020.7064725
ACATGGG114050.019.6582833
Produced by FastQC (version 0.11.5)