##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049478_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4168858
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.526209096112172	31.0	31.0	34.0	27.0	34.0
2	30.717304835041155	31.0	31.0	34.0	27.0	34.0
3	30.790467077554574	31.0	31.0	34.0	27.0	34.0
4	34.49976108564984	37.0	35.0	37.0	32.0	37.0
5	34.530334206634045	37.0	35.0	37.0	32.0	37.0
6	34.66976063948448	37.0	35.0	37.0	32.0	37.0
7	34.61005100197704	37.0	35.0	37.0	32.0	37.0
8	34.54316002128161	37.0	35.0	37.0	32.0	37.0
9	36.14231379432928	39.0	37.0	39.0	32.0	39.0
10-11	36.074690358846475	39.0	37.0	39.0	31.5	39.0
12-13	35.995390584183966	39.0	37.0	39.0	31.0	39.0
14-15	37.206456420439366	40.0	38.0	41.0	31.0	41.0
16-17	37.181425104908826	40.0	38.0	41.0	31.0	41.0
18-19	37.18454946174708	40.0	38.0	41.0	31.0	41.0
20-21	37.23496962477494	40.0	38.0	41.0	31.0	41.0
22-23	37.19106023759984	40.0	38.0	41.0	31.0	41.0
24-25	37.18703899245309	40.0	38.0	41.0	31.0	41.0
26-27	37.1850815019365	40.0	38.0	41.0	31.0	41.0
28-29	37.166536015378796	40.0	38.0	41.0	31.0	41.0
30-31	37.11233256205897	40.0	37.0	41.0	31.0	41.0
32-33	37.0710392390434	40.0	37.0	41.0	31.0	41.0
34-35	37.01479901690104	40.0	37.0	41.0	30.5	41.0
36-37	36.902216506295005	40.0	37.0	41.0	30.0	41.0
38-39	36.789814620694685	40.0	37.0	41.0	30.0	41.0
40-41	36.67038778965366	40.0	37.0	41.0	30.0	41.0
42-43	36.57391040903768	40.0	37.0	41.0	30.0	41.0
44-45	36.414031612494355	40.0	36.0	41.0	30.0	41.0
46-47	36.29254618411085	40.0	36.0	41.0	30.0	41.0
48-49	36.19363612289025	40.0	36.0	41.0	29.0	41.0
50-51	36.08560341944964	40.0	36.0	41.0	29.0	41.0
52-53	35.9710358328348	39.5	35.0	41.0	29.0	41.0
54-55	35.827234700726194	39.0	35.0	41.0	28.0	41.0
56-57	35.669356452054735	39.0	35.0	41.0	28.0	41.0
58-59	35.494129567377925	39.0	35.0	41.0	28.0	41.0
60-61	35.298890607451725	39.0	35.0	40.5	28.0	41.0
62-63	35.037601784469516	38.0	34.5	40.0	27.5	41.0
64-65	34.79784883534052	38.0	34.0	40.0	27.0	41.0
66-67	34.57371910964585	37.5	34.0	40.0	26.5	41.0
68-69	34.28742871548995	37.0	34.0	40.0	26.0	41.0
70-71	33.96927168063772	36.5	34.0	39.5	26.0	41.0
72-73	33.6363332596121	36.0	34.0	39.0	26.0	41.0
74-75	33.346612669464875	35.5	33.0	39.0	26.0	40.5
76-77	33.026226007218284	35.0	33.0	38.0	25.5	40.0
78-79	32.6804554148882	35.0	33.0	37.0	25.0	39.0
80-81	32.354525987692554	35.0	33.0	37.0	24.5	39.0
82-83	32.05920302394565	35.0	33.0	36.0	24.0	39.0
84-85	31.773065069618585	35.0	33.0	36.0	24.0	37.0
86-87	31.50550522469223	35.0	32.0	36.0	23.0	37.0
88-89	31.27762051381937	35.0	32.0	35.0	22.5	37.0
90-91	31.07096979556512	35.0	32.0	35.0	20.5	36.0
92-93	30.866198249016875	35.0	32.0	35.0	20.0	36.0
94-95	30.659874478814103	35.0	32.0	35.0	18.5	36.0
96-97	30.468629418416263	35.0	32.0	35.0	13.5	36.0
98-99	30.291781466291248	35.0	32.0	35.0	2.0	35.0
100	30.156995992667536	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06993251668498957
1101	2	0.04010157172758255
1101	3	0.02115022497254415
1101	4	0.15942223597847516
1101	5	0.1764361993363437
1101	6	0.21299226147217354
1101	7	0.3441217759912263
1101	8	0.32341568014247457
1101	9	0.42686048702581303
1101	10-11	0.4495223110269464
1101	12-13	0.406722194757009
1101	14-15	0.5234218886469293
1101	16-17	0.42361911238726435
1101	18-19	0.4872392228188289
1101	20-21	0.46242368598469596
1101	22-23	0.4475299972835316
1101	24-25	0.4449407346600296
1101	26-27	0.3822683656884607
1101	28-29	0.33803096291740786
1101	30-31	0.3857007031516133
1101	32-33	0.4764232182529966
1101	34-35	0.4278616764055414
1101	36-37	0.37221303872959055
1101	38-39	0.4800575014504389
1101	40-41	0.5128496914102598
1101	42-43	0.41829882882875324
1101	44-45	0.40465291600543907
1101	46-47	0.37892834236125594
1101	48-49	0.36875683225873956
1101	50-51	0.3050179236017101
1101	52-53	0.2789294908940434
1101	54-55	0.2669058736938439
1101	56-57	0.21600455474373348
1101	58-59	0.20181325557039997
1101	60-61	0.24949485601518973
1101	62-63	0.20112358034042188
1101	64-65	0.17622310785225181
1101	66-67	0.19241503680161287
1101	68-69	0.18687333955578822
1101	70-71	0.21693960586741667
1101	72-73	0.19116299539511772
1101	74-75	0.1532512137750146
1101	76-77	0.14184293408671067
1101	78-79	0.11002536224982151
1101	80-81	0.13687132483735098
1101	82-83	0.14204882919320028
1101	84-85	0.10505465488860821
1101	86-87	0.06339539681435369
1101	88-89	0.0925961316508932
1101	90-91	0.10664196302420592
1101	92-93	0.1218800167139733
1101	94-95	0.12803827587766747
1101	96-97	0.04848651046932417
1101	98-99	0.09914232581263605
1101	100	0.15245486786589524
1102	1	-0.05929226624647299
1102	2	-0.03202235387940533
1102	3	-0.008990672861063587
1102	4	0.05094848182124423
1102	5	0.027469038920948208
1102	6	0.072950366158274
1102	7	0.19986093660091342
1102	8	0.20575113130571054
1102	9	0.24793076866887986
1102	10-11	0.22897084587329886
1102	12-13	0.24715042133743026
1102	14-15	0.3540125326636172
1102	16-17	0.31887776988043726
1102	18-19	0.3172086184818994
1102	20-21	0.3070710896133093
1102	22-23	0.26675744308235494
1102	24-25	0.24067638438511096
1102	26-27	0.20484877036275861
1102	28-29	0.23095431136012223
1102	30-31	0.16030528889809403
1102	32-33	0.14826497427308283
1102	34-35	0.2158006054179893
1102	36-37	0.2467524680955151
1102	38-39	0.27677442828250776
1102	40-41	0.27081886947770073
1102	42-43	0.24947530948959695
1102	44-45	0.20536172679985754
1102	46-47	0.16603795010551892
1102	48-49	0.0940071342192752
1102	50-51	0.15755380969994803
1102	52-53	0.09095330387083322
1102	54-55	0.1038002679386949
1102	56-57	0.08810573065710514
1102	58-59	0.12686172243557792
1102	60-61	0.08578011643861316
1102	62-63	0.07107072836404171
1102	64-65	0.09806081598778604
1102	66-67	0.08536441983994081
1102	68-69	0.06394062914237963
1102	70-71	0.07269149256020313
1102	72-73	0.05058328474373752
1102	74-75	0.13892478933609453
1102	76-77	-1.309670174691746E-4
1102	78-79	0.05364831910193146
1102	80-81	0.07457298374780663
1102	82-83	0.09403392925050014
1102	84-85	0.03954109948425
1102	86-87	-0.02474530905051253
1102	88-89	-0.026960730410362288
1102	90-91	0.018724997425632495
1102	92-93	-0.010815434081163033
1102	94-95	0.025871446189004388
1102	96-97	0.017047057095656015
1102	98-99	0.022742640138154968
1102	100	-0.015378230224190048
1103	1	0.08097641305764824
1103	2	0.11482061345590822
1103	3	0.07574422790943558
1103	4	0.16307896092419583
1103	5	0.19037395807058033
1103	6	0.2668388186593731
1103	7	0.3014860786387885
1103	8	0.20294503068908654
1103	9	0.3410665157774915
1103	10-11	0.4201725053332055
1103	12-13	0.36779118266889554
1103	14-15	0.3905026868229058
1103	16-17	0.3687710878935917
1103	18-19	0.25729541304831827
1103	20-21	0.2952650627165596
1103	22-23	0.3661998835323743
1103	24-25	0.34345952168287397
1103	26-27	0.3678147414357511
1103	28-29	0.3523083337116546
1103	30-31	0.38015491729058226
1103	32-33	0.3837250414005382
1103	34-35	0.34091828183517237
1103	36-37	0.3042038108565208
1103	38-39	0.3048197160145847
1103	40-41	0.36474108907312086
1103	42-43	0.30301704129666973
1103	44-45	0.31337931449898093
1103	46-47	0.23456909421263816
1103	48-49	0.29647124238243805
1103	50-51	0.34046854969332685
1103	52-53	0.25694458628321115
1103	54-55	0.13867943088759205
1103	56-57	0.14710290303366946
1103	58-59	0.23022199318243253
1103	60-61	0.19153361410796776
1103	62-63	0.1445618222399503
1103	64-65	0.22103112551806703
1103	66-67	0.1575324113460539
1103	68-69	0.17161075894868816
1103	70-71	0.14749611369509807
1103	72-73	0.17125493216273213
1103	74-75	0.15721922484093653
1103	76-77	0.10736609054249868
1103	78-79	0.13007880252640547
1103	80-81	0.21809923438014778
1103	82-83	0.12352001695343517
1103	84-85	0.05193612279922277
1103	86-87	0.11585523681553767
1103	88-89	0.06273547589684014
1103	90-91	0.014872201620381276
1103	92-93	-0.05050863227832991
1103	94-95	0.03307514946903112
1103	96-97	0.1483329690571722
1103	98-99	0.08804146409423197
1103	100	0.03171928253485845
1104	1	-0.055219486581499666
1104	2	-0.06181178278288968
1104	3	-0.05769202402758822
1104	4	0.09928858123252837
1104	5	0.09606264260753505
1104	6	0.14816367017387932
1104	7	0.16113561471036064
1104	8	0.14564512525302575
1104	9	0.24114200662764063
1104	10-11	0.23831607977402314
1104	12-13	0.23861483172298392
1104	14-15	0.26712250351747713
1104	16-17	0.2804705666575984
1104	18-19	0.2826776457598328
1104	20-21	0.254665901470581
1104	22-23	0.23209362822494484
1104	24-25	0.24923728189094874
1104	26-27	0.21589771263172253
1104	28-29	0.20653432019720697
1104	30-31	0.15531784344472044
1104	32-33	0.1575543864342066
1104	34-35	0.21379933844081478
1104	36-37	0.22954310194894134
1104	38-39	0.21431087345084165
1104	40-41	0.2320750421459863
1104	42-43	0.22981861610345788
1104	44-45	0.24293260347243262
1104	46-47	0.1735080816571326
1104	48-49	0.1219597627332476
1104	50-51	0.18617300892439204
1104	52-53	0.2136255401061007
1104	54-55	0.2313018134831566
1104	56-57	0.2172190681963002
1104	58-59	0.25861160639133374
1104	60-61	0.303852302596745
1104	62-63	0.2506903153559179
1104	64-65	0.2503191572772039
1104	66-67	0.22231880294182815
1104	68-69	0.1682480267042834
1104	70-71	0.19605524645099592
1104	72-73	0.13802500648664306
1104	74-75	0.10646528765416718
1104	76-77	0.12048405555805886
1104	78-79	0.09996667477257404
1104	80-81	0.13396395510272185
1104	82-83	0.048784863226913444
1104	84-85	0.02959566735179564
1104	86-87	0.055216535589629245
1104	88-89	-0.007916243663007805
1104	90-91	0.013578777230708994
1104	92-93	-0.03378407389708826
1104	94-95	-0.019111768973274934
1104	96-97	0.09260666135853057
1104	98-99	0.07983381412833523
1104	100	0.03757404150821131
1105	1	-0.16884119124716435
1105	2	-0.1421086112167771
1105	3	-0.12021287105556056
1105	4	-0.07446274610411052
1105	5	-0.10398236401839966
1105	6	-0.009553050660976226
1105	7	-0.008474248183809152
1105	8	-0.010699444926302704
1105	9	-0.013394710341366078
1105	10-11	0.018380099575459496
1105	12-13	-0.0026261976237762497
1105	14-15	0.025273404523325382
1105	16-17	-0.07001838766946378
1105	18-19	-0.014592727970352826
1105	20-21	-0.03378212611364262
1105	22-23	-0.04046027695866883
1105	24-25	-0.06975707357422323
1105	26-27	-0.006771355478889518
1105	28-29	0.008806832916228302
1105	30-31	0.014746594378138411
1105	32-33	-0.03577280477082212
1105	34-35	-0.015554181930603761
1105	36-37	0.02517145149016642
1105	38-39	0.05205428270301837
1105	40-41	0.08173263507089956
1105	42-43	0.04393441742904258
1105	44-45	-0.017263014668955634
1105	46-47	-0.08543613434765263
1105	48-49	-0.0773126275090803
1105	50-51	-0.09718445212566706
1105	52-53	-0.022000755668116767
1105	54-55	-0.10197547044480615
1105	56-57	-0.11683147853474196
1105	58-59	-0.10991304485480669
1105	60-61	-0.13663248984429543
1105	62-63	-0.11045832033225622
1105	64-65	-0.12656609872838942
1105	66-67	-0.15099414117802468
1105	68-69	-0.16825164509931767
1105	70-71	-0.14696088770410398
1105	72-73	-0.20338378586299655
1105	74-75	-0.23038655083933435
1105	76-77	-0.18636245538360896
1105	78-79	-0.17713435605276828
1105	80-81	-0.19261531494775141
1105	82-83	-0.2172494134690055
1105	84-85	-0.20899803679694529
1105	86-87	-0.1981065222245988
1105	88-89	-0.15626818006926158
1105	90-91	-0.1838206851488131
1105	92-93	-0.23497386302344836
1105	94-95	-0.27191895766438634
1105	96-97	-0.23942531524498634
1105	98-99	-0.27109772646129215
1105	100	-0.3392853445262709
1106	1	-0.03480893106280192
1106	2	-0.01915577670181179
1106	3	0.018410428979134252
1106	4	0.09722951883713193
1106	5	0.0627194184805333
1106	6	0.1466906584924459
1106	7	0.1858839163562962
1106	8	0.10675324549703191
1106	9	0.12589761734151494
1106	10-11	0.1300135713778232
1106	12-13	0.18038914713224585
1106	14-15	0.23501657889611494
1106	16-17	0.27359315185987754
1106	18-19	0.25335621969898625
1106	20-21	0.20610727982035115
1106	22-23	0.25358447877849954
1106	24-25	0.25403029732117943
1106	26-27	0.23710724959643414
1106	28-29	0.2187221357616096
1106	30-31	0.2537045807713625
1106	32-33	0.22254606992306236
1106	34-35	0.2403691282379512
1106	36-37	0.197657235286961
1106	38-39	0.22581335795091917
1106	40-41	0.2644185024859951
1106	42-43	0.24737745881330397
1106	44-45	0.20603385604861302
1106	46-47	0.15868862408510864
1106	48-49	0.15448627081794797
1106	50-51	0.1665170148889672
1106	52-53	0.17723179847433812
1106	54-55	0.15660338573409405
1106	56-57	0.14784064691849608
1106	58-59	0.1517593410746798
1106	60-61	0.12955082159999876
1106	62-63	0.20109854110599912
1106	64-65	0.13896758250541552
1106	66-67	0.2471020986318493
1106	68-69	0.20633578827384724
1106	70-71	0.16809960261542045
1106	72-73	0.1329290903159901
1106	74-75	0.12994308076768135
1106	76-77	0.08704662361184035
1106	78-79	0.10947421623948372
1106	80-81	0.1526144707274355
1106	82-83	0.09600225627104919
1106	84-85	0.04979629713158218
1106	86-87	0.06226298767256111
1106	88-89	0.06359588969026575
1106	90-91	0.054711547150944284
1106	92-93	-0.02590806392081646
1106	94-95	0.1057829760051554
1106	96-97	0.18228788218296899
1106	98-99	0.19455649037420386
1106	100	0.15537768328765011
1107	1	-0.06295671478527254
1107	2	-0.10765877296417159
1107	3	-0.03199135032923195
1107	4	-0.06337031443811725
1107	5	-0.026530081003578232
1107	6	-0.03598551285139706
1107	7	-0.018468692817663168
1107	8	-0.0561040340197394
1107	9	0.044761600013075054
1107	10-11	0.02720222657874416
1107	12-13	-0.007772883356516047
1107	14-15	0.05450185040462685
1107	16-17	-0.031739733913177304
1107	18-19	0.02160113425254906
1107	20-21	0.013884920938501466
1107	22-23	0.09485585746916314
1107	24-25	0.09663752388234315
1107	26-27	0.1411169285146343
1107	28-29	0.06655932631296935
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2216	46-47	-0.13070313723547855
2216	48-49	-0.2121201225608047
2216	50-51	-0.19338556779690919
2216	52-53	-0.24039687816997457
2216	54-55	-0.2108200572147183
2216	56-57	-0.17050119362890825
2216	58-59	-0.12718256520630433
2216	60-61	-0.13402146626830813
2216	62-63	-0.1939908310192493
2216	64-65	-0.21010687950649043
2216	66-67	-0.1562073668995012
2216	68-69	-0.07936166152204294
2216	70-71	-0.006104480217295816
2216	72-73	-0.09159758761428805
2216	74-75	-0.15938977233945906
2216	76-77	-0.09574972792913172
2216	78-79	-0.12207755490963024
2216	80-81	-0.11451614624771622
2216	82-83	-0.061431116719440126
2216	84-85	-0.01491456784610179
2216	86-87	-0.10786515781777695
2216	88-89	-0.033096954904404896
2216	90-91	-0.015740576012372998
2216	92-93	-0.04911086463359737
2216	94-95	0.06480540067842
2216	96-97	-0.029252947369283078
2216	98-99	-0.06638186881329133
2216	100	-0.07648385475924968
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77487.0
3	13399.0
4	8680.0
5	6879.0
6	5777.0
7	5721.0
8	5972.0
9	6919.0
10	7812.0
11	8734.0
12	9744.0
13	10736.0
14	12126.0
15	12917.0
16	12760.0
17	13594.0
18	12727.0
19	11906.0
20	12213.0
21	13074.0
22	15027.0
23	18058.0
24	22042.0
25	27032.0
26	32506.0
27	37366.0
28	44747.0
29	55898.0
30	67753.0
31	79291.0
32	102028.0
33	135932.0
34	188564.0
35	277223.0
36	463080.0
37	838024.0
38	1097268.0
39	397002.0
40	840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92507285207314	20.50956001164769	13.81698771831316	22.748379417966007
2	16.056151849417276	21.007739291900375	39.78178363569191	23.154325222990447
3	17.976579220806016	26.095256465713206	30.58155611839127	25.34660819508951
4	12.610578017611877	18.138501313561896	39.206373995816655	30.04454667300957
5	12.670004238935887	37.412459496811685	35.10949624793001	14.80804001632242
6	29.137541831092346	38.55383766537525	18.705377169519377	13.603243334013026
7	25.909213160087496	32.721812982497475	23.310933446448402	18.058040410966626
8	24.011363407371725	35.733703605257375	21.75720192646315	18.49773106090775
9	24.12958847428612	17.87715353864519	22.584399907112605	35.40885807995609
10-11	22.85168643427581	27.38340653174361	29.54845933080012	20.216447703180464
12-13	23.641406732722295	25.20868147566325	29.244543489367068	21.90536830224738
14-15	21.391922112355	27.656624763162274	26.54999465031021	24.401458474172514
16-17	20.735520051742718	30.677436643479044	26.88472994849719	21.70231335628105
18-19	20.548757390800155	29.468239020642063	29.061044824706666	20.921958763851116
20-21	21.912229499398983	29.060401340143528	28.282841247258062	20.744527913199427
22-23	21.57373247677845	29.156992519299113	28.226139222316103	21.043135781606335
24-25	21.276336691728982	29.007280222531946	28.40665076775753	21.309732317981542
26-27	21.115029117978278	28.802000266513488	28.550335726637282	21.53263488887095
28-29	21.148639157987365	28.91372899415566	28.57406576418469	21.363566083672282
30-31	21.555409138555863	29.46192712199795	28.002587046845484	20.980076692600708
32-33	20.919322552414	29.07250875166013	28.643502276199325	21.36466641972655
34-35	21.07075935304446	28.53119664609097	28.72657825615826	21.67146574470632
36-37	21.754286176258418	29.125367431945477	27.996353088755342	21.123993303040763
38-39	21.469397038490413	29.20218364487988	28.21896044626815	21.10945887036156
40-41	21.160061324679777	28.901300540471418	28.466675487197374	21.471962647651427
42-43	21.039498086975964	28.950054587575337	28.3640687810646	21.646378544384103
44-45	21.416498322960802	29.128911292063936	28.144592448285294	21.30999793668997
46-47	21.315700294317168	29.472178420859752	27.90461412782374	21.307507156999346
48-49	21.152518308031734	28.976928665133123	28.318813583882985	21.551739442952165
50-51	21.220035288938682	28.73931406075887	28.332771573092604	21.707879077209846
52-53	21.22958166990187	28.87128875810805	28.19743183734354	21.70169773464654
54-55	21.468767501442098	29.367997099131287	27.93987342645205	21.223361972974566
56-57	21.090029882401772	29.09622229339936	28.03637471644108	21.77737310775779
58-59	20.952118114200825	28.658571250718733	28.460551638741222	21.928758996339216
60-61	21.458154140554146	29.198946355454265	27.85114877889429	21.491750725097297
62-63	21.29400649865363	29.127765430463832	28.00031332108838	21.577914749794157
64-65	21.30702052302751	28.727151931922375	28.287270799520275	21.67855674552984
66-67	21.139354734544447	28.8873766872973	28.03104755386199	21.942221024296266
68-69	21.67274770406202	29.07930610799092	27.939257039854876	21.30868914809218
70-71	21.728727768996226	28.76590636657751	28.056040220469754	21.449325643956517
72-73	21.290818985248688	28.94612714477514	28.3805010321408	21.382552837835373
74-75	21.3029998218569	28.90741897038086	28.208774117546852	21.580807090215384
76-77	21.43054542331546	29.049053049703645	27.860528292399618	21.659873234581276
78-79	21.584425535569533	29.09230683236042	28.026841390236523	21.296426241833522
80-81	21.294504779753694	28.904563153580586	28.187765044537016	21.613167022128696
82-83	21.21262100810449	28.97502439504194	28.29107558294704	21.521279013906526
84-85	21.322019626259028	28.920459793277747	28.12330645248351	21.634214127979714
86-87	21.55693341968542	29.084175652046028	27.966860567008855	21.392030361259692
88-89	21.234472026406326	28.995103093185413	28.084119845685603	21.686305034722658
90-91	21.08379928208785	28.88165341473702	28.13419612024189	21.900351182933246
92-93	21.372890654817894	29.16196177408215	27.869312777420735	21.595834793679213
94-95	21.213805304087085	28.920166156519116	28.144394808502444	21.72163373089136
96-97	21.197300930553528	28.95350902898538	28.16852954501754	21.680660495443554
98-99	21.186796051290045	28.957682112056037	28.085043601338626	21.770478235315284
100	21.394649250643205	29.04313824268485	27.925754004665865	21.636458502006082
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4699.0
1	3357.5
2	2448.0
3	2722.5
4	2327.0
5	1887.5
6	1634.5
7	1592.5
8	1641.5
9	1604.5
10	1355.5
11	1156.0
12	1147.0
13	1217.5
14	1240.0
15	1309.5
16	1530.0
17	1849.5
18	2337.5
19	3169.5
20	4383.5
21	6249.0
22	9141.0
23	13305.0
24	18837.5
25	25838.0
26	34849.5
27	45535.0
28	58418.0
29	72369.0
30	84998.0
31	96702.0
32	107598.0
33	116655.5
34	124071.5
35	130727.5
36	136255.0
37	140704.5
38	144849.5
39	146355.0
40	146647.5
41	146134.0
42	142846.5
43	139768.0
44	136229.5
45	132413.0
46	130413.5
47	128350.0
48	126268.0
49	125768.0
50	123736.5
51	117190.0
52	108270.5
53	102224.0
54	99322.0
55	95578.5
56	88667.0
57	80669.0
58	74321.5
59	68117.0
60	60197.5
61	51453.0
62	42748.0
63	34260.0
64	27053.5
65	21152.0
66	16254.5
67	12308.0
68	9283.0
69	6920.5
70	5055.0
71	3709.0
72	2803.5
73	2003.5
74	1428.5
75	1015.0
76	648.0
77	466.0
78	358.0
79	264.0
80	162.5
81	112.0
82	100.5
83	77.0
84	62.0
85	50.0
86	35.0
87	31.0
88	26.5
89	22.5
90	17.0
91	14.5
92	14.5
93	9.5
94	8.5
95	8.0
96	9.0
97	8.5
98	6.5
99	9.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07735931518895582
2	0.012425465199342362
3	0.01628743411265147
4	0.010098688897535008
5	0.00861147105514268
6	0.007388114442852215
7	0.019933516564968154
8	0.009355079976338844
9	0.008875332285244545
10-11	0.012161603969240497
12-13	0.014476386578770494
14-15	0.009403054745448274
16-17	0.013001162428655521
18-19	0.007939824287610659
20-21	0.01195771120052542
22-23	0.012761288583108371
24-25	0.015531831499177954
26-27	0.00512130660243165
28-29	0.01114214012566511
30-31	0.009558972745053922
32-33	0.012137616584685783
34-35	0.00837159720959553
36-37	0.009570966437331277
38-39	0.0187341473372324
40-41	0.013253029966480027
42-43	0.0078438747493918
44-45	0.019141932874662555
46-47	0.01764272133999287
48-49	0.017798639339598517
50-51	0.009906789821097288
52-53	0.009391061053170916
54-55	0.010830304126453815
56-57	0.02046123902517188
58-59	0.001895003379822484
60-61	0.006704473983042838
62-63	0.014632304578376141
64-65	0.024647037629969646
66-67	0.013013156120932878
68-69	0.01373277765757433
70-71	0.024707006091356433
72-73	0.019717630103975717
74-75	0.020641144409332242
76-77	0.008899319669799259
78-79	0.010674386126848168
80-81	0.019393800412487065
82-83	0.0117658121240877
84-85	0.011106159048833037
86-87	0.01562778103739681
88-89	0.014740247808872356
90-91	0.01583167380611189
92-93	0.00779589998028237
94-95	0.014860184731645932
96-97	0.012773282275385728
98-99	0.009786852898323714
100	0.006788429828984341
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4168858.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.35199752679189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.94439371835833	56.13655392963257
2	12.487236367054882	17.32029571288912
3	3.214884624636385	6.688760116101116
4	1.1890394817324683	3.2984905278547227
5	0.6142340034827243	2.129917754520269
6	0.3703170748995683	1.54093373175382
7	0.2408057013383398	1.1690249482557753
8	0.16887904244042895	0.9369679146924489
9	0.12892305099509116	0.8046964002382226
>10	0.6018611789376088	7.265619575725834
>50	0.02978143805432063	1.4217597921464886
>100	0.009402064288041695	1.0582009635598233
>500	1.3843076271710192E-4	0.05491457337020773
>1k	6.921534605705273E-5	0.0505223419026337
>5k	3.460767302835663E-5	0.12334171735696674
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5139	0.1232711692266803	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.3987384554714985E-5	0.0
5	0.0	0.0	0.0	2.3987384554714985E-5	0.0
6	0.0	0.0	0.0	2.3987384554714985E-5	0.0
7	0.0	0.0	0.0	2.3987384554714985E-5	0.0
8	0.0	0.0	0.0	4.797476910942997E-5	0.0
9	0.0	0.0	0.0	7.196215366414495E-5	0.0
10-11	0.0	0.0	0.0	9.594953821885994E-5	0.0
12-13	0.0	0.0	0.0	9.594953821885994E-5	0.0
14-15	0.0	0.0	0.0	9.594953821885994E-5	0.0
16-17	0.0	0.0	0.0	9.594953821885994E-5	0.0
18-19	0.0	0.0	0.0	9.594953821885994E-5	0.0
20-21	1.1993692277357493E-5	0.0	0.0	1.439243073282899E-4	0.0
22-23	2.3987384554714985E-5	0.0	0.0	2.2788015326979233E-4	0.0
24-25	2.3987384554714985E-5	1.1993692277357493E-5	0.0	3.9579184515279724E-4	0.0
26-27	2.3987384554714985E-5	2.3987384554714985E-5	0.0	7.55602613473522E-4	0.0
28-29	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.001439243073282899	0.0
30-31	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.0031903221457770933	0.0
32-33	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.007472070288793718	0.0
34-35	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.013888695657179975	0.0
36-37	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.023459662094511256	0.0
38-39	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.035105537295825384	0.0
40-41	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.04870638433834877	0.0
42-43	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.06252311784186461	0.0
44-45	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.07950618610660282	0.0
46-47	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.09888799282681252	0.0
48-49	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.11883350308405803	0.0
50-51	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.1395466096470544	0.0
52-53	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.15997186759539422	0.0
54-55	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.18523058353150912	0.0
56-57	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.213307817152803	0.0
58-59	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.24335201630758352	0.0
60-61	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.27659853130041845	0.0
62-63	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.3101209012156327	0.0
64-65	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.34847672911862193	0.0
66-67	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.3935130436200993	0.0
68-69	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.4425072765731047	0.0
70-71	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.4954354405930833	0.0
72-73	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.5552120029034331	0.0
74-75	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.6156122372122054	0.0
76-77	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.6825250464275827	0.0
78-79	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.7599803111547575	0.0
80-81	2.3987384554714985E-5	2.3987384554714985E-5	0.0	0.8454353686309297	0.0
82-83	2.3987384554714985E-5	3.5981076832072476E-5	0.0	0.9370311965531088	0.0
84-85	2.3987384554714985E-5	4.797476910942997E-5	0.0	1.030605983701052	0.0
86-87	2.3987384554714985E-5	4.797476910942997E-5	0.0	1.1352989235901054	0.0
88	2.3987384554714985E-5	4.797476910942997E-5	0.0	1.2223971169082757	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4300	0.0	42.004208	1
GTATCAA	7155	0.0	33.986855	1
ATCAACG	7345	0.0	31.420431	3
TCAACGC	7460	0.0	30.80747	4
CAACGCA	8175	0.0	27.999018	5
TATCAAC	8615	0.0	27.115871	2
AACGCAG	8635	0.0	26.563171	6
TCTGTCG	1365	0.0	25.478546	8
GTACCTG	3080	0.0	23.976122	1
ACGCAGA	9560	0.0	23.697994	7
GTCTCGC	1315	0.0	23.607403	1
CGCAGAG	9450	0.0	23.52366	8
CTGTCGC	1485	0.0	22.786983	9
TACCTGG	3165	0.0	21.97913	2
GTACATG	11020	0.0	21.85337	1
TACATGG	10930	0.0	21.329662	2
TATAACG	420	0.0	21.26311	2
TCGCTCT	1555	0.0	20.854734	4
CGCTCTG	1430	0.0	20.706472	5
ACATGGG	11405	0.0	19.658283	3
>>END_MODULE