FastQCFastQC Report
Wed 25 May 2016
SRR2049478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4168858
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161670.38780404609607716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151470.36333691385026784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92680.2223150800530985No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56330.1351209371967095No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42130.10105885112901423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81550.057.7637021
GTATCAA147900.040.9183121
ATCAACG182750.032.743173
TCAACGC185000.032.421174
ATAACGC4500.032.3816453
GTGGTAT34200.031.6480271
TATCAAC190800.031.509532
CAACGCA191900.031.2554245
TATAACG5050.030.716542
TGGTATC34300.030.6975462
AACGCAG200750.029.9672456
GTACCTG33550.028.1934641
TACCTGG34050.027.3336622
ACGCAGA224800.026.6563767
CGCAGAG225750.026.4192878
TAACGCA6150.024.4582084
GCAGAGT249200.023.7634629
GTACATG101400.022.555021
TACATGG102000.021.3368632
AGAGTAC235400.021.26328510-11
Produced by FastQC (version 0.11.5)