##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049478_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4168858
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.835249365653617	31.0	31.0	34.0	30.0	34.0
2	32.10971853682711	33.0	31.0	34.0	30.0	34.0
3	32.10278762193387	34.0	31.0	34.0	30.0	34.0
4	35.80757512009284	37.0	35.0	37.0	33.0	37.0
5	35.87669836679493	37.0	35.0	37.0	35.0	37.0
6	35.95681071410924	37.0	35.0	37.0	35.0	37.0
7	35.926502173976665	37.0	35.0	37.0	35.0	37.0
8	35.929935728201826	37.0	35.0	37.0	35.0	37.0
9	37.6446700751141	39.0	37.0	39.0	35.0	39.0
10-11	37.617173456135944	39.0	37.0	39.0	35.0	39.0
12-13	37.602589126326684	39.0	37.0	39.0	35.0	39.0
14-15	38.89282148732339	40.0	38.0	41.0	35.0	41.0
16-17	38.89403524898186	40.0	38.0	41.0	35.0	41.0
18-19	38.975478776202024	40.0	38.5	41.0	35.0	41.0
20-21	38.99689687199708	40.0	39.0	41.0	35.0	41.0
22-23	38.95219398214091	40.0	38.5	41.0	35.0	41.0
24-25	38.923975942572284	40.0	38.0	41.0	35.0	41.0
26-27	38.81401681227808	40.0	38.0	41.0	35.0	41.0
28-29	38.72219058552726	40.0	38.0	41.0	35.0	41.0
30-31	38.59562223035661	40.0	38.0	41.0	35.0	41.0
32-33	38.42914450432229	40.0	38.0	41.0	34.0	41.0
34-35	38.28966805777506	40.0	38.0	41.0	34.0	41.0
36-37	38.1347016377147	40.0	38.0	41.0	34.0	41.0
38-39	38.0100790192422	40.0	38.0	41.0	33.5	41.0
40-41	37.912767117517554	40.0	38.0	41.0	33.0	41.0
42-43	37.7606699484607	40.0	37.5	41.0	33.0	41.0
44-45	37.57015254537334	40.0	37.0	41.0	33.0	41.0
46-47	37.300643485578064	40.0	37.0	41.0	32.5	41.0
48-49	37.03798030539778	40.0	37.0	41.0	31.5	41.0
50-51	36.879177103177895	40.0	37.0	41.0	31.0	41.0
52-53	36.76146369101562	40.0	36.5	41.0	31.0	41.0
54-55	36.617547539398075	40.0	36.0	41.0	31.0	41.0
56-57	36.47453571217825	39.5	36.0	41.0	31.0	41.0
58-59	36.30352964768769	39.0	35.0	41.0	30.5	41.0
60-61	36.11166343396681	39.0	35.0	41.0	30.0	41.0
62-63	35.88145134710753	39.0	35.0	41.0	30.0	41.0
64-65	35.65637292035373	38.5	35.0	40.5	29.5	41.0
66-67	35.3993657255776	38.0	35.0	40.0	29.0	41.0
68-69	35.101444568272655	37.0	35.0	40.0	29.0	41.0
70-71	34.810149086392485	37.0	34.5	39.5	29.0	41.0
72-73	34.48280416363426	36.0	34.0	39.0	28.5	41.0
74-75	34.16471741181878	36.0	34.0	39.0	28.0	41.0
76-77	33.849015365838795	35.5	34.0	38.5	28.0	40.0
78-79	33.541380876969185	35.0	34.0	37.0	28.0	39.5
80-81	33.23662283531845	35.0	34.0	37.0	28.0	39.0
82-83	32.96115027184903	35.0	34.0	36.5	27.0	39.0
84-85	32.67748169882495	35.0	34.0	36.0	27.0	37.5
86-87	32.43018975460426	35.0	33.5	36.0	26.5	37.0
88-89	32.20594345022066	35.0	33.0	35.5	26.0	37.0
90-91	32.0129624228026	35.0	33.0	35.0	26.0	36.0
92-93	31.844158879961853	35.0	33.0	35.0	26.0	36.0
94-95	31.678250974247625	35.0	33.0	35.0	25.5	36.0
96-97	31.524509709853394	35.0	33.0	35.0	25.0	36.0
98-99	31.38883202546117	35.0	33.0	35.0	25.0	35.5
100	31.277256505258755	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3499024377040101
1101	2	0.35694473800989357
1101	3	0.5056760957989042
1101	4	0.3302180553248064
1101	5	0.28309792399767275
1101	6	0.26406324339650666
1101	7	0.2515938157173565
1101	8	0.26818173175883686
1101	9	0.3410199187501135
1101	10-11	0.37515099956637954
1101	12-13	0.3265931412207763
1101	14-15	0.432450219698147
1101	16-17	0.42259908892649634
1101	18-19	0.4103015229523095
1101	20-21	0.42586702148923194
1101	22-23	0.4280258677972384
1101	24-25	0.41302843613986795
1101	26-27	0.5049826249366021
1101	28-29	0.5340350543175916
1101	30-31	0.5319195180086496
1101	32-33	0.5240567735139976
1101	34-35	0.48297120037855734
1101	36-37	0.4793716267672039
1101	38-39	0.4780704385011276
1101	40-41	0.54922865363109
1101	42-43	0.5237177770211687
1101	44-45	0.5281445863293897
1101	46-47	0.5738536653448989
1101	48-49	0.5294409508984188
1101	50-51	0.4365869530353237
1101	52-53	0.4427720062774938
1101	54-55	0.4058574369933936
1101	56-57	0.45429364818922835
1101	58-59	0.4449853433458415
1101	60-61	0.3788806009355312
1101	62-63	0.40812402446901785
1101	64-65	0.40906988088178053
1101	66-67	0.4305313661887311
1101	68-69	0.43486935715505837
1101	70-71	0.4323391821469684
1101	72-73	0.4220283463677603
1101	74-75	0.3697719623668334
1101	76-77	0.37648697396997477
1101	78-79	0.35296344963233395
1101	80-81	0.3645806595024652
1101	82-83	0.3974593221273821
1101	84-85	0.4149758264345351
1101	86-87	0.3614162686653444
1101	88-89	0.34983351113488936
1101	90-91	0.3588416155451597
1101	92-93	0.3998893568126931
1101	94-95	0.3679333902786901
1101	96-97	0.28617539710988993
1101	98-99	0.40213820543566214
1101	100	0.38611110382163716
1102	1	0.3275961476175766
1102	2	0.36393435713715405
1102	3	0.4122206340028356
1102	4	0.25487044105476997
1102	5	0.26480189352216854
1102	6	0.2197514880252598
1102	7	0.24079767079447123
1102	8	0.24564043139659475
1102	9	0.2691938475546394
1102	10-11	0.2996184348302151
1102	12-13	0.2988478047623673
1102	14-15	0.3146492658884128
1102	16-17	0.35374431059972267
1102	18-19	0.34734410471754273
1102	20-21	0.3529834622955832
1102	22-23	0.3459559112662802
1102	24-25	0.28662144680031076
1102	26-27	0.3177977265396805
1102	28-29	0.3870740429591706
1102	30-31	0.314392638016713
1102	32-33	0.37450875622609203
1102	34-35	0.36107614624116025
1102	36-37	0.38384425963129587
1102	38-39	0.3888946681712184
1102	40-41	0.4526961006600132
1102	42-43	0.3182846656050984
1102	44-45	0.27425072524044936
1102	46-47	0.23198736225397454
1102	48-49	0.2996769955688876
1102	50-51	0.3012426423050272
1102	52-53	0.3046272292309027
1102	54-55	0.30226543160615194
1102	56-57	0.35552931299922363
1102	58-59	0.3872750609852176
1102	60-61	0.2678801805379578
1102	62-63	0.2207513799446872
1102	64-65	0.266046995249134
1102	66-67	0.2652805854712028
1102	68-69	0.273470910307978
1102	70-71	0.26426717150388157
1102	72-73	0.2847089883121612
1102	74-75	0.2523936484744169
1102	76-77	0.2712552550028846
1102	78-79	0.2661185618892006
1102	80-81	0.233059561171018
1102	82-83	0.19722809181858736
1102	84-85	0.1935150433302084
1102	86-87	0.23914371289161096
1102	88-89	0.24019099583650672
1102	90-91	0.2155199436139128
1102	92-93	0.19069133418391004
1102	94-95	0.17806244382736836
1102	96-97	0.23769571848047732
1102	98-99	0.3037546334868182
1102	100	0.30894642620972235
1103	1	0.33746122329061023
1103	2	0.33707295135836546
1103	3	0.43025480443811404
1103	4	0.23643716644313173
1103	5	0.24066244601453235
1103	6	0.21566105152730586
1103	7	0.23559584355827923
1103	8	0.1797082519912223
1103	9	0.268169819110625
1103	10-11	0.2874877015207815
1103	12-13	0.23035157167874587
1103	14-15	0.35955191751622806
1103	16-17	0.351625753780759
1103	18-19	0.31984595406969873
1103	20-21	0.3318866969035952
1103	22-23	0.3596406087986992
1103	24-25	0.3274484482738558
1103	26-27	0.327530657209941
1103	28-29	0.40801112431287123
1103	30-31	0.4041740034953136
1103	32-33	0.2947346525793364
1103	34-35	0.26316703692135945
1103	36-37	0.2457757797945277
1103	38-39	0.2865305902586286
1103	40-41	0.3277487002178674
1103	42-43	0.3461889958430504
1103	44-45	0.3546012420845912
1103	46-47	0.2897043084206672
1103	48-49	0.2842967408031569
1103	50-51	0.24582914580502546
1103	52-53	0.23712507059418897
1103	54-55	0.2056803143788386
1103	56-57	0.2387566422296672
1103	58-59	0.21965101034050605
1103	60-61	0.2128834979325518
1103	62-63	0.26389822740300417
1103	64-65	0.18498995385669303
1103	66-67	0.16885362704327633
1103	68-69	0.1910336971905764
1103	70-71	0.21684520653862194
1103	72-73	0.2347053870536655
1103	74-75	0.24636137103006917
1103	76-77	0.20655508731134375
1103	78-79	0.26822788253932117
1103	80-81	0.19897670002622903
1103	82-83	0.2537607323545217
1103	84-85	0.28347672593986317
1103	86-87	0.2396149269732959
1103	88-89	0.26584665790457507
1103	90-91	0.2739383725670166
1103	92-93	0.30999882488631414
1103	94-95	0.2889671816430379
1103	96-97	0.25210799733142863
1103	98-99	0.29116399828413364
1103	100	0.3003235728775415
1104	1	0.2960662632320208
1104	2	0.2380941087660915
1104	3	0.36810185755761893
1104	4	0.2684295013437463
1104	5	0.22906454095694784
1104	6	0.20902530119621332
1104	7	0.20480185288534614
1104	8	0.16432094263210928
1104	9	0.30118826987862235
1104	10-11	0.2690937859990825
1104	12-13	0.22011967470928795
1104	14-15	0.3629558389378147
1104	16-17	0.28532779274011943
1104	18-19	0.32879719638120264
1104	20-21	0.30235388025534604
1104	22-23	0.2740031743573468
1104	24-25	0.3617411824187755
1104	26-27	0.3888829011574586
1104	28-29	0.3630824534531101
1104	30-31	0.3818760957071703
1104	32-33	0.3521574734755575
1104	34-35	0.34748000565711834
1104	36-37	0.3651164894139569
1104	38-39	0.3825663764808169
1104	40-41	0.38791420137481225
1104	42-43	0.41933892322538213
1104	44-45	0.5102165346262382
1104	46-47	0.5363298049288971
1104	48-49	0.5248121380430177
1104	50-51	0.5552858521352988
1104	52-53	0.5725797144769444
1104	54-55	0.509869452037492
1104	56-57	0.44193724277928226
1104	58-59	0.4803258471902083
1104	60-61	0.4509234962643376
1104	62-63	0.45441117258268093
1104	64-65	0.4868593610708061
1104	66-67	0.4707628106131949
1104	68-69	0.38115894516450766
1104	70-71	0.36398814887771636
1104	72-73	0.3981239366104816
1104	74-75	0.3848737407567313
1104	76-77	0.4262617584229673
1104	78-79	0.35189751490825927
1104	80-81	0.3658269112090622
1104	82-83	0.3843903466936922
1104	84-85	0.35990090762760474
1104	86-87	0.36051291896014703
1104	88-89	0.3514036022816569
1104	90-91	0.37389706112643495
1104	92-93	0.39329176375448327
1104	94-95	0.31185518688460334
1104	96-97	0.318432598205991
1104	98-99	0.3647658790754278
1104	100	0.3827781109345345
1105	1	0.18340714312132178
1105	2	0.1836961952076308
1105	3	0.27317943237961373
1105	4	0.16862687299689583
1105	5	0.21203487061979587
1105	6	0.13154406490657067
1105	7	0.1275885772551959
1105	8	0.1490462352002382
1105	9	0.17531187678529392
1105	10-11	0.16808186512694334
1105	12-13	0.15135762129477115
1105	14-15	0.1287210864032886
1105	16-17	0.21228848721732163
1105	18-19	0.22901488407489978
1105	20-21	0.21724283620768858
1105	22-23	0.21355977439777263
1105	24-25	0.1758902809432854
1105	26-27	0.18288024202521314
1105	28-29	0.23769211503378784
1105	30-31	0.20539149576721627
1105	32-33	0.15849125504583128
1105	34-35	0.1895149278226711
1105	36-37	0.2218001870789763
1105	38-39	0.2045430475450729
1105	40-41	0.1652322972204061
1105	42-43	0.12049087702428096
1105	44-45	0.14406031155653665
1105	46-47	0.1528723757671031
1105	48-49	0.07845044276072599
1105	50-51	0.02269018983685811
1105	52-53	-0.005003933126189963
1105	54-55	0.006105456989963898
1105	56-57	-0.07921171585478959
1105	58-59	-0.01606809518917629
1105	60-61	-0.02932911443922137
1105	62-63	-0.10139243948268017
1105	64-65	-0.09674770232575014
1105	66-67	-0.04521996360071512
1105	68-69	0.016682858648152887
1105	70-71	0.022687572424580082
1105	72-73	0.03525756650171985
1105	74-75	-0.0755844371434975
1105	76-77	-0.056723214619758267
1105	78-79	-0.08303181666815362
1105	80-81	-0.044363313947016536
1105	82-83	-0.11415852074510013
1105	84-85	-0.06367144066869912
1105	86-87	-0.10325380529677375
1105	88-89	-0.06399160565512574
1105	90-91	-0.02614910113920388
1105	92-93	-0.07991194602840324
1105	94-95	-0.10136984577013308
1105	96-97	-0.05927211980657532
1105	98-99	-0.02390950083321286
1105	100	-0.11949253640258917
1106	1	0.1479960993646081
1106	2	0.1957167708525347
1106	3	0.2778997969387902
1106	4	0.11314152373315522
1106	5	0.14662008844781838
1106	6	0.1700454033932033
1106	7	0.13370707969080087
1106	8	0.1057380619547601
1106	9	0.16411555903552966
1106	10-11	0.1335414732677478
1106	12-13	0.1614134737364239
1106	14-15	0.24263855891994268
1106	16-17	0.24073576203844027
1106	18-19	0.23031828024780765
1106	20-21	0.1840283841752779
1106	22-23	0.2400468931036528
1106	24-25	0.2236623767561241
1106	26-27	0.19882111721426554
1106	28-29	0.21917353731227962
1106	30-31	0.19224954777934045
1106	32-33	0.1940093716436877
1106	34-35	0.21834215899359322
1106	36-37	0.21528027566627372
1106	38-39	0.1988992051037215
1106	40-41	0.18829939012832142
1106	42-43	0.250903047583094
1106	44-45	0.2525465015196886
1106	46-47	0.272397661475253
1106	48-49	0.2727098820816565
1106	50-51	0.3042208453241102
1106	52-53	0.27169866332931036
1106	54-55	0.34971614653348126
1106	56-57	0.33890474752825384
1106	58-59	0.29071709806790835
1106	60-61	0.2677760089150354
1106	62-63	0.34260226096988333
1106	64-65	0.32499155287658965
1106	66-67	0.29557638481959003
1106	68-69	0.27993555852028607
1106	70-71	0.33552704978529135
1106	72-73	0.32987309406306053
1106	74-75	0.33279239389722903
1106	76-77	0.2842216783380991
1106	78-79	0.319118728659916
1106	80-81	0.2832814575415199
1106	82-83	0.2803118698337954
1106	84-85	0.2899482886643554
1106	86-87	0.2776225716992897
1106	88-89	0.2647888356991288
1106	90-91	0.2366029824551532
1106	92-93	0.2265249306497168
1106	94-95	0.21458583185861002
1106	96-97	0.2233364953148218
1106	98-99	0.30431201775298433
1106	100	0.29860039379084213
1107	1	0.17149028108091358
1107	2	0.15217701315005883
1107	3	0.2629987726748695
1107	4	0.143827076760509
1107	5	0.18817913565688826
1107	6	0.1268370452903156
1107	7	0.11972287071089482
1107	8	0.12805116283006868
1107	9	0.11488649016590813
1107	10-11	0.12297913597812027
1107	12-13	0.1675383020577641
1107	14-15	0.1298101487497334
1107	16-17	0.18501484833216608
1107	18-19	0.1858156700488962
1107	20-21	0.1825245303737546
1107	22-23	0.1963767962803118
1107	24-25	0.19049166838094322
1107	26-27	0.2068555076098164
1107	28-29	0.2286045037008435
1107	30-31	0.2022637945456296
1107	32-33	0.1695560101584732
1107	34-35	0.20921218543723086
1107	36-37	0.1983244030603828
1107	38-39	0.18176584580506727
1107	40-41	0.1930308881228484
1107	42-43	0.1221033120194761
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2216	80-81	-0.9665412052847344
2216	82-83	-0.9921871299866503
2216	84-85	-1.052203005688753
2216	86-87	-0.9470422237653011
2216	88-89	-0.9539001887871592
2216	90-91	-0.8981677392877216
2216	92-93	-0.926238347587109
2216	94-95	-0.9220755530199334
2216	96-97	-0.9519566369255443
2216	98-99	-1.1057146292006088
2216	100	-1.1263506472884508
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	272.0
3	33.0
4	46.0
5	132.0
6	410.0
7	992.0
8	2149.0
9	3502.0
10	4575.0
11	6103.0
12	8997.0
13	13184.0
14	14126.0
15	16516.0
16	20758.0
17	29112.0
18	28887.0
19	15408.0
20	11117.0
21	10282.0
22	10573.0
23	11578.0
24	13173.0
25	16623.0
26	20585.0
27	26796.0
28	34390.0
29	44923.0
30	58624.0
31	75653.0
32	98476.0
33	128482.0
34	178956.0
35	266951.0
36	431712.0
37	840210.0
38	1217417.0
39	505519.0
40	1616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58752929010418	20.43831468637104	14.42315936146192	22.550996662062865
2	16.025036113007445	21.316221372855587	39.46824286171417	23.190499652422798
3	17.70700753059951	26.19813387743118	31.236204255457974	24.858654336511343
4	12.594792146914093	18.325186417959067	38.65166431670256	30.428357118424277
5	12.03404224693224	37.11780440701957	35.43423656842636	15.413916777621841
6	28.75254026524303	38.7441782589756	18.28683936312504	14.216442112656324
7	25.168355458497267	33.09877669136248	23.459758044049472	18.27310980609078
8	24.256810858033543	35.064111082699384	21.662959016593994	19.016119042673076
9	24.14339850385885	16.822544687298056	22.92311227679139	36.110944532051704
10-11	22.80724121569984	26.991396684655605	29.567641785831995	20.63372031381256
12-13	24.14502964600857	24.787339842230175	29.691140835211943	21.37648967654931
14-15	21.53665344322114	26.799629538832935	27.852471828016213	23.81124518992971
16-17	20.053945229125098	29.83636046130619	28.27539340510039	21.834300904468325
18-19	19.738619065461094	27.751736806578688	31.84748676975805	20.662157358202172
20-21	21.067268302254476	27.262897896738146	31.515633298135842	20.15420050287153
22-23	20.727990735112588	27.347873206523225	31.51674871151764	20.407387346846548
24-25	20.44144943291424	27.245195206936767	31.681386125408924	20.631969234740065
26-27	20.360468022657525	27.059233008176342	31.761396046591177	20.81890292257496
28-29	20.41754600420547	27.125270277855467	31.77677196009075	20.68041175784831
30-31	20.788378975729085	27.654443974824762	31.239814356833456	20.3173626926127
32-33	20.171195564828544	27.313235423226217	31.819457510905863	20.696111501039375
34-35	20.323095677521277	26.899261140580943	31.736161318039617	21.04148186385816
36-37	21.087393717895882	27.47325526559072	30.9285420611592	20.5108089553542
38-39	20.84626053465961	27.56360374951605	31.06199587512935	20.52813984069498
40-41	20.486005999724625	27.360394621260788	31.21260066905613	20.940998709958457
42-43	20.48243187942597	27.47685337327393	30.90225188768723	21.138462859612872
44-45	20.92387171738639	27.73213911339748	30.52801270755684	20.815976461659282
46-47	20.936932848276435	28.145909503274037	29.946894329334317	20.97026331911521
48-49	20.889869599780084	27.815183438725903	30.05875949720523	21.23618746428878
50-51	20.979270582015506	27.68544766936173	29.853319542186373	21.481962206436393
52-53	21.019929198835747	27.921831350456166	29.62942129475266	21.42881815595542
54-55	21.201669617914547	28.39669760879358	29.388815833976594	21.012816939315275
56-57	20.801584030926453	28.084621735736743	29.479955901592238	21.633838331744567
58-59	20.658331370365698	27.62544322689811	29.961778501450514	21.754446901285675
60-61	21.1927942856293	28.13627856837532	29.36196195696759	21.308965189027788
62-63	20.977087730021026	28.004443902862608	29.59376404761208	21.424704319504286
64-65	20.995677953050933	27.65848584912223	29.866260736153645	21.479575461673196
66-67	20.891644666237134	27.817078442105732	29.56801359029259	21.723263301364547
68-69	21.48942228303291	28.00775416193116	29.345782466085435	21.1570410889505
70-71	21.48509256012078	27.71420854344283	29.497622610316782	21.303076286119605
72-73	21.12240330565349	27.908926137565732	29.727169886813126	21.24150066996765
74-75	21.165823010261203	27.882663295639155	29.504366604039596	21.447147090060046
76-77	21.31652121516252	28.03012239802843	29.186662150641734	21.466694236167314
78-79	21.465638791246906	28.140932120978935	29.24956906663648	21.143860021137684
80-81	21.14669259547686	27.88826320348979	29.47932529602585	21.48571890500751
82-83	21.086241250402242	27.977555837248996	29.553955667945083	21.382247244403683
84-85	21.141278561288722	27.969421724306176	29.356785409473424	21.53251430493168
86-87	21.371486278540647	28.074150212229554	29.218654612139844	21.335708897089955
88-89	20.993747892096447	28.01242505698236	29.309814207042066	21.684012843879128
90-91	20.858792617927886	27.887084372244054	29.353767046601448	21.90035596322661
92-93	21.129141400899865	28.20201691162237	29.070434069451785	21.598407618025984
94-95	20.98658969251799	27.925381259631717	29.318248901284537	21.769780146565758
96-97	20.882728628021976	27.979627401900053	29.355755264960116	21.781888705117858
98-99	20.883003854342878	27.977388718682867	29.30405219338688	21.835555233587378
100	21.057355838010082	28.048944889384416	29.11554547870733	21.778153793898174
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2056.0
1	1475.5
2	2871.0
3	4336.0
4	2370.5
5	1039.5
6	2034.5
7	4807.0
8	7629.5
9	8493.0
10	6417.5
11	3682.0
12	3060.0
13	3378.5
14	3852.5
15	4309.5
16	4838.5
17	5453.5
18	6157.5
19	7107.5
20	8227.0
21	9846.5
22	12635.5
23	16819.5
24	21990.0
25	28399.0
26	36876.5
27	46837.0
28	59098.0
29	72656.5
30	84737.0
31	96044.0
32	106589.5
33	115521.0
34	122707.0
35	128735.0
36	133787.5
37	137801.5
38	142112.5
39	144170.5
40	144378.5
41	143346.0
42	140128.0
43	137544.0
44	134684.0
45	131584.5
46	128980.0
47	126291.0
48	124588.0
49	124162.0
50	121826.5
51	115870.0
52	107379.0
53	100503.0
54	96316.5
55	92195.5
56	86231.0
57	78457.5
58	72071.0
59	66885.5
60	59441.5
61	50000.5
62	41109.0
63	33313.5
64	26267.5
65	19997.0
66	15208.5
67	11346.0
68	8338.5
69	6171.0
70	4353.5
71	3118.5
72	2360.0
73	1605.0
74	1044.0
75	685.5
76	428.0
77	277.5
78	182.5
79	109.5
80	60.0
81	33.0
82	18.5
83	11.5
84	8.5
85	5.5
86	4.5
87	2.0
88	1.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11775407077909586
2	0.0
3	0.0
4	0.0
5	0.012185591353795211
6	4.797476910942997E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.1993692277357493E-5
76-77	0.0
78-79	0.0
80-81	1.7990538416036238E-4
82-83	5.637035370358021E-4
84-85	3.8379815287543977E-4
86-87	5.517098447584446E-4
88-89	5.756972293131596E-4
90-91	6.83640459809377E-4
92-93	2.1588646099243486E-4
94-95	2.9984230693393734E-4
96-97	5.517098447584446E-4
98-99	0.0012953187659546091
100	1.439243073282899E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4168858.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.19446644233813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58388438745169	52.68012764436089
2	13.216643716875486	17.49737357994107
3	3.5079220438776657	6.966150840473949
4	1.3518649274061976	3.579439102870537
5	0.6552453596501119	2.1686808485428557
6	0.38875960690103073	1.5440240851888105
7	0.2709660786143711	1.2555518498495661
8	0.18233913479756125	0.9655873391585711
9	0.13334674817047468	0.7944135162268859
>10	0.6599872364548578	7.694317152816526
>50	0.03385642564979093	1.5157733615433828
>100	0.014350952553673552	1.5957945584916946
>500	3.6233984834009196E-4	0.17240340619082722
>1k	3.261058260085059E-4	0.46190101496577385
>5k	7.246796133515865E-5	0.35739884847866255
>10k+	7.246796133515865E-5	0.7510628508999959
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16167	0.38780404609607716	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15147	0.36333691385026784	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9268	0.2223150800530985	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5633	0.1351209371967095	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4213	0.10105885112901423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.3987384554714985E-5	0.0	0.0	0.0
4	0.0	2.3987384554714985E-5	0.0	2.3987384554714985E-5	0.0
5	0.0	2.3987384554714985E-5	0.0	4.797476910942997E-5	0.0
6	0.0	2.3987384554714985E-5	0.0	4.797476910942997E-5	0.0
7	0.0	2.3987384554714985E-5	0.0	4.797476910942997E-5	0.0
8	0.0	2.3987384554714985E-5	0.0	4.797476910942997E-5	0.0
9	0.0	2.3987384554714985E-5	0.0	4.797476910942997E-5	0.0
10-11	0.0	2.3987384554714985E-5	0.0	4.797476910942997E-5	0.0
12-13	0.0	2.3987384554714985E-5	0.0	4.797476910942997E-5	0.0
14-15	0.0	2.3987384554714985E-5	0.0	5.9968461386787465E-5	0.0
16-17	0.0	2.3987384554714985E-5	0.0	8.395584594150245E-5	0.0
18-19	0.0	2.3987384554714985E-5	0.0	9.594953821885994E-5	0.0
20-21	0.0	2.3987384554714985E-5	0.0	1.439243073282899E-4	0.0
22-23	0.0	2.3987384554714985E-5	0.0	2.2788015326979233E-4	0.0
24-25	0.0	2.3987384554714985E-5	0.0	3.837981528754397E-4	0.0
26-27	0.0	2.3987384554714985E-5	0.0	7.55602613473522E-4	0.0
28-29	0.0	2.3987384554714985E-5	0.0	0.001439243073282899	0.0
30-31	0.0	2.3987384554714985E-5	0.0	0.0031183599921129476	0.0
32-33	0.0	2.3987384554714985E-5	0.0	0.007388114442852214	0.0
34-35	0.0	4.797476910942997E-5	0.0	0.013804739811238472	0.0
36-37	0.0	8.395584594150245E-5	0.0	0.02349564317134333	0.0
38-39	0.0	9.594953821885994E-5	0.0	0.03520148683404424	0.0
40-41	0.0	9.594953821885994E-5	0.0	0.048862302337954425	0.0
42-43	0.0	9.594953821885994E-5	0.0	0.06272701061057968	0.0
44-45	0.0	1.0794323049621743E-4	0.0	0.08000992118225184	0.0
46-47	0.0	1.1993692277357492E-4	0.0	0.09959562067117661	0.0
48-49	0.0	1.439243073282899E-4	0.0	0.11933723815970704	0.0
50-51	0.0	1.439243073282899E-4	0.0	0.14009831949181284	0.0
52-53	0.0	1.439243073282899E-4	0.0	0.16058354590153945	0.0
54-55	0.0	1.439243073282899E-4	0.0	0.18578229337626756	0.0
56-57	0.0	1.439243073282899E-4	0.0	0.2137875648438973	0.0
58-59	0.0	1.439243073282899E-4	0.0	0.2440596441519476	0.0
60-61	0.0	1.439243073282899E-4	0.0	0.2774021086830014	1.1993692277357493E-5
62-63	0.0	1.439243073282899E-4	0.0	0.3109004912136609	2.3987384554714985E-5
64-65	0.0	1.439243073282899E-4	0.0	0.34974806050002183	2.3987384554714985E-5
66-67	0.0	1.439243073282899E-4	0.0	0.39502424884704634	2.3987384554714985E-5
68-69	0.0	1.439243073282899E-4	0.0	0.4442943367224309	2.3987384554714985E-5
70-71	0.0	1.439243073282899E-4	0.0	0.4974503808956794	2.3987384554714985E-5
72-73	0.0	1.439243073282899E-4	0.0	0.5573468801288026	2.3987384554714985E-5
74-75	0.0	1.439243073282899E-4	0.0	0.6180589504367863	2.3987384554714985E-5
76-77	0.0	1.439243073282899E-4	0.0	0.6851996398054335	2.3987384554714985E-5
78-79	0.0	1.439243073282899E-4	0.0	0.7628468036090459	2.3987384554714985E-5
80-81	0.0	1.439243073282899E-4	0.0	0.8479780313937294	2.3987384554714985E-5
82-83	0.0	1.439243073282899E-4	0.0	0.9399336700842293	2.3987384554714985E-5
84-85	0.0	1.439243073282899E-4	0.0	1.0335084572321724	2.3987384554714985E-5
86-87	0.0	1.439243073282899E-4	0.0	1.1388130754273713	2.3987384554714985E-5
88	0.0	1.439243073282899E-4	0.0	1.2260431993605923	2.3987384554714985E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8155	0.0	57.763702	1
GTATCAA	14790	0.0	40.918312	1
ATCAACG	18275	0.0	32.74317	3
TCAACGC	18500	0.0	32.42117	4
ATAACGC	450	0.0	32.381645	3
GTGGTAT	3420	0.0	31.648027	1
TATCAAC	19080	0.0	31.50953	2
CAACGCA	19190	0.0	31.255424	5
TATAACG	505	0.0	30.71654	2
TGGTATC	3430	0.0	30.697546	2
AACGCAG	20075	0.0	29.967245	6
GTACCTG	3355	0.0	28.193464	1
TACCTGG	3405	0.0	27.333662	2
ACGCAGA	22480	0.0	26.656376	7
CGCAGAG	22575	0.0	26.419287	8
TAACGCA	615	0.0	24.458208	4
GCAGAGT	24920	0.0	23.763462	9
GTACATG	10140	0.0	22.55502	1
TACATGG	10200	0.0	21.336863	2
AGAGTAC	23540	0.0	21.263285	10-11
>>END_MODULE