FastQCFastQC Report
Wed 25 May 2016
SRR2049477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110501
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70150.044.246231
GTATCAA119450.033.8588561
TCAACGC126150.030.3252074
ATCAACG127250.030.0645453
CAACGCA137200.028.0214275
TATCAAC145900.027.0268042
AACGCAG143150.026.8578576
ACGCAGA161550.023.3336627
GTACATG198500.022.7678951
CGCAGAG164950.022.6518048
TACATGG201200.021.5840052
ACATGGG207000.020.298093
GCAGAGT191950.018.5839779
GTGGTAT42600.017.7736361
CATGGGG138100.017.6620184
AGAGTAC168500.017.33533910-11
CAGAGTA184950.016.61936610-11
GAGTACA154300.015.5440511
TGGTATC45200.015.0770922
GAGTACT79600.014.84875412-13
Produced by FastQC (version 0.11.5)