##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049477_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7110501
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.926990517264535	31.0	31.0	34.0	28.0	34.0
2	31.106266632970026	31.0	31.0	34.0	28.0	34.0
3	31.182501626819263	31.0	31.0	34.0	28.0	34.0
4	34.871594420702564	37.0	35.0	37.0	32.0	37.0
5	34.90176332159998	37.0	35.0	37.0	32.0	37.0
6	35.015022851413704	37.0	35.0	37.0	32.0	37.0
7	34.95117643609079	37.0	35.0	37.0	32.0	37.0
8	34.901639279707574	37.0	35.0	37.0	32.0	37.0
9	36.53695836622483	39.0	37.0	39.0	32.0	39.0
10-11	36.48069376546041	39.0	37.0	39.0	32.0	39.0
12-13	36.4108851120336	39.0	37.0	39.0	32.0	39.0
14-15	37.66333701380536	40.0	38.0	41.0	32.0	41.0
16-17	37.64862897846439	40.0	38.0	41.0	32.0	41.0
18-19	37.663099477800515	40.0	38.0	41.0	32.0	41.0
20-21	37.708664269929784	40.0	38.0	41.0	32.0	41.0
22-23	37.668848791386154	40.0	38.0	41.0	32.0	41.0
24-25	37.66919089104973	40.0	38.0	41.0	32.0	41.0
26-27	37.663943370516364	40.0	38.0	41.0	32.0	41.0
28-29	37.64367946787435	40.0	38.0	41.0	32.0	41.0
30-31	37.592862373551455	40.0	38.0	41.0	32.0	41.0
32-33	37.550671745914954	40.0	38.0	41.0	32.0	41.0
34-35	37.49029301873384	40.0	38.0	41.0	32.0	41.0
36-37	37.388717967974415	40.0	38.0	41.0	32.0	41.0
38-39	37.28829227363867	40.0	37.5	41.0	31.0	41.0
40-41	37.17417985033684	40.0	37.0	41.0	31.0	41.0
42-43	37.08934447797701	40.0	37.0	41.0	31.0	41.0
44-45	36.9467269605897	40.0	37.0	41.0	30.5	41.0
46-47	36.83277507449897	40.0	37.0	41.0	30.0	41.0
48-49	36.740364919433944	40.0	37.0	41.0	30.0	41.0
50-51	36.63438546735314	40.0	36.0	41.0	30.0	41.0
52-53	36.530679624403405	40.0	36.0	41.0	30.0	41.0
54-55	36.38990529640598	40.0	36.0	41.0	30.0	41.0
56-57	36.23446589769132	39.0	35.0	41.0	30.0	41.0
58-59	36.062925242539166	39.0	35.0	41.0	29.0	41.0
60-61	35.87760679592057	39.0	35.0	41.0	29.0	41.0
62-63	35.633667866722746	39.0	35.0	40.5	28.5	41.0
64-65	35.40139218038223	38.0	35.0	40.0	28.0	41.0
66-67	35.182852164706816	38.0	35.0	40.0	28.0	41.0
68-69	34.90207926276925	37.0	34.0	40.0	28.0	41.0
70-71	34.59090815119778	37.0	34.0	40.0	28.0	41.0
72-73	34.25890918234875	36.0	34.0	39.0	28.0	41.0
74-75	33.95368329179618	36.0	34.0	39.0	27.5	41.0
76-77	33.62198444244646	35.5	34.0	38.5	27.0	40.0
78-79	33.268590145757656	35.0	34.0	37.0	26.0	39.5
80-81	32.93080733692324	35.0	33.0	37.0	26.0	39.0
82-83	32.625576945984534	35.0	33.0	36.5	26.0	39.0
84-85	32.335149450087975	35.0	33.0	36.0	26.0	37.5
86-87	32.06168285469617	35.0	33.0	36.0	26.0	37.0
88-89	31.833426575708238	35.0	33.0	35.5	25.0	37.0
90-91	31.62533469863797	35.0	33.0	35.0	25.0	36.0
92-93	31.424837363780696	35.0	33.0	35.0	25.0	36.0
94-95	31.228174146941264	35.0	33.0	35.0	24.0	36.0
96-97	31.04823098963069	35.0	32.5	35.0	23.5	36.0
98-99	30.88316280385869	35.0	32.0	35.0	21.5	35.5
100	30.75732567930164	35.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1737783001364761
1101	2	0.18855219748695617
1101	3	0.1870343858000325
1101	4	0.3051534156053748
1101	5	0.2208533662297043
1101	6	0.31703951916102824
1101	7	0.3626341058577722
1101	8	0.34898004289777873
1101	9	0.41431948424420995
1101	10-11	0.44280788735613186
1101	12-13	0.45907208004240374
1101	14-15	0.573471128004627
1101	16-17	0.5521302805818138
1101	18-19	0.5969740437120947
1101	20-21	0.5465818413333139
1101	22-23	0.5503394742635024
1101	24-25	0.5634052911130141
1101	26-27	0.5074696414420856
1101	28-29	0.49928744113373824
1101	30-31	0.5081728231321492
1101	32-33	0.5116540541137979
1101	34-35	0.46820209263434265
1101	36-37	0.4771853914887032
1101	38-39	0.5027168418278833
1101	40-41	0.5560800815744926
1101	42-43	0.509670868470792
1101	44-45	0.5504359091103339
1101	46-47	0.4622475958004344
1101	48-49	0.46523705199078336
1101	50-51	0.45687939570503033
1101	52-53	0.44355244715239905
1101	54-55	0.45218189104486584
1101	56-57	0.42944687115817004
1101	58-59	0.4215892621550168
1101	60-61	0.4459254745097354
1101	62-63	0.42718048024174493
1101	64-65	0.47861161923402307
1101	66-67	0.41305878289665543
1101	68-69	0.3627794995581439
1101	70-71	0.4115440183365138
1101	72-73	0.38063271206605975
1101	74-75	0.37027083029791186
1101	76-77	0.33118887440300426
1101	78-79	0.34504170057243755
1101	80-81	0.3790644365775009
1101	82-83	0.34647468713207985
1101	84-85	0.27245030066004716
1101	86-87	0.3031021268839922
1101	88-89	0.3099569676742391
1101	90-91	0.29985602067135275
1101	92-93	0.27956324648564745
1101	94-95	0.32728462969941674
1101	96-97	0.31400225730037334
1101	98-99	0.3235351879301511
1101	100	0.3402412387710072
1102	1	0.08094111928453884
1102	2	0.16164872090907423
1102	3	0.2038540262177655
1102	4	0.21317136831747518
1102	5	0.1903765837207203
1102	6	0.28590172401118963
1102	7	0.3623326217873313
1102	8	0.3657979892855039
1102	9	0.47476772756811414
1102	10-11	0.44845074926449513
1102	12-13	0.43841188714161206
1102	14-15	0.5384982939599681
1102	16-17	0.5181313912688594
1102	18-19	0.5066614843078483
1102	20-21	0.4770264766929273
1102	22-23	0.5073224374995817
1102	24-25	0.5061569372999983
1102	26-27	0.45187404829813715
1102	28-29	0.4641437764149643
1102	30-31	0.40997932714622465
1102	32-33	0.41060007658567343
1102	34-35	0.4376227680383522
1102	36-37	0.4782321528751581
1102	38-39	0.4560986648347338
1102	40-41	0.4371026006457015
1102	42-43	0.34731890511876884
1102	44-45	0.38255607545939085
1102	46-47	0.3455350969209974
1102	48-49	0.37653362931927603
1102	50-51	0.3963695600069812
1102	52-53	0.4366230973025864
1102	54-55	0.3819982650104876
1102	56-57	0.35223384096060784
1102	58-59	0.38157055196645473
1102	60-61	0.33834031876412496
1102	62-63	0.32652604874766666
1102	64-65	0.3094439605298831
1102	66-67	0.32202457203518264
1102	68-69	0.3050695065859159
1102	70-71	0.25180993864410084
1102	72-73	0.2686830211775231
1102	74-75	0.2870931364156988
1102	76-77	0.2841546212653512
1102	78-79	0.31631770108307933
1102	80-81	0.3181228043495494
1102	82-83	0.31046442484743153
1102	84-85	0.2714779327613073
1102	86-87	0.2327505574845148
1102	88-89	0.21012476933746882
1102	90-91	0.22670263750483954
1102	92-93	0.258693467978901
1102	94-95	0.2761775821531529
1102	96-97	0.3262334416397934
1102	98-99	0.293573715499722
1102	100	0.259467203004899
1103	1	0.16407596268381042
1103	2	0.2385717728204746
1103	3	0.21365825739784938
1103	4	0.2969305438219223
1103	5	0.2763629881077492
1103	6	0.3135794075322025
1103	7	0.3781063149133317
1103	8	0.35329100487236076
1103	9	0.41548860130791354
1103	10-11	0.43911983241871866
1103	12-13	0.390260883189697
1103	14-15	0.49486425312679216
1103	16-17	0.4757768176988435
1103	18-19	0.4694645804033115
1103	20-21	0.49813953329565663
1103	22-23	0.47157396802467133
1103	24-25	0.5024632179017345
1103	26-27	0.5017739879113492
1103	28-29	0.4594598427650354
1103	30-31	0.5161761768926638
1103	32-33	0.5191409761608128
1103	34-35	0.4915782702012734
1103	36-37	0.49473756965016946
1103	38-39	0.5345429546965832
1103	40-41	0.5680610861780195
1103	42-43	0.4972979328274931
1103	44-45	0.45979434172153333
1103	46-47	0.43431263486545646
1103	48-49	0.46324585889839653
1103	50-51	0.523097597044206
1103	52-53	0.4550398376618574
1103	54-55	0.4145366072667045
1103	56-57	0.3959942693155938
1103	58-59	0.4231383140593792
1103	60-61	0.429368887719221
1103	62-63	0.4281852628334093
1103	64-65	0.4747292807287167
1103	66-67	0.44468963407769735
1103	68-69	0.40564703207299146
1103	70-71	0.41075723224814453
1103	72-73	0.4177525281671137
1103	74-75	0.3948478646563629
1103	76-77	0.3642197979442088
1103	78-79	0.42891258317337844
1103	80-81	0.376399650177909
1103	82-83	0.3135989999964721
1103	84-85	0.3549630510205475
1103	86-87	0.3891846003905286
1103	88-89	0.3914148080986024
1103	90-91	0.35036325340128727
1103	92-93	0.3114344925952288
1103	94-95	0.3628888362768663
1103	96-97	0.3566954102083848
1103	98-99	0.36433430246782095
1103	100	0.33416188800240576
1104	1	0.07632658561250594
1104	2	0.05709197166277846
1104	3	0.11167293462989036
1104	4	0.2213418236236322
1104	5	0.19511419346513748
1104	6	0.23241302476586156
1104	7	0.2519309377247083
1104	8	0.2253593610261575
1104	9	0.31888051519201355
1104	10-11	0.3782380224315105
1104	12-13	0.36794398871138867
1104	14-15	0.43036936209546894
1104	16-17	0.3743118017732101
1104	18-19	0.3820194082444388
1104	20-21	0.3675422247668152
1104	22-23	0.4023708000434567
1104	24-25	0.40770140816487555
1104	26-27	0.40947687839656766
1104	28-29	0.3198220591176053
1104	30-31	0.3179383966511651
1104	32-33	0.3066365386262291
1104	34-35	0.32466586709745116
1104	36-37	0.3592024186269853
1104	38-39	0.3403191946627331
1104	40-41	0.39904792254736776
1104	42-43	0.3782377629880074
1104	44-45	0.34835853674512407
1104	46-47	0.34420322443931894
1104	48-49	0.35007767244436394
1104	50-51	0.32781639744634106
1104	52-53	0.28323388371673275
1104	54-55	0.2777319611115914
1104	56-57	0.2076855336421488
1104	58-59	0.28636749432612163
1104	60-61	0.28162963237500094
1104	62-63	0.32497248052142425
1104	64-65	0.32571300518368673
1104	66-67	0.31509901092485393
1104	68-69	0.270753491420372
1104	70-71	0.2308098815308881
1104	72-73	0.2361284261720158
1104	74-75	0.2632202039004383
1104	76-77	0.2424660027695893
1104	78-79	0.23717857296227862
1104	80-81	0.25811280124737834
1104	82-83	0.21400068147820406
1104	84-85	0.19258151081506014
1104	86-87	0.1666212853779001
1104	88-89	0.19301724022415812
1104	90-91	0.21927218682247585
1104	92-93	0.20230475007339166
1104	94-95	0.24745522565129363
1104	96-97	0.3196023661520897
1104	98-99	0.3043389514175381
1104	100	0.2850330857502996
1105	1	0.0864806204543882
1105	2	0.11979299474355187
1105	3	0.1320177731618024
1105	4	0.19358958601979026
1105	5	0.1826303396172264
1105	6	0.20471348823996038
1105	7	0.2630278248586464
1105	8	0.2514803783613928
1105	9	0.2778668202730188
1105	10-11	0.30213896466020174
1105	12-13	0.3239446511320594
1105	14-15	0.36316587587227644
1105	16-17	0.31829721857848625
1105	18-19	0.3401738849501612
1105	20-21	0.30223230140234136
1105	22-23	0.3263965697452704
1105	24-25	0.31153934072222
1105	26-27	0.3008151936697061
1105	28-29	0.3337465184990194
1105	30-31	0.32248073256642584
1105	32-33	0.2974500421240336
1105	34-35	0.31081962005420394
1105	36-37	0.3671952075816307
1105	38-39	0.355804299879928
1105	40-41	0.3873943732446392
1105	42-43	0.31583884274119356
1105	44-45	0.31750844632104247
1105	46-47	0.2539099284055979
1105	48-49	0.2639697422309055
1105	50-51	0.24053571352138192
1105	52-53	0.248672249548207
1105	54-55	0.2346810882077932
1105	56-57	0.19277300875741332
1105	58-59	0.19449055903258738
1105	60-61	0.1633463062224294
1105	62-63	0.23257079909447498
1105	64-65	0.2093509990411988
1105	66-67	0.22240224097111394
1105	68-69	0.17462786930501295
1105	70-71	0.20153350906048217
1105	72-73	0.23165035837732972
1105	74-75	0.23490918952050066
1105	76-77	0.19745601886921094
1105	78-79	0.18653968726562198
1105	80-81	0.20218106283433457
1105	82-83	0.15435282314170706
1105	84-85	0.14702158366445417
1105	86-87	0.12354429168181724
1105	88-89	0.1043457354672519
1105	90-91	0.14460039989912232
1105	92-93	0.09532167907561728
1105	94-95	0.1337847817145743
1105	96-97	0.17378697943472332
1105	98-99	0.17142269741578176
1105	100	0.08689008606402027
1106	1	0.17507522355094096
1106	2	0.20310232423626928
1106	3	0.2169111506813799
1106	4	0.2628069831999156
1106	5	0.26572471888338356
1106	6	0.2895654554248992
1106	7	0.3571174926149041
1106	8	0.3144863166581686
1106	9	0.39381918149532424
1106	10-11	0.369594642019365
1106	12-13	0.36718973296997603
1106	14-15	0.4085352348270561
1106	16-17	0.3973059227386244
1106	18-19	0.39328003671333533
1106	20-21	0.4126444666355127
1106	22-23	0.3949663540348638
1106	24-25	0.4195850217544361
1106	26-27	0.44815843763295504
1106	28-29	0.4109204982901389
1106	30-31	0.4022363294105631
1106	32-33	0.40214892803992797
1106	34-35	0.4580015828629769
1106	36-37	0.4511714626126775
1106	38-39	0.48380499111748776
1106	40-41	0.5675611970833856
1106	42-43	0.47623186653798655
1106	44-45	0.3654846018211799
1106	46-47	0.41157575978882477
1106	48-49	0.40631838391942665
1106	50-51	0.45709069327391916
1106	52-53	0.44727724175351824
1106	54-55	0.3919802988775061
1106	56-57	0.392265652259411
1106	58-59	0.4181414073623273
1106	60-61	0.412568036192809
1106	62-63	0.4353665445592583
1106	64-65	0.34392657392918835
1106	66-67	0.44155657527943504
1106	68-69	0.4108018283224766
1106	70-71	0.3944256556841168
1106	72-73	0.4001071659601223
1106	74-75	0.3776092026923621
1106	76-77	0.34069418376589766
1106	78-79	0.3492125166301605
1106	80-81	0.3532506890387239
1106	82-83	0.3400586102473895
1106	84-85	0.34894588584667474
1106	86-87	0.33785256674049435
1106	88-89	0.351921301908817
1106	90-91	0.34029354247097743
1106	92-93	0.2735724645365245
1106	94-95	0.2644502987536477
1106	96-97	0.3923122601700797
1106	98-99	0.4873345157746236
1106	100	0.4599682550332531
1107	1	0.04753242143754832
1107	2	0.04716273214534894
1107	3	0.12055548520226722
1107	4	0.1234710153436751
1107	5	0.12521216625456333
1107	6	0.1098170343729663
1107	7	0.16779412880310218
1107	8	0.15286959643263742
1107	9	0.1642090273661836
1107	10-11	0.20639956228296086
1107	12-13	0.2322486121805838
1107	14-15	0.26881001854276576
1107	16-17	0.1945701676963978
1107	18-19	0.18723062841492322
1107	20-21	0.22175620243341854
1107	22-23	0.20817916827257932
1107	24-25	0.2421410198026379
1107	26-27	0.2606994729591463
1107	28-29	0.27360684103619803
1107	30-31	0.24327263283422695
1107	32-33	0.21750940482150583
1107	34-35	0.20506466282947855
1107	36-37	0.21629188509579933
1107	38-39	0.26652256968399257
1107	40-41	0.2900582726119296
1107	42-43	0.2650863598964861
1107	44-45	0.2331656997300655
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2216	84-85	-0.7436173084700926
2216	86-87	-0.8628975718184435
2216	88-89	-0.8694574281371743
2216	90-91	-0.9004720643641981
2216	92-93	-0.8535802241396198
2216	94-95	-0.8129321406179422
2216	96-97	-0.8301666458731631
2216	98-99	-0.846664982006704
2216	100	-0.9101604684830065
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93233.0
3	17409.0
4	11146.0
5	9143.0
6	7923.0
7	8048.0
8	8532.0
9	9857.0
10	11352.0
11	12725.0
12	14338.0
13	15902.0
14	18297.0
15	19192.0
16	19564.0
17	20780.0
18	20095.0
19	18168.0
20	18285.0
21	19984.0
22	22721.0
23	26526.0
24	32856.0
25	40508.0
26	49263.0
27	58151.0
28	70156.0
29	88757.0
30	108826.0
31	127876.0
32	164128.0
33	219771.0
34	305925.0
35	451725.0
36	759134.0
37	1402393.0
38	2008879.0
39	796977.0
40	1956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76095770949614	20.719422172028747	14.644039818988222	22.87558029948689
2	15.273950367506403	20.518638628456205	40.82955624670865	23.37785475732875
3	18.1558673157563	25.67155218723129	30.549711438875526	25.62286905813688
4	12.522861420723984	17.54569998530174	39.39173113858068	30.539707455393593
5	12.99555781597518	37.930805560951086	35.71142949209056	13.362207130983176
6	30.0648907425663	38.337549724323	18.27638474583018	13.321174787280532
7	26.359237483649338	32.70593037795112	23.12982862245414	17.8050035159454
8	24.231834499892823	36.44284589881342	21.15769566384183	18.167623937451932
9	24.43043844625406	17.423135439183966	22.242222753881926	35.90420336068006
10-11	23.223674281291242	27.418316930212317	29.543333510091607	19.81467527840483
12-13	23.80362717731562	25.02413359242747	29.416890490527663	21.755348739729243
14-15	21.35952645344319	27.34165241980912	26.57086636599995	24.727954760747732
16-17	20.857589679706866	30.54903988155113	27.236108873320035	21.357261565421965
18-19	20.743462733120182	29.522499202515046	29.166999538105713	20.567038526259054
20-21	21.880797652962393	28.77524875667376	28.690264570596373	20.653689019767473
22-23	21.56739877169967	28.65119609694016	28.709786723368268	21.0716184079919
24-25	21.360440827923792	28.773650193055623	28.675462595033302	21.190446383987286
26-27	21.1746215253751	28.703277863102173	28.75856525628259	21.36353535524014
28-29	21.158136506243526	28.74328966191071	28.878050248778226	21.22052358306754
30-31	21.447338476897706	29.062027144224682	28.484796741815366	21.005837637062243
32-33	21.11345629663324	28.855669959537817	28.818530041277985	21.21234370255096
34-35	21.34791968038684	28.566522898881765	28.80625386348085	21.279303557250547
36-37	21.578814230338647	28.879369727797172	28.34613042491558	21.195685616948605
38-39	21.40315276616608	28.992224814130584	28.504905092993262	21.099717326710078
40-41	21.34815941104529	28.674894103631104	28.662657033607637	21.31428945171597
42-43	21.246641209633594	28.74182204123173	28.594492023327795	21.417044725806882
44-45	21.436677334108687	28.836465307533132	28.448858839716813	21.27799851864137
46-47	21.452049233480363	28.891473040503037	28.33162379841404	21.324853927602554
48-49	21.334932200528893	28.76630985202273	28.41975890395544	21.478999043492937
50-51	21.354244281767688	28.69444598966502	28.414780210208917	21.53652951835838
52-53	21.39498513620324	28.71416466277983	28.37434840126365	21.516501799753282
54-55	21.510073965082753	28.98620654200632	28.22353063380086	21.280188859110066
56-57	21.389394369171978	28.791041753894397	28.283271736752102	21.53629214018152
58-59	21.307289865813093	28.487280537149857	28.57125678949266	21.634172807544388
60-61	21.50928694595484	28.82747208852908	28.24351826587417	21.41972269964191
62-63	21.373448856302073	28.858428849553214	28.28628098401668	21.48184131012803
64-65	21.425811239005586	28.642330375700283	28.374560838969014	21.557297546325113
66-67	21.272497121301022	28.694367863163183	28.313295844314162	21.719839171221633
68-69	21.649709370303142	28.76075334098087	28.257073499012197	21.332463789703784
70-71	21.59867333235987	28.58000365189506	28.338297716102577	21.48302529964249
72-73	21.396859201408912	28.7331094862583	28.454597117464854	21.415434194867927
74-75	21.498104734134575	28.737286062555096	28.28612740220922	21.478481801101108
76-77	21.51170706390185	28.729846507853235	28.128452096469523	21.629994331775393
78-79	21.59271537290758	28.7546150257319	28.27858643532311	21.374083166037405
80-81	21.455782698559606	28.67417479016727	28.34024857451176	21.52979393676136
82-83	21.419333441074688	28.75915010354282	28.433295792643744	21.38822066273875
84-85	21.4298890543057	28.626664366506393	28.3403281694829	21.603118409705008
86-87	21.552438419288624	28.804795993398756	28.197595603203844	21.44516998410877
88-89	21.37606398739247	28.699712444108332	28.275731547266187	21.648492021233015
90-91	21.32196917828812	28.664158287409798	28.34586010873113	21.66801242557095
92-93	21.42721717987836	28.853011836033115	28.180337020258655	21.539433963829865
94-95	21.29536657745351	28.653472121855955	28.352299480510073	21.69886182018046
96-97	21.394817351374986	28.66506369671109	28.320869865672204	21.61924908624172
98-99	21.33328673063707	28.731047494523715	28.23724645015448	21.698419324684735
100	21.413353391512555	28.63386190461188	28.228699248830942	21.724085455044627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2139.0
1	1609.0
2	1276.0
3	1514.5
4	1430.5
5	1291.5
6	1178.5
7	1104.5
8	1176.5
9	1211.0
10	1126.0
11	1020.0
12	1007.5
13	1144.5
14	1276.0
15	1357.0
16	1688.0
17	2254.5
18	3059.5
19	4182.5
20	5917.0
21	8751.0
22	12969.0
23	19147.5
24	27989.5
25	40117.5
26	55588.0
27	73684.0
28	95371.0
29	120229.0
30	143096.0
31	163549.0
32	182771.5
33	200297.0
34	217471.0
35	230674.5
36	240658.5
37	249477.0
38	258089.5
39	262615.0
40	263857.0
41	263912.5
42	260160.0
43	254733.0
44	247399.0
45	241270.0
46	235928.0
47	227956.5
48	220838.0
49	215160.5
50	207344.5
51	197013.5
52	183335.5
53	170882.5
54	162225.0
55	154613.0
56	142371.0
57	127414.0
58	115087.5
59	103991.5
60	90908.5
61	76630.0
62	64582.5
63	53615.0
64	43575.5
65	34747.5
66	27052.5
67	20804.0
68	16212.5
69	12472.5
70	9364.0
71	6944.0
72	5197.0
73	3904.0
74	2820.5
75	1972.5
76	1332.0
77	924.0
78	652.0
79	459.0
80	323.5
81	219.0
82	170.5
83	135.5
84	98.0
85	64.5
86	51.5
87	50.0
88	39.0
89	30.5
90	26.0
91	23.0
92	23.5
93	18.0
94	17.5
95	17.5
96	10.5
97	6.0
98	9.0
99	13.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07803950804591687
2	0.013571476890306322
3	0.017748397757063814
4	0.011475984603616539
5	0.009985231701676155
6	0.007735038642143501
7	0.020533011668235474
8	0.00991491316856576
9	0.009703957569234572
10-11	0.013782432489637508
12-13	0.015695096590240265
14-15	0.010491525140071001
16-17	0.01315659754495499
18-19	0.00912734559772933
20-21	0.013262075344620584
22-23	0.014091834035323249
24-25	0.016187326322013033
26-27	0.005358272223012134
28-29	0.012031501015188663
30-31	0.010899372632111297
32-33	0.012657335959871183
34-35	0.009225791544083884
36-37	0.010512620700004121
38-39	0.01983685819044256
40-41	0.013444903530707611
42-43	0.008614020306023444
44-45	0.02090569989372057
46-47	0.01905632247291717
48-49	0.0182968823153249
50-51	0.010751703712579466
52-53	0.010920468192044414
54-55	0.012129946961543216
56-57	0.0207509991208777
58-59	0.002215033792977457
60-61	0.007327191150103206
62-63	0.016173262615390954
64-65	0.024977142960812465
66-67	0.013395680557530334
68-69	0.014408267434320029
70-71	0.02620068543693335
72-73	0.020828349507299133
74-75	0.020701776147700423
76-77	0.009718021275856652
78-79	0.011869768389034753
80-81	0.020329087922215328
82-83	0.01240418924067376
84-85	0.011975246188700345
86-87	0.017347582118334558
88-89	0.01547710913759804
90-91	0.015884956629638333
92-93	0.008466351386491613
94-95	0.01580057438990586
96-97	0.013916037702547259
98-99	0.010618098499669713
100	0.0072709363236148905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7110501.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.23048790919231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85699509060345	46.58387327904266
2	15.440351547638123	19.217212205979457
3	4.724846938870518	8.82088590906498
4	1.8773308474826709	4.673088584232964
5	0.9603468835843721	2.988142756376389
6	0.5405388127370911	2.018279643029083
7	0.3498448528920209	1.5239711121593047
8	0.2316084450175227	1.1530485229863823
9	0.16417905916319558	0.9195248660578031
>10	0.799313288877982	8.624491310819772
>50	0.039909650117733037	1.677199753077474
>100	0.014100704249988131	1.4968257580788924
>500	5.207055697682049E-4	0.19767309508365605
>1k	1.131731956633687E-4	0.10578320401122654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.4063706622079091E-5	0.0
3	0.0	0.0	0.0	2.8127413244158182E-5	0.0
4	0.0	0.0	0.0	5.6254826488316364E-5	0.0
5	0.0	1.4063706622079091E-5	0.0	5.6254826488316364E-5	0.0
6	0.0	1.4063706622079091E-5	0.0	7.031853311039546E-5	0.0
7	0.0	2.8127413244158182E-5	0.0	8.438223973247455E-5	0.0
8	0.0	2.8127413244158182E-5	0.0	8.438223973247455E-5	0.0
9	0.0	2.8127413244158182E-5	0.0	1.1250965297663273E-4	0.0
10-11	0.0	2.8127413244158182E-5	0.0	1.1954150628767228E-4	0.0
12-13	0.0	3.515926655519773E-5	0.0	1.687644794649491E-4	0.0
14-15	0.0	4.219111986623727E-5	0.0	2.1095559933118638E-4	0.0
16-17	0.0	4.219111986623727E-5	0.0	2.461148658863841E-4	0.0
18-19	0.0	4.219111986623727E-5	0.0	2.8830598575262133E-4	0.0
20-21	0.0	4.219111986623727E-5	0.0	3.0940154568574003E-4	0.0
22-23	0.0	4.922297317727682E-5	0.0	3.5862451886301683E-4	0.0
24-25	0.0	5.6254826488316364E-5	0.0	5.695801181942032E-4	0.0
26-27	0.0	5.6254826488316364E-5	0.0	7.735038642143501E-4	0.0
28-29	0.0	6.32866797993559E-5	0.0	0.0014204343688299882	0.0
30-31	0.0	7.031853311039546E-5	0.0	0.0029182191240814115	0.0
32-33	0.0	7.031853311039546E-5	0.0	0.006694324352109648	0.0
34-35	0.0	7.031853311039546E-5	0.0	0.013044087891978357	0.0
36-37	0.0	7.031853311039546E-5	0.0	0.022361293529105757	0.0
38-39	0.0	7.031853311039546E-5	0.0	0.033949787785698925	0.0
40-41	0.0	7.031853311039546E-5	0.0	0.045594536868780414	0.0
42-43	0.0	7.031853311039546E-5	0.0	0.05818858614885224	0.0
44-45	0.0	8.438223973247455E-5	0.0	0.07455874065695231	0.0
46-47	0.0	9.844594635455364E-5	0.0	0.09263060366632393	0.0
48-49	0.0	9.844594635455364E-5	0.0	0.11148300239322095	0.0
50-51	0.0	9.844594635455364E-5	0.0	0.13095420421148946	0.0
52-53	0.0	9.844594635455364E-5	0.0	0.14986988961818581	0.0
54-55	0.0	9.844594635455364E-5	0.0	0.17140142445658893	0.0
56-57	0.0	9.844594635455364E-5	0.0	0.1986357923302451	0.0
58-59	0.0	9.844594635455364E-5	0.0	0.22832427700945404	0.0
60-61	0.0	9.844594635455364E-5	0.0	0.2589269026190981	7.0318533110395455E-6
62-63	0.0	1.1250965297663273E-4	0.0	0.29175862572834177	1.4063706622079091E-5
64-65	0.0	1.1250965297663273E-4	0.0	0.32624986621899077	1.4063706622079091E-5
66-67	0.0	1.1250965297663273E-4	0.0	0.3675901318345922	2.8127413244158182E-5
68-69	0.0	1.1954150628767228E-4	0.0	0.41367689843514543	2.8127413244158182E-5
70-71	0.0	1.2657335959871183E-4	0.0	0.463567897676971	2.8127413244158182E-5
72-73	0.0	1.2657335959871183E-4	0.0	0.5182827482901697	2.8127413244158182E-5
74-75	0.0	1.2657335959871183E-4	0.0	0.5746219570182185	2.8127413244158182E-5
76-77	0.0	1.406370662207909E-4	0.0	0.6364108520623231	2.8127413244158182E-5
78-79	0.0	1.406370662207909E-4	0.0	0.7091694382716491	2.8127413244158182E-5
80-81	0.0	1.406370662207909E-4	0.0	0.7885028073267973	2.8127413244158182E-5
82-83	0.0	1.406370662207909E-4	0.0	0.873419467910911	2.8127413244158182E-5
84-85	0.0	1.406370662207909E-4	0.0	0.9624146034154274	2.8127413244158182E-5
86-87	0.0	1.406370662207909E-4	0.0	1.060487861544496	2.8127413244158182E-5
88	0.0	1.406370662207909E-4	0.0	1.1425636533909496	2.8127413244158182E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7015	0.0	44.24623	1
GTATCAA	11945	0.0	33.858856	1
TCAACGC	12615	0.0	30.325207	4
ATCAACG	12725	0.0	30.064545	3
CAACGCA	13720	0.0	28.021427	5
TATCAAC	14590	0.0	27.026804	2
AACGCAG	14315	0.0	26.857857	6
ACGCAGA	16155	0.0	23.333662	7
GTACATG	19850	0.0	22.767895	1
CGCAGAG	16495	0.0	22.651804	8
TACATGG	20120	0.0	21.584005	2
ACATGGG	20700	0.0	20.29809	3
GCAGAGT	19195	0.0	18.583977	9
GTGGTAT	4260	0.0	17.773636	1
CATGGGG	13810	0.0	17.662018	4
AGAGTAC	16850	0.0	17.335339	10-11
CAGAGTA	18495	0.0	16.619366	10-11
GAGTACA	15430	0.0	15.544051	1
TGGTATC	4520	0.0	15.077092	2
GAGTACT	7960	0.0	14.848754	12-13
>>END_MODULE