FastQCFastQC Report
Wed 25 May 2016
SRR2049477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110501
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181950.25588914198872903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180900.25441245279341074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101450.14267630368099238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115800.056.2887651
GTATCAA212700.041.088941
ATCAACG260750.032.988093
TCAACGC262750.032.71914
CAACGCA274500.031.3185585
TATCAAC276800.031.2111532
AACGCAG287550.029.9596526
GTGGTAT50500.029.822161
TGGTATC53900.026.7719522
ACGCAGA325200.026.3465527
CGCAGAG329000.025.8851078
GCAGAGT360400.023.3820829
GTACATG178050.022.1240181
GAGTACT262700.020.94107612-13
TACATGG182450.020.7129782
CAGAGTA353750.020.59315710-11
AGAGTAC337950.020.49204610-11
ACATGGG187250.019.4038563
AGTACTT278550.019.03240212-13
GTACTTT290150.018.86273214-15
Produced by FastQC (version 0.11.5)