FastQCFastQC Report
Wed 25 May 2016
SRR2049476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6771746
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70800.044.370851
GTATCAA118100.032.971221
ATCAACG119100.031.216563
TCAACGC119400.030.979524
CAACGCA129700.028.5913645
TATCAAC137900.027.4379652
AACGCAG137950.026.983696
ACGCAGA151400.024.276277
CGCAGAG154000.023.5279128
GTACATG177050.022.869771
TACATGG177550.021.3370652
GTGGTAT40500.021.2495961
ACATGGG181250.019.9419963
GCAGAGT184100.019.0175889
TGGTATC44250.017.4201092
CAGAGTA178400.017.0438310-11
AGAGTAC160300.016.43235810-11
ACCGCGT3151.5164369E-716.410888
GAGTACA138300.015.6079221
CATGGGG128100.015.4834444
Produced by FastQC (version 0.11.5)