##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049476_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6771746 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.933287515509296 31.0 31.0 34.0 28.0 34.0 2 31.151190549675075 31.0 31.0 34.0 28.0 34.0 3 31.26855392981367 33.0 31.0 34.0 28.0 34.0 4 34.86914763194012 37.0 35.0 37.0 32.0 37.0 5 34.8998596521488 37.0 35.0 37.0 32.0 37.0 6 34.91817664159288 37.0 35.0 37.0 32.0 37.0 7 34.802137735231064 37.0 35.0 37.0 32.0 37.0 8 34.78410088033426 37.0 35.0 37.0 32.0 37.0 9 36.382193602654326 39.0 37.0 39.0 32.0 39.0 10-11 36.32735833564933 39.0 37.0 39.0 32.0 39.0 12-13 36.25469376730905 39.0 37.0 39.0 32.0 39.0 14-15 37.50053117763129 40.0 38.0 41.0 32.0 41.0 16-17 37.49405515209814 40.0 38.0 41.0 32.0 41.0 18-19 37.52060229370682 40.0 38.0 41.0 32.0 41.0 20-21 37.571803490562104 40.0 38.0 41.0 32.0 41.0 22-23 37.53593334717516 40.0 38.0 41.0 32.0 41.0 24-25 37.54151477329481 40.0 38.0 41.0 32.0 41.0 26-27 37.53975747170671 40.0 38.0 41.0 32.0 41.0 28-29 37.51711013673578 40.0 38.0 41.0 32.0 41.0 30-31 37.45698074026994 40.0 38.0 41.0 32.0 41.0 32-33 37.40685282643501 40.0 38.0 41.0 32.0 41.0 34-35 37.33601599941876 40.0 38.0 41.0 31.0 41.0 36-37 37.22762563746484 40.0 37.5 41.0 31.0 41.0 38-39 37.12225598833743 40.0 37.0 41.0 31.0 41.0 40-41 37.001890797439835 40.0 37.0 41.0 30.0 41.0 42-43 36.91167720998395 40.0 37.0 41.0 30.0 41.0 44-45 36.77003087534588 40.0 37.0 41.0 30.0 41.0 46-47 36.653788550249814 40.0 37.0 41.0 30.0 41.0 48-49 36.55702081855994 40.0 36.0 41.0 30.0 41.0 50-51 36.4509557800898 40.0 36.0 41.0 30.0 41.0 52-53 36.34417637637324 40.0 36.0 41.0 30.0 41.0 54-55 36.20458763515347 39.5 35.5 41.0 29.5 41.0 56-57 36.0473120964667 39.0 35.0 41.0 29.0 41.0 58-59 35.871041899681416 39.0 35.0 41.0 29.0 41.0 60-61 35.68441004727585 39.0 35.0 41.0 28.5 41.0 62-63 35.437416066698304 39.0 35.0 40.5 28.0 41.0 64-65 35.20083247363383 38.0 35.0 40.0 28.0 41.0 66-67 34.9755656812881 38.0 34.0 40.0 28.0 41.0 68-69 34.69029516169094 37.0 34.0 40.0 28.0 41.0 70-71 34.377880165617555 37.0 34.0 39.5 27.5 41.0 72-73 34.04090503394546 36.0 34.0 39.0 26.0 41.0 74-75 33.73521119959313 36.0 34.0 39.0 26.0 41.0 76-77 33.400567076792306 35.0 34.0 38.5 26.0 40.0 78-79 33.04661397518454 35.0 33.0 37.0 26.0 39.5 80-81 32.70183694131469 35.0 33.0 37.0 26.0 39.0 82-83 32.386380779787075 35.0 33.0 36.5 25.0 39.0 84-85 32.086814242589725 35.0 33.0 36.0 25.0 37.5 86-87 31.799655731328375 35.0 33.0 36.0 25.0 37.0 88-89 31.55379868057662 35.0 33.0 35.5 24.0 37.0 90-91 31.327696431614534 35.0 33.0 35.0 23.5 36.0 92-93 31.104692349653988 35.0 32.0 35.0 22.0 36.0 94-95 30.885569098427496 35.0 32.0 35.0 20.0 36.0 96-97 30.679925974778143 35.0 32.0 35.0 18.0 36.0 98-99 30.49279890297126 35.0 32.0 35.0 7.5 35.0 100 30.352705934333628 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30501842185131167 1101 2 0.32422108211580536 1101 3 0.35292364752946526 1101 4 0.4108883412726172 1101 5 0.4122737880678855 1101 6 0.43516869621241483 1101 7 0.4713227602227832 1101 8 0.4530315814322847 1101 9 0.5162693656207722 1101 10-11 0.5636807743328234 1101 12-13 0.5156273967157361 1101 14-15 0.636531598996676 1101 16-17 0.6423475526312288 1101 18-19 0.6326111044675002 1101 20-21 0.6556919659114229 1101 22-23 0.607467421323463 1101 24-25 0.6747242600128871 1101 26-27 0.6512622062922233 1101 28-29 0.6216302024168456 1101 30-31 0.5919801720233124 1101 32-33 0.6323159720985672 1101 34-35 0.6329404297419927 1101 36-37 0.6005399062190051 1101 38-39 0.6388185972350868 1101 40-41 0.6825124743477602 1101 42-43 0.5342065675363017 1101 44-45 0.5980001558486734 1101 46-47 0.5848623815914209 1101 48-49 0.5370335880930526 1101 50-51 0.5679058723603987 1101 52-53 0.5727946180510486 1101 54-55 0.4914562207730384 1101 56-57 0.49719753679175227 1101 58-59 0.5420423641483936 1101 60-61 0.5480626752539592 1101 62-63 0.46861359062714314 1101 64-65 0.5392515554020463 1101 66-67 0.4833459542872731 1101 68-69 0.42475329656291905 1101 70-71 0.416989675376108 1101 72-73 0.4249319996086953 1101 74-75 0.38425969632385204 1101 76-77 0.35667137213770417 1101 78-79 0.36519078470524136 1101 80-81 0.41648730680142165 1101 82-83 0.37507317219849057 1101 84-85 0.3774132074335341 1101 86-87 0.37001266189524884 1101 88-89 0.38048777928571553 1101 90-91 0.37255561120008807 1101 92-93 0.32009712855585803 1101 94-95 0.3140026996500964 1101 96-97 0.3125197328750886 1101 98-99 0.41040376743638163 1101 100 0.35075190384982236 1102 1 0.33760769490077536 1102 2 0.32679946952314864 1102 3 0.3796176784703533 1102 4 0.4321436650464534 1102 5 0.3605046851475677 1102 6 0.3863969912438776 1102 7 0.4063031696577113 1102 8 0.4753680144758121 1102 9 0.5111417685217035 1102 10-11 0.5705071291864883 1102 12-13 0.5133047601897687 1102 14-15 0.6132391082824782 1102 16-17 0.5249100116691778 1102 18-19 0.5680873626649259 1102 20-21 0.5531860852462742 1102 22-23 0.5537093529538666 1102 24-25 0.599626937350088 1102 26-27 0.5569053084698012 1102 28-29 0.5664145383162165 1102 30-31 0.5169809288128846 1102 32-33 0.5141958699627622 1102 34-35 0.5297107895393722 1102 36-37 0.5081120965125905 1102 38-39 0.5681361697033651 1102 40-41 0.5510183845948688 1102 42-43 0.520295479288599 1102 44-45 0.45127379447698246 1102 46-47 0.4599640190842962 1102 48-49 0.4384256552405077 1102 50-51 0.49262146275577834 1102 52-53 0.4816499679445201 1102 54-55 0.47357485869640215 1102 56-57 0.41268616805274405 1102 58-59 0.4795531735170613 1102 60-61 0.4712225964108754 1102 62-63 0.4435665727715943 1102 64-65 0.42911203408161214 1102 66-67 0.43118420064141105 1102 68-69 0.4175261702095483 1102 70-71 0.3142744856214321 1102 72-73 0.40666744518987485 1102 74-75 0.44941839230206426 1102 76-77 0.39226339357578865 1102 78-79 0.39439900375621306 1102 80-81 0.40920879247457975 1102 82-83 0.34866760114292106 1102 84-85 0.3887068084703813 1102 86-87 0.34485088905914907 1102 88-89 0.3566210559091765 1102 90-91 0.4002671644411784 1102 92-93 0.35510959661939623 1102 94-95 0.3519421301367558 1102 96-97 0.3530122788723773 1102 98-99 0.37506259825749666 1102 100 0.3905826126987648 1103 1 0.289874281146993 1103 2 0.3256874679354915 1103 3 0.3645386973502731 1103 4 0.3527254493752565 1103 5 0.39374577652568377 1103 6 0.3814905847202894 1103 7 0.40686966959428617 1103 8 0.40640222575378004 1103 9 0.4625914862949898 1103 10-11 0.5041009248295438 1103 12-13 0.46414931667750636 1103 14-15 0.5530828093424844 1103 16-17 0.5513993374726525 1103 18-19 0.5271981137485326 1103 20-21 0.5288933094301669 1103 22-23 0.49872222838557434 1103 24-25 0.5462616325483438 1103 26-27 0.5186367783109915 1103 28-29 0.5116804028158626 1103 30-31 0.5229334265844585 1103 32-33 0.6029625200660078 1103 34-35 0.5243632091510477 1103 36-37 0.5443574628512167 1103 38-39 0.5840472376648194 1103 40-41 0.5773641880812761 1103 42-43 0.47483184860267613 1103 44-45 0.5229759513469006 1103 46-47 0.5142955966917242 1103 48-49 0.5516463232618847 1103 50-51 0.5518160833285606 1103 52-53 0.4896221411663362 1103 54-55 0.4529995962013871 1103 56-57 0.46456723099571207 1103 58-59 0.44036108748612435 1103 60-61 0.44772630337400443 1103 62-63 0.4837383802670203 1103 64-65 0.5344881521722442 1103 66-67 0.5130656138961314 1103 68-69 0.4933649042391437 1103 70-71 0.49794992046575004 1103 72-73 0.5337255295785681 1103 74-75 0.5282289225826275 1103 76-77 0.4237595739411475 1103 78-79 0.46905197560805334 1103 80-81 0.4441126054660103 1103 82-83 0.3689297838524155 1103 84-85 0.36829142676227633 1103 86-87 0.35491400257692973 1103 88-89 0.37483421548364504 1103 90-91 0.3651936862105245 1103 92-93 0.3518629281601804 1103 94-95 0.3945479009738335 1103 96-97 0.3898868904296222 1103 98-99 0.3631839895387792 1103 100 0.3042179917536636 1104 1 0.13501128743843438 1104 2 0.10560322900808927 1104 3 0.21738932296745261 1104 4 0.24640233848170112 1104 5 0.30759134104499 1104 6 0.27337541777524876 1104 7 0.31602912666124183 1104 8 0.2587892209162632 1104 9 0.40290850256615585 1104 10-11 0.3813686704271646 1104 12-13 0.4161433954188354 1104 14-15 0.4636233016284308 1104 16-17 0.4170653994405171 1104 18-19 0.44878165510890256 1104 20-21 0.4201444880880487 1104 22-23 0.4424468164476565 1104 24-25 0.4352010447574415 1104 26-27 0.42693165781080467 1104 28-29 0.40588172369596265 1104 30-31 0.4259231435137991 1104 32-33 0.3823218440902423 1104 34-35 0.42845397497874416 1104 36-37 0.4352476846719284 1104 38-39 0.42292781108098154 1104 40-41 0.46512842360754547 1104 42-43 0.4581342768652803 1104 44-45 0.4333644457748349 1104 46-47 0.43621568298021174 1104 48-49 0.3943274523383593 1104 50-51 0.37561276223845397 1104 52-53 0.39954024943746447 1104 54-55 0.35298797552346173 1104 56-57 0.35297611933263795 1104 58-59 0.43531566588744397 1104 60-61 0.3845915692612536 1104 62-63 0.37839664117219485 1104 64-65 0.4175721438909932 1104 66-67 0.3894778004081658 1104 68-69 0.34411060103096247 1104 70-71 0.3744797279941636 1104 72-73 0.31907456135046885 1104 74-75 0.32646867163690274 1104 76-77 0.30431762931197426 1104 78-79 0.3092863112235804 1104 80-81 0.310887723090282 1104 82-83 0.36774963558205087 1104 84-85 0.34209236796539244 1104 86-87 0.33015262463649364 1104 88-89 0.29667429946409385 1104 90-91 0.32596676176338235 1104 92-93 0.32528124206139 1104 94-95 0.3270175872355452 1104 96-97 0.34288220556234705 1104 98-99 0.3446157628002702 1104 100 0.36757168241439686 1105 1 0.08586846436064377 1105 2 0.12518653101722066 1105 3 0.16450521584808797 1105 4 0.16913777507839 1105 5 0.16982472316576747 1105 6 0.174819894065962 1105 7 0.18712148175549714 1105 8 0.22539047873947737 1105 9 0.23365166723434072 1105 10-11 0.27310871403044956 1105 12-13 0.25684442512357464 1105 14-15 0.3120982878849432 1105 16-17 0.22121534681586752 1105 18-19 0.25047275073541186 1105 20-21 0.2501021005732511 1105 22-23 0.2746534574911266 1105 24-25 0.27618553399288004 1105 26-27 0.3046418862581888 1105 28-29 0.30966986188736456 1105 30-31 0.2969784968977507 1105 32-33 0.3469007088307805 1105 34-35 0.28902646485319394 1105 36-37 0.2843612440954999 1105 38-39 0.29294080279260015 1105 40-41 0.2541051001961563 1105 42-43 0.24206345030580678 1105 44-45 0.25466270364034216 1105 46-47 0.1486706201830188 1105 48-49 0.15066428889181793 1105 50-51 0.11645491235498184 1105 52-53 0.1440959323128368 1105 54-55 0.10890604668890802 1105 56-57 0.08784506279872772 1105 58-59 0.05043951296114102 1105 60-61 0.07828898865624723 1105 62-63 0.11521837926586898 1105 64-65 0.11426596765949171 1105 66-67 0.09567240062524007 1105 68-69 0.06856187447712614 1105 70-71 0.09394413998412432 1105 72-73 0.03361825978300459 1105 74-75 0.09565016737524701 1105 76-77 0.010719703404667769 1105 78-79 0.021761070771198376 1105 80-81 0.037729644735932766 1105 82-83 0.035519648221367106 1105 84-85 0.020675176031055287 1105 86-87 0.03487537904380744 1105 88-89 0.021470701958122618 1105 90-91 0.07708364076896146 1105 92-93 0.053860922601540295 1105 94-95 0.10217904390478338 1105 96-97 0.10774081497934063 1105 98-99 0.10485965693073851 1105 100 0.012411793912882274 1106 1 0.2063894074688477 1106 2 0.19401634380586685 1106 3 0.22708762771823743 1106 4 0.26377840443709744 1106 5 0.20898408334615937 1106 6 0.2282857450407647 1106 7 0.28833899807966645 1106 8 0.2441350222826486 1106 9 0.3243535580742076 1106 10-11 0.33038665883822205 1106 12-13 0.3463324964694934 1106 14-15 0.41587505196712016 1106 16-17 0.38599550003576155 1106 18-19 0.35839581663132947 1106 20-21 0.3198494214206633 1106 22-23 0.3354839108484313 1106 24-25 0.3747952917520294 1106 26-27 0.36603203002452744 1106 28-29 0.32343688183539143 1106 30-31 0.36206942600643544 1106 32-33 0.3785657950621726 1106 34-35 0.36381023257214906 1106 36-37 0.3649265152652603 1106 38-39 0.3632564507484659 1106 40-41 0.41918782040454516 1106 42-43 0.37542058702847214 1106 44-45 0.32415588758944835 1106 46-47 0.4023433564314587 1106 48-49 0.30273549855923676 1106 50-51 0.34271936550823057 1106 52-53 0.3540270953969724 1106 54-55 0.31441469501154273 1106 56-57 0.29738834121879165 1106 58-59 0.3150099073887134 1106 60-61 0.2940826141586328 1106 62-63 0.35249769141652365 1106 64-65 0.35881310272907285 1106 66-67 0.4268148777533227 1106 68-69 0.4091279592723964 1106 70-71 0.419491811659114 1106 72-73 0.41057879055096436 1106 74-75 0.3477621632502945 1106 76-77 0.32371130917857016 1106 78-79 0.32947750968730105 1106 80-81 0.3458310625106762 1106 82-83 0.32221875981979053 1106 84-85 0.2927411762351184 1106 86-87 0.3176077837463076 1106 88-89 0.32864234271792014 1106 90-91 0.26872251663553826 1106 92-93 0.2121738022934494 1106 94-95 0.2648933309105388 1106 96-97 0.3500068928006854 1106 98-99 0.3746422187404512 1106 100 0.35948611602076497 1107 1 0.15777965937675376 1107 2 0.202259617743902 1107 3 0.23001994796289793 1107 4 0.19454145959359437 1107 5 0.12755683965846032 1107 6 0.19277436098812473 1107 7 0.18281909897069681 1107 8 0.1491589506787392 1107 9 0.13427503207785918 1107 10-11 0.14299120714203184 1107 12-13 0.18301311325127045 1107 14-15 0.2549821387654063 1107 16-17 0.251378234204914 1107 18-19 0.22444248996301042 1107 20-21 0.2958632729271571 1107 22-23 0.3124805448819714 1107 24-25 0.30704010239010415 1107 26-27 0.35938106337340514 1107 28-29 0.3369548528336992 1107 30-31 0.3440842559020538 1107 32-33 0.31915896791360865 1107 34-35 0.38081908017239385 1107 36-37 0.37222646877665255 1107 38-39 0.3460564630480292 1107 40-41 0.4122984612364604 1107 42-43 0.42027415578466787 1107 44-45 0.3031481434553598 1107 46-47 0.30615740009712766 1107 48-49 0.355048120980598 1107 50-51 0.4222462481572222 1107 52-53 0.33537814321267945 1107 54-55 0.3222170478422086 1107 56-57 0.36212517323769333 1107 58-59 0.3778965319053924 1107 60-61 0.36686567469050146 1107 62-63 0.38624910702549897 1107 64-65 0.3771472073380835 1107 66-67 0.36710490568496823 1107 68-69 0.33038559142620727 1107 70-71 0.3743911813886527 1107 72-73 0.2993891757623288 1107 74-75 0.3213479346433985 1107 76-77 0.330183667977586 1107 78-79 0.33916370042817334 1107 80-81 0.307470302747582 1107 82-83 0.3243201866169727 1107 84-85 0.3061342665474882 1107 86-87 0.31209740384433005 1107 88-89 0.24743284990865533 1107 90-91 0.3359400956263059 1107 92-93 0.28864912916864327 1107 94-95 0.26928516471900465 1107 96-97 0.3202853190024406 1107 98-99 0.4079151557684604 1107 100 0.3663238146409462 1108 1 0.1502168675643425 1108 2 0.099265775521026 1108 3 0.13263834920660855 1108 4 0.11677262078072204 1108 5 0.14124140484335612 1108 6 0.16899144477506667 1108 7 0.1421926390319186 1108 8 0.17029315302730197 1108 9 0.20925969207485906 1108 10-11 0.1963585866206472 1108 12-13 0.25747611963001305 1108 14-15 0.2562621088829573 1108 16-17 0.21932781452407113 1108 18-19 0.22636895787739775 1108 20-21 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-0.5458152707642725 2215 48-49 -0.5138637382281246 2215 50-51 -0.5667628349712217 2215 52-53 -0.5434846396886996 2215 54-55 -0.6115449177877892 2215 56-57 -0.5792015854060182 2215 58-59 -0.6090482878410057 2215 60-61 -0.5127305157364148 2215 62-63 -0.5276298111466602 2215 64-65 -0.5006744687423463 2215 66-67 -0.486525961674225 2215 68-69 -0.5056747804121073 2215 70-71 -0.4853297570746875 2215 72-73 -0.4144874029366292 2215 74-75 -0.4214758591717356 2215 76-77 -0.4974300053424727 2215 78-79 -0.40528545806760263 2215 80-81 -0.44498020056582277 2215 82-83 -0.4105508254226393 2215 84-85 -0.4053209326396825 2215 86-87 -0.42508630449324514 2215 88-89 -0.4032273022435575 2215 90-91 -0.3836539213013168 2215 92-93 -0.3850177538690467 2215 94-95 -0.46294700651538534 2215 96-97 -0.5045616344095052 2215 98-99 -0.4495055550631477 2215 100 -0.4355148199144665 2216 1 -0.36671569845684715 2216 2 -0.5416042781533079 2216 3 -0.5558770998924025 2216 4 -0.6499663787357193 2216 5 -0.5926880387627236 2216 6 -0.6137685196518632 2216 7 -0.5790353256829022 2216 8 -0.5268943266398765 2216 9 -0.650112234946512 2216 10-11 -0.6853474106406594 2216 12-13 -0.7023189773235501 2216 14-15 -0.8717782430521481 2216 16-17 -0.8438111030842066 2216 18-19 -0.9218400270775149 2216 20-21 -0.9081233948762844 2216 22-23 -0.9508697074275858 2216 24-25 -0.9621368771399332 2216 26-27 -0.9789682219141866 2216 28-29 -0.9190653008259915 2216 30-31 -1.030942847769552 2216 32-33 -1.054602385174789 2216 34-35 -1.0157147563570774 2216 36-37 -0.9658370189310617 2216 38-39 -0.9047694979703778 2216 40-41 -0.8955489230162215 2216 42-43 -0.8283781643244907 2216 44-45 -0.9205399424371947 2216 46-47 -0.8224620087973165 2216 48-49 -0.9558008513526346 2216 50-51 -0.9521145463151583 2216 52-53 -1.0341867874922315 2216 54-55 -0.993289639400281 2216 56-57 -1.0274354228037836 2216 58-59 -0.9839982050379774 2216 60-61 -0.9673180367153478 2216 62-63 -0.9834602256123048 2216 64-65 -0.9690605974851749 2216 66-67 -0.9263443853065496 2216 68-69 -0.8593273511597062 2216 70-71 -0.8357883151241978 2216 72-73 -0.8145145357862233 2216 74-75 -0.9077090251349418 2216 76-77 -0.9477627236916888 2216 78-79 -0.9263863033858044 2216 80-81 -0.851811093910321 2216 82-83 -0.8685435800901118 2216 84-85 -0.7799752415451735 2216 86-87 -0.8901239247761019 2216 88-89 -0.9016117544354607 2216 90-91 -0.9049902945451151 2216 92-93 -0.9057685827134812 2216 94-95 -0.8618450220110319 2216 96-97 -0.8790917625248653 2216 98-99 -0.9174625790448161 2216 100 -0.9982097639091165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96095.0 3 18426.0 4 11559.0 5 9209.0 6 8080.0 7 8665.0 8 9403.0 9 11214.0 10 12464.0 11 13803.0 12 15627.0 13 17244.0 14 19820.0 15 20491.0 16 20616.0 17 22110.0 18 21133.0 19 18887.0 20 19101.0 21 20356.0 22 22840.0 23 27484.0 24 32967.0 25 41191.0 26 49742.0 27 57164.0 28 69202.0 29 87448.0 30 106338.0 31 125008.0 32 160293.0 33 213064.0 34 292559.0 35 426085.0 36 713646.0 37 1314044.0 38 1868318.0 39 768163.0 40 1887.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.68451833165864 20.838276255544113 14.69702762573176 22.780177787065487 2 15.166811995410919 20.680413229878173 40.90033815517565 23.252436619535256 3 18.03617932097088 25.927966882737856 30.703786129694706 25.332067666596558 4 12.56592613378329 17.70101277133078 39.4433634494039 30.289697645482033 5 12.874061274672693 38.017618868286775 35.82317626990984 13.285143587130694 6 29.75783467789218 38.59102953342976 18.356292686038987 13.294843102639073 7 25.99811680950605 32.91373879778596 23.34738218058689 17.7407622121211 8 23.93387746735181 36.71418904329944 21.3066630276855 18.045270461663247 9 24.204327835112142 17.680645342801814 22.41865020730017 35.696376614785876 10-11 22.981995524357867 27.593801802781087 29.715729377250078 19.708473295610965 12-13 23.603859820422603 25.31232417422672 29.45448492662423 21.629331078726448 14-15 21.20534418731387 27.63470310193551 26.6604441379127 24.499508572837925 16-17 20.65223748424879 30.75834880639347 27.35842814582623 21.230985563531508 18-19 20.56200456921128 29.724972292538514 29.28423000357175 20.428793134678457 20-21 21.699001444863246 29.010662272557358 28.756640751287538 20.53369553129186 22-23 21.390062755960088 28.942119512361188 28.734929287114092 20.932888444564632 24-25 21.221465842310863 28.99907262187475 28.768586886350466 21.01087464946392 26-27 20.97433823099402 28.920498815901478 28.87361778871004 21.23154516439446 28-29 20.994202809722857 28.94331336691551 29.016663460569248 21.045820362792384 30-31 21.299787529590084 29.315681051734803 28.525287877726097 20.859243540949013 32-33 20.96783864885757 29.108044665113553 28.848153914636736 21.07596277139214 34-35 21.161770880423347 28.81334417735919 28.890856711025858 21.1340282311916 36-37 21.381993564240588 29.115516173321705 28.461735055748544 21.04075520668916 38-39 21.220446814346374 29.189443912476886 28.592520186581037 20.997589086595703 40-41 21.17374359646923 28.92506934850656 28.737619397436724 21.163567657587485 42-43 21.06802337160001 28.922786985102146 28.670217632462858 21.338972010834993 44-45 21.209890908618263 29.04036834570118 28.572713580731595 21.177027164948964 46-47 21.260874901650848 29.11605325498584 28.432066010872724 21.191005832490585 48-49 21.11199361055632 28.995711938888206 28.529360830391358 21.362933620164114 50-51 21.136891802790593 28.91380552827239 28.48916045943427 21.46014220950274 52-53 21.102736227039898 28.971122452551224 28.50283390776512 21.423307412643762 54-55 21.2420640998167 29.2149087627166 28.320472794817757 21.222554342648937 56-57 21.10593496967674 29.013083460603394 28.378421890647203 21.502559679072668 58-59 21.033890401946657 28.68917756972772 28.652657437691126 21.6242745906345 60-61 21.229894997167083 29.007020593628084 28.335465003843062 21.427619405361767 62-63 21.13775965269313 29.012947944880956 28.38492085118777 21.464371551238145 64-65 21.161649089958928 28.788839334642418 28.463218010160507 21.586293565238147 66-67 21.006529050638452 28.86818096728016 28.34689813787473 21.778391844206656 68-69 21.351727389209906 29.00155010984283 28.29664927116185 21.350073229785416 70-71 21.32614500790725 28.810061489192513 28.32582588182675 21.537967621073488 72-73 21.14891958675088 28.91212928402779 28.471320471603175 21.467630657618155 74-75 21.202799214846806 28.909376604641512 28.3380586829564 21.549765497555278 76-77 21.24901881004827 28.932457948210892 28.148502868434523 21.67002037330632 78-79 21.345224344361693 28.926493395285807 28.289430640716574 21.438851619635926 80-81 21.16976252083418 28.833689994816424 28.367183126082697 21.6293643582667 82-83 21.117568435743877 28.891108337825568 28.454123867404128 21.53719935902643 84-85 21.202575344739763 28.737211779038685 28.360471465196234 21.69974141102532 86-87 21.33816666608819 28.882444543680368 28.227290507968878 21.552098282262566 88-89 21.196753417992078 28.75508723071582 28.316716394036913 21.731442957255187 90-91 21.191826791500503 28.65016902961537 28.314753540509134 21.84325063837499 92-93 21.295236798429585 28.819159434322 28.155673099104117 21.7299306681443 94-95 21.202823810623084 28.613340198197857 28.307286578111686 21.876549413067377 96-97 21.268126071783403 28.65151463459266 28.235694797736354 21.844664495887585 98-99 21.268493544381922 28.66948532132028 28.155859649547732 21.90616148475007 100 21.3686543605448 28.585682082399977 28.12312442699029 21.922539130064933 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3654.0 1 2671.5 2 1955.0 3 2254.5 4 2126.0 5 1843.5 6 1695.5 7 1651.0 8 1658.5 9 1698.0 10 1586.5 11 1447.0 12 1466.0 13 1513.0 14 1616.5 15 1871.0 16 2238.5 17 2787.5 18 3643.0 19 4968.0 20 7009.5 21 10039.0 22 14641.0 23 21611.5 24 30896.5 25 43184.5 26 58608.5 27 76075.0 28 96466.0 29 119234.5 30 140450.0 31 158517.5 32 176049.0 33 191757.0 34 204673.0 35 216243.5 36 226407.5 37 235468.5 38 243108.0 39 246205.0 40 246699.0 41 245792.5 42 242206.0 43 237376.5 44 231553.0 45 226694.5 46 221959.5 47 215359.0 48 209826.5 49 205439.0 50 198227.0 51 188885.0 52 176451.0 53 164350.5 54 156969.5 55 150001.5 56 137794.5 57 123016.0 58 109875.5 59 97857.5 60 84921.0 61 70994.0 62 58850.0 63 48001.0 64 38308.5 65 30507.0 66 24091.5 67 18427.5 68 13941.5 69 10622.5 70 7810.0 71 5671.0 72 4193.5 73 2980.5 74 2094.0 75 1466.0 76 948.0 77 627.0 78 446.0 79 297.0 80 212.0 81 162.5 82 129.5 83 101.0 84 78.0 85 56.5 86 40.5 87 40.5 88 35.0 89 28.5 90 21.5 91 16.5 92 16.5 93 16.0 94 16.0 95 11.5 96 7.5 97 9.0 98 10.0 99 9.0 100 19.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07881276114018453 2 0.013142843810148814 3 0.016805119388707136 4 0.010647180210244153 5 0.00880127518072887 6 0.007442689079005621 7 0.01950752435191751 8 0.009155688946395804 9 0.009554404432771104 10-11 0.012906567966370859 12-13 0.015010899700018282 14-15 0.009613473393715596 16-17 0.01237494731787046 18-19 0.008203201951165917 20-21 0.012744128323773514 22-23 0.013512024816051871 24-25 0.015431766046747767 26-27 0.005168534082642793 28-29 0.011717805127363017 30-31 0.010122943181861813 32-33 0.012168205954564746 34-35 0.00860930105765928 36-37 0.009930969058792222 38-39 0.018946369222944865 40-41 0.012877033485898614 42-43 0.007922624386679596 44-45 0.020186817402779136 46-47 0.018089869289249775 48-49 0.017772373624173145 50-51 0.010034339740445078 52-53 0.010440438846938441 54-55 0.011444611182994755 56-57 0.020172050162543014 58-59 0.0020452627727029337 60-61 0.006682176206845325 62-63 0.015047817800608586 64-65 0.02380479126062909 66-67 0.012603839541530352 68-69 0.013814753240892378 70-71 0.025023088580109175 72-73 0.019943157938883117 74-75 0.019898856218174752 76-77 0.00931812858899315 78-79 0.011060662936855576 80-81 0.01950014073179945 82-83 0.011754723227953323 84-85 0.01135600774157802 86-87 0.016605761645519485 88-89 0.014686020414823593 90-91 0.015343162605331034 92-93 0.008033378688450513 94-95 0.015313628124858788 96-97 0.01336435241369065 98-99 0.00984974923749355 100 0.007117809793810931 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6771746.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.66992973248418 #Duplication Level Percentage of deduplicated Percentage of total 1 76.50221789652616 48.70890837851014 2 14.483245317955578 18.44294423385124 3 4.345624688239041 8.300568556317845 4 1.7670287068361914 4.500263743981707 5 0.8732030168597771 2.7798387362827603 6 0.5068874226594445 1.9364091949804103 7 0.32139718210356205 1.432433520052655 8 0.22537815035486344 1.1479848797065126 9 0.16116637572946374 0.9235306636140689 >10 0.7630179796733547 8.401415802139155 >50 0.03647905568870695 1.595298821222374 >100 0.013843184911981566 1.5189023395978356 >500 3.9488910741110436E-4 0.1624044304548844 >1k 1.1613335450433842E-4 0.1490966992884685 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4767240236122265E-5 0.0 5 0.0 1.4767240236122265E-5 0.0 1.4767240236122265E-5 0.0 6 0.0 1.4767240236122265E-5 0.0 1.4767240236122265E-5 0.0 7 0.0 1.4767240236122265E-5 0.0 2.953448047224453E-5 0.0 8 0.0 1.4767240236122265E-5 0.0 2.953448047224453E-5 0.0 9 2.953448047224453E-5 1.4767240236122265E-5 0.0 2.953448047224453E-5 0.0 10-11 2.953448047224453E-5 1.4767240236122265E-5 0.0 3.691810059030566E-5 0.0 12-13 2.953448047224453E-5 2.953448047224453E-5 0.0 4.4301720708366794E-5 0.0 14-15 2.953448047224453E-5 2.953448047224453E-5 0.0 4.4301720708366794E-5 0.0 16-17 2.953448047224453E-5 2.953448047224453E-5 0.0 5.906896094448906E-5 0.0 18-19 3.691810059030566E-5 2.953448047224453E-5 0.0 8.121982129867246E-5 0.0 20-21 4.4301720708366794E-5 2.953448047224453E-5 0.0 1.1075430177091697E-4 0.0 22-23 4.4301720708366794E-5 2.953448047224453E-5 0.0 1.9935774318765058E-4 0.0 24-25 4.4301720708366794E-5 2.953448047224453E-5 0.0 4.0609910649336224E-4 0.0 26-27 4.4301720708366794E-5 2.953448047224453E-5 0.0 0.0010410904366466196 0.0 28-29 4.4301720708366794E-5 2.953448047224453E-5 0.0 0.0028131592649812912 0.0 30-31 4.4301720708366794E-5 2.953448047224453E-5 0.0 0.009059701884861009 0.0 32-33 4.4301720708366794E-5 2.953448047224453E-5 0.0 0.024417631730428165 0.0 34-35 5.906896094448906E-5 2.953448047224453E-5 0.0 0.05096174605485794 0.0 36-37 5.906896094448906E-5 2.953448047224453E-5 0.0 0.09193345408997916 0.0 38-39 5.906896094448906E-5 2.953448047224453E-5 0.0 0.14176550626677376 0.0 40-41 5.906896094448906E-5 2.953448047224453E-5 0.0 0.1939381660209937 0.0 42-43 5.906896094448906E-5 2.953448047224453E-5 0.0 0.2496401961916469 0.0 44-45 5.906896094448906E-5 2.953448047224453E-5 0.0 0.3218741518066389 0.0 46-47 5.906896094448906E-5 4.4301720708366794E-5 0.0 0.4127295973593812 0.0 48-49 7.383620118061132E-5 4.4301720708366794E-5 0.0 0.506671396121473 0.0 50-51 7.383620118061132E-5 1.0337068165285585E-4 0.0 0.6000225052741199 0.0 52-53 1.0337068165285585E-4 1.1813792188897812E-4 0.0 0.694606678986483 0.0 54-55 1.0337068165285585E-4 1.2552154200703926E-4 0.0 0.8065497435964077 0.0 56-57 1.0337068165285585E-4 1.3290516212510038E-4 0.0 0.9444831510219078 0.0 58-59 1.0337068165285585E-4 1.3290516212510038E-4 0.0 1.0968736866385715 0.0 60-61 1.0337068165285585E-4 1.4767240236122263E-4 0.0 1.2531701572976894 0.0 62-63 1.0337068165285585E-4 1.6243964259734492E-4 0.0 1.4113716019472675 0.0 64-65 1.0337068165285585E-4 1.6243964259734492E-4 0.0 1.5847537695595788 0.0 66-67 1.0337068165285585E-4 1.9197412306958943E-4 0.0 1.7892948140701084 0.0 68-69 1.0337068165285585E-4 1.9197412306958943E-4 0.0 2.0190952230045247 0.0 70-71 1.0337068165285585E-4 1.9197412306958943E-4 0.0 2.260318092261582 0.0 72-73 1.0337068165285585E-4 1.9197412306958943E-4 0.0 2.5090796376591795 0.0 74-75 1.3290516212510038E-4 2.1412498342377283E-4 0.0 2.770230897614884 0.0 76-77 1.3290516212510038E-4 2.3627584377795623E-4 0.0 3.054064638573272 0.0 78-79 1.3290516212510038E-4 2.510430840140785E-4 0.0 3.373457894020242 0.0 80-81 1.3290516212510038E-4 2.510430840140785E-4 0.0 3.72149959552529 0.0 82-83 1.3290516212510038E-4 2.510430840140785E-4 0.0 4.081044977174277 0.0 84-85 1.3290516212510038E-4 2.510430840140785E-4 0.0 4.443957289597099 0.0 86-87 1.3290516212510038E-4 2.510430840140785E-4 0.0 4.8329928499976225 0.0 88 1.3290516212510038E-4 2.510430840140785E-4 0.0 5.14988305822457 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7080 0.0 44.37085 1 GTATCAA 11810 0.0 32.97122 1 ATCAACG 11910 0.0 31.21656 3 TCAACGC 11940 0.0 30.97952 4 CAACGCA 12970 0.0 28.591364 5 TATCAAC 13790 0.0 27.437965 2 AACGCAG 13795 0.0 26.98369 6 ACGCAGA 15140 0.0 24.27627 7 CGCAGAG 15400 0.0 23.527912 8 GTACATG 17705 0.0 22.86977 1 TACATGG 17755 0.0 21.337065 2 GTGGTAT 4050 0.0 21.249596 1 ACATGGG 18125 0.0 19.941996 3 GCAGAGT 18410 0.0 19.017588 9 TGGTATC 4425 0.0 17.420109 2 CAGAGTA 17840 0.0 17.04383 10-11 AGAGTAC 16030 0.0 16.432358 10-11 ACCGCGT 315 1.5164369E-7 16.41088 8 GAGTACA 13830 0.0 15.607922 1 CATGGGG 12810 0.0 15.483444 4 >>END_MODULE