FastQCFastQC Report
Wed 25 May 2016
SRR2049476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6771746
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217950.32185200094628474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217470.32114317341495086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120410.1778123396831482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126300.058.293871
GTATCAA229900.040.665841
ATCAACG281700.032.7721863
TCAACGC283700.032.557724
GTGGTAT51700.031.5957321
CAACGCA294500.031.3956745
TATCAAC298600.031.0432992
AACGCAG307350.030.1701376
TGGTATC55650.027.8740182
ACGCAGA346550.026.581147
CGCAGAG349550.026.2723378
GCAGAGT386650.023.65429
GTACATG174150.022.2467141
GAGTACT283600.021.94144212-13
CAGAGTA375650.021.37543710-11
TACATGG177800.020.885562
AGAGTAC359700.020.585510-11
GTACTTT313400.019.70515314-15
ACATGGG182600.019.5127833
AGTACTT301200.019.21599612-13
Produced by FastQC (version 0.11.5)