##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049476_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6771746 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.948404443994207 33.0 31.0 34.0 30.0 34.0 2 32.1765212398693 33.0 31.0 34.0 30.0 34.0 3 32.18046305930553 34.0 31.0 34.0 30.0 34.0 4 35.85789765298344 37.0 35.0 37.0 35.0 37.0 5 35.90694142987643 37.0 35.0 37.0 35.0 37.0 6 35.965441704399424 37.0 35.0 37.0 35.0 37.0 7 35.935714806786905 37.0 35.0 37.0 35.0 37.0 8 35.94657936077343 37.0 35.0 37.0 35.0 37.0 9 37.68117838442257 39.0 37.0 39.0 35.0 39.0 10-11 37.6547568381921 39.0 37.0 39.0 35.0 39.0 12-13 37.631408354654766 39.0 37.0 39.0 35.0 39.0 14-15 38.9386291216475 40.0 38.0 41.0 36.0 41.0 16-17 38.949021493127475 40.0 38.0 41.0 36.0 41.0 18-19 39.027706886820624 40.0 38.5 41.0 36.0 41.0 20-21 39.05386225354584 40.0 39.0 41.0 36.0 41.0 22-23 39.02194278993926 40.0 39.0 41.0 35.0 41.0 24-25 39.00142193756234 40.0 39.0 41.0 35.0 41.0 26-27 38.90258487249817 40.0 38.0 41.0 35.0 41.0 28-29 38.82383922846486 40.0 38.0 41.0 35.0 41.0 30-31 38.71288999912282 40.0 38.0 41.0 35.0 41.0 32-33 38.57486976032474 40.0 38.0 41.0 35.0 41.0 34-35 38.460567038397485 40.0 38.0 41.0 34.0 41.0 36-37 38.32816115666476 40.0 38.0 41.0 34.0 41.0 38-39 38.22284703236063 40.0 38.0 41.0 34.0 41.0 40-41 38.129919816838964 40.0 38.0 41.0 34.0 41.0 42-43 37.98935946504786 40.0 38.0 41.0 33.0 41.0 44-45 37.8137161376106 40.0 38.0 41.0 33.0 41.0 46-47 37.56371628528299 40.0 37.5 41.0 33.0 41.0 48-49 37.31583479356728 40.0 37.0 41.0 32.5 41.0 50-51 37.16007570278035 40.0 37.0 41.0 32.0 41.0 52-53 37.04797743447554 40.0 37.0 41.0 32.0 41.0 54-55 36.909338891328765 40.0 36.5 41.0 31.0 41.0 56-57 36.76609156634049 40.0 36.0 41.0 31.0 41.0 58-59 36.596048419417976 39.0 36.0 41.0 31.0 41.0 60-61 36.414263249094105 39.0 35.0 41.0 31.0 41.0 62-63 36.201380559755194 39.0 35.0 41.0 31.0 41.0 64-65 35.986205994731634 39.0 35.0 41.0 30.5 41.0 66-67 35.72927358763899 38.0 35.0 40.0 30.0 41.0 68-69 35.44290253946323 37.5 35.0 40.0 30.0 41.0 70-71 35.15743923354479 37.0 35.0 40.0 29.5 41.0 72-73 34.82698199253191 36.5 34.5 39.0 29.0 41.0 74-75 34.501100971595804 36.0 34.0 39.0 29.0 41.0 76-77 34.179179343111805 36.0 34.0 39.0 29.0 40.0 78-79 33.86548469183575 35.0 34.0 37.5 29.0 39.5 80-81 33.54755509140479 35.0 34.0 37.0 29.0 39.0 82-83 33.26457748119908 35.0 34.0 37.0 29.0 39.0 84-85 32.974102469289306 35.0 34.0 36.0 28.5 38.0 86-87 32.71953577408249 35.0 34.0 36.0 28.0 37.0 88-89 32.49027673217513 35.0 34.0 35.5 27.5 37.0 90-91 32.29149195790864 35.0 34.0 35.0 27.0 36.5 92-93 32.12268490283008 35.0 33.0 35.0 27.0 36.0 94-95 31.95706498737549 35.0 33.0 35.0 27.0 36.0 96-97 31.809884112605523 35.0 33.0 35.0 26.0 36.0 98-99 31.672475459061815 35.0 33.0 35.0 26.0 35.5 100 31.564302618556574 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3498254381546033 1101 2 0.34311909696739207 1101 3 0.4841741024567625 1101 4 0.2889719715397092 1101 5 0.27436275644471664 1101 6 0.27646677581146406 1101 7 0.25760987129732626 1101 8 0.28909683742026715 1101 9 0.34861945198197475 1101 10-11 0.3277049773205647 1101 12-13 0.31236788354379996 1101 14-15 0.4387381759102382 1101 16-17 0.4087172327427311 1101 18-19 0.4205688662303686 1101 20-21 0.4368718300750558 1101 22-23 0.41672629127072724 1101 24-25 0.404239572029077 1101 26-27 0.37775763783690053 1101 28-29 0.3907281404855354 1101 30-31 0.38941708817122844 1101 32-33 0.4036544954375145 1101 34-35 0.39432473306170834 1101 36-37 0.4981407961259805 1101 38-39 0.4379810685468968 1101 40-41 0.4214646787991114 1101 42-43 0.40411650813607025 1101 44-45 0.4493146980017926 1101 46-47 0.42076385991972387 1101 48-49 0.4551865139739988 1101 50-51 0.3971753351431744 1101 52-53 0.36331012838882515 1101 54-55 0.3667071604991392 1101 56-57 0.397977210743349 1101 58-59 0.3625303443505814 1101 60-61 0.2908412705092971 1101 62-63 0.337635432725925 1101 64-65 0.33036773578604794 1101 66-67 0.3776566182672667 1101 68-69 0.32976204300801726 1101 70-71 0.3342787715938442 1101 72-73 0.33675458098211664 1101 74-75 0.34985269024091536 1101 76-77 0.33798678559244166 1101 78-79 0.2985127363966029 1101 80-81 0.3161631254740982 1101 82-83 0.28453663910249105 1101 84-85 0.2904358383865997 1101 86-87 0.21114159749453876 1101 88-89 0.2761236828039628 1101 90-91 0.30880008497567957 1101 92-93 0.30023552280634647 1101 94-95 0.3038780926268174 1101 96-97 0.3093526765624013 1101 98-99 0.33980075279125543 1101 100 0.36053236825064516 1102 1 0.2627002442815254 1102 2 0.3122933041236067 1102 3 0.326312257346963 1102 4 0.2326708464807652 1102 5 0.21831426712391533 1102 6 0.17375517679441543 1102 7 0.2006211794337176 1102 8 0.16173072264997046 1102 9 0.23181574160383178 1102 10-11 0.23503516735547691 1102 12-13 0.2485260489726926 1102 14-15 0.2805856653461518 1102 16-17 0.31299252546453715 1102 18-19 0.3039932806446686 1102 20-21 0.31122085728597426 1102 22-23 0.2591129620627015 1102 24-25 0.26885105386205055 1102 26-27 0.23827379424191975 1102 28-29 0.24210438341876994 1102 30-31 0.2576071799659303 1102 32-33 0.320140775098281 1102 34-35 0.324888921646199 1102 36-37 0.35032336772714245 1102 38-39 0.28623209159203356 1102 40-41 0.2892596765021054 1102 42-43 0.2139038470573098 1102 44-45 0.2213465068957916 1102 46-47 0.24442988722107373 1102 48-49 0.2598532767582924 1102 50-51 0.2555607335774823 1102 52-53 0.20338831158782966 1102 54-55 0.24830827087887286 1102 56-57 0.2532135679557257 1102 58-59 0.25684290369733986 1102 60-61 0.20928148214785125 1102 62-63 0.17613966602723252 1102 64-65 0.17540491192915653 1102 66-67 0.1965175252433653 1102 68-69 0.23317401896453305 1102 70-71 0.2090087974678454 1102 72-73 0.2620029619339732 1102 74-75 0.2013005279641149 1102 76-77 0.19938512797003938 1102 78-79 0.15850592921414375 1102 80-81 0.18488660231827936 1102 82-83 0.14296752813987723 1102 84-85 0.11397293799468144 1102 86-87 0.10044736071640159 1102 88-89 0.0947347719386542 1102 90-91 0.11701323025708632 1102 92-93 0.11144989457433496 1102 94-95 0.0748783372963544 1102 96-97 0.11482541131881874 1102 98-99 0.15064245474286508 1102 100 0.1518699681887803 1103 1 0.27679688907767996 1103 2 0.3004504526149745 1103 3 0.36771212988703184 1103 4 0.1946878975423516 1103 5 0.2193594394842151 1103 6 0.22034339556056182 1103 7 0.21149323176393864 1103 8 0.18789295279449192 1103 9 0.22898717839046867 1103 10-11 0.24346915538230007 1103 12-13 0.2179728572525832 1103 14-15 0.3502395478043496 1103 16-17 0.343693697808682 1103 18-19 0.31774732780611714 1103 20-21 0.296539419881519 1103 22-23 0.2970947141782503 1103 24-25 0.30700352623603067 1103 26-27 0.29101144769815335 1103 28-29 0.31460013594548286 1103 30-31 0.3212266418439711 1103 32-33 0.3149261271299437 1103 34-35 0.30016245352629056 1103 36-37 0.27317753296678404 1103 38-39 0.26004770604418326 1103 40-41 0.22619095166616177 1103 42-43 0.2589837570480569 1103 44-45 0.24985563860885662 1103 46-47 0.23245368355363638 1103 48-49 0.24808623348455683 1103 50-51 0.22565755280347588 1103 52-53 0.22056827270277068 1103 54-55 0.2794611162305003 1103 56-57 0.27720646307068364 1103 58-59 0.2586900948516373 1103 60-61 0.2257545747224654 1103 62-63 0.29540558275599693 1103 64-65 0.2356581705374836 1103 66-67 0.2536713302767666 1103 68-69 0.23823976174898576 1103 70-71 0.2345207841082555 1103 72-73 0.24209790322578328 1103 74-75 0.25103980549194915 1103 76-77 0.21889955730890165 1103 78-79 0.2464503052890592 1103 80-81 0.15814345931351426 1103 82-83 0.16128632009433375 1103 84-85 0.18150332203732944 1103 86-87 0.13282984796932595 1103 88-89 0.17087705087588034 1103 90-91 0.1682838454741784 1103 92-93 0.1572071042069041 1103 94-95 0.1179273182986833 1103 96-97 0.1815489836038644 1103 98-99 0.2748886577003802 1103 100 0.22961513894046348 1104 1 0.2354021892817144 1104 2 0.2775280833996021 1104 3 0.32558246191988616 1104 4 0.2431944261179737 1104 5 0.17440077502538287 1104 6 0.1677025078408576 1104 7 0.18690541335877953 1104 8 0.2035585883154667 1104 9 0.23359500237686603 1104 10-11 0.24469174164609342 1104 12-13 0.2467468829117223 1104 14-15 0.3098227603654564 1104 16-17 0.3146826677597119 1104 18-19 0.2691125279204556 1104 20-21 0.3083525972385672 1104 22-23 0.2908072333580236 1104 24-25 0.29142236418825007 1104 26-27 0.2615871122797415 1104 28-29 0.2668445999358937 1104 30-31 0.30242022493216325 1104 32-33 0.2911238521924062 1104 34-35 0.29692002112017946 1104 36-37 0.3140923385807639 1104 38-39 0.30726431978864355 1104 40-41 0.28827202470402824 1104 42-43 0.30034662562926684 1104 44-45 0.3000618844284304 1104 46-47 0.2791128811624688 1104 48-49 0.2692860074502974 1104 50-51 0.2533823450378918 1104 52-53 0.3250309111027363 1104 54-55 0.2719593822669637 1104 56-57 0.27669954608027325 1104 58-59 0.289364507128397 1104 60-61 0.24130769135957308 1104 62-63 0.2794872883277435 1104 64-65 0.2819608607168078 1104 66-67 0.30342146285606475 1104 68-69 0.3406770493604796 1104 70-71 0.2728629835481655 1104 72-73 0.28874210992077565 1104 74-75 0.2530882192248143 1104 76-77 0.23408807272846133 1104 78-79 0.2194300751269651 1104 80-81 0.23552473987000866 1104 82-83 0.22423983603367503 1104 84-85 0.1868050251661444 1104 86-87 0.1735681023925011 1104 88-89 0.16123033253922614 1104 90-91 0.1660508955499722 1104 92-93 0.13124712764059865 1104 94-95 0.108553210491948 1104 96-97 0.11692126108311385 1104 98-99 0.1687442193533535 1104 100 0.11644931771927247 1105 1 0.21781609863575113 1105 2 0.16323156226516744 1105 3 0.27093299145547434 1105 4 0.16179638991032874 1105 5 0.18889560770717395 1105 6 0.11396280958686589 1105 7 0.14754366936833208 1105 8 0.08872686387131523 1105 9 0.1866719882527832 1105 10-11 0.1389630252647862 1105 12-13 0.16933020136605847 1105 14-15 0.224319810213224 1105 16-17 0.1992715435185417 1105 18-19 0.18725336523979763 1105 20-21 0.18979082141489556 1105 22-23 0.18558780440600486 1105 24-25 0.2133820129421764 1105 26-27 0.18747136505662354 1105 28-29 0.24488907716632724 1105 30-31 0.27901862540109335 1105 32-33 0.24444127576919072 1105 34-35 0.24845152484619604 1105 36-37 0.2603731968324965 1105 38-39 0.26531531930468333 1105 40-41 0.2598598503190743 1105 42-43 0.22596474507313502 1105 44-45 0.28349003709161735 1105 46-47 0.29044450811004907 1105 48-49 0.30599787102087816 1105 50-51 0.2021434925372887 1105 52-53 0.18111841192499867 1105 54-55 0.24868809629894884 1105 56-57 0.27244075200712814 1105 58-59 0.2632755849706001 1105 60-61 0.19133812967125863 1105 62-63 0.19980997848129078 1105 64-65 0.2086612801869876 1105 66-67 0.19432199344883827 1105 68-69 0.21206064609118158 1105 70-71 0.2148111177222347 1105 72-73 0.2044703476291545 1105 74-75 0.27812163822983393 1105 76-77 0.24033174426133996 1105 78-79 0.20438858622512868 1105 80-81 0.19907981720945855 1105 82-83 0.1571342977586312 1105 84-85 0.16560649571982822 1105 86-87 0.18939323465893665 1105 88-89 0.15773836954563336 1105 90-91 0.1568630184849198 1105 92-93 0.16193894509412843 1105 94-95 0.16816083672063797 1105 96-97 0.23119773962446644 1105 98-99 0.29147764434912204 1105 100 0.20548438946735104 1106 1 0.1158467051612817 1106 2 0.15566355350383532 1106 3 0.25430096844666394 1106 4 0.15880494395572953 1106 5 0.1183175493792632 1106 6 0.1355870062931004 1106 7 0.12780961836563165 1106 8 0.10495990365372876 1106 9 0.15437775479133364 1106 10-11 0.14090821983751312 1106 12-13 0.1328237608097993 1106 14-15 0.1985705402161173 1106 16-17 0.17878601894962998 1106 18-19 0.18475247574047415 1106 20-21 0.21901107597636127 1106 22-23 0.22457576242550914 1106 24-25 0.2042668645911263 1106 26-27 0.20083928070303614 1106 28-29 0.22186605802158965 1106 30-31 0.21117141878411871 1106 32-33 0.24709157209677102 1106 34-35 0.2283416967019889 1106 36-37 0.20982396732294717 1106 38-39 0.2010111773696508 1106 40-41 0.2028478674232872 1106 42-43 0.17993456763820603 1106 44-45 0.2277001471635245 1106 46-47 0.2202685002475917 1106 48-49 0.24255267709388306 1106 50-51 0.24363371224200137 1106 52-53 0.22518870432651283 1106 54-55 0.2529689126882104 1106 56-57 0.25520654118513164 1106 58-59 0.20566859805834525 1106 60-61 0.2330326032813801 1106 62-63 0.24799581958927064 1106 64-65 0.2145764910451433 1106 66-67 0.22829348200900768 1106 68-69 0.2636890998023489 1106 70-71 0.2544267700690881 1106 72-73 0.28914014965985757 1106 74-75 0.28610695293501465 1106 76-77 0.28245369413425436 1106 78-79 0.22859846544694307 1106 80-81 0.1618609700264031 1106 82-83 0.1982432112198751 1106 84-85 0.20981392599341575 1106 86-87 0.19142059895136754 1106 88-89 0.1529278086864494 1106 90-91 0.20738738721483685 1106 92-93 0.23610030543724747 1106 94-95 0.15855355793637216 1106 96-97 0.18313454771992 1106 98-99 0.20474883703372626 1106 100 0.1857766521469948 1107 1 0.1275118473410899 1107 2 0.12284684332135498 1107 3 0.23193422890151538 1107 4 0.11799474084239847 1107 5 0.13689980526324774 1107 6 0.08846011816978461 1107 7 0.0706181814578315 1107 8 0.07171401429000213 1107 9 0.08519110584045109 1107 10-11 0.12294669557685722 1107 12-13 0.10618048610205477 1107 14-15 0.14284747240504458 1107 16-17 0.1309794294042561 1107 18-19 0.1421000125239189 1107 20-21 0.1383346135980119 1107 22-23 0.18752885154562904 1107 24-25 0.1875459445314931 1107 26-27 0.18381911824504016 1107 28-29 0.17266115822970107 1107 30-31 0.13040270963492873 1107 32-33 0.10359214293502816 1107 34-35 0.1320208456644636 1107 36-37 0.10956675336234412 1107 38-39 0.1160437282273108 1107 40-41 0.19099430954506147 1107 42-43 0.14719532373342048 1107 44-45 0.22534272968668034 1107 46-47 0.15797173080097338 1107 48-49 0.11004820691511696 1107 50-51 0.04324064046537757 1107 52-53 0.07162445639452386 1107 54-55 0.11570955639552238 1107 56-57 0.08221473159095183 1107 58-59 0.0892380344293997 1107 60-61 0.16699137188084734 1107 62-63 0.13806178584808038 1107 64-65 0.10806890057713758 1107 66-67 0.11912775901620876 1107 68-69 0.026913777906671044 1107 70-71 0.09262327835013195 1107 72-73 0.03961028813526468 1107 74-75 0.030072466858705127 1107 76-77 0.04434516292197799 1107 78-79 0.06950191967813169 1107 80-81 0.07603190796596948 1107 82-83 0.11539587679811802 1107 84-85 0.08014772774713919 1107 86-87 0.04689530735300451 1107 88-89 0.08143055288061163 1107 90-91 0.09875508245067266 1107 92-93 0.10555321728962497 1107 94-95 0.06165637075208963 1107 96-97 0.05776166302398167 1107 98-99 0.10715389374330186 1107 100 0.11169308984610282 1108 1 0.05212981012723361 1108 2 0.07960460059354801 1108 3 0.142248816192307 1108 4 0.07428905835487853 1108 5 0.10217168168394153 1108 6 0.07902753596333412 1108 7 0.0484530293757075 1108 8 0.08090612616855708 1108 9 0.08831615134612036 1108 10-11 0.06386387078259759 1108 12-13 0.05545851402092694 1108 14-15 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2215 7 -0.2154898963306806 2215 8 -0.2123319119117255 2215 9 -0.3971288616326234 2215 10-11 -0.3475307199605737 2215 12-13 -0.38184197204876114 2215 14-15 -0.4974591002145843 2215 16-17 -0.5017884259237846 2215 18-19 -0.4755050619947383 2215 20-21 -0.48979820169434873 2215 22-23 -0.4649833615603782 2215 24-25 -0.4588063226193171 2215 26-27 -0.3945509324987597 2215 28-29 -0.3766254377305387 2215 30-31 -0.467397412605024 2215 32-33 -0.40469153076250564 2215 34-35 -0.46923313424471047 2215 36-37 -0.4874603115708709 2215 38-39 -0.45493570724229926 2215 40-41 -0.4910174892531174 2215 42-43 -0.4166745549650628 2215 44-45 -0.42109421138049896 2215 46-47 -0.45460777416649734 2215 48-49 -0.4094158512862265 2215 50-51 -0.3672379532228405 2215 52-53 -0.41159012035154774 2215 54-55 -0.37632980859027754 2215 56-57 -0.3833884830960059 2215 58-59 -0.3984861367635446 2215 60-61 -0.4027550473722883 2215 62-63 -0.43417230761496484 2215 64-65 -0.41191322802539077 2215 66-67 -0.36699329971388295 2215 68-69 -0.3668133751082081 2215 70-71 -0.340753721849957 2215 72-73 -0.3339804019888746 2215 74-75 -0.2828266047037076 2215 76-77 -0.3593835679999273 2215 78-79 -0.32560807152888316 2215 80-81 -0.4028169073894716 2215 82-83 -0.3845542526615304 2215 84-85 -0.3201382283061349 2215 86-87 -0.3167541106428118 2215 88-89 -0.3232526063901915 2215 90-91 -0.34329812551813177 2215 92-93 -0.299705798030395 2215 94-95 -0.3202875255788271 2215 96-97 -0.25435003061998884 2215 98-99 -0.26820233559740103 2215 100 -0.28741930610178557 2216 1 -0.26140354362819807 2216 2 -0.28859323697132666 2216 3 -0.20272666826158314 2216 4 -0.20069430846457692 2216 5 -0.23215243745209335 2216 6 -0.20573157312136914 2216 7 -0.21614198711384347 2216 8 -0.2410340769489281 2216 9 -0.34932932962848184 2216 10-11 -0.30270884009579646 2216 12-13 -0.36830051571426736 2216 14-15 -0.4766110979088012 2216 16-17 -0.5203721787001641 2216 18-19 -0.45644913385723385 2216 20-21 -0.459159318414315 2216 22-23 -0.386763115013089 2216 24-25 -0.44075590437040546 2216 26-27 -0.4636186222753622 2216 28-29 -0.4443557066734627 2216 30-31 -0.43543677145287063 2216 32-33 -0.46052350575713774 2216 34-35 -0.46286957862148626 2216 36-37 -0.48716268816151853 2216 38-39 -0.43625057096876674 2216 40-41 -0.5220873431441788 2216 42-43 -0.590371269006674 2216 44-45 -0.5742860020210188 2216 46-47 -0.5215415050140564 2216 48-49 -0.4856596505582331 2216 50-51 -0.4863283419112534 2216 52-53 -0.4605049556356775 2216 54-55 -0.46514269519437335 2216 56-57 -0.45827929016903113 2216 58-59 -0.46154821555642656 2216 60-61 -0.4717744964485604 2216 62-63 -0.5008702018235809 2216 64-65 -0.5440416904999594 2216 66-67 -0.4965968398620788 2216 68-69 -0.5195688214900613 2216 70-71 -0.5462564320138625 2216 72-73 -0.5018551487760874 2216 74-75 -0.4731578995790855 2216 76-77 -0.4001231447518947 2216 78-79 -0.3558285362788354 2216 80-81 -0.36216201647452095 2216 82-83 -0.4240916284162779 2216 84-85 -0.4725848358246907 2216 86-87 -0.3966083633421249 2216 88-89 -0.42554548144909177 2216 90-91 -0.40165316716661437 2216 92-93 -0.37048546952140526 2216 94-95 -0.4091724746974421 2216 96-97 -0.44153911896709985 2216 98-99 -0.48100582116779833 2216 100 -0.4878749669953102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 509.0 3 48.0 4 60.0 5 136.0 6 523.0 7 1295.0 8 2854.0 9 4679.0 10 5640.0 11 7516.0 12 10755.0 13 15362.0 14 18733.0 15 24148.0 16 30422.0 17 42826.0 18 44134.0 19 23931.0 20 16415.0 21 15744.0 22 16135.0 23 17264.0 24 20079.0 25 24795.0 26 31407.0 27 40698.0 28 53457.0 29 70528.0 30 91256.0 31 119413.0 32 154716.0 33 204248.0 34 280667.0 35 413040.0 36 682296.0 37 1330826.0 38 2038703.0 39 913248.0 40 3240.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.10378109986841 20.949296344058926 15.140206982246054 22.80671557382661 2 15.322606606922351 21.07689804077117 40.22822179095317 23.372273561353303 3 17.54017353870036 26.000443607896695 31.472606917034398 24.986775936368552 4 12.468246741682277 18.116406019954084 38.83341164893072 30.581935589432916 5 12.318269924362951 37.81338450241007 35.980915572795716 13.887430000431266 6 29.406029782684456 38.93694659554737 17.818515827588854 13.838507794179321 7 25.370679880787023 33.42975356724839 23.181894300229217 18.017672251735373 8 24.075268032793907 36.37355860659866 20.9046381834168 18.64653517719064 9 24.273976017411165 16.93958987829727 22.404399101797377 36.38203500249419 10-11 23.046020922816655 27.413314084727926 29.34924021072261 20.191424781732806 12-13 24.026521372774464 25.163362595112105 29.546678212679566 21.263437819433864 14-15 21.330724749569757 27.130573119547012 27.48184884666377 24.05685328421946 16-17 20.082095518644675 30.29699430545682 28.19447894235844 21.426431233540065 18-19 19.913763747193116 28.48740930330228 31.34721089656936 20.25161605293524 20-21 20.96973217837763 27.74548838659926 31.214069458600484 20.070709976422624 22-23 20.678130869055042 27.627690111235715 31.250086757536387 20.44409226217286 24-25 20.559764054942402 27.70461266562567 31.231022250391554 20.504601029040366 26-27 20.363603419265875 27.666889750442504 31.285557668583554 20.683949161708075 28-29 20.33985031334607 27.632467313452096 31.49509742391401 20.53258494928782 30-31 20.678330226798227 27.970238399372928 31.02954540823002 20.321885965598828 32-33 20.350202148751592 27.794515624183187 31.30701446864664 20.548267758418582 34-35 20.563610921023916 27.570068339834364 31.23351053037134 20.632810208770383 36-37 20.832234404539097 27.815507256178833 30.78562751762987 20.5666308216522 38-39 20.696250272824763 27.927354333727223 30.86508265372033 20.511312739727686 40-41 20.62607634722271 27.74891438633404 30.876586333864264 20.748422932578983 42-43 20.6190397572502 27.78790728417752 30.66571014329244 20.92734281527984 44-45 20.821225426943066 27.937831690674752 30.425698187734746 20.815244694647436 46-47 20.923702690561637 28.114233758915354 29.99668032439492 20.965383226128093 48-49 20.954477619213716 28.07098051226375 29.805577468499262 21.168964400023274 50-51 20.98694339687283 28.12344851682269 29.57396807263592 21.315640013668556 52-53 20.99289459468799 28.223304595299354 29.50329206086584 21.280508749146822 54-55 21.082701566183964 28.499090190329053 29.30660718816093 21.111601055326055 56-57 20.946702667229395 28.2532082567775 29.373067152843596 21.427021923149507 58-59 20.824548055996196 27.931858341999245 29.679834417888678 21.56375918411588 60-61 21.028786372081882 28.20703109655914 29.400187189537235 21.36399534182174 62-63 20.849836954900553 28.18522726635051 29.50344711688832 21.46148866186062 64-65 20.867757000927085 28.001855060718462 29.57744575771153 21.552942180642926 66-67 20.80136348882548 28.035745877060364 29.44558906964319 21.717301564470965 68-69 21.14593488887504 28.187508804966992 29.293560331412312 21.372995974745656 70-71 21.142981440827814 28.01294525813579 29.307803334620054 21.53626996641634 72-73 20.958723200781602 28.157154742661643 29.344736202450594 21.539385854106165 74-75 21.041427129724003 28.141575304212534 29.182038133149117 21.634959432914346 76-77 21.067365787198753 28.17338393968114 29.011491275662145 21.747758997457968 78-79 21.165833745093217 28.217382931964668 29.07285654246335 21.543926780478774 80-81 20.966491361921246 28.07966470613904 29.13603411476075 21.81780981717896 82-83 20.865650136564746 28.12645250713761 29.228569236675035 21.77932811962261 84-85 20.888381517307497 28.04282782554917 29.086403319452565 21.98238733769077 86-87 20.99204668837217 28.164004425767057 28.998055512586745 21.845893373274023 88-89 20.837514024343196 28.057340043113204 29.00063241065861 22.10451352188499 90-91 20.757355600929074 27.94486342301228 29.009360804649532 22.288420171409115 92-93 20.793934548035327 28.120652190847256 28.897853938199987 22.187559322917433 94-95 20.629745052386582 27.942217672913305 29.048922471668774 22.379114803031342 96-97 20.632810310856325 28.00943928756416 28.972440071341047 22.385310330238468 98-99 20.597280783312087 28.03201314419988 28.858709007073013 22.511997065415017 100 20.68277567024492 27.955056166501347 28.821583696127778 22.540584467125957 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1340.0 1 1003.5 2 1183.5 3 1684.5 4 1108.5 5 745.5 6 1788.0 7 4711.5 8 8000.0 9 9255.5 10 7244.0 11 4584.0 12 4053.5 13 4455.5 14 5061.0 15 5677.5 16 6406.5 17 7335.5 18 8400.0 19 9964.5 20 12056.5 21 14722.5 22 18850.5 23 25188.0 24 34073.0 25 45666.0 26 60199.5 27 76974.0 28 96404.0 29 118478.5 30 139220.5 31 157003.5 32 173803.0 33 189209.0 34 202616.5 35 213739.5 36 223448.0 37 232501.5 38 239875.5 39 243297.0 40 245079.5 41 244489.5 42 240437.0 43 237018.0 44 232364.0 45 226886.0 46 221411.0 47 214312.0 48 207823.0 49 203493.5 50 197395.0 51 187473.5 52 174121.5 53 162270.5 54 154129.0 55 146029.0 56 134594.5 57 120294.5 58 108086.0 59 97518.5 60 83866.5 61 69196.0 62 57227.5 63 46169.0 64 36490.5 65 28790.5 66 22342.5 67 17170.0 68 12957.5 69 9534.5 70 6944.0 71 5003.0 72 3512.0 73 2346.0 74 1628.5 75 1068.0 76 613.5 77 398.0 78 261.0 79 146.5 80 84.0 81 44.0 82 28.0 83 15.5 84 7.5 85 4.0 86 1.5 87 0.5 88 1.0 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13492827403744914 2 0.0 3 0.0 4 0.0 5 0.014619567833761043 6 3.839482461391788E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1412498342377283E-4 82-83 7.309783916880521E-4 84-85 3.9871548637530115E-4 86-87 5.685387490907072E-4 88-89 5.685387490907072E-4 90-91 7.752801123964189E-4 92-93 2.4365946389601735E-4 94-95 3.174956650766287E-4 96-97 7.162111514519299E-4 98-99 0.0013290516212510037 100 7.383620118061133E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6771746.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.99637292239635 #Duplication Level Percentage of deduplicated Percentage of total 1 75.07688626341442 45.79417752375562 2 15.241242930888928 18.593210752266764 3 4.667536801964629 8.541084462049312 4 1.9013881048924393 4.639111116649107 5 0.9302830332052533 2.8371945408382824 6 0.5295312579275896 1.9379691649570145 7 0.35100029699956603 1.4986821508160184 8 0.2408185022216226 1.1751244134498422 9 0.18458588449478522 1.0133162502168866 >10 0.8153234496057806 8.649631649086425 >50 0.0417190109309029 1.7361440909654005 >100 0.018352458696196935 1.9439955837694718 >500 8.476743361642831E-4 0.3365045389436465 >1k 3.874643391204877E-4 0.38899953745165433 >5k 2.4216520638782663E-5 0.09382665755762236 >10k+ 7.264956191634798E-5 0.8210275672269085 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21795 0.32185200094628474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21747 0.32114317341495086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12041 0.1778123396831482 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.4767240236122265E-5 0.0 0.0 0.0 5 0.0 1.4767240236122265E-5 0.0 0.0 0.0 6 0.0 1.4767240236122265E-5 0.0 0.0 0.0 7 0.0 1.4767240236122265E-5 0.0 1.4767240236122265E-5 0.0 8 0.0 1.4767240236122265E-5 0.0 1.4767240236122265E-5 0.0 9 0.0 1.4767240236122265E-5 0.0 2.953448047224453E-5 0.0 10-11 0.0 1.4767240236122265E-5 0.0 5.906896094448906E-5 0.0 12-13 0.0 2.2150860354183397E-5 0.0 5.906896094448906E-5 0.0 14-15 1.4767240236122265E-5 2.953448047224453E-5 0.0 9.598706153479472E-5 0.0 16-17 1.4767240236122265E-5 2.953448047224453E-5 0.0 1.3290516212510038E-4 0.0 18-19 1.4767240236122265E-5 5.906896094448906E-5 0.0 1.4767240236122263E-4 0.0 20-21 1.4767240236122265E-5 7.383620118061132E-5 0.0 1.845905029515283E-4 0.0 22-23 1.4767240236122265E-5 7.383620118061132E-5 0.0 2.731939443682619E-4 0.0 24-25 1.4767240236122265E-5 7.383620118061132E-5 0.0 4.873189277920347E-4 0.0 26-27 1.4767240236122265E-5 7.383620118061132E-5 0.0 0.001114926637827231 0.0 28-29 1.4767240236122265E-5 7.383620118061132E-5 0.0 0.0028796118460438416 0.0 30-31 1.4767240236122265E-5 7.383620118061132E-5 0.0 0.009104003605569375 0.0 32-33 1.4767240236122265E-5 7.383620118061132E-5 0.0 0.024491467931608776 0.0 34-35 2.953448047224453E-5 7.383620118061132E-5 0.0 0.05127924171993456 0.0 36-37 2.953448047224453E-5 7.383620118061132E-5 0.0 0.09233216957635446 0.0 38-39 2.953448047224453E-5 7.383620118061132E-5 0.0 0.1423857303566909 0.0 40-41 2.953448047224453E-5 7.383620118061132E-5 0.0 0.19486111853575133 0.0 42-43 2.953448047224453E-5 7.383620118061132E-5 0.0 0.25093971333242565 0.0 44-45 2.953448047224453E-5 7.383620118061132E-5 0.0 0.32320320342788994 0.0 46-47 2.953448047224453E-5 7.383620118061132E-5 0.0 0.41425800672381985 0.0 48-49 2.953448047224453E-5 7.383620118061132E-5 0.0 0.5084508485699257 0.0 50-51 2.953448047224453E-5 1.4767240236122263E-4 0.0 0.6019939318456422 0.0 52-53 2.953448047224453E-5 1.9197412306958943E-4 0.0 0.6963270624739912 0.0 54-55 2.953448047224453E-5 2.2150860354183398E-4 0.0 0.8083661141454508 0.0 56-57 2.953448047224453E-5 2.2150860354183398E-4 0.0 0.9462699870904786 0.0 58-59 2.953448047224453E-5 2.2150860354183398E-4 0.0 1.0990813890538718 0.0 60-61 2.953448047224453E-5 2.2150860354183398E-4 0.0 1.2555993683165316 0.0 62-63 2.953448047224453E-5 2.2150860354183398E-4 0.0 1.4140592396702416 0.0 64-65 2.953448047224453E-5 2.3627584377795623E-4 0.0 1.5876112305452685 0.0 66-67 2.953448047224453E-5 2.3627584377795623E-4 0.0 1.7919750681729645 0.0 68-69 2.953448047224453E-5 2.3627584377795623E-4 0.0 2.022129890873048 0.0 70-71 2.953448047224453E-5 2.4365946389601738E-4 0.0 2.263928682499314 0.0 72-73 2.953448047224453E-5 2.6581032425020075E-4 0.0 2.513930676076746 0.0 74-75 2.953448047224453E-5 2.6581032425020075E-4 0.0 2.775923668725909 0.0 76-77 2.953448047224453E-5 2.6581032425020075E-4 0.0 3.06081326736118 0.0 78-79 2.953448047224453E-5 2.6581032425020075E-4 0.0 3.3817275485524707 0.0 80-81 2.953448047224453E-5 2.6581032425020075E-4 0.0 3.7310687671982974 0.0 82-83 2.953448047224453E-5 2.6581032425020075E-4 0.0 4.091308209138382 0.0 84-85 2.953448047224453E-5 2.6581032425020075E-4 0.0 4.455054870634545 0.0 86-87 2.953448047224453E-5 2.6581032425020075E-4 0.0 4.845478551617264 0.0 88 4.4301720708366794E-5 2.6581032425020075E-4 0.0 5.163734729566053 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12630 0.0 58.29387 1 GTATCAA 22990 0.0 40.66584 1 ATCAACG 28170 0.0 32.772186 3 TCAACGC 28370 0.0 32.55772 4 GTGGTAT 5170 0.0 31.595732 1 CAACGCA 29450 0.0 31.395674 5 TATCAAC 29860 0.0 31.043299 2 AACGCAG 30735 0.0 30.170137 6 TGGTATC 5565 0.0 27.874018 2 ACGCAGA 34655 0.0 26.58114 7 CGCAGAG 34955 0.0 26.272337 8 GCAGAGT 38665 0.0 23.6542 9 GTACATG 17415 0.0 22.246714 1 GAGTACT 28360 0.0 21.941442 12-13 CAGAGTA 37565 0.0 21.375437 10-11 TACATGG 17780 0.0 20.88556 2 AGAGTAC 35970 0.0 20.5855 10-11 GTACTTT 31340 0.0 19.705153 14-15 ACATGGG 18260 0.0 19.512783 3 AGTACTT 30120 0.0 19.215996 12-13 >>END_MODULE