FastQCFastQC Report
Wed 25 May 2016
SRR2049475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6234298
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66250.047.7730831
GTATCAA113800.037.1510851
ATCAACG126550.032.607283
TCAACGC125950.032.5755164
CAACGCA138600.029.7726155
TATCAAC140700.029.6615682
AACGCAG150550.027.4410536
ACGCAGA167900.024.1579327
CGCAGAG166700.024.076738
GTACATG197950.022.3794541
TACATGG201100.021.2435472
ACATGGG207400.019.9867443
GCAGAGT192300.019.8697999
GTGGTAT36250.019.0705571
CAGAGTA179150.017.8128510-11
CATGGGG141350.017.5887554
AGAGTAC166600.017.33513810-11
GAGTACA153700.016.4000421
GTCTAGG16100.014.6048861
AGTACAT163300.014.5050622
Produced by FastQC (version 0.11.5)