##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049475_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6234298 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.553346984696592 31.0 31.0 34.0 27.0 34.0 2 30.795413693731035 31.0 31.0 34.0 27.0 34.0 3 30.857121844352 31.0 31.0 34.0 27.0 34.0 4 34.458045156006335 37.0 35.0 37.0 31.0 37.0 5 34.47701425244671 37.0 35.0 37.0 31.0 37.0 6 34.46636894803553 37.0 35.0 37.0 31.0 37.0 7 34.38154063216099 37.0 35.0 37.0 30.0 37.0 8 34.338700844906676 37.0 35.0 37.0 30.0 37.0 9 35.84601393773605 39.0 37.0 39.0 30.0 39.0 10-11 35.7838747040966 39.0 37.0 39.0 30.0 39.0 12-13 35.711165154440806 39.0 37.0 39.0 30.0 39.0 14-15 36.878083386453454 40.0 37.0 41.0 30.0 41.0 16-17 36.856511350596335 40.0 37.0 41.0 30.0 41.0 18-19 36.86104730636873 40.0 37.0 41.0 30.0 41.0 20-21 36.920639180225265 40.0 37.0 41.0 30.0 41.0 22-23 36.86551003817912 40.0 37.0 41.0 30.0 41.0 24-25 36.86326648164717 40.0 37.0 41.0 30.0 41.0 26-27 36.8616863518555 40.0 37.0 41.0 30.0 41.0 28-29 36.83607424604983 40.0 37.0 41.0 30.0 41.0 30-31 36.7751758898917 40.0 37.0 41.0 30.0 41.0 32-33 36.731591511987396 40.0 37.0 41.0 30.0 41.0 34-35 36.6554537495641 40.0 37.0 41.0 30.0 41.0 36-37 36.531653363377885 40.0 36.5 41.0 30.0 41.0 38-39 36.415212586886284 40.0 36.0 41.0 30.0 41.0 40-41 36.28681432937598 40.0 36.0 41.0 30.0 41.0 42-43 36.17625160042077 40.0 36.0 41.0 29.0 41.0 44-45 36.00371637672758 40.0 35.5 41.0 28.5 41.0 46-47 35.87792675293994 39.0 35.0 41.0 28.0 41.0 48-49 35.77282005127121 39.0 35.0 41.0 28.0 41.0 50-51 35.65402391736808 39.0 35.0 41.0 28.0 41.0 52-53 35.53250526362391 39.0 35.0 41.0 27.5 41.0 54-55 35.38033359970922 39.0 35.0 41.0 27.0 41.0 56-57 35.207960784036956 39.0 35.0 40.0 27.0 41.0 58-59 35.01689652948896 38.5 34.0 40.0 26.5 41.0 60-61 34.81583564019557 38.0 34.0 40.0 26.0 41.0 62-63 34.553026900542775 38.0 34.0 40.0 26.0 41.0 64-65 34.31065269898872 37.5 34.0 40.0 26.0 41.0 66-67 34.083480369401656 37.0 34.0 40.0 25.5 41.0 68-69 33.7921093120669 36.5 33.0 40.0 25.0 41.0 70-71 33.47650513337669 36.0 33.0 39.0 24.5 41.0 72-73 33.13874753821521 36.0 33.0 39.0 24.0 41.0 74-75 32.837504559454814 35.0 33.0 39.0 23.0 40.0 76-77 32.50821576061972 35.0 32.5 37.5 22.5 40.0 78-79 32.15856596845387 35.0 32.0 37.0 22.0 39.0 80-81 31.821410927100374 35.0 32.0 37.0 20.5 39.0 82-83 31.514892615014553 35.0 32.0 36.0 20.0 38.5 84-85 31.217718739142725 35.0 32.0 36.0 19.5 37.0 86-87 30.93593705337794 35.0 32.0 35.5 18.0 37.0 88-89 30.689681500627657 35.0 31.5 35.0 17.0 36.5 90-91 30.457949475626606 35.0 31.0 35.0 10.0 36.0 92-93 30.22392657521344 35.0 31.0 35.0 4.5 36.0 94-95 29.987839769609984 34.0 31.0 35.0 2.0 36.0 96-97 29.76369512654031 34.0 31.0 35.0 2.0 35.5 98-99 29.547754374269566 34.0 31.0 35.0 2.0 35.0 100 29.376814358248517 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3627979862326036 1101 2 0.3874916257831309 1101 3 0.40444509742508217 1101 4 0.4365552790488749 1101 5 0.41911197818917145 1101 6 0.44768830976525464 1101 7 0.5372468058941848 1101 8 0.5006086089722217 1101 9 0.6061851606875166 1101 10-11 0.6123311950240549 1101 12-13 0.5775836733988129 1101 14-15 0.702240455986761 1101 16-17 0.6151578725640761 1101 18-19 0.6912310134550594 1101 20-21 0.6445559682361051 1101 22-23 0.5933924518604243 1101 24-25 0.6682663046842379 1101 26-27 0.6623758989355082 1101 28-29 0.6132520379941866 1101 30-31 0.6276892750743102 1101 32-33 0.5972278142425225 1101 34-35 0.5895746233118615 1101 36-37 0.624409421711583 1101 38-39 0.5599132742064228 1101 40-41 0.6134173416694111 1101 42-43 0.5005322093701352 1101 44-45 0.5075491137727113 1101 46-47 0.4733178302879537 1101 48-49 0.423765450423474 1101 50-51 0.461743428471145 1101 52-53 0.41650328705584627 1101 54-55 0.42302714534566377 1101 56-57 0.40523468018007947 1101 58-59 0.42344546603214184 1101 60-61 0.4185503723773749 1101 62-63 0.42280761205721973 1101 64-65 0.45403477719431606 1101 66-67 0.42842743613848455 1101 68-69 0.45458261408318634 1101 70-71 0.492764547855721 1101 72-73 0.4830873653458525 1101 74-75 0.4484619295076939 1101 76-77 0.4266321420328225 1101 78-79 0.4271992564130258 1101 80-81 0.44055899547521093 1101 82-83 0.4077777293874121 1101 84-85 0.37056349146292433 1101 86-87 0.32715132206647013 1101 88-89 0.33493932515549574 1101 90-91 0.3641276601221435 1101 92-93 0.37480035418348834 1101 94-95 0.42539311573398564 1101 96-97 0.41879042077003703 1101 98-99 0.5073573597544829 1101 100 0.47251167410288275 1102 1 0.25445481150251936 1102 2 0.3289795632438448 1102 3 0.38023364095425904 1102 4 0.3690641426469057 1102 5 0.3258931221058816 1102 6 0.29555864372080265 1102 7 0.3377117387934945 1102 8 0.39132473245626187 1102 9 0.5166291360265589 1102 10-11 0.5109489302801791 1102 12-13 0.4583776257624592 1102 14-15 0.6220614353317373 1102 16-17 0.5152616739946794 1102 18-19 0.5254379847571613 1102 20-21 0.5417987731194245 1102 22-23 0.5682679683488132 1102 24-25 0.561538312060037 1102 26-27 0.4855155649475549 1102 28-29 0.5253311271737999 1102 30-31 0.47625497927328553 1102 32-33 0.5033826550049412 1102 34-35 0.5243499625776025 1102 36-37 0.5996777251243017 1102 38-39 0.5677868448671433 1102 40-41 0.5549677445807291 1102 42-43 0.541722622184615 1102 44-45 0.46080577885602736 1102 46-47 0.40064346354149905 1102 48-49 0.4031433689360142 1102 50-51 0.47609977294168004 1102 52-53 0.38318386624711565 1102 54-55 0.46785403939239245 1102 56-57 0.49585405478438815 1102 58-59 0.5326787558195534 1102 60-61 0.5068652819490254 1102 62-63 0.44411152937229303 1102 64-65 0.48576985407416373 1102 66-67 0.5035671731692233 1102 68-69 0.4820592959263408 1102 70-71 0.43271656514595236 1102 72-73 0.45874186813537676 1102 74-75 0.4483728602288153 1102 76-77 0.4576948522442805 1102 78-79 0.5398596561496376 1102 80-81 0.5142855098114829 1102 82-83 0.4902884318623464 1102 84-85 0.4837893775514779 1102 86-87 0.48384299511049633 1102 88-89 0.49448213808479125 1102 90-91 0.4723937006594454 1102 92-93 0.4113300876956991 1102 94-95 0.41289112714009235 1102 96-97 0.5132616224782041 1102 98-99 0.5112543696842771 1102 100 0.4373150488435904 1103 1 0.3629504951823179 1103 2 0.40427420677751513 1103 3 0.4044504834541627 1103 4 0.48461499335485314 1103 5 0.46378960625921906 1103 6 0.45773821839725315 1103 7 0.49829690692710926 1103 8 0.48209617416799944 1103 9 0.6106116741283714 1103 10-11 0.5812379959656937 1103 12-13 0.5439241742946166 1103 14-15 0.6855535314129142 1103 16-17 0.6596170060276236 1103 18-19 0.6677753736474372 1103 20-21 0.6335660819202147 1103 22-23 0.6358551298751465 1103 24-25 0.6710380150942115 1103 26-27 0.6588756872890471 1103 28-29 0.6330918229425606 1103 30-31 0.6611121765298122 1103 32-33 0.704039952295588 1103 34-35 0.6958513751130511 1103 36-37 0.6223659677185935 1103 38-39 0.6799709838758972 1103 40-41 0.7491517486323858 1103 42-43 0.6857794636037937 1103 44-45 0.6566651235891072 1103 46-47 0.6029018884796926 1103 48-49 0.6520966053619333 1103 50-51 0.6237840447340588 1103 52-53 0.6111518074470155 1103 54-55 0.5382083633264969 1103 56-57 0.5256463512481062 1103 58-59 0.5555679828517128 1103 60-61 0.5772002317136113 1103 62-63 0.656967511346096 1103 64-65 0.6558550061239501 1103 66-67 0.5906780939344856 1103 68-69 0.5625726331728487 1103 70-71 0.5431649270321657 1103 72-73 0.5933977632898859 1103 74-75 0.5800880747835322 1103 76-77 0.4695389702310351 1103 78-79 0.5370839268412269 1103 80-81 0.5857871831659232 1103 82-83 0.5041445209056832 1103 84-85 0.48686369778864247 1103 86-87 0.47895048563791676 1103 88-89 0.4852029876003243 1103 90-91 0.4589579916373978 1103 92-93 0.39089062642468164 1103 94-95 0.4157690774292 1103 96-97 0.36373960671390293 1103 98-99 0.34846864498969055 1103 100 0.31782106978307567 1104 1 0.23851133590622808 1104 2 0.25643839485544717 1104 3 0.29749348399742814 1104 4 0.3431159552326264 1104 5 0.37651850645805496 1104 6 0.3868414242949214 1104 7 0.4411168978912201 1104 8 0.40629291977028004 1104 9 0.5186638182782772 1104 10-11 0.5211640712033727 1104 12-13 0.5458642336202217 1104 14-15 0.5901009510222792 1104 16-17 0.5859598712362342 1104 18-19 0.5287257968986125 1104 20-21 0.5096049139954033 1104 22-23 0.599025903580575 1104 24-25 0.5896549184747073 1104 26-27 0.5472264046879616 1104 28-29 0.4961538802420904 1104 30-31 0.5080822742597348 1104 32-33 0.5399038414333219 1104 34-35 0.5499555142916535 1104 36-37 0.5672537361667267 1104 38-39 0.5323372342780459 1104 40-41 0.5664293671398397 1104 42-43 0.552393043180011 1104 44-45 0.5124591824321953 1104 46-47 0.4905935924041813 1104 48-49 0.5171972894403041 1104 50-51 0.5109243553350495 1104 52-53 0.49223926282205355 1104 54-55 0.4125684929336302 1104 56-57 0.384260166270856 1104 58-59 0.4150828659705681 1104 60-61 0.46105750064280215 1104 62-63 0.408059163556878 1104 64-65 0.4403184798510793 1104 66-67 0.3884236513517436 1104 68-69 0.3333311438164088 1104 70-71 0.37269996825516927 1104 72-73 0.35375205684817246 1104 74-75 0.33601530632189736 1104 76-77 0.3233862152392035 1104 78-79 0.2607309190461322 1104 80-81 0.31061841083242925 1104 82-83 0.33694888469812767 1104 84-85 0.2791431319285742 1104 86-87 0.26272475749926727 1104 88-89 0.24612211983902554 1104 90-91 0.27988746330464664 1104 92-93 0.24043716521038405 1104 94-95 0.29597081991689933 1104 96-97 0.33999797753083527 1104 98-99 0.334718596670438 1104 100 0.32265254929538045 1105 1 0.23786370225730025 1105 2 0.2714068723941594 1105 3 0.2958158657407921 1105 4 0.2747501987868972 1105 5 0.24415020604957505 1105 6 0.24298466397821983 1105 7 0.30899305056907167 1105 8 0.28411992440840805 1105 9 0.3295961081139609 1105 10-11 0.34392435822744716 1105 12-13 0.4072281629559882 1105 14-15 0.4501331612535111 1105 16-17 0.37950821094978693 1105 18-19 0.3867032698785877 1105 20-21 0.44045026086823924 1105 22-23 0.3891364989515651 1105 24-25 0.39528054741574437 1105 26-27 0.4669288714829207 1105 28-29 0.40405676948499547 1105 30-31 0.39843423266498235 1105 32-33 0.4151899782904991 1105 34-35 0.38268880929327054 1105 36-37 0.3737298663250286 1105 38-39 0.42132212711661765 1105 40-41 0.4215132200506062 1105 42-43 0.38402642210921556 1105 44-45 0.36285927294272824 1105 46-47 0.3637860101925767 1105 48-49 0.2656207488012541 1105 50-51 0.2828881163440329 1105 52-53 0.31347026882706785 1105 54-55 0.2999785399947754 1105 56-57 0.2561289953779138 1105 58-59 0.22786653422492975 1105 60-61 0.22348970372122068 1105 62-63 0.19709988366379605 1105 64-65 0.27164300095196126 1105 66-67 0.19223039900364824 1105 68-69 0.14979226879972884 1105 70-71 0.18803875095347422 1105 72-73 0.1737787776927746 1105 74-75 0.2101479547411813 1105 76-77 0.18964345573721886 1105 78-79 0.16294845635420785 1105 80-81 0.13004303822324204 1105 82-83 0.10587221131947899 1105 84-85 0.05152960125374051 1105 86-87 0.06840518696639464 1105 88-89 0.04171023780768479 1105 90-91 0.14228651095944045 1105 92-93 0.11367893049179756 1105 94-95 0.13284623392983974 1105 96-97 0.1388528553229591 1105 98-99 0.11987172176930372 1105 100 0.0834214053433655 1106 1 0.36760713315799975 1106 2 0.3626642103096671 1106 3 0.3836129552873757 1106 4 0.41490927614731987 1106 5 0.3875155574989222 1106 6 0.38127002486173467 1106 7 0.39883431188799534 1106 8 0.378930377415287 1106 9 0.4570855481060221 1106 10-11 0.440069153841506 1106 12-13 0.45672250905424505 1106 14-15 0.5559481916926003 1106 16-17 0.5517584012279215 1106 18-19 0.5647818686649018 1106 20-21 0.5038039788464204 1106 22-23 0.5233151731163375 1106 24-25 0.5426869282943514 1106 26-27 0.536050689793619 1106 28-29 0.4508381930214611 1106 30-31 0.48989581160688545 1106 32-33 0.4655908024791344 1106 34-35 0.48904083556577405 1106 36-37 0.4962507781306229 1106 38-39 0.5599002624383189 1106 40-41 0.630228571708983 1106 42-43 0.5352670313318271 1106 44-45 0.5170172688779147 1106 46-47 0.49700370519731507 1106 48-49 0.5318952954794156 1106 50-51 0.5344371692091556 1106 52-53 0.5101043034333301 1106 54-55 0.4980154398846892 1106 56-57 0.5025144036436515 1106 58-59 0.5536705759049454 1106 60-61 0.5578184302168907 1106 62-63 0.6004425771829887 1106 64-65 0.5558751157583401 1106 66-67 0.6557398953893312 1106 68-69 0.5982850940351199 1106 70-71 0.5905660246227811 1106 72-73 0.5923269173449626 1106 74-75 0.5743913859472016 1106 76-77 0.5190976716210187 1106 78-79 0.5434393833411875 1106 80-81 0.5314268287196775 1106 82-83 0.5399666607219871 1106 84-85 0.5770864222769241 1106 86-87 0.6048867362463781 1106 88-89 0.5787201104280797 1106 90-91 0.5638911730004352 1106 92-93 0.48332464371080874 1106 94-95 0.5651765268057396 1106 96-97 0.5997536987002974 1106 98-99 0.5780170962854818 1106 100 0.5874994969092029 1107 1 0.12278684486571834 1107 2 0.163243052892593 1107 3 0.20673560231415422 1107 4 0.150167366060451 1107 5 0.1351976190769406 1107 6 0.24414235394630168 1107 7 0.21178199569172307 1107 8 0.2212191695923309 1107 9 0.24613287552742236 1107 10-11 0.22290201309364477 1107 12-13 0.2250903967417699 1107 14-15 0.24128680974049388 1107 16-17 0.22329224775505452 1107 18-19 0.2817347781035622 1107 20-21 0.26578480261395754 1107 22-23 0.24298668142301239 1107 24-25 0.23527420680559175 1107 26-27 0.35437096851762107 1107 28-29 0.28427720056153305 1107 30-31 0.25483499412914057 1107 32-33 0.29343128859832035 1107 34-35 0.23292998239202234 1107 36-37 0.24751324386196671 1107 38-39 0.22264270437713662 1107 40-41 0.30970604274214253 1107 42-43 0.2686623813372577 1107 44-45 0.20289665271859292 1107 46-47 0.2289046489456581 1107 48-49 0.25396548021312526 1107 50-51 0.2592337947385417 1107 52-53 0.27831418474062275 1107 54-55 0.1958420513207173 1107 56-57 0.22635296563586138 1107 58-59 0.28483825992351086 1107 60-61 0.31862741047201837 1107 62-63 0.31173582073155615 1107 64-65 0.2592795267444927 1107 66-67 0.34027787518780883 1107 68-69 0.39654082478047314 1107 70-71 0.3493966839828957 1107 72-73 0.3235269168755792 1107 74-75 0.3432495915733327 1107 76-77 0.34942147621109854 1107 78-79 0.3168494782016609 1107 80-81 0.35759931425464586 1107 82-83 0.3312104048946978 1107 84-85 0.32181445226463623 1107 86-87 0.19309552013512743 1107 88-89 0.21243167403759955 1107 90-91 0.3103963812590109 1107 92-93 0.2465997071209749 1107 94-95 0.20000227037168372 1107 96-97 0.30308774672096916 1107 98-99 0.3534568174296915 1107 100 0.2541594386997552 1108 1 0.08615238866499197 1108 2 0.11157240435817428 1108 3 0.12794345045611166 1108 4 0.11591493100698358 1108 5 0.138180903571822 1108 6 0.11365994487417197 1108 7 0.0562715594849621 1108 8 0.08331833723624982 1108 9 0.14048511711443012 1108 10-11 0.15996189863776777 1108 12-13 0.14599258759420763 1108 14-15 0.12087775907622955 1108 16-17 0.06543678573594036 1108 18-19 0.10851504870377937 1108 20-21 0.21086399073605122 1108 22-23 0.10554157804499908 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-0.5259657809880878 2215 58-59 -0.5874700450296118 2215 60-61 -0.47050582060713 2215 62-63 -0.5565129412632288 2215 64-65 -0.6085433204362474 2215 66-67 -0.5224359518453952 2215 68-69 -0.4996218987086465 2215 70-71 -0.41823152854512813 2215 72-73 -0.36971973935403923 2215 74-75 -0.4044628827294048 2215 76-77 -0.4941295703455282 2215 78-79 -0.4821000961009325 2215 80-81 -0.5692731130851989 2215 82-83 -0.5023680687482006 2215 84-85 -0.4560568600297472 2215 86-87 -0.5136737414498924 2215 88-89 -0.44035945756847283 2215 90-91 -0.35827596660623584 2215 92-93 -0.4088312353637171 2215 94-95 -0.4832067929955173 2215 96-97 -0.49713155395490105 2215 98-99 -0.4381654444310641 2215 100 -0.4692132810037961 2216 1 -0.5365421528423262 2216 2 -0.7079042008726049 2216 3 -0.7124889077944658 2216 4 -0.785309710215941 2216 5 -0.7629422985996968 2216 6 -0.7948046217564197 2216 7 -0.7139608298696842 2216 8 -0.6825894100279868 2216 9 -0.833380784079182 2216 10-11 -0.8726617414920241 2216 12-13 -0.8494541406662819 2216 14-15 -1.0135019838414436 2216 16-17 -1.0430338987826318 2216 18-19 -1.1324882082854728 2216 20-21 -1.0349184074643034 2216 22-23 -1.0493947942013193 2216 24-25 -1.0680187543619581 2216 26-27 -1.1007335922432375 2216 28-29 -1.037842995981947 2216 30-31 -1.2050215036111922 2216 32-33 -1.2108389273758533 2216 34-35 -1.179718674274227 2216 36-37 -1.1133680820062324 2216 38-39 -1.047073953262199 2216 40-41 -1.041249668271682 2216 42-43 -1.0459257439334237 2216 44-45 -1.0681760356541048 2216 46-47 -1.0507966022864323 2216 48-49 -1.2167519542452752 2216 50-51 -1.111676001143529 2216 52-53 -1.2298585599377603 2216 54-55 -1.1336114749048392 2216 56-57 -1.1298180823513349 2216 58-59 -1.146158945882128 2216 60-61 -1.0779871220689543 2216 62-63 -1.2322713202326128 2216 64-65 -1.242626011714627 2216 66-67 -1.2168513913292287 2216 68-69 -1.1621400464568836 2216 70-71 -1.1323966959623064 2216 72-73 -1.1870568442954337 2216 74-75 -1.1821371074500284 2216 76-77 -1.15396759414174 2216 78-79 -1.1534817027415123 2216 80-81 -1.142794807179591 2216 82-83 -1.0980219142768135 2216 84-85 -1.05082134025184 2216 86-87 -1.2376133826379956 2216 88-89 -1.1584352467948662 2216 90-91 -1.266919758348358 2216 92-93 -1.225378975191827 2216 94-95 -1.1931937126653338 2216 96-97 -1.1780332020364384 2216 98-99 -1.2314857062979065 2216 100 -1.3154056358161803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119376.0 3 21387.0 4 13557.0 5 10804.0 6 9673.0 7 10415.0 8 11638.0 9 13599.0 10 14926.0 11 16603.0 12 17868.0 13 19850.0 14 21530.0 15 22384.0 16 22304.0 17 22916.0 18 21919.0 19 20860.0 20 21009.0 21 22408.0 22 24728.0 23 29808.0 24 36658.0 25 45812.0 26 54929.0 27 63846.0 28 76543.0 29 95477.0 30 113823.0 31 133824.0 32 169982.0 33 222931.0 34 305546.0 35 441020.0 36 730548.0 37 1236315.0 38 1504704.0 39 491954.0 40 824.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.34569742916936 20.79825117187569 14.901269643696175 22.95478175525877 2 15.224229170366426 20.536115200566744 40.76540492188111 23.474250707185725 3 18.14692472317856 25.734944475282596 30.57852552276016 25.539605278778684 4 12.699054120454583 17.44829569872895 39.02746520548332 30.825184975333148 5 13.099562094004932 37.85931375584359 35.54995237222876 13.491171777922723 6 29.960220384013336 38.2925725146242 18.21953894914953 13.527668152212936 7 26.382353847160882 32.82952012044157 22.94717333240923 17.84095269998832 8 24.4551856521193 36.338393921198694 20.9921541343873 18.21426629229471 9 24.645464904061406 17.427856561572234 22.1494621115105 35.777216422855865 10-11 23.373962105391495 27.38584312355593 29.302246861600818 19.937947909451754 12-13 23.900150047173497 25.10630279946347 29.11156608147788 21.881981071885157 14-15 21.475840074947826 27.380852691913432 26.337029390391216 24.80627784274753 16-17 20.977693338225166 30.46656310825394 27.09942008679207 21.456323466728826 18-19 20.92917343690671 29.43532468357466 28.96594632756353 20.6695555519551 20-21 22.10133337525732 28.67752230527164 28.4044736315241 20.81667068794694 22-23 21.812779890478247 28.55028463302513 28.412592354332624 21.224343122163997 24-25 21.604476842723418 28.64929235560848 28.352275748972676 21.39395505269542 26-27 21.32165247745374 28.706555912043726 28.42695839771856 21.544833212783978 28-29 21.304114552005146 28.6716336969318 28.640864814154266 21.383386936908792 30-31 21.612071449947344 28.99214513229447 28.169970388939834 21.225813028818347 32-33 21.310596635777294 28.774186717488554 28.542961577981036 21.372255068753123 34-35 21.575896370196094 28.5405540935805 28.441769514466092 21.44178002175731 36-37 21.71714255005478 28.714409007855725 28.11683165775538 21.451616784334107 38-39 21.513532401300537 28.821813623174545 28.313464184996455 21.35118979052846 40-41 21.50473309727003 28.522045609226605 28.421556277194306 21.551665016309062 42-43 21.42928585275911 28.619107649012644 28.35442122181982 21.597185276408425 44-45 21.53149135762182 28.725538303126946 28.222869900227586 21.52010043902365 46-47 21.566587760792473 28.770271603591436 28.156722948935254 21.50641768668084 48-49 21.48340755304373 28.604898059699035 28.207268117049356 21.704426270207875 50-51 21.43924851635689 28.605516646608837 28.173947108287578 21.781287728746694 52-53 21.4389723399924 28.656100263456906 28.18056078478492 21.724366611765774 54-55 21.556447804821627 28.826507551584292 28.067254351225813 21.549790292368268 56-57 21.451423731780256 28.628805998871982 28.08305291231556 21.83671735703221 58-59 21.415497127194104 28.37483512184261 28.294952526800373 21.91471522416291 60-61 21.51744675403799 28.687274146502972 27.999489240442465 21.79578985901657 62-63 21.363785525032473 28.65388960818477 28.124357536781886 21.857967330000875 64-65 21.450771474368512 28.43522229710417 28.17997477367018 21.934031454857138 66-67 21.314615109735747 28.476493332996444 28.067838320300925 22.141053236966886 68-69 21.553558312359712 28.67661609174602 27.964271009575715 21.80555458631855 70-71 21.526814839748884 28.363133126099488 28.073853951881627 22.036198082270005 72-73 21.356184766529424 28.433095576327105 28.243300685563856 21.96741897157962 74-75 21.357164974338325 28.520518912952447 28.0715799538908 22.050736158818424 76-77 21.42358071247213 28.44909959426297 27.955934801344462 22.171384891920432 78-79 21.454470873228438 28.426107506489238 28.193673096539207 21.925748523743113 80-81 21.354761405693225 28.37533411673937 28.149505253336716 22.120399224230685 82-83 21.260850593483948 28.37642555662682 28.365195964405398 21.997527885483827 84-85 21.246890926120408 28.199882619401713 28.221322986985705 22.331903467492175 86-87 21.44557695167656 28.29833751760112 28.121881150965887 22.134204379756433 88-89 21.32862095690407 28.19941145829072 28.182598731056203 22.289368853749007 90-91 21.25218883982465 28.14502693185419 28.196307749074528 22.406476479246628 92-93 21.348386433377144 28.189263080185935 28.125770497252155 22.336579989184767 94-95 21.214461027511287 27.998865133629153 28.29677958806308 22.489894250796482 96-97 21.256888432855156 28.007917596928962 28.249749697479267 22.485444272736622 98-99 21.228399147467957 28.011333940573653 28.193730623360313 22.566536288598073 100 21.341899468225893 27.945715729444885 28.10516775347498 22.60721704885424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2213.0 1 1730.0 2 1420.0 3 1600.5 4 1497.0 5 1334.5 6 1250.0 7 1210.0 8 1259.0 9 1336.0 10 1191.5 11 1042.5 12 1099.5 13 1192.5 14 1293.5 15 1449.0 16 1721.5 17 2179.0 18 2930.0 19 3938.0 20 5449.0 21 7715.0 22 11716.0 23 18178.0 24 25648.0 25 34576.5 26 47070.0 27 62715.5 28 82415.5 29 102924.5 30 118823.5 31 135178.5 32 152620.5 33 167663.5 34 181031.5 35 192728.0 36 202268.5 37 210528.5 38 217906.0 39 219787.5 40 222566.0 41 224130.5 42 219274.5 43 215038.5 44 210682.5 45 207117.5 46 203985.5 47 198139.5 48 193842.5 49 191404.5 50 188081.5 51 180586.5 52 167865.5 53 159370.0 54 155463.5 55 149502.0 56 137603.5 57 122412.0 58 109486.0 59 98244.0 60 86077.5 61 73159.0 62 61406.5 63 50561.0 64 41203.0 65 33454.0 66 26276.0 67 20094.0 68 15472.5 69 11785.0 70 8781.0 71 6526.5 72 4735.0 73 3360.5 74 2354.5 75 1671.0 76 1098.5 77 704.5 78 495.0 79 365.5 80 278.0 81 183.0 82 131.0 83 105.5 84 80.0 85 64.5 86 58.0 87 46.0 88 32.0 89 32.5 90 28.5 91 21.0 92 20.5 93 12.5 94 7.0 95 9.5 96 12.0 97 10.0 98 9.5 99 12.5 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07627161871312536 2 0.01262371481119446 3 0.016649829700152285 4 0.01008934766993814 5 0.00866176111568616 6 0.007250214859796564 7 0.019793728179179116 8 0.009126929768195232 9 0.009159010364919996 10-11 0.013241266298146159 12-13 0.014837275985203143 14-15 0.010073307371575758 16-17 0.012495392424295405 18-19 0.008356995446800906 20-21 0.012735996899731133 22-23 0.013361568535864022 24-25 0.015663351350865808 26-27 0.005405580548122659 28-29 0.011669317058632745 30-31 0.010386093189642202 32-33 0.012238747650497296 34-35 0.008525418579605915 36-37 0.010073307371575758 38-39 0.018991713261060028 40-41 0.012840258839086615 42-43 0.008124411120546371 44-45 0.020250876682506996 46-47 0.018173658044578555 48-49 0.017892952823236875 50-51 0.010522435725722448 52-53 0.01028183125028672 54-55 0.011845760340618945 56-57 0.02020275578741985 58-59 0.0023900044559948853 60-61 0.0071379327712598915 62-63 0.015470867770517226 64-65 0.02445343485345102 66-67 0.012840258839086615 68-69 0.013995160321178101 70-71 0.025095046787946294 72-73 0.02010651399724556 74-75 0.020339098323500095 76-77 0.009311393199362623 78-79 0.011412672284834635 80-81 0.02020275578741985 82-83 0.012318949142309205 84-85 0.011701397655357508 86-87 0.016505467014890852 88-89 0.014837275985203143 90-91 0.015607210306597471 92-93 0.008004108882828508 94-95 0.015478887919698417 96-97 0.013489890922763077 98-99 0.010137468565025284 100 0.007186053666347037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6234298.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.72595059683166 #Duplication Level Percentage of deduplicated Percentage of total 1 75.70674747633956 45.21657459618666 2 14.423393503445956 17.229017756509514 3 4.450642211198594 7.974565104906624 4 1.898001890618699 4.53439868607142 5 1.0039190787468697 2.9980010650226143 6 0.5939482297112356 2.1284473574885325 7 0.38245171280121143 1.5989604473107162 8 0.27236621799258875 1.301386502405699 9 0.18840947782799786 1.0127641648256864 >10 0.9987677063589548 10.567540656264383 >50 0.055971029365075156 2.255544009463648 >100 0.024223679839384477 2.6135880301824765 >500 0.0010500872237045715 0.4582849356297526 >1k 1.0769853012659292E-4 0.11092668773225371 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.604029836238178E-5 0.0 9 0.0 0.0 0.0 1.604029836238178E-5 0.0 10-11 0.0 0.0 0.0 4.812089508714534E-5 0.0 12-13 0.0 0.0 0.0 8.020149181190889E-5 0.0 14-15 0.0 0.0 0.0 8.020149181190889E-5 0.0 16-17 0.0 0.0 0.0 8.020149181190889E-5 0.0 18-19 0.0 1.604029836238178E-5 0.0 8.020149181190889E-5 0.0 20-21 0.0 1.604029836238178E-5 0.0 1.2030223771786335E-4 0.0 22-23 0.0 1.604029836238178E-5 0.0 2.566447737981085E-4 0.0 24-25 0.0 1.604029836238178E-5 0.0 5.373499951397896E-4 0.0 26-27 0.0 1.604029836238178E-5 0.0 0.0011468813329102972 0.0 28-29 0.0 2.406044754357267E-5 0.0 0.0034406439987308916 0.0 30-31 0.0 3.208059672476356E-5 0.0 0.01017756931093124 0.0 32-33 0.0 3.208059672476356E-5 0.0 0.0270279027406133 0.0 34-35 0.0 3.208059672476356E-5 0.0 0.054681377117359486 0.0 36-37 0.0 3.208059672476356E-5 0.0 0.09763729613181789 0.0 38-39 0.0 3.208059672476356E-5 0.0 0.15296028518367266 0.0 40-41 0.0 3.208059672476356E-5 0.0 0.2093419339274446 0.0 42-43 0.0 3.208059672476356E-5 0.0 0.2674398945959914 0.0 44-45 0.0 3.208059672476356E-5 0.0 0.34461779016659133 0.0 46-47 0.0 3.208059672476356E-5 0.0 0.4417578370491754 0.0 48-49 0.0 3.208059672476356E-5 0.0 0.5418653391288001 0.0 50-51 0.0 3.208059672476356E-5 0.0 0.6412029068870304 0.0 52-53 0.0 4.0100745905954446E-5 0.0 0.7405565149436232 0.0 54-55 0.0 6.416119344952712E-5 0.0 0.8567684765790791 0.0 56-57 0.0 1.2832238689905424E-4 0.0 0.9976824976926031 0.0 58-59 0.0 1.2832238689905424E-4 0.0 1.1540272858307383 0.0 60-61 0.0 1.2832238689905424E-4 0.0 1.3190739358304655 0.0 62-63 0.0 1.2832238689905424E-4 0.0 1.4856283738762568 0.0 64-65 0.0 1.2832238689905424E-4 0.0 1.6648065267332424 0.0 66-67 0.0 1.2832238689905424E-4 0.0 1.8744051054344852 0.0 68-69 0.0 1.2832238689905424E-4 0.0 2.109844604797525 0.0 70-71 0.0 1.2832238689905424E-4 0.0 2.3625434651984873 0.0 72-73 0.0 1.2832238689905424E-4 0.0 2.623511099405258 0.0 74-75 0.0 1.2832238689905424E-4 0.0 2.893332978308063 0.0 76-77 0.0 1.6040298362381779E-4 0.0 3.184231809259038 0.0 78-79 0.0 1.6842313280500867E-4 0.0 3.514990781640531 0.0 80-81 0.0 1.7644328198619956E-4 0.0 3.8781591768632167 0.0 82-83 0.0 1.7644328198619956E-4 0.0 4.250815408567252 0.0 84-85 0.0 1.7644328198619956E-4 0.0 4.623134794005676 0.0 86-87 0.0 1.7644328198619956E-4 0.0 5.024326716496388 0.0 88 0.0 1.7644328198619956E-4 0.0 5.343312109879894 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6625 0.0 47.773083 1 GTATCAA 11380 0.0 37.151085 1 ATCAACG 12655 0.0 32.60728 3 TCAACGC 12595 0.0 32.575516 4 CAACGCA 13860 0.0 29.772615 5 TATCAAC 14070 0.0 29.661568 2 AACGCAG 15055 0.0 27.441053 6 ACGCAGA 16790 0.0 24.157932 7 CGCAGAG 16670 0.0 24.07673 8 GTACATG 19795 0.0 22.379454 1 TACATGG 20110 0.0 21.243547 2 ACATGGG 20740 0.0 19.986744 3 GCAGAGT 19230 0.0 19.869799 9 GTGGTAT 3625 0.0 19.070557 1 CAGAGTA 17915 0.0 17.81285 10-11 CATGGGG 14135 0.0 17.588755 4 AGAGTAC 16660 0.0 17.335138 10-11 GAGTACA 15370 0.0 16.400042 1 GTCTAGG 1610 0.0 14.604886 1 AGTACAT 16330 0.0 14.505062 2 >>END_MODULE