FastQCFastQC Report
Wed 25 May 2016
SRR2049475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6234298
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179130.2873298645653448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179040.28718550188008335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95430.15307256727220933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119850.058.345721
GTATCAA217100.040.858251
ATCAACG267800.032.8573153
TCAACGC269800.032.666014
TATCAAC274450.032.1296772
GTGGTAT46550.031.9444661
CAACGCA281100.031.3528615
AACGCAG296000.029.8506746
TGGTATC53100.027.2642122
ACGCAGA334000.026.2293477
CGCAGAG333050.026.2194948
GCAGAGT367350.023.515469
GTACATG190950.022.9680671
TACATGG192350.021.7243272
GAGTACT260250.021.4091212-13
CAGAGTA360000.021.22132510-11
ACATGGG196500.020.595743
AGAGTAC342450.020.16102610-11
GTACTTT281250.019.69359814-15
AGTACTT271050.018.47532312-13
Produced by FastQC (version 0.11.5)