FastQCFastQC Report
Wed 25 May 2016
SRR2049474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647634
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69400.043.84641
GTATCAA116450.034.1680371
TCAACGC123000.031.4477584
ATCAACG123650.031.3603553
CAACGCA131850.029.4084435
AACGCAG141100.027.712666
TATCAAC141400.027.656362
ACGCAGA159200.024.0312467
CGCAGAG162350.023.2740828
GTACATG187950.020.6693531
GTGGTAT40600.020.5038971
TACATGG188000.019.801062
GCAGAGT188900.019.2074859
ACATGGG194100.018.6702423
AGAGTAC161100.017.43121110-11
CAGAGTA174300.017.0818310-11
TGGTATC43900.016.274222
CATGGGG133050.015.0837034
GAGTACA149750.014.6669291
CCGGTCG5702.5465852E-1114.0171359
Produced by FastQC (version 0.11.5)