FastQCFastQC Report
Wed 25 May 2016
SRR2049474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647634
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212040.31897062924944425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206490.3106217941601478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98840.1486844793200107No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127100.057.951371
GTATCAA230350.039.63771
GTGGTAT49400.034.3934021
ATCAACG273800.032.9450763
TCAACGC277800.032.5722274
CAACGCA287900.031.494855
TGGTATC51900.031.3370232
TATCAAC289650.031.3045652
AACGCAG303750.029.8471496
ACGCAGA339050.026.6564567
CGCAGAG342500.026.1135038
GCAGAGT375300.023.4180099
GAGTACT265300.022.0821812-13
CAGAGTA367600.021.49850710-11
GTACATG181950.020.8227831
GTACTTT291600.020.0502514-15
AGAGTAC349050.019.94807210-11
TACATGG183150.019.7620872
AGTACTT278900.018.62932612-13
ACATGGG185950.018.5544833
Produced by FastQC (version 0.11.5)