##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049474_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6647634 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82233393114001 31.0 31.0 34.0 30.0 34.0 2 32.089900105812085 33.0 31.0 34.0 30.0 34.0 3 32.07462128631029 34.0 31.0 34.0 30.0 34.0 4 35.78245839045892 37.0 35.0 37.0 33.0 37.0 5 35.857361280720326 37.0 35.0 37.0 35.0 37.0 6 35.93832166452004 37.0 35.0 37.0 35.0 37.0 7 35.90983679306051 37.0 35.0 37.0 35.0 37.0 8 35.91520667353227 37.0 35.0 37.0 35.0 37.0 9 37.63099066525022 39.0 37.0 39.0 35.0 39.0 10-11 37.60614220036783 39.0 37.0 39.0 35.0 39.0 12-13 37.59538160494395 39.0 37.0 39.0 35.0 39.0 14-15 38.88330773023905 40.0 38.0 41.0 35.0 41.0 16-17 38.88194446324813 40.0 38.0 41.0 35.0 41.0 18-19 38.96506478846459 40.0 38.5 41.0 35.0 41.0 20-21 38.993618932690936 40.0 39.0 41.0 35.0 41.0 22-23 38.96667739228724 40.0 38.5 41.0 35.0 41.0 24-25 38.94879997906022 40.0 38.0 41.0 35.0 41.0 26-27 38.85064678651081 40.0 38.0 41.0 35.0 41.0 28-29 38.772136973846635 40.0 38.0 41.0 35.0 41.0 30-31 38.66530182016639 40.0 38.0 41.0 35.0 41.0 32-33 38.53213752441846 40.0 38.0 41.0 34.5 41.0 34-35 38.419475635993194 40.0 38.0 41.0 34.0 41.0 36-37 38.28658655094429 40.0 38.0 41.0 34.0 41.0 38-39 38.18245280952591 40.0 38.0 41.0 34.0 41.0 40-41 38.09441344093252 40.0 38.0 41.0 34.0 41.0 42-43 37.9566942915329 40.0 38.0 41.0 33.0 41.0 44-45 37.783988483722176 40.0 37.5 41.0 33.0 41.0 46-47 37.53687274299398 40.0 37.0 41.0 33.0 41.0 48-49 37.2904637950886 40.0 37.0 41.0 32.5 41.0 50-51 37.130293800771824 40.0 37.0 41.0 32.0 41.0 52-53 37.01710540923282 40.0 37.0 41.0 31.5 41.0 54-55 36.87857108258367 40.0 36.0 41.0 31.0 41.0 56-57 36.733061642683694 40.0 36.0 41.0 31.0 41.0 58-59 36.56268395642721 39.0 35.5 41.0 31.0 41.0 60-61 36.378763557079104 39.0 35.0 41.0 31.0 41.0 62-63 36.165759877875345 39.0 35.0 41.0 31.0 41.0 64-65 35.94797976242374 38.5 35.0 40.5 30.0 41.0 66-67 35.68659473430697 38.0 35.0 40.0 30.0 41.0 68-69 35.39517082318311 37.0 35.0 40.0 30.0 41.0 70-71 35.109361992552536 37.0 35.0 40.0 29.0 41.0 72-73 34.777665030896706 36.5 34.5 39.0 29.0 41.0 74-75 34.451264840994554 36.0 34.0 39.0 29.0 41.0 76-77 34.12539897653812 35.5 34.0 38.5 29.0 40.0 78-79 33.81284423901798 35.0 34.0 37.0 29.0 39.5 80-81 33.498316468686454 35.0 34.0 37.0 29.0 39.0 82-83 33.21927899460169 35.0 34.0 36.5 29.0 39.0 84-85 32.930693461763994 35.0 34.0 36.0 28.5 37.5 86-87 32.68086660607368 35.0 34.0 36.0 28.0 37.0 88-89 32.460392073330155 35.0 34.0 35.5 27.0 37.0 90-91 32.26470011736507 35.0 33.0 35.0 27.0 36.0 92-93 32.09993232178546 35.0 33.0 35.0 27.0 36.0 94-95 31.939469065234338 35.0 33.0 35.0 27.0 36.0 96-97 31.7931337675931 35.0 33.0 35.0 26.0 36.0 98-99 31.653525299377193 35.0 33.0 35.0 26.0 35.0 100 31.542641637611215 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3961457080253723 1101 2 0.4387117215080636 1101 3 0.5605644982250197 1101 4 0.3573324787740546 1101 5 0.3007843923004003 1101 6 0.28944088924726685 1101 7 0.30003355431149714 1101 8 0.3016767983201163 1101 9 0.3808398220305165 1101 10-11 0.3395305201463543 1101 12-13 0.3196749399423737 1101 14-15 0.5009571885233584 1101 16-17 0.4840719150850106 1101 18-19 0.4903302646383736 1101 20-21 0.5110444150681346 1101 22-23 0.48712749662691834 1101 24-25 0.47777418049278 1101 26-27 0.47035624757505445 1101 28-29 0.4734887311403355 1101 30-31 0.4752679875606418 1101 32-33 0.47680679982745744 1101 34-35 0.5021036955713285 1101 36-37 0.5529357917918176 1101 38-39 0.4776500975512832 1101 40-41 0.5210880986324895 1101 42-43 0.5204037668360542 1101 44-45 0.509825612597254 1101 46-47 0.4984740098933287 1101 48-49 0.4853237245498221 1101 50-51 0.4245959250592932 1101 52-53 0.46156213552764314 1101 54-55 0.4857072660436046 1101 56-57 0.4900926148527702 1101 58-59 0.44098726881367156 1101 60-61 0.3913361158378734 1101 62-63 0.4467166504421982 1101 64-65 0.4554417385615963 1101 66-67 0.4556799507388192 1101 68-69 0.41964031394442713 1101 70-71 0.43608046659016964 1101 72-73 0.4202160559367769 1101 74-75 0.3743240817712916 1101 76-77 0.4080634477275993 1101 78-79 0.355954624304502 1101 80-81 0.290261501861238 1101 82-83 0.27013797537247797 1101 84-85 0.2646875777944899 1101 86-87 0.28344838383276993 1101 88-89 0.3653187220007652 1101 90-91 0.3447531419215153 1101 92-93 0.34119294542308864 1101 94-95 0.34694525603243065 1101 96-97 0.3132200978157407 1101 98-99 0.36133327323080167 1101 100 0.3944893369702811 1102 1 0.3385663435720687 1102 2 0.3780380164545747 1102 3 0.4280374941419538 1102 4 0.30631766244000147 1102 5 0.27824196983397087 1102 6 0.25734996675230093 1102 7 0.2654029128261115 1102 8 0.2569163510311796 1102 9 0.32576190329412213 1102 10-11 0.315494173975992 1102 12-13 0.3617668976660866 1102 14-15 0.4672439612995092 1102 16-17 0.4722760532341965 1102 18-19 0.4835185958282011 1102 20-21 0.46207780319165437 1102 22-23 0.400584686363608 1102 24-25 0.429923628958349 1102 26-27 0.3722283949744849 1102 28-29 0.43929185342140187 1102 30-31 0.42298355520002673 1102 32-33 0.4283751859866669 1102 34-35 0.4503202973682079 1102 36-37 0.46910921529815397 1102 38-39 0.4379475412666807 1102 40-41 0.4517018188511628 1102 42-43 0.412689311361504 1102 44-45 0.49175658592069027 1102 46-47 0.4504458236238307 1102 48-49 0.43729376095910055 1102 50-51 0.45130183205699836 1102 52-53 0.48433446545066516 1102 54-55 0.5024897069279533 1102 56-57 0.46036720952127297 1102 58-59 0.44772954905543827 1102 60-61 0.42305986324517875 1102 62-63 0.4296167264239017 1102 64-65 0.3968522226337754 1102 66-67 0.4380944284761412 1102 68-69 0.46100895062315317 1102 70-71 0.4215910135551795 1102 72-73 0.4258924060338032 1102 74-75 0.39884943474728374 1102 76-77 0.4221216203764868 1102 78-79 0.3842540823822205 1102 80-81 0.3961037807201109 1102 82-83 0.3612141234099937 1102 84-85 0.25971782244542396 1102 86-87 0.30495837744220466 1102 88-89 0.3201027448470839 1102 90-91 0.3094679105941509 1102 92-93 0.2837721521086962 1102 94-95 0.283450090054 1102 96-97 0.33076627554279625 1102 98-99 0.39630557428817426 1102 100 0.41564028734532954 1103 1 0.3304298114122375 1103 2 0.3346915144004541 1103 3 0.4130941638427146 1103 4 0.2268278842914171 1103 5 0.22478020047169167 1103 6 0.25650019009545844 1103 7 0.27821526473973535 1103 8 0.26817300414372625 1103 9 0.2328928592944166 1103 10-11 0.2826474416761897 1103 12-13 0.2670275546119285 1103 14-15 0.358388788904179 1103 16-17 0.34956363653238753 1103 18-19 0.3616688965737893 1103 20-21 0.35875938524927875 1103 22-23 0.3575266145111655 1103 24-25 0.34817456647621725 1103 26-27 0.3229854560305938 1103 28-29 0.4151105814119589 1103 30-31 0.3820662178084717 1103 32-33 0.36013879546359107 1103 34-35 0.378781432095181 1103 36-37 0.36844728261075943 1103 38-39 0.3119988480453699 1103 40-41 0.31973833502741655 1103 42-43 0.34821043486260095 1103 44-45 0.3551078435855217 1103 46-47 0.36070662203565007 1103 48-49 0.4019506849663017 1103 50-51 0.3830044242352315 1103 52-53 0.3406984981623964 1103 54-55 0.3540331538062276 1103 56-57 0.34142206291890886 1103 58-59 0.32072535926247525 1103 60-61 0.28328213237143984 1103 62-63 0.3879501674529138 1103 64-65 0.3430124471829785 1103 66-67 0.29105201009935655 1103 68-69 0.3189389170724084 1103 70-71 0.2769458549939756 1103 72-73 0.33376020822531416 1103 74-75 0.3297647156897128 1103 76-77 0.3070270426404278 1103 78-79 0.2829850056491736 1103 80-81 0.22557780260519422 1103 82-83 0.2264566057857138 1103 84-85 0.23264088267804084 1103 86-87 0.23914123973229806 1103 88-89 0.2596366384564561 1103 90-91 0.24234992053904847 1103 92-93 0.2962510262536924 1103 94-95 0.30676244826782195 1103 96-97 0.33031512485208125 1103 98-99 0.37146131609675237 1103 100 0.32669080860706856 1104 1 0.27592421675957723 1104 2 0.23268758782407417 1104 3 0.3979901478393444 1104 4 0.21141597863332606 1104 5 0.21731228541198533 1104 6 0.22331128066795003 1104 7 0.19893460669199925 1104 8 0.19905735597021135 1104 9 0.2588669003340982 1104 10-11 0.24862709870802036 1104 12-13 0.22247038203417446 1104 14-15 0.33036950454699365 1104 16-17 0.3204536684302539 1104 18-19 0.2919109590712168 1104 20-21 0.2932139224548749 1104 22-23 0.2913229641096322 1104 24-25 0.2719294465377402 1104 26-27 0.26691309104617034 1104 28-29 0.2820240076204783 1104 30-31 0.33368603966666655 1104 32-33 0.2968129764512071 1104 34-35 0.2940033726646334 1104 36-37 0.294230402897405 1104 38-39 0.3172411146572287 1104 40-41 0.31494009329080086 1104 42-43 0.3619729190296397 1104 44-45 0.3706500676167437 1104 46-47 0.3245788623241026 1104 48-49 0.33423215736162604 1104 50-51 0.3402381722425787 1104 52-53 0.38889766905007406 1104 54-55 0.3296222869277088 1104 56-57 0.3339968599808145 1104 58-59 0.3719914781305249 1104 60-61 0.3656394110489316 1104 62-63 0.33898331633491807 1104 64-65 0.26326903213318786 1104 66-67 0.30990531293127077 1104 68-69 0.2890787358543605 1104 70-71 0.3450386329255153 1104 72-73 0.33652180762391026 1104 74-75 0.309782734548385 1104 76-77 0.2851464380900097 1104 78-79 0.26805084932345125 1104 80-81 0.26608704599554045 1104 82-83 0.2658296194607601 1104 84-85 0.23567597886157898 1104 86-87 0.22471986640103836 1104 88-89 0.26553393290649296 1104 90-91 0.28436039667568025 1104 92-93 0.266555280074968 1104 94-95 0.24562099079212985 1104 96-97 0.28113826510405104 1104 98-99 0.32389131486808864 1104 100 0.2927989058276772 1105 1 0.2834719738730769 1105 2 0.255672784715145 1105 3 0.3522131188513171 1105 4 0.17920042441281936 1105 5 0.1789486014244801 1105 6 0.13236815386127887 1105 7 0.13769383596097384 1105 8 0.12681928001158838 1105 9 0.1608909907577143 1105 10-11 0.1473844850254835 1105 12-13 0.15441504415005625 1105 14-15 0.22765298313973403 1105 16-17 0.24324443030931064 1105 18-19 0.24493528603112935 1105 20-21 0.2677251412407955 1105 22-23 0.19525104474093524 1105 24-25 0.26259442552519374 1105 26-27 0.2178880414170834 1105 28-29 0.2446801143201185 1105 30-31 0.2527141693466035 1105 32-33 0.25711061311984196 1105 34-35 0.27548316114604177 1105 36-37 0.26746978561023127 1105 38-39 0.3030255372184385 1105 40-41 0.3155301642807089 1105 42-43 0.30608550522752864 1105 44-45 0.3443313233487615 1105 46-47 0.32247733528640765 1105 48-49 0.34166391373794625 1105 50-51 0.2771882797722611 1105 52-53 0.26835630567228463 1105 54-55 0.33115133808339436 1105 56-57 0.2650373693609964 1105 58-59 0.28593979132199365 1105 60-61 0.25694028086400067 1105 62-63 0.25545507636812204 1105 64-65 0.25350900763789497 1105 66-67 0.2307405690289741 1105 68-69 0.2538175047337319 1105 70-71 0.2858869638209214 1105 72-73 0.2354943258792872 1105 74-75 0.19965051003335788 1105 76-77 0.21255818320545927 1105 78-79 0.21073424113784966 1105 80-81 0.20115774205075354 1105 82-83 0.1579121111312034 1105 84-85 0.17631570796221752 1105 86-87 0.14891986944842728 1105 88-89 0.15149242006097552 1105 90-91 0.17494666929899694 1105 92-93 0.1591264441237783 1105 94-95 0.16029895678314077 1105 96-97 0.18101357564863818 1105 98-99 0.23308396062088477 1105 100 0.20466754982168922 1106 1 0.26179795962843144 1106 2 0.25270362617778375 1106 3 0.31398580451864433 1106 4 0.18195712726968338 1106 5 0.20907917654756858 1106 6 0.2070813691653015 1106 7 0.15554023501393033 1106 8 0.14594705619666115 1106 9 0.1543378767563297 1106 10-11 0.205119692031829 1106 12-13 0.18924002032446197 1106 14-15 0.3090542963588092 1106 16-17 0.31476813896557587 1106 18-19 0.27324247622370024 1106 20-21 0.2852359139505154 1106 22-23 0.2684133721760631 1106 24-25 0.26220939277395416 1106 26-27 0.24105668076460773 1106 28-29 0.2666633858629339 1106 30-31 0.24219575432692864 1106 32-33 0.27661694152813254 1106 34-35 0.25562869227142926 1106 36-37 0.30495560767759855 1106 38-39 0.24246792820905938 1106 40-41 0.23654319310804794 1106 42-43 0.19469318186193618 1106 44-45 0.24028753277536197 1106 46-47 0.2686589980168108 1106 48-49 0.3084315527194761 1106 50-51 0.28461377645293595 1106 52-53 0.24981175669427103 1106 54-55 0.33768944935768275 1106 56-57 0.37571776914009547 1106 58-59 0.31741805217743035 1106 60-61 0.298952934592684 1106 62-63 0.28887547427498816 1106 64-65 0.2625152351206026 1106 66-67 0.2898939146554014 1106 68-69 0.3101820800629014 1106 70-71 0.3031922286262656 1106 72-73 0.3480680048104432 1106 74-75 0.33075080983599037 1106 76-77 0.28996801935495853 1106 78-79 0.2439104104932639 1106 80-81 0.19736719182264295 1106 82-83 0.2367496644868723 1106 84-85 0.2563790968712567 1106 86-87 0.23265425401685746 1106 88-89 0.21394039300056988 1106 90-91 0.2167863091815363 1106 92-93 0.2358878313256909 1106 94-95 0.22304532858131765 1106 96-97 0.24761912220052906 1106 98-99 0.2678256752150219 1106 100 0.23059524858171798 1107 1 0.1354337345289487 1107 2 0.1262578800447045 1107 3 0.2277404641456826 1107 4 0.11798398285931455 1107 5 0.13682489212468596 1107 6 0.14827746830998478 1107 7 0.10314199146985459 1107 8 0.1321281404250385 1107 9 0.13479966323132686 1107 10-11 0.11414461535872533 1107 12-13 0.10531304990492885 1107 14-15 0.16768190516639692 1107 16-17 0.149239601996058 1107 18-19 0.17126376224310036 1107 20-21 0.17363405430439371 1107 22-23 0.20027901091659572 1107 24-25 0.19697775456744182 1107 26-27 0.1932833153425264 1107 28-29 0.20870247882311332 1107 30-31 0.22511437813918178 1107 32-33 0.1590566016313062 1107 34-35 0.16266369235331268 1107 36-37 0.1957315247383633 1107 38-39 0.1878349733121567 1107 40-41 0.20255234122957688 1107 42-43 0.17156974019475513 1107 44-45 0.283899466124204 1107 46-47 0.20935443201129544 1107 48-49 0.1529440435360101 1107 50-51 0.12280051681078419 1107 52-53 0.1086164851842284 1107 54-55 0.1276446756933467 1107 56-57 0.10623452092497843 1107 58-59 0.15005821704382072 1107 60-61 0.13918718043527178 1107 62-63 0.11292568184008189 1107 64-65 0.08017964958406054 1107 66-67 0.16685756832696796 1107 68-69 0.08026583297079526 1107 70-71 0.10413300855041285 1107 72-73 0.06882687572818469 1107 74-75 0.07713127790113816 1107 76-77 0.0679721721633939 1107 78-79 0.026992781720899472 1107 80-81 0.03676425000998762 1107 82-83 0.07393336742559597 1107 84-85 0.049194544384164374 1107 86-87 0.0981164031445303 1107 88-89 0.09678221407929755 1107 90-91 0.07298826858600194 1107 92-93 0.029437711642486875 1107 94-95 0.029383905420317546 1107 96-97 0.05201801595628197 1107 98-99 0.08935351458440266 1107 100 0.10375128887942253 1108 1 0.08992444616634288 1108 2 0.1495625870128734 1108 3 0.22630605312647134 1108 4 0.13437551981330387 1108 5 0.1380342284289 1108 6 0.1570511248757711 1108 7 0.09378551176172323 1108 8 0.1110287246590147 1108 9 0.1143978463985178 1108 10-11 0.14934197091839252 1108 12-13 0.1264631478118048 1108 14-15 0.18198380967324113 1108 16-17 0.21844189154018068 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9 -0.45463489336854934 2215 10-11 -0.4704367390713884 2215 12-13 -0.4753688009125199 2215 14-15 -0.6497368019086593 2215 16-17 -0.699433424101187 2215 18-19 -0.5854410376249461 2215 20-21 -0.6010639667796696 2215 22-23 -0.5683222091730258 2215 24-25 -0.5650007953797029 2215 26-27 -0.49420821181288943 2215 28-29 -0.5239462283358094 2215 30-31 -0.5628013440292889 2215 32-33 -0.5771833208620194 2215 34-35 -0.5684324541957011 2215 36-37 -0.5893480723469082 2215 38-39 -0.576200187309091 2215 40-41 -0.6685788378472779 2215 42-43 -0.6000618164272282 2215 44-45 -0.6355821165103919 2215 46-47 -0.6529225323479864 2215 48-49 -0.607965193395863 2215 50-51 -0.6009706824560368 2215 52-53 -0.6942323602850564 2215 54-55 -0.615058588070525 2215 56-57 -0.5590421671693804 2215 58-59 -0.5860762825494064 2215 60-61 -0.5403121827829622 2215 62-63 -0.5669569068360119 2215 64-65 -0.6109128269874233 2215 66-67 -0.5454138300691724 2215 68-69 -0.544616195296669 2215 70-71 -0.5075937133336552 2215 72-73 -0.48695241121347976 2215 74-75 -0.5034983189426754 2215 76-77 -0.5429288937312577 2215 78-79 -0.5473103736718983 2215 80-81 -0.5598407157949197 2215 82-83 -0.5599290628060771 2215 84-85 -0.5504447862871658 2215 86-87 -0.5100571629019797 2215 88-89 -0.5299312702161671 2215 90-91 -0.5036009981529972 2215 92-93 -0.5136714734928844 2215 94-95 -0.5256246099949884 2215 96-97 -0.48396606284758903 2215 98-99 -0.5364921369369959 2215 100 -0.528738457930249 2216 1 -0.7202056524018765 2216 2 -0.6879304353670577 2216 3 -0.6042709616411877 2216 4 -0.5009378126687594 2216 5 -0.4889502198983422 2216 6 -0.4569063337534587 2216 7 -0.4997318764967744 2216 8 -0.48875274330924157 2216 9 -0.6506595355574305 2216 10-11 -0.7038122208667374 2216 12-13 -0.7096072418044344 2216 14-15 -0.9635254328544818 2216 16-17 -0.9484721889896122 2216 18-19 -0.9323650852679037 2216 20-21 -0.8978377290412354 2216 22-23 -0.871137513621548 2216 24-25 -0.9635140244246259 2216 26-27 -0.9739853082622858 2216 28-29 -1.006963695945096 2216 30-31 -1.000807381437042 2216 32-33 -0.952652266161131 2216 34-35 -0.9666866761086084 2216 36-37 -1.0765277432233802 2216 38-39 -1.0133382701868285 2216 40-41 -1.1006506902124755 2216 42-43 -1.1462862759538197 2216 44-45 -1.189089268184098 2216 46-47 -1.1034845389169448 2216 48-49 -1.0901668458040135 2216 50-51 -1.1154351914853393 2216 52-53 -1.0537318877089348 2216 54-55 -1.0610662945216376 2216 56-57 -1.02692074223458 2216 58-59 -0.9851210582032763 2216 60-61 -1.0228684622296242 2216 62-63 -1.0721319322030638 2216 64-65 -1.1360517975090048 2216 66-67 -1.0634034662099268 2216 68-69 -1.0678608243528416 2216 70-71 -1.0198047701529376 2216 72-73 -1.0325330771478107 2216 74-75 -1.041759212805232 2216 76-77 -0.8655881392144948 2216 78-79 -0.8723500609691399 2216 80-81 -0.8898595018737225 2216 82-83 -0.8795792135138356 2216 84-85 -0.9616563746530531 2216 86-87 -0.9000895105672484 2216 88-89 -0.9771412515957891 2216 90-91 -0.9087645543297711 2216 92-93 -0.9641799355134566 2216 94-95 -0.9545288620754455 2216 96-97 -0.9831299572517622 2216 98-99 -1.0847638063150065 2216 100 -1.1624718158264962 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 449.0 3 36.0 4 60.0 5 161.0 6 463.0 7 1111.0 8 2226.0 9 3594.0 10 5185.0 11 7149.0 12 10314.0 13 14529.0 14 17855.0 15 23054.0 16 29154.0 17 41764.0 18 42526.0 19 22590.0 20 15706.0 21 14951.0 22 15444.0 23 17110.0 24 20245.0 25 24934.0 26 31860.0 27 41450.0 28 54108.0 29 71588.0 30 93492.0 31 120722.0 32 157355.0 33 207817.0 34 285020.0 35 420412.0 36 698816.0 37 1342049.0 38 1981490.0 39 808652.0 40 2193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.73136535177883 21.275898117441198 15.50261035161089 22.49012617916909 2 15.452941602982353 21.422358691829306 39.4765114926604 23.648188212527945 3 17.531124607642358 26.22657926113261 31.636458926589523 24.605837204635513 4 12.763668998624173 18.651478104841512 38.75387844758 29.83097444895432 5 12.515434227810179 37.66389393437668 35.79552301702801 14.025148820785127 6 29.071301823748257 38.978414556289856 17.97957105903038 13.970712560931508 7 25.286229657047905 33.66821939956382 23.233363930685716 17.812187012702566 8 24.385683688361905 35.67156374734229 21.053716254535072 18.889036309760737 9 24.471954984284633 16.963148091486385 22.433951688676 36.13094523555298 10-11 23.100015734921627 27.422711599344968 29.259605748451257 20.21766691728215 12-13 24.180257216326893 25.117207114591444 29.375639513246366 21.326896155835296 14-15 21.612674524499994 27.065208463642854 27.298313956514452 24.0238030553427 16-17 20.283630235960644 30.25259814243684 27.9949903980875 21.468781223515013 18-19 20.134975842532846 28.415952201941323 31.07774886523536 20.37132309029047 20-21 21.228755975434268 27.665768001066244 30.89408201474389 20.211394008755594 22-23 20.97271751122279 27.57005725646147 30.970063935529545 20.487161296786194 24-25 20.805236870742284 27.60786018002796 30.94863525880035 20.63826769042941 26-27 20.619915296179062 27.595419663597603 30.990778072318665 20.79388696790467 28-29 20.661005103469897 27.525575264823544 31.142508748225307 20.67091088348125 30-31 20.99501867882618 27.861867846515015 30.690942070517117 20.452171404141684 32-33 20.60886625226359 27.71081410318318 30.98340702872631 20.696912615826925 34-35 20.829298063040174 27.425517108793894 30.94604035059692 20.799144477569012 36-37 21.159588509235014 27.715372115853548 30.39719094041579 20.72784843449564 38-39 20.965707498337004 27.808518038147106 30.529959982754768 20.695814480761126 40-41 20.871215232366886 27.610334744662534 30.626039279539157 20.892410743431423 42-43 20.869342385576584 27.632297445978526 30.410458818882024 21.08790134956287 44-45 21.073587986342208 27.771286746532674 30.17739093337569 20.97773433374942 46-47 21.175864976922618 27.952727241000332 29.733646587643065 21.137761194433992 48-49 21.173533320276057 27.89797843864449 29.568956413665376 21.359531827414084 50-51 21.197767506454177 27.951614062988426 29.367945046312716 21.48267338424468 52-53 21.214322268644754 28.023564474217444 29.284689861084413 21.477423396053393 54-55 21.302406239573358 28.326807703312184 29.10911611559842 21.261669941516033 56-57 21.157505061199217 28.052755311137766 29.14102220429103 21.648717423371984 58-59 21.02573637477635 27.765277089562996 29.4341039232906 21.774882612370057 60-61 21.21105794933957 27.99104914620751 29.196274945341454 21.601617959111465 62-63 21.043735259793184 27.979338212663336 29.28887180010211 21.688054727441372 64-65 21.03552181121885 27.78220040393319 29.415458191591178 21.766819593256788 66-67 20.988911242706802 27.815798824062814 29.29475359202989 21.900536341200493 68-69 21.297810619537717 27.946003044090574 29.10317415188622 21.653012184485487 70-71 21.277811022688674 27.712732078811804 29.164436549906327 21.8450203485932 72-73 21.044765701601502 27.867381086263173 29.252979330691193 21.834873881444135 74-75 21.122065468861965 27.84861710562112 29.088404166685784 21.94091325883113 76-77 21.17272852265934 27.89359341985434 28.911797791515 22.021880265971323 78-79 21.211877789902392 27.8884336893397 29.03038885714827 21.869299663609638 80-81 21.058763569890104 27.770501993043528 29.07735277366854 22.093381663397828 82-83 20.925753585823937 27.797639163183767 29.173293431216273 22.103313819776023 84-85 20.968996813439784 27.688399750466598 29.054402584268296 22.288200851825327 86-87 21.03357722077826 27.779242800636982 28.947152672497538 22.240027306087217 88-89 20.89550397718875 27.695028166190866 28.96677333427603 22.44269452234435 90-91 20.765361064066106 27.580277346941063 29.001663684510238 22.65269790448259 92-93 20.841460299637976 27.706373798028828 28.83016663703201 22.621999265301184 94-95 20.675826786441856 27.481690629000255 29.03907495922789 22.803407625329996 96-97 20.709173235453395 27.55666706781395 28.96516035862567 22.768999338106983 98-99 20.62993635033483 27.55869615628169 28.89214772847874 22.919219764904735 100 20.702746693182384 27.445916815466564 28.870349282375823 22.98098720897523 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 782.0 1 656.5 2 1132.5 3 1693.5 4 1078.5 5 652.5 6 1607.5 7 3791.5 8 6896.5 9 8694.0 10 6751.5 11 4165.5 12 3722.5 13 4114.0 14 4686.0 15 5292.5 16 5959.5 17 6662.0 18 7536.5 19 8675.5 20 10106.5 21 12371.5 22 15743.0 23 20843.0 24 27749.5 25 36502.0 26 49038.5 27 64794.5 28 83808.5 29 105880.0 30 127032.0 31 146982.0 32 165435.0 33 181623.5 34 196636.0 35 209038.0 36 218788.0 37 228880.0 38 239036.5 39 243681.0 40 247021.5 41 247821.5 42 243310.5 43 238717.5 44 233974.5 45 228873.0 46 222015.5 47 213816.5 48 207442.0 49 202970.5 50 196836.0 51 186750.5 52 172778.0 53 161026.5 54 153497.0 55 146555.5 56 135843.5 57 121640.5 58 108913.0 59 97312.0 60 84011.5 61 69740.5 62 57523.5 63 46867.5 64 37211.0 65 29288.0 66 23023.5 67 17980.0 68 13470.5 69 9816.5 70 7150.5 71 5145.5 72 3655.5 73 2601.5 74 1857.0 75 1214.0 76 733.5 77 437.5 78 273.5 79 164.0 80 86.0 81 53.5 82 34.0 83 21.5 84 13.0 85 4.0 86 2.0 87 2.0 88 1.0 89 1.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12195316408815528 2 0.0 3 0.0 4 0.0 5 0.01325283552012641 6 9.025767664104251E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.52147305342021E-6 76-77 0.0 78-79 0.0 80-81 1.8051535328208503E-4 82-83 5.039386945791541E-4 84-85 4.061595448846913E-4 86-87 4.8137427541889344E-4 88-89 5.490675328996753E-4 90-91 5.941963712201966E-4 92-93 1.8051535328208503E-4 94-95 2.0307977244234565E-4 96-97 5.114601676325742E-4 98-99 0.0011056565388527708 100 6.017178442736168E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6647634.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.136392385114945 #Duplication Level Percentage of deduplicated Percentage of total 1 74.50810095367886 44.061402938665104 2 15.492646412809172 18.323584347034533 3 4.638067445678576 8.228357291288281 4 1.9078973913706114 4.513046750665187 5 0.9656030476127706 2.855114035594582 6 0.5963450620982306 2.1159417353500016 7 0.3858232006417837 1.5971334529103418 8 0.2653821704310051 1.2554995330097094 9 0.19772914045977993 1.0523689232580946 >10 0.966203079275662 10.000628353093543 >50 0.05190898147199413 2.092013353198947 >100 0.02299643266520593 2.3800461566287376 >500 8.13633923211703E-4 0.2998753179492791 >1k 3.813535924361824E-4 0.35459065787389904 >5k 5.0847145326736454E-5 0.241154722363929 >10k+ 5.0847145326736454E-5 0.6292424311158056 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21204 0.31897062924944425 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20649 0.3106217941601478 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9884 0.1486844793200107 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.0085892213680836E-5 0.0 4 0.0 0.0 0.0 6.017178442736167E-5 0.0 5 0.0 0.0 0.0 7.521473053420209E-5 0.0 6 0.0 0.0 0.0 7.521473053420209E-5 0.0 7 0.0 0.0 0.0 7.521473053420209E-5 0.0 8 0.0 0.0 0.0 7.521473053420209E-5 0.0 9 0.0 0.0 0.0 9.025767664104251E-5 0.0 10-11 0.0 2.2564419160260628E-5 0.0 1.0530062274788294E-4 0.0 12-13 0.0 3.0085892213680836E-5 0.0 1.2034356885472336E-4 0.0 14-15 0.0 3.7607365267101045E-5 0.0 1.4290798801498396E-4 0.0 16-17 0.0 4.5128838320521256E-5 0.0 1.6547240717524462E-4 0.0 18-19 0.0 4.5128838320521256E-5 0.0 1.8051535328208503E-4 0.0 20-21 0.0 4.5128838320521256E-5 0.0 2.1060124549576584E-4 0.0 22-23 0.0 4.5128838320521256E-5 0.0 3.911165987778509E-4 0.0 24-25 0.0 5.265031137394146E-5 0.0 7.822331975557018E-4 0.0 26-27 0.0 7.521473053420209E-5 0.0 0.0016848099639661268 0.0 28-29 0.0 7.521473053420209E-5 0.0 0.004392540263197402 0.0 30-31 0.0 7.521473053420209E-5 0.0 0.011395031675931616 0.0 32-33 0.0 7.521473053420209E-5 0.0 0.029424002584979857 0.0 34-35 0.0 7.521473053420209E-5 0.0 0.061518128103923896 0.0 36-37 0.0 7.521473053420209E-5 0.0 0.11242345772947186 0.0 38-39 0.0 9.025767664104251E-5 0.0 0.1788380647911723 0.0 40-41 0.0 9.025767664104251E-5 0.0 0.24959256180469624 0.0 42-43 0.0 9.025767664104251E-5 0.0 0.3248674641233257 0.0 44-45 0.0 9.025767664104251E-5 0.0 0.42306781630877993 0.0 46-47 0.0 9.025767664104251E-5 0.0 0.5458182565405978 0.0 48-49 0.0 9.025767664104251E-5 0.0 0.6761954704485836 0.0 50-51 0.0 9.025767664104251E-5 0.0 0.8065425984643559 0.0 52-53 0.0 9.025767664104251E-5 0.0 0.9368972479531815 0.0 54-55 0.0 9.025767664104251E-5 0.0 1.0878456906622715 0.0 56-57 0.0 9.025767664104251E-5 0.0 1.274934811393046 0.0 58-59 0.0 1.0530062274788292E-4 0.0 1.4821664369608796 0.0 60-61 3.0085892213680836E-5 1.2034356885472335E-4 0.0 1.692797768348859 0.0 62-63 3.0085892213680836E-5 1.2034356885472335E-4 0.0 1.9071898362635489 0.0 64-65 3.0085892213680836E-5 1.3538651496156377E-4 0.0 2.1456581394222365 0.0 66-67 3.0085892213680836E-5 1.504294610684042E-4 0.0 2.426382078195039 0.0 68-69 3.0085892213680836E-5 1.8051535328208503E-4 0.0 2.7353491482834347 0.0 70-71 3.0085892213680836E-5 1.8051535328208503E-4 0.0 3.0684375824541483 0.0 72-73 3.0085892213680836E-5 1.9555829938892544E-4 0.0 3.410055367067441 0.0 74-75 3.0085892213680836E-5 2.1060124549576587E-4 0.0 3.7651892387577295 0.0 76-77 3.0085892213680836E-5 2.1060124549576587E-4 0.0 4.148919750997122 0.0 78-79 3.0085892213680836E-5 2.1060124549576587E-4 0.0 4.580389052706572 0.0 80-81 3.0085892213680836E-5 2.1060124549576587E-4 0.0 5.041793817168635 0.0 82-83 3.0085892213680836E-5 2.1060124549576587E-4 0.0 5.518083576803416 0.0 84-85 3.0085892213680836E-5 2.1060124549576587E-4 0.0 5.994711802725601 0.0 86-87 3.0085892213680836E-5 2.181227185491861E-4 0.0 6.503953135807416 0.0 88 3.0085892213680836E-5 2.2564419160260628E-4 0.0 6.915242325314541 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12710 0.0 57.95137 1 GTATCAA 23035 0.0 39.6377 1 GTGGTAT 4940 0.0 34.393402 1 ATCAACG 27380 0.0 32.945076 3 TCAACGC 27780 0.0 32.572227 4 CAACGCA 28790 0.0 31.49485 5 TGGTATC 5190 0.0 31.337023 2 TATCAAC 28965 0.0 31.304565 2 AACGCAG 30375 0.0 29.847149 6 ACGCAGA 33905 0.0 26.656456 7 CGCAGAG 34250 0.0 26.113503 8 GCAGAGT 37530 0.0 23.418009 9 GAGTACT 26530 0.0 22.08218 12-13 CAGAGTA 36760 0.0 21.498507 10-11 GTACATG 18195 0.0 20.822783 1 GTACTTT 29160 0.0 20.05025 14-15 AGAGTAC 34905 0.0 19.948072 10-11 TACATGG 18315 0.0 19.762087 2 AGTACTT 27890 0.0 18.629326 12-13 ACATGGG 18595 0.0 18.554483 3 >>END_MODULE