FastQCFastQC Report
Wed 25 May 2016
SRR2049473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6254066
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67950.041.804951
GTATCAA113150.034.6234741
ATCAACG119600.031.1616173
TCAACGC119600.031.043734
CAACGCA128750.029.021195
TATCAAC137850.027.5818392
AACGCAG138100.027.0912256
ACGCAGA157950.023.4191597
GTACATG194800.023.153141
CGCAGAG159500.023.0129248
TACATGG197200.021.8546522
ACATGGG199300.021.0582433
GTGGTAT42200.020.9520071
GCAGAGT186800.018.9702249
TGGTATC43050.018.5591132
CATGGGG130550.017.2079454
CAGAGTA174600.016.8505210-11
AGAGTAC159600.016.78514710-11
GTACCTG43700.016.0355951
GTACACG6900.015.6768571
Produced by FastQC (version 0.11.5)