##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049473_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6254066 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.701248435817593 31.0 31.0 34.0 28.0 34.0 2 30.88116994607988 31.0 31.0 34.0 28.0 34.0 3 30.96007445396323 31.0 31.0 34.0 28.0 34.0 4 34.65960448770448 37.0 35.0 37.0 32.0 37.0 5 34.69285469644868 37.0 35.0 37.0 32.0 37.0 6 34.81515833059645 37.0 35.0 37.0 32.0 37.0 7 34.75226868408488 37.0 35.0 37.0 32.0 37.0 8 34.69411275800415 37.0 35.0 37.0 32.0 37.0 9 36.299638187380815 39.0 37.0 39.0 32.0 39.0 10-11 36.23848716978682 39.0 37.0 39.0 32.0 39.0 12-13 36.16424043174472 39.0 37.0 39.0 32.0 39.0 14-15 37.392500015190116 40.0 38.0 41.0 32.0 41.0 16-17 37.377359305130454 40.0 38.0 41.0 32.0 41.0 18-19 37.38625863558204 40.0 38.0 41.0 32.0 41.0 20-21 37.434383967166326 40.0 38.0 41.0 32.0 41.0 22-23 37.39681960503775 40.0 38.0 41.0 32.0 41.0 24-25 37.397402665721785 40.0 38.0 41.0 32.0 41.0 26-27 37.396557295685724 40.0 38.0 41.0 32.0 41.0 28-29 37.37994442335594 40.0 38.0 41.0 32.0 41.0 30-31 37.33253750440114 40.0 38.0 41.0 31.5 41.0 32-33 37.29664133701179 40.0 38.0 41.0 31.0 41.0 34-35 37.2417862235544 40.0 37.5 41.0 31.0 41.0 36-37 37.13982623464479 40.0 37.0 41.0 31.0 41.0 38-39 37.03976876803027 40.0 37.0 41.0 30.5 41.0 40-41 36.926681138318656 40.0 37.0 41.0 30.0 41.0 42-43 36.83423328119659 40.0 37.0 41.0 30.0 41.0 44-45 36.68517625173767 40.0 37.0 41.0 30.0 41.0 46-47 36.56776287618327 40.0 36.0 41.0 30.0 41.0 48-49 36.47180274400686 40.0 36.0 41.0 30.0 41.0 50-51 36.363545731688795 40.0 36.0 41.0 30.0 41.0 52-53 36.25567151034223 40.0 36.0 41.0 30.0 41.0 54-55 36.11600101438009 39.0 35.0 41.0 29.0 41.0 56-57 35.955872867347416 39.0 35.0 41.0 29.0 41.0 58-59 35.77707326721528 39.0 35.0 41.0 28.5 41.0 60-61 35.58540004214858 39.0 35.0 41.0 28.0 41.0 62-63 35.34143587547685 38.5 35.0 40.0 28.0 41.0 64-65 35.106078829356775 38.0 34.5 40.0 28.0 41.0 66-67 34.884255698612705 37.5 34.0 40.0 28.0 41.0 68-69 34.60708529459075 37.0 34.0 40.0 28.0 41.0 70-71 34.298875643461386 36.5 34.0 39.5 27.0 41.0 72-73 33.967856431320044 36.0 34.0 39.0 26.0 41.0 74-75 33.671476604180384 36.0 34.0 39.0 26.0 40.5 76-77 33.34921289605834 35.0 33.5 38.5 26.0 40.0 78-79 33.0039223282901 35.0 33.0 37.0 26.0 39.0 80-81 32.675221048194885 35.0 33.0 37.0 26.0 39.0 82-83 32.377437094523785 35.0 33.0 36.5 25.5 39.0 84-85 32.091019746193915 35.0 33.0 36.0 25.0 37.5 86-87 31.821369170072717 35.0 33.0 36.0 25.0 37.0 88-89 31.595283132605253 35.0 33.0 35.0 24.5 37.0 90-91 31.38868481720532 35.0 32.5 35.0 24.0 36.0 92-93 31.186555274600558 35.0 32.0 35.0 23.5 36.0 94-95 30.98960164475399 35.0 32.0 35.0 22.0 36.0 96-97 30.804550991307096 35.0 32.0 35.0 20.0 36.0 98-99 30.63386978966963 35.0 32.0 35.0 18.5 35.0 100 30.502435695433977 35.0 32.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2437932476488598 1101 2 0.21220672334889912 1101 3 0.2583251791479455 1101 4 0.3504548431942851 1101 5 0.3306352188408326 1101 6 0.4236095107269833 1101 7 0.5208903311113033 1101 8 0.4990822159944983 1101 9 0.6225982224557498 1101 10-11 0.6104040127900348 1101 12-13 0.6579195068068486 1101 14-15 0.7512101773405462 1101 16-17 0.6668892234195383 1101 18-19 0.6392018497261063 1101 20-21 0.644234403713881 1101 22-23 0.6313383738551579 1101 24-25 0.6906952005747726 1101 26-27 0.6552120754560846 1101 28-29 0.6108844320612832 1101 30-31 0.6202857502623118 1101 32-33 0.6465626707308729 1101 34-35 0.6349141823106876 1101 36-37 0.6176079904864764 1101 38-39 0.6032137247018667 1101 40-41 0.6770039597347761 1101 42-43 0.5789890753253388 1101 44-45 0.556866343731329 1101 46-47 0.5087421564540122 1101 48-49 0.5071320707928493 1101 50-51 0.42969561886692986 1101 52-53 0.4287281384125663 1101 54-55 0.4472121837986691 1101 56-57 0.424774851542324 1101 58-59 0.38951368428239164 1101 60-61 0.3760218045983734 1101 62-63 0.4119923117637967 1101 64-65 0.41279023593342856 1101 66-67 0.41400805457762146 1101 68-69 0.3673594400703877 1101 70-71 0.43172448714177136 1101 72-73 0.40730097190766656 1101 74-75 0.31520733983926164 1101 76-77 0.2771175608356202 1101 78-79 0.2756966725933978 1101 80-81 0.32424932450638977 1101 82-83 0.3094744905901621 1101 84-85 0.3189462351677932 1101 86-87 0.33133580649577254 1101 88-89 0.2591610219970093 1101 90-91 0.3095182423824383 1101 92-93 0.24311110912914558 1101 94-95 0.23200220204229893 1101 96-97 0.24863517384196854 1101 98-99 0.29354855823372716 1101 100 0.2926095938030677 1102 1 0.06673282949811465 1102 2 0.10690351871139114 1102 3 0.1085943516418304 1102 4 0.19757160704877919 1102 5 0.1807928910358001 1102 6 0.20222018314667878 1102 7 0.2665911097205509 1102 8 0.32677781021757113 1102 9 0.4105454570447833 1102 10-11 0.40900759815558985 1102 12-13 0.38803286539744875 1102 14-15 0.5024154822813429 1102 16-17 0.435747082208934 1102 18-19 0.40924263295401175 1102 20-21 0.40611775092599345 1102 22-23 0.42687102834052837 1102 24-25 0.3926407576788833 1102 26-27 0.3898542278377093 1102 28-29 0.4580569530602361 1102 30-31 0.36920710378528554 1102 32-33 0.3693213884628861 1102 34-35 0.398628715580017 1102 36-37 0.4349944237596617 1102 38-39 0.407726783940042 1102 40-41 0.35786180945864743 1102 42-43 0.38690444657047607 1102 44-45 0.3605742574923312 1102 46-47 0.3958361633353249 1102 48-49 0.2744725392219749 1102 50-51 0.33961705256658803 1102 52-53 0.2865086607485168 1102 54-55 0.31623952584041604 1102 56-57 0.28128313028562957 1102 58-59 0.27298782766385443 1102 60-61 0.2893407447400378 1102 62-63 0.25713649962744967 1102 64-65 0.31798817701395166 1102 66-67 0.33349249258442626 1102 68-69 0.34966945597715693 1102 70-71 0.2622427037374422 1102 72-73 0.2646593180177135 1102 74-75 0.3127508427581134 1102 76-77 0.23649648402504653 1102 78-79 0.2438369241327507 1102 80-81 0.2523012454250093 1102 82-83 0.23671730686163528 1102 84-85 0.20666124415308218 1102 86-87 0.20638892827400213 1102 88-89 0.19134672206548586 1102 90-91 0.14350537737442437 1102 92-93 0.14123317408838076 1102 94-95 0.18951907723480943 1102 96-97 0.2055143988306689 1102 98-99 0.21370529967400387 1102 100 0.1644339005576505 1103 1 0.21589065776868566 1103 2 0.26045642751170206 1103 3 0.22709207416922084 1103 4 0.31842559606221954 1103 5 0.34983613683063197 1103 6 0.3538898284016625 1103 7 0.3523424563768174 1103 8 0.3839665860866788 1103 9 0.46058741852937857 1103 10-11 0.5256882679622734 1103 12-13 0.4992116253963417 1103 14-15 0.6033156259318702 1103 16-17 0.5932970875597192 1103 18-19 0.5870642956258578 1103 20-21 0.5768936830695495 1103 22-23 0.5835858185924252 1103 24-25 0.5615758491402971 1103 26-27 0.5839419427397701 1103 28-29 0.567585595503445 1103 30-31 0.5750977220649602 1103 32-33 0.5860261817790615 1103 34-35 0.5671975203709749 1103 36-37 0.5515460273978121 1103 38-39 0.5304329983022953 1103 40-41 0.6148203274482782 1103 42-43 0.5901143353638574 1103 44-45 0.5575718332773576 1103 46-47 0.4480980149830529 1103 48-49 0.4626480169615306 1103 50-51 0.4902438623540988 1103 52-53 0.4189414549639565 1103 54-55 0.4527951872134537 1103 56-57 0.4406049492869215 1103 58-59 0.48034910520081553 1103 60-61 0.45241488995829826 1103 62-63 0.4829893519717956 1103 64-65 0.549677228931273 1103 66-67 0.4845386775406766 1103 68-69 0.4166281333521269 1103 70-71 0.43181929120637363 1103 72-73 0.4405738692635168 1103 74-75 0.4854630670259823 1103 76-77 0.3975526071961184 1103 78-79 0.4070747089546529 1103 80-81 0.4180199887926932 1103 82-83 0.4254337360261786 1103 84-85 0.3783638282447228 1103 86-87 0.3986656423044792 1103 88-89 0.4210368661917734 1103 90-91 0.3916486281185918 1103 92-93 0.35984963269726933 1103 94-95 0.45109845167014484 1103 96-97 0.4163671139598222 1103 98-99 0.41453201821640207 1103 100 0.3818604218217523 1104 1 0.02926142609886284 1104 2 0.06863099234008274 1104 3 0.09248783344010292 1104 4 0.20837287858135767 1104 5 0.22084413090836108 1104 6 0.26964652907638254 1104 7 0.2717761184684804 1104 8 0.20894053083642916 1104 9 0.3519719023957606 1104 10-11 0.36505915753715357 1104 12-13 0.38167426103727564 1104 14-15 0.4402509121879419 1104 16-17 0.4252605083396048 1104 18-19 0.4128430632912341 1104 20-21 0.4085055105991273 1104 22-23 0.4364070941069187 1104 24-25 0.4047204612788349 1104 26-27 0.41598031525133905 1104 28-29 0.39955581516979066 1104 30-31 0.3794491241896054 1104 32-33 0.3901085044118773 1104 34-35 0.397151233198727 1104 36-37 0.42833240012856066 1104 38-39 0.40716296171898847 1104 40-41 0.43053473753551685 1104 42-43 0.42574287942533573 1104 44-45 0.3451917055925051 1104 46-47 0.34557224505898887 1104 48-49 0.30841696143428265 1104 50-51 0.30470523388148507 1104 52-53 0.31199498346745713 1104 54-55 0.28457144940716717 1104 56-57 0.2564100027844489 1104 58-59 0.34704781619249303 1104 60-61 0.37155050476078344 1104 62-63 0.36180930444064074 1104 64-65 0.3512854065057738 1104 66-67 0.3712896382901931 1104 68-69 0.336522252867816 1104 70-71 0.37509137552851257 1104 72-73 0.31766953456597236 1104 74-75 0.2886278679470138 1104 76-77 0.32877206960021965 1104 78-79 0.29617781322276926 1104 80-81 0.2762783867923915 1104 82-83 0.3163597554630684 1104 84-85 0.2551543509451335 1104 86-87 0.2337403672384326 1104 88-89 0.19282891189320495 1104 90-91 0.18283546242687976 1104 92-93 0.2105893263169918 1104 94-95 0.24972334976252952 1104 96-97 0.2586667153306408 1104 98-99 0.3033783036933464 1104 100 0.2955182848008029 1105 1 0.18080623757029812 1105 2 0.1313499367076929 1105 3 0.13670603665832104 1105 4 0.23919357631812943 1105 5 0.21570797585895463 1105 6 0.2571061223644975 1105 7 0.29340548105464137 1105 8 0.23712094787183702 1105 9 0.33236973970456773 1105 10-11 0.3446479278924315 1105 12-13 0.36537090688881335 1105 14-15 0.39847467478253407 1105 16-17 0.3152510009783924 1105 18-19 0.3458944112350437 1105 20-21 0.3323649442550689 1105 22-23 0.34341258476712966 1105 24-25 0.3523999143372052 1105 26-27 0.40619006072453345 1105 28-29 0.41697305145555674 1105 30-31 0.3940235402314798 1105 32-33 0.3959138800054376 1105 34-35 0.38001599408843134 1105 36-37 0.3707904857090085 1105 38-39 0.4362969008307118 1105 40-41 0.42059858918702986 1105 42-43 0.43739050031968674 1105 44-45 0.39428632384424134 1105 46-47 0.32424592366770355 1105 48-49 0.4115500855108962 1105 50-51 0.43726594404861174 1105 52-53 0.44330801859639735 1105 54-55 0.37069061181325935 1105 56-57 0.3594135951911994 1105 58-59 0.3447314378199522 1105 60-61 0.34783486418229614 1105 62-63 0.3748666673650689 1105 64-65 0.3616151792137785 1105 66-67 0.387574905067396 1105 68-69 0.3982860474785781 1105 70-71 0.37209329772777266 1105 72-73 0.3691155476582253 1105 74-75 0.4071977190704601 1105 76-77 0.3612561592973833 1105 78-79 0.3406918580452185 1105 80-81 0.38542191934341474 1105 82-83 0.34567406230429043 1105 84-85 0.3314669698267707 1105 86-87 0.3306489605527858 1105 88-89 0.34746830372251125 1105 90-91 0.38422293632424953 1105 92-93 0.32798223030290785 1105 94-95 0.342611364903302 1105 96-97 0.3543623208809592 1105 98-99 0.292595493238462 1105 100 0.2998257614799833 1106 1 0.11697263204431962 1106 2 0.23661512871374768 1106 3 0.18293080842167697 1106 4 0.25386578243716684 1106 5 0.27234340556646686 1106 6 0.2830911753959171 1106 7 0.3014875544576441 1106 8 0.2678088331608137 1106 9 0.3232111572419356 1106 10-11 0.3566522923756352 1106 12-13 0.34775484440933724 1106 14-15 0.4429078583342445 1106 16-17 0.43265799804632366 1106 18-19 0.44396146303208184 1106 20-21 0.4157689059503724 1106 22-23 0.3781315064391393 1106 24-25 0.4290596137790388 1106 26-27 0.4683595657783286 1106 28-29 0.4000596226279072 1106 30-31 0.39388700769359986 1106 32-33 0.37072057325597996 1106 34-35 0.408809813124833 1106 36-37 0.3818879251681153 1106 38-39 0.39032839835602573 1106 40-41 0.45137569986938075 1106 42-43 0.4382409428328913 1106 44-45 0.3840288933316458 1106 46-47 0.3990257175452925 1106 48-49 0.3742772427448742 1106 50-51 0.4288581213994931 1106 52-53 0.42844228702657006 1106 54-55 0.36683848320481616 1106 56-57 0.2907519486725363 1106 58-59 0.36102297147199636 1106 60-61 0.3764374664467667 1106 62-63 0.36519305959902226 1106 64-65 0.39198260325886736 1106 66-67 0.37672266036030067 1106 68-69 0.3308093051628589 1106 70-71 0.35011872256118437 1106 72-73 0.27910288292256524 1106 74-75 0.3220860234659497 1106 76-77 0.3137147699377749 1106 78-79 0.3117406808819112 1106 80-81 0.32218413694614156 1106 82-83 0.2865602925447064 1106 84-85 0.32678274350298153 1106 86-87 0.33643170257173693 1106 88-89 0.3144791938141154 1106 90-91 0.32518850440281355 1106 92-93 0.22726045301473619 1106 94-95 0.25954158002219785 1106 96-97 0.289646912792648 1106 98-99 0.3451919753317725 1106 100 0.3100773520439901 1107 1 0.05660083935883975 1107 2 0.03740790937300176 1107 3 0.1398119990257065 1107 4 0.11925592215722958 1107 5 0.13693084198135352 1107 6 0.18012580235096465 1107 7 0.11312632682982837 1107 8 0.09836339316498766 1107 9 0.14100577583953822 1107 10-11 0.15665110567146456 1107 12-13 0.15434706216948513 1107 14-15 0.18076046213444386 1107 16-17 0.1786693140835922 1107 18-19 0.1818411223033678 1107 20-21 0.2691174754580743 1107 22-23 0.21027012621777885 1107 24-25 0.1893980991729407 1107 26-27 0.2634499388362528 1107 28-29 0.21901496245240537 1107 30-31 0.23249949657516567 1107 32-33 0.21662302389003685 1107 34-35 0.2549397615211859 1107 36-37 0.17449677180203338 1107 38-39 0.19351719700350145 1107 40-41 0.26706817409947803 1107 42-43 0.23206361829998912 1107 44-45 0.21163404371075956 1107 46-47 0.14297095741020627 1107 48-49 0.24097670119606107 1107 50-51 0.24159757053557485 1107 52-53 0.21308381639276774 1107 54-55 0.14805739168539844 1107 56-57 0.11297269702893686 1107 58-59 0.19598233169798362 1107 60-61 0.18142178655575236 1107 62-63 0.21092269427646215 1107 64-65 0.23606905054026583 1107 66-67 0.24850733645286027 1107 68-69 0.1890185353617042 1107 70-71 0.24054231747836496 1107 72-73 0.20026166598130857 1107 74-75 0.20763146850959657 1107 76-77 0.18305362452468188 1107 78-79 0.20183504323791368 1107 80-81 0.19863024236125426 1107 82-83 0.13768399932692432 1107 84-85 0.20289508404653134 1107 86-87 0.1340840938100456 1107 88-89 0.1535171317083872 1107 90-91 0.15334663124529868 1107 92-93 0.11751989872148272 1107 94-95 0.17344832050532233 1107 96-97 0.21152673107537368 1107 98-99 0.276437276735777 1107 100 0.22021298541066514 1108 1 0.09304846465786554 1108 2 0.08404709901380869 1108 3 0.08997520989146679 1108 4 0.11219516043698974 1108 5 0.1337607042945237 1108 6 0.1834450216138208 1108 7 0.1556673621994733 1108 8 0.15743202700120662 1108 9 0.23701709726586273 1108 10-11 0.23794672595422384 1108 12-13 0.23887039033633073 1108 14-15 0.21821781799980045 1108 16-17 0.23737189121411717 1108 18-19 0.22521262553355825 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-0.8939831541840562 2215 34-35 -0.7730677562809376 2215 36-37 -0.8152364944753003 2215 38-39 -0.8239790627942583 2215 40-41 -0.7299978156327 2215 42-43 -0.7558951008869528 2215 44-45 -0.6886802391303632 2215 46-47 -0.6219540726887018 2215 48-49 -0.5963024051809285 2215 50-51 -0.6868435332967877 2215 52-53 -0.6573559882344 2215 54-55 -0.6449043434512731 2215 56-57 -0.6242679589698383 2215 58-59 -0.6783800832695945 2215 60-61 -0.5991174113200728 2215 62-63 -0.6167754281651838 2215 64-65 -0.6872062373204244 2215 66-67 -0.6092988735633682 2215 68-69 -0.655838497299321 2215 70-71 -0.5902062345933814 2215 72-73 -0.5679796825818002 2215 74-75 -0.5661881220366283 2215 76-77 -0.5681160265586414 2215 78-79 -0.5221907598293427 2215 80-81 -0.5890327105762267 2215 82-83 -0.5874398147489686 2215 84-85 -0.5924074169806417 2215 86-87 -0.5964621725919272 2215 88-89 -0.5670677958092689 2215 90-91 -0.5715448627101907 2215 92-93 -0.5723855121460986 2215 94-95 -0.6445197944317833 2215 96-97 -0.6109905310273795 2215 98-99 -0.5888795234195996 2215 100 -0.5970709452451182 2216 1 -0.26598713157584086 2216 2 -0.3962484581417449 2216 3 -0.3678762632164343 2216 4 -0.4980593839268721 2216 5 -0.5006077473166002 2216 6 -0.6019693696659729 2216 7 -0.5691360502130891 2216 8 -0.5656660037805352 2216 9 -0.7424420796117346 2216 10-11 -0.7406078181615143 2216 12-13 -0.770441877493937 2216 14-15 -0.8633993998851963 2216 16-17 -0.9041964220602949 2216 18-19 -0.9813144647731065 2216 20-21 -0.8937887565160736 2216 22-23 -0.895316117098389 2216 24-25 -0.946759750115433 2216 26-27 -0.9514632555723495 2216 28-29 -0.900768295691762 2216 30-31 -1.024287520367146 2216 32-33 -0.9951759864408416 2216 34-35 -0.9606512846923465 2216 36-37 -0.9687755389218111 2216 38-39 -0.8752278748706246 2216 40-41 -0.9011005324223902 2216 42-43 -0.863597266311281 2216 44-45 -0.9265729082014431 2216 46-47 -0.8402349511094584 2216 48-49 -0.9651699780921135 2216 50-51 -0.9094725116892661 2216 52-53 -0.9903531666125502 2216 54-55 -0.9440356037222841 2216 56-57 -0.8975073911155533 2216 58-59 -0.9058129636682111 2216 60-61 -0.9682633108622909 2216 62-63 -0.9691802293631753 2216 64-65 -0.9433048326666125 2216 66-67 -0.9711366030767579 2216 68-69 -0.8796199516538366 2216 70-71 -0.8399729958809345 2216 72-73 -0.8892734090522865 2216 74-75 -0.9532125033641634 2216 76-77 -0.9333423272945112 2216 78-79 -0.8931199078870335 2216 80-81 -0.901346410789202 2216 82-83 -0.9005672863073073 2216 84-85 -0.8000403164258607 2216 86-87 -0.9418503580780317 2216 88-89 -0.928803615247972 2216 90-91 -0.9407519541279115 2216 92-93 -0.952815805133497 2216 94-95 -0.8858796106563958 2216 96-97 -0.8970999168148737 2216 98-99 -0.9250332353879855 2216 100 -0.9757617975370003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99687.0 3 18125.0 4 11546.0 5 9259.0 6 7976.0 7 7757.0 8 8226.0 9 9190.0 10 10490.0 11 11726.0 12 12930.0 13 14039.0 14 16307.0 15 17544.0 16 17741.0 17 18780.0 18 18067.0 19 16748.0 20 16732.0 21 18118.0 22 21205.0 23 25235.0 24 30753.0 25 37921.0 26 46330.0 27 53960.0 28 65053.0 29 82039.0 30 99325.0 31 117790.0 32 150250.0 33 201179.0 34 280305.0 35 411471.0 36 685522.0 37 1238982.0 38 1705026.0 39 639397.0 40 1335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.85316295594338 20.700087979458445 14.746528587883287 22.70022047671489 2 15.16762267758431 20.730050225155246 40.748021705864204 23.354305391396238 3 18.055254365444142 26.034228203804528 30.581473897532245 25.32904353321908 4 12.5311831561064 17.697576477090134 39.18328275798545 30.587957608818023 5 13.009377770443232 38.117045269921924 35.50049955928765 13.373077400347197 6 29.768992666796834 38.40899282030814 18.38040453747443 13.4416099754206 7 26.30151292221085 32.99654554759468 23.026899948822926 17.675041581371545 8 24.414938417558076 36.400811259382166 21.052062633215677 18.13218768984408 9 24.616732789955016 17.61155006740258 22.08377109029067 35.687946052351734 10-11 23.263267934305553 27.575375568524095 29.39881050710726 19.762545990063092 12-13 23.763908935517808 25.27963797967082 29.268920466144195 21.68753261866718 14-15 21.339746653183962 27.4703209519683 26.46716964821062 24.722762746637112 16-17 20.854883678310763 30.754466833304456 27.102313528564633 21.28833595982015 18-19 20.83657183574694 29.726945574050255 28.98133511238086 20.45514747782194 20-21 21.927457654873667 28.91273636878568 28.515615380014708 20.64419059632595 22-23 21.67789219415718 28.756359211016065 28.52135275635441 21.044395838472344 24-25 21.45156353399078 28.921220877142424 28.447981937855243 21.17923365101155 26-27 21.216164366191226 28.918335074024064 28.50426308658166 21.36123747320305 28-29 21.201293519640586 28.91753924231681 28.66854374775919 21.212623490283413 30-31 21.46371959875745 29.14475100802768 28.305242991260226 21.08628640195464 32-33 21.204816464136304 28.964925884744712 28.644599068555955 21.185658582563033 34-35 21.428835279674974 28.73853427034056 28.573179583297552 21.25945086668691 36-37 21.563544126315236 28.945400239292525 28.264262483621 21.22679315077124 38-39 21.400138192575135 29.06885855667683 28.38886664576426 21.142136604983776 40-41 21.399969791773525 28.79205394111411 28.478177834661857 21.329798432450513 42-43 21.305561672062787 28.836477218049406 28.45376776045807 21.404193349429743 44-45 21.428826171141267 28.916410555188698 28.351744519384965 21.303018754285073 46-47 21.470479133700515 28.95643594298779 28.2447468923226 21.328338030989094 48-49 21.416970584117227 28.83373982267357 28.290506620774593 21.45878297243461 50-51 21.375650114101536 28.79263775838555 28.2872504276259 21.544461699887012 52-53 21.436232383031548 28.863331839216865 28.219156023019092 21.481279754732494 54-55 21.49726602584758 29.038702609209043 28.139360800313305 21.32467056463007 56-57 21.44561813600146 28.834293426514307 28.18295098730908 21.537137450175152 58-59 21.419031150942384 28.605081663771426 28.365104791606438 21.610782393679756 60-61 21.512708860727106 28.888618112778996 28.13296087759603 21.465712148897865 62-63 21.375580120914144 28.929231985117177 28.167067786780493 21.528120107188187 64-65 21.444239406984327 28.73719155616715 28.220407592719475 21.59816144412905 66-67 21.320643740921735 28.758706857110234 28.209129604537537 21.711519797430494 68-69 21.579538097404985 28.88204546103304 28.131381980894105 21.407034460667866 70-71 21.575957864463426 28.663750654839166 28.17911169622916 21.58117978446825 72-73 21.407017607831133 28.829436990167263 28.257459954650617 21.50608544735099 74-75 21.41242948243955 28.846251187370626 28.165786565717543 21.57553276447229 76-77 21.499685055008634 28.81521995857342 28.004462480288954 21.680632506128994 78-79 21.542643781057293 28.81797823314882 28.194537594337255 21.444840391456626 80-81 21.438285308846734 28.759828842423385 28.21761874554652 21.584267103183365 82-83 21.394858937658345 28.79706375786605 28.301101546559664 21.506975757915942 84-85 21.37392012837924 28.73098768299699 28.196913733004603 21.69817845561917 86-87 21.522494503662426 28.857261542508812 28.096350127997997 21.52389382583077 88-89 21.392567756445892 28.747953825379025 28.185475694420013 21.674002723755073 90-91 21.319147530911238 28.760384938270285 28.181912462229775 21.73855506858871 92-93 21.392144745724416 28.861131008667947 28.111318042148635 21.635406203459006 94-95 21.287787790749277 28.681779357507008 28.28108280822138 21.749350043522337 96-97 21.341969836206932 28.78528848831695 28.20896191893288 21.663779756543246 98-99 21.28012690610012 28.808404330894543 28.161816471487505 21.749652291517833 100 21.36083448840061 28.709796782783176 28.113164824635522 21.816203904180693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3954.0 1 2883.5 2 2095.5 3 2373.0 4 2157.0 5 1855.5 6 1700.0 7 1631.5 8 1704.5 9 1701.0 10 1457.5 11 1276.5 12 1294.0 13 1341.0 14 1424.5 15 1567.5 16 1825.0 17 2290.5 18 2983.5 19 4057.0 20 5758.5 21 8426.0 22 12552.0 23 18943.0 24 27486.5 25 38193.5 26 52165.0 27 68371.0 28 87517.0 29 108461.5 30 126578.0 31 143474.5 32 160043.0 33 175016.0 34 188031.0 35 198280.0 36 206984.5 37 215349.5 38 222597.0 39 225674.0 40 226693.0 41 226324.5 42 222724.0 43 218446.0 44 213722.0 45 209839.5 46 205081.0 47 196843.5 48 190088.0 49 186103.0 50 180716.0 51 171526.5 52 158892.0 53 148417.5 54 142292.0 55 135988.5 56 125794.5 57 113646.0 58 102632.5 59 93108.5 60 82497.0 61 70419.0 62 60040.0 63 50293.0 64 41347.5 65 33939.0 66 27425.5 67 21724.5 68 17126.0 69 13289.5 70 9909.0 71 7463.0 72 5721.5 73 4208.5 74 3005.0 75 2178.5 76 1460.5 77 1021.0 78 736.5 79 476.5 80 324.5 81 235.5 82 171.5 83 131.5 84 103.0 85 79.5 86 66.5 87 52.0 88 40.0 89 36.5 90 26.0 91 16.5 92 14.0 93 14.5 94 13.0 95 10.5 96 9.5 97 10.0 98 9.5 99 9.5 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0780292372993825 2 0.013191418190981675 3 0.017492620001132064 4 0.011032822487002856 5 0.008858237185216786 6 0.007275267002298985 7 0.020242830823979152 8 0.009257977130398047 9 0.009529800293121308 10-11 0.012999543017294668 12-13 0.014974258346490108 14-15 0.009953524635013445 16-17 0.012511860284173528 18-19 0.0085144608323609 20-21 0.012919595028258417 22-23 0.013327329772343303 24-25 0.015797722633563507 26-27 0.0051566452928382914 28-29 0.011832302377365381 30-31 0.010257326993351204 32-33 0.012232042322546646 34-35 0.008618393218108028 36-37 0.010105425814182325 38-39 0.01914754337418249 40-41 0.012831652240318538 42-43 0.007946830110203506 44-45 0.020066945248099398 46-47 0.017980302734253203 48-49 0.01814019871232571 50-51 0.010305295786772957 52-53 0.010377248976905585 54-55 0.011432562432184119 56-57 0.020586607176835036 58-59 0.0020786477149425673 60-61 0.006987454241768475 62-63 0.015214102313598866 64-65 0.0247838766012383 66-67 0.012871626234836666 68-69 0.01382300730436807 70-71 0.02520760094313044 72-73 0.02046668519328066 74-75 0.020458690394377036 76-77 0.009481831499699556 78-79 0.011256676856304363 80-81 0.01969118969962901 82-83 0.012264021518161144 84-85 0.011488526024509494 86-87 0.01660519732282966 88-89 0.014934284351971982 90-91 0.015342019096056867 92-93 0.008098731289372385 94-95 0.015270065905924243 96-97 0.013439256956994057 98-99 0.009969514232820696 100 0.0073871941869497375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6254066.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.68437630790034 #Duplication Level Percentage of deduplicated Percentage of total 1 75.621974288478 46.646943191568404 2 14.69359317482187 18.127302614218163 3 4.570679362487088 8.458185173352224 4 1.884921504826943 4.650808296583957 5 0.9431072356205182 2.9087490810359835 6 0.5603702734585109 2.073965449186548 7 0.35072972714881573 1.514418113027033 8 0.24989106654430618 1.2331499667761237 9 0.17446102190138796 0.9685367393423452 >10 0.8885686289554856 9.556246514360833 >50 0.044903763916271364 1.883896279037195 >100 0.01620260055370023 1.6674251214804499 >500 4.675020080412526E-4 0.1636683123067198 >1k 1.2984927904637113E-4 0.14670514772402615 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.5989597807250515E-5 0.0 0.0 0.0 3 0.0 1.5989597807250515E-5 0.0 0.0 0.0 4 0.0 1.5989597807250515E-5 0.0 0.0 0.0 5 0.0 1.5989597807250515E-5 0.0 3.197919561450103E-5 0.0 6 0.0 1.5989597807250515E-5 0.0 3.197919561450103E-5 0.0 7 0.0 1.5989597807250515E-5 0.0 3.197919561450103E-5 0.0 8 0.0 1.5989597807250515E-5 0.0 3.197919561450103E-5 0.0 9 0.0 1.5989597807250515E-5 0.0 3.197919561450103E-5 0.0 10-11 0.0 3.197919561450103E-5 0.0 3.197919561450103E-5 0.0 12-13 0.0 3.197919561450103E-5 0.0 3.197919561450103E-5 0.0 14-15 0.0 7.994798903625258E-5 0.0 4.796879342175154E-5 0.0 16-17 0.0 1.1992198355437886E-4 0.0 7.994798903625258E-5 0.0 18-19 0.0 1.5989597807250516E-4 0.0 7.994798903625258E-5 0.0 20-21 0.0 1.7588557587975567E-4 0.0 9.593758684350309E-5 0.0 22-23 0.0 1.7588557587975567E-4 0.0 1.8388037478338093E-4 0.0 24-25 0.0 2.078647714942567E-4 0.0 2.958075594341345E-4 0.0 26-27 0.0 2.5583356491600825E-4 0.0 4.3171914079576394E-4 0.0 28-29 0.0 2.638283638196335E-4 0.0 0.0011752354388329129 0.0 30-31 0.0 2.718231627232588E-4 0.0 0.002790184817365215 0.0 32-33 0.0 2.8781276053050926E-4 0.0 0.006563729899876336 0.0 34-35 0.0 3.038023583377598E-4 0.0 0.012695740658956909 0.0 36-37 0.0 3.3578155395226086E-4 0.0 0.021737858218957073 0.0 38-39 0.0 3.7575554847038713E-4 0.0 0.03249885754323667 0.0 40-41 0.0 4.636983364102649E-4 0.0 0.043163919280672765 0.0 42-43 0.0 5.436463254465176E-4 0.0 0.05461247131066413 0.0 44-45 0.0 5.436463254465176E-4 0.0 0.06975462043413037 0.0 46-47 0.0 5.516411243501428E-4 0.0 0.08783885555413071 0.0 48-49 0.0 5.59635923253768E-4 0.0 0.10647473179848119 0.0 50-51 0.0 5.91615118868269E-4 0.0 0.1245829513151924 0.0 52-53 0.0 5.91615118868269E-4 0.0 0.14258723844615648 0.0 54-55 0.0 5.91615118868269E-4 0.0 0.16435707586072806 0.0 56-57 0.0 5.91615118868269E-4 0.0 0.18942077042359323 0.0 58-59 0.0 5.91615118868269E-4 0.0 0.21657910229920824 0.0 60-61 0.0 6.076047166755196E-4 0.0 0.24532839915664467 0.0 62-63 0.0 6.076047166755196E-4 0.0 0.2739178000360086 0.0 64-65 0.0 6.076047166755196E-4 0.0 0.3077038202027289 0.0 66-67 0.0 6.235943144827701E-4 0.0 0.34727807477567396 0.0 68-69 0.0 6.235943144827701E-4 0.0 0.3925446261680001 0.0 70-71 0.0 6.235943144827701E-4 0.0 0.44169664982748824 0.0 72-73 0.0 6.795579068081469E-4 0.0 0.49367883229885967 0.0 74-75 0.0 7.035423035190227E-4 0.0 0.5480834388380296 0.0 76-77 0.0 7.035423035190227E-4 0.0 0.6085720873428582 0.0 78-79 0.0 7.035423035190227E-4 0.0 0.678382671369314 0.0 80-81 0.0 7.035423035190227E-4 0.0 0.7570355029831792 0.0 82-83 0.0 7.035423035190227E-4 0.0 0.8385264882078315 0.0 84-85 0.0 7.195319013262732E-4 0.0 0.9239988832864892 0.0 86-87 0.0 7.195319013262732E-4 0.0 1.0175460252578084 0.0 88 0.0 7.195319013262732E-4 0.0 1.0955113041659619 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6795 0.0 41.80495 1 GTATCAA 11315 0.0 34.623474 1 ATCAACG 11960 0.0 31.161617 3 TCAACGC 11960 0.0 31.04373 4 CAACGCA 12875 0.0 29.02119 5 TATCAAC 13785 0.0 27.581839 2 AACGCAG 13810 0.0 27.091225 6 ACGCAGA 15795 0.0 23.419159 7 GTACATG 19480 0.0 23.15314 1 CGCAGAG 15950 0.0 23.012924 8 TACATGG 19720 0.0 21.854652 2 ACATGGG 19930 0.0 21.058243 3 GTGGTAT 4220 0.0 20.952007 1 GCAGAGT 18680 0.0 18.970224 9 TGGTATC 4305 0.0 18.559113 2 CATGGGG 13055 0.0 17.207945 4 CAGAGTA 17460 0.0 16.85052 10-11 AGAGTAC 15960 0.0 16.785147 10-11 GTACCTG 4370 0.0 16.035595 1 GTACACG 690 0.0 15.676857 1 >>END_MODULE