FastQCFastQC Report
Wed 25 May 2016
SRR2049473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6254066
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222320.3554807384507935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208530.333431083074595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121260.19388986301071975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120950.058.246641
GTATCAA227700.041.418051
ATCAACG275650.033.710763
TCAACGC276800.033.672594
TATCAAC286100.032.5944562
CAACGCA287750.032.374885
GTGGTAT50200.032.342261
AACGCAG300500.031.0772086
TGGTATC52050.029.7997822
ACGCAGA341450.027.1711887
CGCAGAG343700.026.911278
GCAGAGT370550.024.669569
GTACATG193150.022.5373041
GAGTACT264700.021.81272112-13
CAGAGTA362050.021.69184710-11
TACATGG194350.021.54832
AGAGTAC347300.020.72530210-11
ACATGGG195200.020.5153833
GTACTTT289900.019.76260214-15
AGTACTT273600.019.3080712-13
Produced by FastQC (version 0.11.5)