##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049473_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6254066 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99097786943726 33.0 31.0 34.0 30.0 34.0 2 32.08769287052615 33.0 31.0 34.0 30.0 34.0 3 32.116871967772646 34.0 31.0 34.0 30.0 34.0 4 35.81936135627606 37.0 35.0 37.0 35.0 37.0 5 35.871692431771585 37.0 35.0 37.0 35.0 37.0 6 35.94543389852298 37.0 35.0 37.0 35.0 37.0 7 35.91611457250371 37.0 35.0 37.0 35.0 37.0 8 35.915026640268906 37.0 35.0 37.0 35.0 37.0 9 37.612142244741264 39.0 37.0 39.0 35.0 39.0 10-11 37.59986415237703 39.0 37.0 39.0 35.0 39.0 12-13 37.58453812287878 39.0 37.0 39.0 35.0 39.0 14-15 38.87846970275018 40.0 38.0 41.0 35.0 41.0 16-17 38.885399354595876 40.0 38.0 41.0 35.0 41.0 18-19 38.96915038952259 40.0 38.5 41.0 35.0 41.0 20-21 38.99950752038754 40.0 39.0 41.0 35.0 41.0 22-23 38.97102724851321 40.0 39.0 41.0 35.0 41.0 24-25 38.95433163001478 40.0 38.0 41.0 35.0 41.0 26-27 38.85501799949025 40.0 38.0 41.0 35.0 41.0 28-29 38.77540859018757 40.0 38.0 41.0 35.0 41.0 30-31 38.66076509266132 40.0 38.0 41.0 35.0 41.0 32-33 38.52481233808534 40.0 38.0 41.0 34.5 41.0 34-35 38.41242833062523 40.0 38.0 41.0 34.0 41.0 36-37 38.284474852040255 40.0 38.0 41.0 34.0 41.0 38-39 38.182945942687525 40.0 38.0 41.0 34.0 41.0 40-41 38.094263795745036 40.0 38.0 41.0 34.0 41.0 42-43 37.95006472589192 40.0 38.0 41.0 33.0 41.0 44-45 37.769765461381446 40.0 37.5 41.0 33.0 41.0 46-47 37.509493903646046 40.0 37.0 41.0 33.0 41.0 48-49 37.25216459180316 40.0 37.0 41.0 32.0 41.0 50-51 37.09388092482555 40.0 37.0 41.0 32.0 41.0 52-53 36.983123938890316 40.0 37.0 41.0 31.5 41.0 54-55 36.84531255346522 40.0 36.0 41.0 31.0 41.0 56-57 36.7005950209032 40.0 36.0 41.0 31.0 41.0 58-59 36.52639019159695 39.0 35.5 41.0 31.0 41.0 60-61 36.345519378912854 39.0 35.0 41.0 31.0 41.0 62-63 36.13289098643986 39.0 35.0 41.0 30.5 41.0 64-65 35.919263324051904 38.5 35.0 40.5 30.0 41.0 66-67 35.66524529801892 38.0 35.0 40.0 30.0 41.0 68-69 35.37878006084362 37.0 35.0 40.0 30.0 41.0 70-71 35.09681109857171 37.0 35.0 40.0 29.0 41.0 72-73 34.76787525107666 36.5 34.5 39.0 29.0 41.0 74-75 34.44753788975045 36.0 34.0 39.0 29.0 41.0 76-77 34.12851519315594 35.5 34.0 39.0 29.0 40.0 78-79 33.818204029186774 35.0 34.0 37.5 29.0 39.5 80-81 33.50783858053305 35.0 34.0 37.0 29.0 39.0 82-83 33.227024227118804 35.0 34.0 36.5 29.0 39.0 84-85 32.93991724743551 35.0 34.0 36.0 28.5 37.5 86-87 32.68895955047485 35.0 34.0 36.0 28.0 37.0 88-89 32.467553588337566 35.0 34.0 35.5 27.0 37.0 90-91 32.27077648365079 35.0 33.0 35.0 27.0 36.0 92-93 32.106358247578456 35.0 33.0 35.0 27.0 36.0 94-95 31.944057753787696 35.0 33.0 35.0 27.0 36.0 96-97 31.79729794984575 35.0 33.0 35.0 26.0 36.0 98-99 31.66296726001932 35.0 33.0 35.0 26.0 35.5 100 31.554647328633884 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28822356390207204 1101 2 0.3114095991009762 1101 3 0.4399163688100103 1101 4 0.23774868988700604 1101 5 0.26745549143476666 1101 6 0.23480048588589852 1101 7 0.28405679899945824 1101 8 0.2661138555904117 1101 9 0.31562192806698164 1101 10-11 0.2961002235957224 1101 12-13 0.30602735324670505 1101 14-15 0.4358420066242772 1101 16-17 0.38606411820984476 1101 18-19 0.3935224220911948 1101 20-21 0.38980796898272985 1101 22-23 0.40423815811502095 1101 24-25 0.3583490308233621 1101 26-27 0.33896874086693174 1101 28-29 0.34812713737309053 1101 30-31 0.38744611623382497 1101 32-33 0.38038444535364135 1101 34-35 0.39293972572198754 1101 36-37 0.37192692463336385 1101 38-39 0.371576470561493 1101 40-41 0.39922603887449526 1101 42-43 0.35639358059373194 1101 44-45 0.3388586711509092 1101 46-47 0.3469167181980737 1101 48-49 0.35853513483514377 1101 50-51 0.3398935683092219 1101 52-53 0.36877877572737106 1101 54-55 0.36441715743006853 1101 56-57 0.4129250550746235 1101 58-59 0.35417960937068216 1101 60-61 0.2992447325156462 1101 62-63 0.2923779976757146 1101 64-65 0.29842836306615794 1101 66-67 0.4011920892506211 1101 68-69 0.38505478809884863 1101 70-71 0.3783162575559871 1101 72-73 0.38253876855408464 1101 74-75 0.34455774704694875 1101 76-77 0.337458789281861 1101 78-79 0.3046898502639763 1101 80-81 0.31231196031060904 1101 82-83 0.3173655988578261 1101 84-85 0.28047832114669546 1101 86-87 0.2651312284242451 1101 88-89 0.288788753040194 1101 90-91 0.28403583160557133 1101 92-93 0.2880828888703135 1101 94-95 0.2571819279219092 1101 96-97 0.23338231905967177 1101 98-99 0.30023437414966025 1101 100 0.2862518236867153 1102 1 0.23118080840595923 1102 2 0.2335762348567343 1102 3 0.3130328619020446 1102 4 0.22087570080084618 1102 5 0.18387493731633953 1102 6 0.16656156147849543 1102 7 0.15748193946849653 1102 8 0.15407411626831902 1102 9 0.20945894000782062 1102 10-11 0.23310145649243452 1102 12-13 0.21261606119873022 1102 14-15 0.26837804986534763 1102 16-17 0.26411568867896307 1102 18-19 0.2564736343869427 1102 20-21 0.2850781934546589 1102 22-23 0.22604569715527134 1102 24-25 0.2593132901649966 1102 26-27 0.23797873712754836 1102 28-29 0.2401966947010905 1102 30-31 0.2120492839419228 1102 32-33 0.25621250819935426 1102 34-35 0.23254965850437515 1102 36-37 0.27891110190154933 1102 38-39 0.2009904284878914 1102 40-41 0.21526439576076228 1102 42-43 0.21309914631512328 1102 44-45 0.25231345419126683 1102 46-47 0.26551289613850315 1102 48-49 0.22648178436238453 1102 50-51 0.2193165994500248 1102 52-53 0.16959661433142514 1102 54-55 0.19606831918973455 1102 56-57 0.18854068536839463 1102 58-59 0.1880967453983473 1102 60-61 0.12905692278317815 1102 62-63 0.12984032557895375 1102 64-65 0.12558467776947424 1102 66-67 0.157780438359147 1102 68-69 0.19531979381117992 1102 70-71 0.14856109892190972 1102 72-73 0.19367670828793848 1102 74-75 0.21156903217826795 1102 76-77 0.16283939527242097 1102 78-79 0.18409489026495862 1102 80-81 0.18109260968974183 1102 82-83 0.2038171709553822 1102 84-85 0.12857308392169386 1102 86-87 0.17569327121268685 1102 88-89 0.16985847711501378 1102 90-91 0.21238117878133522 1102 92-93 0.1813627140915557 1102 94-95 0.08908812038409408 1102 96-97 0.12586404276867214 1102 98-99 0.173087043824264 1102 100 0.18319506327739532 1103 1 0.25380022367269817 1103 2 0.26212059017721856 1103 3 0.3493065670250388 1103 4 0.19142512601298733 1103 5 0.21875575843122874 1103 6 0.20518697922086204 1103 7 0.21850507107392758 1103 8 0.1996240477985367 1103 9 0.23321166888246125 1103 10-11 0.2512993068995897 1103 12-13 0.2456581388069239 1103 14-15 0.3198437537966683 1103 16-17 0.3108483073188708 1103 18-19 0.29807368577372984 1103 20-21 0.30923070388750773 1103 22-23 0.3043917375741927 1103 24-25 0.31977947189280087 1103 26-27 0.34749323861636583 1103 28-29 0.34190814565049976 1103 30-31 0.33041919860605873 1103 32-33 0.37489364369881173 1103 34-35 0.34995361416073933 1103 36-37 0.30150091703493587 1103 38-39 0.3656682538056302 1103 40-41 0.38302806494056085 1103 42-43 0.37723853681841746 1103 44-45 0.3874600533456558 1103 46-47 0.42978225159979644 1103 48-49 0.4000904612519989 1103 50-51 0.3536125243265644 1103 52-53 0.3801745395586167 1103 54-55 0.36259237792646815 1103 56-57 0.36167791721904763 1103 58-59 0.33238649019845923 1103 60-61 0.33089424021056857 1103 62-63 0.36047833308492727 1103 64-65 0.3329435585345024 1103 66-67 0.3012612127130154 1103 68-69 0.29047289780459096 1103 70-71 0.3144430025388445 1103 72-73 0.3573297338469885 1103 74-75 0.3891087745516444 1103 76-77 0.36488025855611284 1103 78-79 0.3433988533153567 1103 80-81 0.2779574282774817 1103 82-83 0.27068836615484315 1103 84-85 0.269092946150451 1103 86-87 0.2819778828463555 1103 88-89 0.2865947520305028 1103 90-91 0.26821787568447775 1103 92-93 0.3225403104576756 1103 94-95 0.32855991019870245 1103 96-97 0.3801249939491811 1103 98-99 0.3725664170252614 1103 100 0.34404614260970945 1104 1 0.23427889252590717 1104 2 0.24987992039380913 1104 3 0.3114111758274518 1104 4 0.21098258333944386 1104 5 0.16396802294630675 1104 6 0.15513646563878325 1104 7 0.14913381241663615 1104 8 0.13693934892083348 1104 9 0.1755463141612239 1104 10-11 0.20484811624428545 1104 12-13 0.21831647364635387 1104 14-15 0.32240810708608336 1104 16-17 0.3053659703465854 1104 18-19 0.21806831906828705 1104 20-21 0.300792402810913 1104 22-23 0.25446736919452917 1104 24-25 0.25695552828838686 1104 26-27 0.2453538447430219 1104 28-29 0.2673066449104695 1104 30-31 0.28695543109558486 1104 32-33 0.2999231740756301 1104 34-35 0.315700849372746 1104 36-37 0.3185022327140743 1104 38-39 0.3243748069028882 1104 40-41 0.33588321757820694 1104 42-43 0.3073193812547501 1104 44-45 0.2737294914755921 1104 46-47 0.26763729853304596 1104 48-49 0.3316495463252167 1104 50-51 0.3012293682991043 1104 52-53 0.3733204420980556 1104 54-55 0.3553127193673049 1104 56-57 0.38121554551113945 1104 58-59 0.3906587559083263 1104 60-61 0.3755974106663018 1104 62-63 0.34906787166985964 1104 64-65 0.32437617952617614 1104 66-67 0.28889569695781603 1104 68-69 0.31270814512916445 1104 70-71 0.28701944800732804 1104 72-73 0.3193340496136159 1104 74-75 0.3378376337989124 1104 76-77 0.34004399567216126 1104 78-79 0.34356002098211036 1104 80-81 0.2946107582787292 1104 82-83 0.2938730104900813 1104 84-85 0.2663016879089142 1104 86-87 0.27350099588164056 1104 88-89 0.2781139862947555 1104 90-91 0.2737598195918238 1104 92-93 0.32379003787151817 1104 94-95 0.2818564606732856 1104 96-97 0.29151063501379326 1104 98-99 0.35559746227976063 1104 100 0.2739110750986562 1105 1 0.199738487569725 1105 2 0.19147737751546146 1105 3 0.2660676174578782 1105 4 0.16953959370948724 1105 5 0.17471140939380092 1105 6 0.12012732526770975 1105 7 0.1506143676939331 1105 8 0.08840508559953264 1105 9 0.1677675862461001 1105 10-11 0.12698571994155827 1105 12-13 0.14049027647087087 1105 14-15 0.1734571234110689 1105 16-17 0.2119824514568549 1105 18-19 0.2282490949980982 1105 20-21 0.19369947339660598 1105 22-23 0.1967741353515322 1105 24-25 0.1991851472653252 1105 26-27 0.1809288473351245 1105 28-29 0.20754853676927354 1105 30-31 0.19766796110952356 1105 32-33 0.20713525823922652 1105 34-35 0.16199401485071974 1105 36-37 0.1507306656178855 1105 38-39 0.16511811824542377 1105 40-41 0.19013363765390068 1105 42-43 0.12189446665737336 1105 44-45 0.16747006860745728 1105 46-47 0.1232724601974553 1105 48-49 0.1583147317822835 1105 50-51 0.131173299764761 1105 52-53 0.10179042288972084 1105 54-55 0.10415279276922718 1105 56-57 0.09312635807948766 1105 58-59 0.13338103049810712 1105 60-61 0.11629154045293433 1105 62-63 0.08373232904774852 1105 64-65 0.08893317877677731 1105 66-67 0.08061576414456084 1105 68-69 0.0738907736642318 1105 70-71 0.11387905898900641 1105 72-73 0.11387392395813833 1105 74-75 0.13680230584041908 1105 76-77 0.08097706530255522 1105 78-79 0.08348280089170856 1105 80-81 0.09641406858344936 1105 82-83 0.025382766965662995 1105 84-85 0.08757695865497794 1105 86-87 0.10841995421682071 1105 88-89 0.1084824558575832 1105 90-91 0.07470299522847768 1105 92-93 0.06284391070750672 1105 94-95 0.0580773515273485 1105 96-97 0.09014426000146614 1105 98-99 0.19108979698959416 1105 100 0.15812929441079504 1106 1 0.1672082409388409 1106 2 0.12801543961185047 1106 3 0.24064163267547656 1106 4 0.1354918625514756 1106 5 0.17693556330959126 1106 6 0.11338411942769966 1106 7 0.10305288867377982 1106 8 0.09777675496675897 1106 9 0.1601146066265855 1106 10-11 0.15003844220213836 1106 12-13 0.15686428752691484 1106 14-15 0.202930916623977 1106 16-17 0.21785253156873097 1106 18-19 0.212013533454936 1106 20-21 0.22209456756915813 1106 22-23 0.20296017211279604 1106 24-25 0.18211679362268285 1106 26-27 0.1871545470805458 1106 28-29 0.2227653121592894 1106 30-31 0.2017917244811258 1106 32-33 0.20522703129830688 1106 34-35 0.19359458795858586 1106 36-37 0.22850995560998655 1106 38-39 0.15254213318468857 1106 40-41 0.17298909167388388 1106 42-43 0.19977218708377364 1106 44-45 0.1880945473668234 1106 46-47 0.19286429064113975 1106 48-49 0.22062499502781918 1106 50-51 0.20935595571806687 1106 52-53 0.19309352041150873 1106 54-55 0.2562580699022732 1106 56-57 0.2541832971390434 1106 58-59 0.20609533426637228 1106 60-61 0.2304515676500074 1106 62-63 0.19263052102221678 1106 64-65 0.18643328670307113 1106 66-67 0.21340585643913101 1106 68-69 0.23572155339101641 1106 70-71 0.23675724512755636 1106 72-73 0.27577245658440575 1106 74-75 0.2695871279374842 1106 76-77 0.2116910499389988 1106 78-79 0.2003102780207442 1106 80-81 0.1689085968068298 1106 82-83 0.18336224303610038 1106 84-85 0.21139078289818514 1106 86-87 0.22132567440728934 1106 88-89 0.22866485881767318 1106 90-91 0.2176018664174535 1106 92-93 0.22586565417739024 1106 94-95 0.19070486891166283 1106 96-97 0.1727091979448332 1106 98-99 0.19382597314580607 1106 100 0.17203105462946056 1107 1 0.061514174864470306 1107 2 0.0874888003694636 1107 3 0.16993869797683203 1107 4 0.04627412859664304 1107 5 0.09675864682301238 1107 6 0.06225983140262059 1107 7 0.04201214773583217 1107 8 0.05516736341766659 1107 9 0.04976122169480135 1107 10-11 0.05609997831421509 1107 12-13 0.06265481915688298 1107 14-15 0.0881046257225151 1107 16-17 0.1015305090484091 1107 18-19 0.08410094020221237 1107 20-21 0.07550735057344582 1107 22-23 0.07710801559281322 1107 24-25 0.10155082499754542 1107 26-27 0.09745259976820364 1107 28-29 0.1265853267104191 1107 30-31 0.11959507556069582 1107 32-33 0.0941146400689945 1107 34-35 0.10861100063077345 1107 36-37 0.11360901997851869 1107 38-39 0.07078989286539894 1107 40-41 0.11875100262879101 1107 42-43 0.04995327710165043 1107 44-45 0.10982156451393621 1107 46-47 0.10799978890345585 1107 48-49 0.048907518221049884 1107 50-51 0.007610383084696082 1107 52-53 0.06859699176337841 1107 54-55 0.10798149476093499 1107 56-57 0.055590084761661274 1107 58-59 0.011465177539427884 1107 60-61 0.05239627754598786 1107 62-63 0.05044981683887784 1107 64-65 0.03998942564654584 1107 66-67 0.054756691133285074 1107 68-69 0.11359697996878282 1107 70-71 0.046447262430255876 1107 72-73 0.06292940090905574 1107 74-75 0.05675029986417712 1107 76-77 0.045351124792702535 1107 78-79 0.06187390226508427 1107 80-81 0.06798867965191846 1107 82-83 0.09568201753048555 1107 84-85 0.06950061364891269 1107 86-87 0.042259748285538024 1107 88-89 0.04996101585786761 1107 90-91 0.10723751398652581 1107 92-93 0.10536402844457626 1107 94-95 0.020069074682567134 1107 96-97 0.06087206952811641 1107 98-99 0.11570573953184393 1107 100 0.06887681225375175 1108 1 0.05544761111578822 1108 2 0.07134744650553415 1108 3 0.14603171816589366 1108 4 0.11342192745786406 1108 5 0.10887401479018166 1108 6 0.10297977075119746 1108 7 0.06231749034861167 1108 8 0.029441715952422953 1108 9 0.08578542711752846 1108 10-11 0.030232679851430078 1108 12-13 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1 -0.3548127576140665 2215 2 -0.34931067568330576 2215 3 -0.2750485586104894 2215 4 -0.2431864275055986 2215 5 -0.22561934340733814 2215 6 -0.2470474536170002 2215 7 -0.2501609892010421 2215 8 -0.27423677281784364 2215 9 -0.38121568681064844 2215 10-11 -0.38662797200881727 2215 12-13 -0.4057171517368374 2215 14-15 -0.5241087163163911 2215 16-17 -0.54526707056106 2215 18-19 -0.4926180479043367 2215 20-21 -0.49024617437898144 2215 22-23 -0.5028034124400449 2215 24-25 -0.463821680998727 2215 26-27 -0.4354278292878391 2215 28-29 -0.4181527924416244 2215 30-31 -0.4775390606959391 2215 32-33 -0.5202050830169398 2215 34-35 -0.5224166748592012 2215 36-37 -0.48503790882733 2215 38-39 -0.5289714677983639 2215 40-41 -0.5758180893740459 2215 42-43 -0.4810073714672214 2215 44-45 -0.4543016580042547 2215 46-47 -0.500935499902937 2215 48-49 -0.4615219748107222 2215 50-51 -0.5007171782328825 2215 52-53 -0.5655544255662619 2215 54-55 -0.485129417102776 2215 56-57 -0.43198481888411067 2215 58-59 -0.40161925274831844 2215 60-61 -0.4039567125132777 2215 62-63 -0.43859818917466953 2215 64-65 -0.4914444031679821 2215 66-67 -0.4267705222949303 2215 68-69 -0.41109375892619937 2215 70-71 -0.3871689777082423 2215 72-73 -0.3348986063790065 2215 74-75 -0.3571081156184732 2215 76-77 -0.3535496466905883 2215 78-79 -0.337446017696557 2215 80-81 -0.42661821035419223 2215 82-83 -0.462485162302265 2215 84-85 -0.3598335943902953 2215 86-87 -0.3882264540619289 2215 88-89 -0.33731591722845167 2215 90-91 -0.382133233447302 2215 92-93 -0.36983475058869786 2215 94-95 -0.3715491325457201 2215 96-97 -0.32403652440574504 2215 98-99 -0.36918512104803014 2215 100 -0.36894634962362005 2216 1 -0.35351530653066376 2216 2 -0.33962914747184314 2216 3 -0.2507709822752844 2216 4 -0.2668325833362104 2216 5 -0.2858952688262022 2216 6 -0.25989270609623105 2216 7 -0.3218018515551364 2216 8 -0.2541637199592799 2216 9 -0.40275494252084343 2216 10-11 -0.4285683256074577 2216 12-13 -0.3733278274054541 2216 14-15 -0.5997016145440952 2216 16-17 -0.5781829579347644 2216 18-19 -0.5571278284426455 2216 20-21 -0.5119084222821186 2216 22-23 -0.4593670897455695 2216 24-25 -0.4961800933186282 2216 26-27 -0.4991131472866215 2216 28-29 -0.5414919237262339 2216 30-31 -0.5627379613156265 2216 32-33 -0.5722576943122704 2216 34-35 -0.6044532103281881 2216 36-37 -0.6402589712731626 2216 38-39 -0.607856163372098 2216 40-41 -0.6261777386169101 2216 42-43 -0.6646532690655889 2216 44-45 -0.6607163776593339 2216 46-47 -0.6577804701915326 2216 48-49 -0.6623138137994715 2216 50-51 -0.6534800008939925 2216 52-53 -0.6907900308808195 2216 54-55 -0.6709232728000103 2216 56-57 -0.5937098333421176 2216 58-59 -0.5945992028952745 2216 60-61 -0.6780546878492189 2216 62-63 -0.7002620212393893 2216 64-65 -0.7098422841843686 2216 66-67 -0.6526028052888933 2216 68-69 -0.7078653944184623 2216 70-71 -0.6841473333264076 2216 72-73 -0.669509356988705 2216 74-75 -0.6703294689785793 2216 76-77 -0.5636280086397178 2216 78-79 -0.5791322353676165 2216 80-81 -0.5936615526291433 2216 82-83 -0.6405368787296339 2216 84-85 -0.718665399449165 2216 86-87 -0.6608230475393029 2216 88-89 -0.6818656776176937 2216 90-91 -0.5987787621560088 2216 92-93 -0.6262140352171102 2216 94-95 -0.6233795787719814 2216 96-97 -0.7052684673106917 2216 98-99 -0.7321204798282288 2216 100 -0.7003368461776738 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 435.0 3 48.0 4 76.0 5 145.0 6 484.0 7 1255.0 8 2496.0 9 4182.0 10 5329.0 11 6815.0 12 9766.0 13 14278.0 14 16755.0 15 21541.0 16 27677.0 17 40628.0 18 41028.0 19 21216.0 20 14931.0 21 14403.0 22 15300.0 23 15996.0 24 19005.0 25 22880.0 26 29280.0 27 38109.0 28 50595.0 29 66139.0 30 86753.0 31 112303.0 32 146857.0 33 193792.0 34 266896.0 35 397016.0 36 655998.0 37 1248530.0 38 1858735.0 39 784024.0 40 2370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.633602018121536 20.714497372250925 15.077943989059008 22.57395662056853 2 15.324158715306172 21.00003421773931 40.20184628687961 23.473960780074915 3 17.664907917505186 26.256358663308 31.267994293632334 24.81073912555448 4 12.523084981834218 18.14302887113759 38.56599850401323 30.767887643014962 5 12.481232128644812 37.89438116037537 35.6456747422551 13.978711968724719 6 29.37261834559844 38.77633970658402 17.86459859009897 13.986443357718576 7 25.634938934127018 33.55346745621169 22.921424238247564 17.890169371413734 8 24.551163994751573 36.129999267676425 20.6804661159636 18.63837062160841 9 24.70204183966079 16.947950341425884 22.09528968834035 36.254718130572975 10-11 23.268478458653938 27.431666055331043 29.06559508646055 20.234260399554465 12-13 24.251215129485363 25.107034367721738 29.37905516187389 21.26269534091901 14-15 21.49240190301797 26.947988716460618 27.410911557377233 24.148697823144175 16-17 20.274530201632025 30.235370077642287 28.028365866302018 21.461733854423667 18-19 20.16235517821526 28.395311146380607 31.199998528957 20.242335146447125 20-21 21.16182496315197 27.57155105174777 31.13960581803902 20.127018167061237 22-23 20.92554667635423 27.402181236974478 31.19405839337161 20.478213693299686 24-25 20.734510956552104 27.51243750865437 31.154092393652384 20.59895914114114 26-27 20.542268022115532 27.58576580419842 31.14867991479463 20.723286258891417 28-29 20.567539581449893 27.52963432109607 31.31923295980567 20.58359313764837 30-31 20.7953673658065 27.782645721999096 30.969204674207145 20.452782237987254 32-33 20.546241437170636 27.626699174584985 31.26103881858618 20.566020569658203 34-35 20.752155477732405 27.442914737388445 31.13085950803845 20.6740702768407 36-37 20.954599775570003 27.661876289760933 30.7141705891815 20.669353345487558 38-39 20.830488517390126 27.7954453950438 30.797876133702456 20.576189953863615 40-41 20.804057712214743 27.57335787629999 30.802089392724668 20.820495018760596 42-43 20.781120634160242 27.691808816856106 30.61042208380916 20.916648465174497 44-45 20.971244946887353 27.83222946479938 30.35791435523706 20.83861123307621 46-47 21.128246807756746 27.94333318516306 29.933550429432625 20.994869577647567 48-49 21.207787062048915 27.98838387698499 29.61319883736436 21.190630223601733 50-51 21.222745330797597 28.087023705857916 29.395660359196725 21.294570604147765 52-53 21.22344087830221 28.225781435629237 29.296165086841103 21.254612599227446 54-55 21.304564102777295 28.434533629801795 29.163275539465044 21.097626727955863 56-57 21.235177243092735 28.17109701112844 29.234805005255783 21.358920740523047 58-59 21.14055879806833 27.92399376661519 29.510009967915273 21.425437467401206 60-61 21.250950981329588 28.173223627636805 29.285132264354104 21.290693126679507 62-63 21.12352987640361 28.161639164025452 29.39771502251495 21.317115937055988 64-65 21.113584346567496 28.002846468201646 29.46611852193437 21.417450663296485 66-67 21.094148990432785 28.040262127070616 29.404686487158916 21.46090239533769 68-69 21.37419080642897 28.1388060183567 29.234932922038237 21.252070253176093 70-71 21.375853724600923 27.97347357702973 29.246301526079193 21.404371172290155 72-73 21.239694304473282 28.121625195512806 29.295165736978152 21.34351476303576 74-75 21.292213840740875 28.19409230683625 29.11112779359282 21.402566058830054 76-77 21.402916118889696 28.121833060284303 28.9785956847913 21.4966551360347 78-79 21.43320041713663 28.15863311963769 29.086221667631907 21.32194479559378 80-81 21.316617778385382 28.091854005121476 29.138470960041126 21.453057256452013 82-83 21.263125444063917 28.188399905245042 29.209494075536774 21.338980575154263 84-85 21.271210797824605 28.099040020146987 29.086841900132516 21.542907281895896 86-87 21.35280321379666 28.206689248350987 28.989239908436726 21.45126762941563 88-89 21.214956715534065 28.089847935736195 29.070415116900154 21.624780231829586 90-91 21.156970296244467 28.082943077928707 29.078214282516345 21.68187234331048 92-93 21.189273484788707 28.224134955033563 29.009897340980533 21.576694219197197 94-95 21.07763475131349 28.04468977175345 29.10711357610994 21.770561900823125 96-97 21.085798382292133 28.14963909694127 29.04452550146034 21.72003701930626 98-99 21.035346232961615 28.216121366717918 28.98464495828233 21.763887442038136 100 21.10255453339256 28.05314565359004 28.999475220728687 21.84482459228872 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1990.0 1 1403.5 2 1658.5 3 2416.5 4 1518.5 5 907.5 6 2150.5 7 5556.5 8 9321.5 9 10630.5 10 8002.0 11 4767.5 12 4169.5 13 4582.0 14 5280.5 15 5882.0 16 6602.5 17 7615.0 18 8610.5 19 9691.0 20 11367.5 21 13898.0 22 17677.0 23 23780.0 24 31913.0 25 41837.0 26 54673.0 27 70216.5 28 88769.5 29 108529.0 30 125908.0 31 142460.0 32 158227.0 33 172411.5 34 185017.5 35 195005.5 36 203670.0 37 212262.5 38 219599.5 39 223074.0 40 223988.0 41 223401.5 42 220348.5 43 216217.0 44 211903.0 45 208280.0 46 202670.5 47 194891.0 48 188640.0 49 184287.5 50 178828.5 51 169418.0 52 156234.0 53 145666.0 54 138798.0 55 131456.5 56 121652.0 57 109740.0 58 99800.0 59 91504.5 60 81214.5 61 68966.0 62 58344.5 63 48716.5 64 39633.0 65 32089.0 66 25892.5 67 20432.0 68 15855.0 69 12147.0 70 9105.0 71 6695.5 72 4873.5 73 3489.5 74 2464.0 75 1691.0 76 1031.0 77 693.5 78 446.5 79 216.0 80 117.5 81 71.0 82 38.0 83 23.0 84 13.5 85 6.0 86 5.0 87 3.5 88 3.0 89 2.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11936234763112509 2 0.0 3 0.0 4 0.0 5 0.011080791280424607 6 1.5989597807250516E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.994798903625258E-6 76-77 0.0 78-79 0.0 80-81 1.7588557587975567E-4 82-83 6.315891133863953E-4 84-85 4.557035375066397E-4 86-87 4.7968793421751543E-4 88-89 5.196619287356418E-4 90-91 6.715631079045217E-4 92-93 1.9986997259063144E-4 94-95 2.79817961626884E-4 96-97 5.116671298320165E-4 98-99 9.833602651459067E-4 100 1.2791678245800412E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6254066.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.88709860189163 #Duplication Level Percentage of deduplicated Percentage of total 1 74.1386599026583 43.65810575899948 2 15.60659325279207 18.380539914335664 3 4.765237038541284 8.418329500499 4 1.9878605168187389 4.682373530428493 5 1.0127347115554801 2.981850440846292 6 0.5930478510485931 2.0953720368203066 7 0.39786778647520427 1.64004957008772 8 0.2603545957724473 1.2265221402166193 9 0.18968997139555108 1.0053262844423836 >10 0.9738701682870394 10.005623733192007 >50 0.05245817156478655 2.113949280814597 >100 0.020187753234057644 2.04168197079119 >500 9.498231589420736E-4 0.36048903422377865 >1k 3.799107649084837E-4 0.41520541977834896 >5k 2.7136483155791636E-5 0.09231581062497321 >10k+ 8.14094494673749E-5 0.8822655738991511 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22232 0.3554807384507935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20853 0.333431083074595 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12126 0.19388986301071975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.5989597807250515E-5 0.0 1.5989597807250515E-5 0.0 6 0.0 1.5989597807250515E-5 0.0 1.5989597807250515E-5 0.0 7 0.0 1.5989597807250515E-5 0.0 3.197919561450103E-5 0.0 8 3.197919561450103E-5 1.5989597807250515E-5 0.0 3.197919561450103E-5 0.0 9 3.197919561450103E-5 1.5989597807250515E-5 0.0 4.796879342175154E-5 0.0 10-11 3.197919561450103E-5 1.5989597807250515E-5 0.0 6.395839122900206E-5 0.0 12-13 3.197919561450103E-5 3.997399451812629E-5 0.0 6.395839122900206E-5 0.0 14-15 4.796879342175154E-5 1.119271846507536E-4 0.0 1.0393238574712835E-4 0.0 16-17 4.796879342175154E-5 1.4390638026525463E-4 0.0 1.4390638026525463E-4 0.0 18-19 4.796879342175154E-5 1.7588557587975567E-4 0.0 1.4390638026525463E-4 0.0 20-21 4.796879342175154E-5 1.9187517368700617E-4 0.0 1.5989597807250516E-4 0.0 22-23 4.796879342175154E-5 1.9986997259063144E-4 0.0 2.47838766012383E-4 0.0 24-25 4.796879342175154E-5 2.1585957039788197E-4 0.0 3.197919561450103E-4 0.0 26-27 4.796879342175154E-5 2.238543693015072E-4 0.0 4.3971393969938915E-4 0.0 28-29 4.796879342175154E-5 2.3184916820513245E-4 0.0 0.0012232042322546644 0.0 30-31 4.796879342175154E-5 2.3984396710875771E-4 0.0 0.0028141692140760907 0.0 32-33 4.796879342175154E-5 2.5583356491600825E-4 0.0 0.006643677888912589 0.0 34-35 4.796879342175154E-5 2.5583356491600825E-4 0.0 0.012791678245800412 0.0 36-37 4.796879342175154E-5 2.7981796162688405E-4 0.0 0.021857780202511452 0.0 38-39 4.796879342175154E-5 3.038023583377598E-4 0.0 0.03257880553227292 0.0 40-41 4.796879342175154E-5 3.2778675504863553E-4 0.0 0.0433318100576489 0.0 42-43 4.796879342175154E-5 3.6776074956676187E-4 0.0 0.05482033608215839 0.0 44-45 4.796879342175154E-5 3.6776074956676187E-4 0.0 0.07010639158588988 0.0 46-47 4.796879342175154E-5 3.6776074956676187E-4 0.0 0.0881826319069866 0.0 48-49 4.796879342175154E-5 3.7575554847038713E-4 0.0 0.10683449774914432 0.0 50-51 4.796879342175154E-5 4.157295429885134E-4 0.0 0.1249347224669519 0.0 52-53 4.796879342175154E-5 4.157295429885134E-4 0.0 0.14301895758695224 0.0 54-55 4.796879342175154E-5 4.237243418921386E-4 0.0 0.16487673778946368 0.0 56-57 4.796879342175154E-5 4.3971393969938915E-4 0.0 0.18996441674903974 0.0 58-59 5.59635923253768E-5 4.6369833641026495E-4 0.0 0.21709876422794386 0.0 60-61 6.395839122900206E-5 4.6369833641026495E-4 0.0 0.24583207148757305 0.0 62-63 6.395839122900206E-5 4.7968793421751543E-4 0.0 0.27461334754062394 0.0 64-65 6.395839122900206E-5 4.7968793421751543E-4 0.0 0.3086072324788386 0.0 66-67 6.395839122900206E-5 5.59635923253768E-4 0.0 0.34838935182327785 0.0 68-69 6.395839122900206E-5 5.59635923253768E-4 0.0 0.39368788241121855 0.0 70-71 6.395839122900206E-5 5.59635923253768E-4 0.0 0.44278394247838126 0.0 72-73 6.395839122900206E-5 5.836203199646437E-4 0.0 0.4946701873629092 0.0 74-75 6.395839122900206E-5 5.996099177718944E-4 0.0 0.5492027106845371 0.0 76-77 6.395839122900206E-5 6.076047166755196E-4 0.0 0.6098032863740165 0.0 78-79 6.395839122900206E-5 6.235943144827701E-4 0.0 0.6801495219270152 0.0 80-81 6.395839122900206E-5 6.315891133863954E-4 0.0 0.7591301402959291 0.0 82-83 6.395839122900206E-5 6.555735100972711E-4 0.0 0.840988886270148 0.0 84-85 6.395839122900206E-5 6.715631079045216E-4 0.0 0.9266531565224927 0.0 86-87 6.395839122900206E-5 7.035423035190227E-4 0.0 1.0204241528631135 0.0 88 6.395839122900206E-5 7.195319013262732E-4 0.0 1.0987571925208337 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12095 0.0 58.24664 1 GTATCAA 22770 0.0 41.41805 1 ATCAACG 27565 0.0 33.71076 3 TCAACGC 27680 0.0 33.67259 4 TATCAAC 28610 0.0 32.594456 2 CAACGCA 28775 0.0 32.37488 5 GTGGTAT 5020 0.0 32.34226 1 AACGCAG 30050 0.0 31.077208 6 TGGTATC 5205 0.0 29.799782 2 ACGCAGA 34145 0.0 27.171188 7 CGCAGAG 34370 0.0 26.91127 8 GCAGAGT 37055 0.0 24.66956 9 GTACATG 19315 0.0 22.537304 1 GAGTACT 26470 0.0 21.812721 12-13 CAGAGTA 36205 0.0 21.691847 10-11 TACATGG 19435 0.0 21.5483 2 AGAGTAC 34730 0.0 20.725302 10-11 ACATGGG 19520 0.0 20.515383 3 GTACTTT 28990 0.0 19.762602 14-15 AGTACTT 27360 0.0 19.30807 12-13 >>END_MODULE