FastQCFastQC Report
Wed 25 May 2016
SRR2049472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6488783
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65850.042.6346131
GTATCAA111000.033.8506931
TCAACGC115750.031.0629754
ATCAACG116350.030.9840583
CAACGCA126900.028.2976975
TATCAAC134500.027.0824992
AACGCAG134250.026.9591276
ACGCAGA151650.023.772917
GTACATG189350.023.5940251
CGCAGAG156150.022.9647528
GTGGTAT39250.022.7644771
TACATGG188600.022.553622
ACATGGG196200.021.1050423
GCAGAGT180800.018.9760999
TGGTATC43650.018.4128592
CATGGGG132800.017.5544094
TAGACCG2705.451693E-717.408275
GTACCTG45450.017.2793061
AGAGTAC161150.016.43423510-11
CAGAGTA175550.016.30430810-11
Produced by FastQC (version 0.11.5)