##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049472_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6488783 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.82261373203573 31.0 31.0 34.0 28.0 34.0 2 31.004370619267124 31.0 31.0 34.0 28.0 34.0 3 31.08056148587493 31.0 31.0 34.0 28.0 34.0 4 34.77805668643874 37.0 35.0 37.0 32.0 37.0 5 34.80553764858526 37.0 35.0 37.0 32.0 37.0 6 34.926781801764676 37.0 35.0 37.0 32.0 37.0 7 34.86435915640884 37.0 35.0 37.0 32.0 37.0 8 34.80980701620011 37.0 35.0 37.0 32.0 37.0 9 36.43686805368588 39.0 37.0 39.0 32.0 39.0 10-11 36.37771058147575 39.0 37.0 39.0 32.0 39.0 12-13 36.30523258367555 39.0 37.0 39.0 32.0 39.0 14-15 37.550830872291456 40.0 38.0 41.0 32.0 41.0 16-17 37.534931511810456 40.0 38.0 41.0 32.0 41.0 18-19 37.541117186381484 40.0 38.0 41.0 32.0 41.0 20-21 37.59037195418617 40.0 38.0 41.0 32.0 41.0 22-23 37.55248619039965 40.0 38.0 41.0 32.0 41.0 24-25 37.554374217784755 40.0 38.0 41.0 32.0 41.0 26-27 37.55239765299595 40.0 38.0 41.0 32.0 41.0 28-29 37.53655554516155 40.0 38.0 41.0 32.0 41.0 30-31 37.483967101380955 40.0 38.0 41.0 32.0 41.0 32-33 37.44723756365408 40.0 38.0 41.0 32.0 41.0 34-35 37.386782775753176 40.0 38.0 41.0 32.0 41.0 36-37 37.2839883842625 40.0 38.0 41.0 31.0 41.0 38-39 37.184250883409106 40.0 37.0 41.0 31.0 41.0 40-41 37.07393921171351 40.0 37.0 41.0 31.0 41.0 42-43 36.98324300566069 40.0 37.0 41.0 30.5 41.0 44-45 36.83251296891883 40.0 37.0 41.0 30.0 41.0 46-47 36.71403474272448 40.0 37.0 41.0 30.0 41.0 48-49 36.617760834350605 40.0 36.0 41.0 30.0 41.0 50-51 36.506480491025826 40.0 36.0 41.0 30.0 41.0 52-53 36.40010276195089 40.0 36.0 41.0 30.0 41.0 54-55 36.256915588023205 39.5 35.5 41.0 30.0 41.0 56-57 36.09634996269716 39.0 35.0 41.0 29.0 41.0 58-59 35.91756296982038 39.0 35.0 41.0 29.0 41.0 60-61 35.724508432474934 39.0 35.0 41.0 29.0 41.0 62-63 35.47289692073228 38.5 35.0 40.0 28.0 41.0 64-65 35.23373050077341 38.0 35.0 40.0 28.0 41.0 66-67 35.00920812115307 37.5 34.0 40.0 28.0 41.0 68-69 34.72491875595162 37.0 34.0 40.0 28.0 41.0 70-71 34.412238319573945 36.5 34.0 39.5 28.0 41.0 72-73 34.07774501011977 36.0 34.0 39.0 27.0 41.0 74-75 33.77841083605354 36.0 34.0 39.0 27.0 40.5 76-77 33.45225861305579 35.0 34.0 38.5 26.0 40.0 78-79 33.10568792329779 35.0 33.0 37.0 26.0 39.0 80-81 32.77449569510954 35.0 33.0 37.0 26.0 39.0 82-83 32.47742311616832 35.0 33.0 36.5 26.0 39.0 84-85 32.191048537144795 35.0 33.0 36.0 26.0 37.5 86-87 31.920684048148935 35.0 33.0 36.0 25.0 37.0 88-89 31.698567820807074 35.0 33.0 35.0 25.0 37.0 90-91 31.493330952815036 35.0 33.0 35.0 25.0 36.0 92-93 31.295271470782733 35.0 33.0 35.0 24.0 36.0 94-95 31.098046659905254 35.0 32.0 35.0 23.5 36.0 96-97 30.91590657292747 35.0 32.0 35.0 21.5 36.0 98-99 30.748348650278487 35.0 32.0 35.0 19.5 35.0 100 30.62069959806022 35.0 32.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2737629972712199 1101 2 0.2627803599886178 1101 3 0.24190519561227575 1101 4 0.3516376492269515 1101 5 0.3984385714429024 1101 6 0.4948612715331109 1101 7 0.5203870556091275 1101 8 0.5037691684035721 1101 9 0.6070318632275971 1101 10-11 0.6411002721641665 1101 12-13 0.5996056684643065 1101 14-15 0.7358644250286446 1101 16-17 0.6869481012686265 1101 18-19 0.6967375546201211 1101 20-21 0.731613887122009 1101 22-23 0.6895548842413177 1101 24-25 0.6846297726814967 1101 26-27 0.6950858056729814 1101 28-29 0.678203784238562 1101 30-31 0.6741025773854687 1101 32-33 0.6795172215782443 1101 34-35 0.6767008976247482 1101 36-37 0.6394665995212421 1101 38-39 0.6971974382492974 1101 40-41 0.7145397861672791 1101 42-43 0.6478628864532254 1101 44-45 0.6727490110811587 1101 46-47 0.6601609139736766 1101 48-49 0.5907496436864434 1101 50-51 0.6570641065605827 1101 52-53 0.6219017015514083 1101 54-55 0.569690199767777 1101 56-57 0.5930537149061266 1101 58-59 0.6172835688291585 1101 60-61 0.6150643292527747 1101 62-63 0.598578926493758 1101 64-65 0.5954033100683276 1101 66-67 0.5951587904160363 1101 68-69 0.5809786919534616 1101 70-71 0.6248775859345557 1101 72-73 0.5388305224152319 1101 74-75 0.5385892189742592 1101 76-77 0.5000739792608471 1101 78-79 0.47959514263138203 1101 80-81 0.5067569274176051 1101 82-83 0.47335112559032666 1101 84-85 0.508965042719737 1101 86-87 0.4675404126752625 1101 88-89 0.3826975494003584 1101 90-91 0.4900089074081819 1101 92-93 0.45544114601627683 1101 94-95 0.4780396846666797 1101 96-97 0.5305903567019534 1101 98-99 0.5784368654355099 1101 100 0.5185249323521859 1102 1 0.02735223703601264 1102 2 0.1428204962229529 1102 3 0.13268188782904033 1102 4 0.22228630522882042 1102 5 0.1860171675558533 1102 6 0.24148132356715024 1102 7 0.28106805064434326 1102 8 0.3101262188219067 1102 9 0.4264468756551949 1102 10-11 0.4042642190459702 1102 12-13 0.39829542404017104 1102 14-15 0.4966228616343571 1102 16-17 0.4528919623485237 1102 18-19 0.49321478585854095 1102 20-21 0.49275611333631275 1102 22-23 0.5162765313760147 1102 24-25 0.5197942311540302 1102 26-27 0.4310013920179401 1102 28-29 0.4711415172762088 1102 30-31 0.42531826198272427 1102 32-33 0.4224349099307503 1102 34-35 0.39779595052847583 1102 36-37 0.43882857110354934 1102 38-39 0.48478597366273135 1102 40-41 0.4534607942461477 1102 42-43 0.4124849428785353 1102 44-45 0.3634198265732067 1102 46-47 0.33839998844867836 1102 48-49 0.3214453656235392 1102 50-51 0.33297627105672234 1102 52-53 0.33055265368619047 1102 54-55 0.3076064437030368 1102 56-57 0.33461147005692027 1102 58-59 0.33595022660367135 1102 60-61 0.3148286905828144 1102 62-63 0.3003692764681105 1102 64-65 0.31772708324514554 1102 66-67 0.3428313783222592 1102 68-69 0.2696544696799066 1102 70-71 0.2605761195864673 1102 72-73 0.2822462508329622 1102 74-75 0.2532219884720206 1102 76-77 0.2191310700106044 1102 78-79 0.2089127329195577 1102 80-81 0.2407918487636138 1102 82-83 0.15158761867964188 1102 84-85 0.1713521045211479 1102 86-87 0.1513762274304966 1102 88-89 0.15388066424889502 1102 90-91 0.14942793572517132 1102 92-93 0.08912513023173574 1102 94-95 0.13598262386357618 1102 96-97 0.1442520900165647 1102 98-99 0.16948089007354028 1102 100 0.11035522389478558 1103 1 0.2049790451910276 1103 2 0.21732144910262363 1103 3 0.18241868156226815 1103 4 0.34975452538861873 1103 5 0.3373199018311581 1103 6 0.3778860887970339 1103 7 0.41604241216838034 1103 8 0.3852670910998057 1103 9 0.5295057761263422 1103 10-11 0.5345309004388241 1103 12-13 0.4679548613136504 1103 14-15 0.6023787779759644 1103 16-17 0.5599620721337715 1103 18-19 0.5033089731918565 1103 20-21 0.553140500138241 1103 22-23 0.5905023011224344 1103 24-25 0.6117489386692156 1103 26-27 0.5872923518726978 1103 28-29 0.5273294663183634 1103 30-31 0.565882330323177 1103 32-33 0.5819399907427538 1103 34-35 0.5751216103313723 1103 36-37 0.5646254540491356 1103 38-39 0.5820902575976632 1103 40-41 0.6051765194437166 1103 42-43 0.584318282424654 1103 44-45 0.5321433075933868 1103 46-47 0.4832782801455906 1103 48-49 0.49101893419463494 1103 50-51 0.4881131408730397 1103 52-53 0.4637865733236026 1103 54-55 0.3974378042089626 1103 56-57 0.38014362193552387 1103 58-59 0.41355701009524637 1103 60-61 0.45580243237644424 1103 62-63 0.486195064268486 1103 64-65 0.4894756945074903 1103 66-67 0.453677484539611 1103 68-69 0.44441686877618025 1103 70-71 0.4295054628450856 1103 72-73 0.44837322850014516 1103 74-75 0.47130179214902057 1103 76-77 0.40619799897931586 1103 78-79 0.3823786008516947 1103 80-81 0.42538258668896134 1103 82-83 0.4555232193521448 1103 84-85 0.4546684919917965 1103 86-87 0.45380679678739 1103 88-89 0.43687864264378007 1103 90-91 0.4285607043342452 1103 92-93 0.39820959117816557 1103 94-95 0.48355035623433906 1103 96-97 0.49914265659617385 1103 98-99 0.4472405600850742 1103 100 0.3744429489960268 1104 1 0.10244499938082186 1104 2 0.13082979715926157 1104 3 0.14393943371218754 1104 4 0.2641068458440188 1104 5 0.2803591849583853 1104 6 0.3314383162876098 1104 7 0.34469173390969843 1104 8 0.2943310514855355 1104 9 0.355066963109941 1104 10-11 0.4339991920426911 1104 12-13 0.4710817278722317 1104 14-15 0.5271001399932516 1104 16-17 0.478350601126607 1104 18-19 0.48100676023735645 1104 20-21 0.4664696219721307 1104 22-23 0.4714461795375158 1104 24-25 0.4799835398327801 1104 26-27 0.5053381539538435 1104 28-29 0.43937197503354497 1104 30-31 0.42837389452466823 1104 32-33 0.4458180936771683 1104 34-35 0.422489472962269 1104 36-37 0.4710382275649607 1104 38-39 0.4067318432263747 1104 40-41 0.47878273628086276 1104 42-43 0.4693864181993348 1104 44-45 0.40275420574013765 1104 46-47 0.3913457942068064 1104 48-49 0.33939332543672407 1104 50-51 0.3360446139552238 1104 52-53 0.34722467251646094 1104 54-55 0.31570046399859564 1104 56-57 0.3448951271565832 1104 58-59 0.35101329190115393 1104 60-61 0.42025225409292943 1104 62-63 0.40932418057009556 1104 64-65 0.4305840426444121 1104 66-67 0.41271545588341496 1104 68-69 0.37743377453963234 1104 70-71 0.3900776654352569 1104 72-73 0.3563455393296806 1104 74-75 0.3807201791491366 1104 76-77 0.30808675364890803 1104 78-79 0.3169631900387131 1104 80-81 0.35570823026380793 1104 82-83 0.3633171126669339 1104 84-85 0.2727503754366154 1104 86-87 0.30368669538414395 1104 88-89 0.2955377632107776 1104 90-91 0.34477994198185513 1104 92-93 0.34240727473856225 1104 94-95 0.3764434680455899 1104 96-97 0.38368655478387126 1104 98-99 0.36140000663223404 1104 100 0.3132625826366606 1105 1 0.016090743562820364 1105 2 0.041116583928154427 1105 3 0.06838131464153818 1105 4 0.1385051547299696 1105 5 0.10542947108800149 1105 6 0.19262493582497342 1105 7 0.2393501425755744 1105 8 0.18566580301831692 1105 9 0.25530260014803474 1105 10-11 0.2891166004672314 1105 12-13 0.27768034433985633 1105 14-15 0.3063340805160166 1105 16-17 0.2936382446118131 1105 18-19 0.25835878795668066 1105 20-21 0.2821951957093347 1105 22-23 0.2633189155179281 1105 24-25 0.3004126798946771 1105 26-27 0.3009430055052533 1105 28-29 0.31260648810935265 1105 30-31 0.3162528883914817 1105 32-33 0.3300104124203074 1105 34-35 0.35584301581005917 1105 36-37 0.31450210122772404 1105 38-39 0.30224688300327074 1105 40-41 0.33714959399206634 1105 42-43 0.29576856362447046 1105 44-45 0.2633949945558527 1105 46-47 0.168564994255199 1105 48-49 0.19802082805416887 1105 50-51 0.22849015975123166 1105 52-53 0.22834962188521502 1105 54-55 0.22616246831441345 1105 56-57 0.23671334028095004 1105 58-59 0.19173919540682505 1105 60-61 0.1749532328286989 1105 62-63 0.23215214337730572 1105 64-65 0.22292929338030376 1105 66-67 0.17582701889062236 1105 68-69 0.21021086620079643 1105 70-71 0.2625161952580015 1105 72-73 0.2727537888971767 1105 74-75 0.2492931635687654 1105 76-77 0.20977689401711075 1105 78-79 0.24487920023132403 1105 80-81 0.2488351441777894 1105 82-83 0.26290956201543736 1105 84-85 0.16924276828308393 1105 86-87 0.19923822272114222 1105 88-89 0.21181619591333956 1105 90-91 0.23181727923685713 1105 92-93 0.2102713563673504 1105 94-95 0.24715722073359814 1105 96-97 0.20489830557562527 1105 98-99 0.2337680211064601 1105 100 0.14148640968033277 1106 1 0.08903613652080722 1106 2 0.11573505072663082 1106 3 0.12597421902958317 1106 4 0.21661318845911381 1106 5 0.20218604414949937 1106 6 0.1825058877004011 1106 7 0.21840243924741287 1106 8 0.13705471802615676 1106 9 0.2409040045559152 1106 10-11 0.23973382770338247 1106 12-13 0.22337884720855072 1106 14-15 0.28806485272692584 1106 16-17 0.2759821636196378 1106 18-19 0.2859037216223257 1106 20-21 0.29039347040101404 1106 22-23 0.3378432658465371 1106 24-25 0.3407870902783756 1106 26-27 0.33698505268065304 1106 28-29 0.25637420808818234 1106 30-31 0.2600543372071584 1106 32-33 0.26481666280835015 1106 34-35 0.2659600388808556 1106 36-37 0.2515873036777876 1106 38-39 0.24551213927144744 1106 40-41 0.3630608243480111 1106 42-43 0.3187705604752935 1106 44-45 0.27870593422834133 1106 46-47 0.26808537416587086 1106 48-49 0.21318859113046784 1106 50-51 0.2583700598340215 1106 52-53 0.2703348710008555 1106 54-55 0.20736067675065328 1106 56-57 0.19984381157284048 1106 58-59 0.2341074565316461 1106 60-61 0.2369053632707505 1106 62-63 0.2256452628655552 1106 64-65 0.220360844660064 1106 66-67 0.2632205804483263 1106 68-69 0.22457777345142915 1106 70-71 0.1943002789949091 1106 72-73 0.2237614070149192 1106 74-75 0.25337170018372746 1106 76-77 0.205450839799866 1106 78-79 0.18133819671415807 1106 80-81 0.26615594972256673 1106 82-83 0.24836271064812365 1106 84-85 0.178682711976883 1106 86-87 0.22896371796839787 1106 88-89 0.24568458610224297 1106 90-91 0.24839900081691013 1106 92-93 0.16359873093950483 1106 94-95 0.20098725203623147 1106 96-97 0.2669318297232124 1106 98-99 0.2568734655572875 1106 100 0.24936603179250127 1107 1 0.08715973199796068 1107 2 0.11931758107007795 1107 3 0.21735769156252616 1107 4 0.18827700236795408 1107 5 0.15978767791465032 1107 6 0.23030748133126622 1107 7 0.22272582848775357 1107 8 0.2141699926172862 1107 9 0.2473409603398764 1107 10-11 0.25729632431358596 1107 12-13 0.28451885866481064 1107 14-15 0.3147847760500895 1107 16-17 0.3113234808959362 1107 18-19 0.33050622064076407 1107 20-21 0.2858078620507456 1107 22-23 0.3119274131344696 1107 24-25 0.30988475873233057 1107 26-27 0.3622297747709027 1107 28-29 0.29034296801735593 1107 30-31 0.3254424431759304 1107 32-33 0.3052873294166645 1107 34-35 0.29211950039263 1107 36-37 0.2805691607134193 1107 38-39 0.3022154194913398 1107 40-41 0.3484995797763446 1107 42-43 0.3117071559911295 1107 44-45 0.3241928604612525 1107 46-47 0.2568348484930496 1107 48-49 0.2707549828619662 1107 50-51 0.28146366732183026 1107 52-53 0.23413330864089232 1107 54-55 0.23007594259006225 1107 56-57 0.23346433271477451 1107 58-59 0.2398064520536991 1107 60-61 0.2543909440646601 1107 62-63 0.2443989535257174 1107 64-65 0.24245929707669234 1107 66-67 0.25419386606998984 1107 68-69 0.21699864275592518 1107 70-71 0.22554001702185644 1107 72-73 0.20832501086074728 1107 74-75 0.2440200541906279 1107 76-77 0.22683659302177261 1107 78-79 0.22399104699776728 1107 80-81 0.1587435678360336 1107 82-83 0.12852867403437074 1107 84-85 0.16218160961525996 1107 86-87 0.14120103239294934 1107 88-89 0.10481096306658699 1107 90-91 0.16035819658183215 1107 92-93 0.1166773883415928 1107 94-95 0.13220444190364944 1107 96-97 0.15977589651583912 1107 98-99 0.2170474500033457 1107 100 0.1619971150450361 1108 1 0.11017741753420296 1108 2 0.0732721182706193 1108 3 0.14094161224277002 1108 4 0.1398006830200913 1108 5 0.11673146655436284 1108 6 0.16065810972136774 1108 7 0.09135267187662777 1108 8 0.1291018273596194 1108 9 0.170225306964511 1108 10-11 0.18350270783810885 1108 12-13 0.16679885828172303 1108 14-15 0.18415377092122043 1108 16-17 0.15065245519129178 1108 18-19 0.22308311592484387 1108 20-21 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-0.7204079509892125 2215 52-53 -0.6501949910608928 2215 54-55 -0.7633126806600359 2215 56-57 -0.6730578315460889 2215 58-59 -0.6940383227311742 2215 60-61 -0.6805535264293638 2215 62-63 -0.7025093608223685 2215 64-65 -0.7085090196948727 2215 66-67 -0.6673974880456441 2215 68-69 -0.630764453178358 2215 70-71 -0.5947315015397834 2215 72-73 -0.535380911308124 2215 74-75 -0.5563701689133538 2215 76-77 -0.5907882800022151 2215 78-79 -0.5267222954123554 2215 80-81 -0.5598678294859312 2215 82-83 -0.6038563124771308 2215 84-85 -0.6204214658071763 2215 86-87 -0.6432050773579832 2215 88-89 -0.6198306817046308 2215 90-91 -0.6350074266047869 2215 92-93 -0.6797879305541699 2215 94-95 -0.7130599642449873 2215 96-97 -0.7551921954683607 2215 98-99 -0.6676729732090614 2215 100 -0.6884275850733026 2216 1 -0.3481612596532564 2216 2 -0.5239177959487868 2216 3 -0.4710389453127277 2216 4 -0.6000976178280766 2216 5 -0.6639675644349552 2216 6 -0.7308580684583035 2216 7 -0.6866865551038472 2216 8 -0.6249303687074246 2216 9 -0.8135178513226933 2216 10-11 -0.8143632699710963 2216 12-13 -0.8376593090020208 2216 14-15 -0.9911068902193492 2216 16-17 -0.9792254574699868 2216 18-19 -1.062873784128719 2216 20-21 -1.0188589296759574 2216 22-23 -1.0691633434224812 2216 24-25 -1.0268605681727507 2216 26-27 -1.04317675975485 2216 28-29 -1.0044874855874966 2216 30-31 -1.2019712909818736 2216 32-33 -1.1556970977693268 2216 34-35 -1.1348410816415893 2216 36-37 -1.1275850444295372 2216 38-39 -1.0712995026261112 2216 40-41 -1.1109813082301727 2216 42-43 -1.055723511230937 2216 44-45 -1.1104423941005237 2216 46-47 -0.9556875809561305 2216 48-49 -1.101697880006121 2216 50-51 -1.0385881943793436 2216 52-53 -1.1452976934851904 2216 54-55 -1.0881343448590073 2216 56-57 -1.0213092513696225 2216 58-59 -1.0226525023579853 2216 60-61 -1.0548435857192615 2216 62-63 -1.1109658831068643 2216 64-65 -1.1215515988183924 2216 66-67 -1.091584411734992 2216 68-69 -0.9640336946608699 2216 70-71 -0.9829515619063116 2216 72-73 -1.0191721781116456 2216 74-75 -1.0950507459609895 2216 76-77 -1.100819304075955 2216 78-79 -1.1390305740382374 2216 80-81 -1.1088508052359458 2216 82-83 -1.0400179708875434 2216 84-85 -0.9471054441838334 2216 86-87 -1.0387244741352504 2216 88-89 -1.043857822582833 2216 90-91 -1.0593650412018292 2216 92-93 -1.010983660467442 2216 94-95 -1.0328862918632673 2216 96-97 -1.1388000702153676 2216 98-99 -1.142989061445963 2216 100 -1.1753957816296356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93456.0 3 17370.0 4 11071.0 5 8977.0 6 7667.0 7 7974.0 8 8307.0 9 9275.0 10 10691.0 11 11744.0 12 13222.0 13 14660.0 14 16784.0 15 17525.0 16 17737.0 17 19286.0 18 18396.0 19 16654.0 20 16865.0 21 18757.0 22 20937.0 23 25430.0 24 31012.0 25 38349.0 26 46386.0 27 54012.0 28 65245.0 29 82049.0 30 100415.0 31 118118.0 32 152358.0 33 203163.0 34 285282.0 35 421669.0 36 713268.0 37 1307641.0 38 1798221.0 39 667520.0 40 1290.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.163125899843685 20.503442058742106 14.401169690758378 22.93226235065583 2 15.430241861587767 20.654466953978034 40.45573906279116 23.459552121643036 3 18.143010509879844 25.803099863511058 30.497533411275633 25.556356215333466 4 12.629144364256465 17.562523812870605 39.235734780416045 30.572597042456884 5 13.046460257463844 37.79375581439077 35.550811225033954 13.608972703111426 6 29.86202194172321 38.255420005123085 18.426075454843335 13.456482598310368 7 26.37306807232435 32.64453147338642 23.16025733807661 17.822143116212615 8 24.40680839651889 36.09519794888077 21.1729048197757 18.325088834824637 9 24.559023191962336 17.40615549602483 22.16697109331423 35.86785021869861 10-11 23.231045721160033 27.331592661124564 29.528269097236944 19.90909252047846 12-13 23.855614895369744 25.000599203933486 29.28795536897899 21.85583053171777 14-15 21.413743203142857 27.306615841379976 26.482315571455413 24.797325384021754 16-17 20.957615992438924 30.43977110854157 27.1275620125013 21.47505088651821 18-19 20.901671653785236 29.44869818211186 28.969174960356973 20.68045520374593 20-21 22.027361911144457 28.77045877849371 28.422296562043574 20.77988274831826 22-23 21.71512080897833 28.599272767851847 28.489082809241285 21.19652361392854 24-25 21.486199136852147 28.722821242972056 28.480457328560554 21.31052229161524 26-27 21.28860636782075 28.651402000954008 28.535610831937895 21.524380799287343 28-29 21.311749812789156 28.655208445800483 28.669311393154643 21.363730348255714 30-31 21.601805276497313 29.014870627605156 28.24731061150181 21.136013484395722 32-33 21.232300514290216 28.795042394609965 28.62249230152231 21.350164789577512 34-35 21.44084262643685 28.44865274049101 28.658695927423206 21.451808705648933 36-37 21.716567386181424 28.77458183247652 28.19394955333222 21.314901228009838 38-39 21.531552910005402 28.912540725185565 28.338686801059172 21.21721956374986 40-41 21.42748369863848 28.611958621784524 28.491658773835514 21.468898905741483 42-43 21.361324343510844 28.643422716779593 28.431677626519175 21.563575313190388 44-45 21.5608035696738 28.75602078019369 28.28326790549875 21.399907744633754 46-47 21.5646687424861 28.824002126531884 28.173772922432995 21.437556208549026 48-49 21.438853622667235 28.675047632905475 28.288188750387853 21.597909994039433 50-51 21.492495537796735 28.603044663338988 28.253605269888833 21.65085452897544 52-53 21.512874469815614 28.62141810056699 28.201809689872476 21.663897739744915 54-55 21.60551716867175 28.93241340447942 28.055736424615695 21.406333002233136 56-57 21.522515073658845 28.711697738843274 28.10559145035758 21.6601957371403 58-59 21.421327972148138 28.404050787417656 28.394179651589745 21.78044158884446 60-61 21.650261193949337 28.740366093828847 28.020691764013954 21.588680948207863 62-63 21.49632913186433 28.766430625127693 28.11855012442256 21.61869011858542 64-65 21.52545564285434 28.49979008778084 28.255325400164676 21.71942886920014 66-67 21.38235153585196 28.601603284868983 28.16016048261926 21.855884696659796 68-69 21.75397453553673 28.711995394478766 28.061866268795665 21.472163801188834 70-71 21.696992772989873 28.491183880063925 28.15009274142734 21.661730605518855 72-73 21.466096057567118 28.611904501512036 28.33266550581056 21.589333935110286 74-75 21.515832195567086 28.664742194306967 28.155662607702435 21.663763002423508 76-77 21.595742459127656 28.666562322729938 27.98857333710328 21.749121881039127 78-79 21.66256448623022 28.665848850796078 28.14129688087863 21.53028978209507 80-81 21.543871331467443 28.57338837745842 28.20612182847921 21.676618462594927 82-83 21.435194101227136 28.632981830206827 28.34786286391933 21.583961204646705 84-85 21.482472308442922 28.57220142281941 28.17451543676524 21.770810831972433 86-87 21.63275919685014 28.699941858816192 28.0687354569971 21.598563487336566 88-89 21.461680379242235 28.60061751482152 28.16948387348861 21.768218232447634 90-91 21.381596338349684 28.56117303517302 28.1954241701906 21.861806456286697 92-93 21.45912229242955 28.733726526580462 28.066319340998785 21.740831839991195 94-95 21.401652165958296 28.522794330547097 28.25460578889271 21.82094771460189 96-97 21.379171677928422 28.59609762007261 28.1849930493549 21.83973765264407 98-99 21.388163950862367 28.639413449463845 28.1371402501733 21.83528234950049 100 21.475171979481754 28.562788809473826 28.10903177023658 21.853007440807833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3283.0 1 2374.0 2 1709.0 3 1920.5 4 1687.5 5 1416.5 6 1319.0 7 1305.0 8 1388.5 9 1408.0 10 1212.0 11 1048.5 12 1076.0 13 1139.0 14 1242.5 15 1356.5 16 1615.5 17 2141.5 18 2805.5 19 3841.0 20 5513.0 21 8009.0 22 11770.0 23 17601.0 24 25336.0 25 35317.0 26 48978.0 27 65857.0 28 85687.0 29 106806.0 30 126691.5 31 145207.5 32 162829.5 33 178408.5 34 192087.5 35 203911.0 36 213655.5 37 222728.0 38 231124.5 39 235148.0 40 236661.0 41 236930.5 42 233758.0 43 229893.5 44 224294.5 45 219553.5 46 215252.5 47 207563.5 48 201660.0 49 197914.5 50 191301.0 51 181754.5 52 169498.0 53 158400.0 54 151277.0 55 144354.0 56 134177.5 57 121364.5 58 109589.0 59 99785.0 60 88755.5 61 75495.0 62 62845.5 63 51745.0 64 42231.0 65 34007.5 66 27172.0 67 21454.0 68 16509.5 69 12639.5 70 9485.0 71 7103.0 72 5305.0 73 3816.0 74 2736.0 75 1899.0 76 1230.0 77 831.0 78 561.5 79 388.5 80 271.0 81 192.0 82 150.0 83 127.0 84 109.5 85 79.0 86 52.0 87 38.5 88 36.0 89 28.5 90 27.5 91 25.0 92 14.5 93 11.0 94 13.5 95 12.5 96 13.0 97 12.5 98 13.5 99 14.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07733345374625719 2 0.012575547679742104 3 0.016613284802404395 4 0.010710791222329364 5 0.008799801133741104 6 0.00778266124787961 7 0.019818816563907285 8 0.009524128022157621 9 0.009709062546859711 10-11 0.013376930620117824 12-13 0.015542205680171459 14-15 0.010387155804100708 16-17 0.012590958890133943 18-19 0.008745861897369662 20-21 0.013238229726591258 22-23 0.013808444511089368 24-25 0.015696317784089867 26-27 0.005825437528115827 28-29 0.011982216079656232 30-31 0.010649146380762002 32-33 0.012513902838174738 34-35 0.00914655336755752 36-37 0.010533562302823195 38-39 0.01916384012225405 40-41 0.012760482204444193 42-43 0.008584044188255332 44-45 0.02088989568614022 46-47 0.018871027124809075 48-49 0.01766124710904957 50-51 0.01042568383008031 52-53 0.010941959378206977 54-55 0.012598664495329863 56-57 0.02014245198213594 58-59 0.002589083345829256 60-61 0.007721016406312247 62-63 0.015603850521738821 64-65 0.023848848081373657 66-67 0.013145762464240212 68-69 0.014147491139709865 70-71 0.025590314855651667 72-73 0.02048920421595236 74-75 0.019888167010670567 76-77 0.009624300889704587 78-79 0.011866632001717425 80-81 0.019425830698915344 82-83 0.012167150604358322 84-85 0.011550702188684689 86-87 0.017368434111604596 88-89 0.015465149628212256 90-91 0.015380387971057131 92-93 0.008322053611594039 94-95 0.015357271155469369 96-97 0.013808444511089368 98-99 0.010487328671647672 100 0.007844306089446973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6488783.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.63166601797741 #Duplication Level Percentage of deduplicated Percentage of total 1 76.47574746230973 48.66279220996876 2 14.775981822100235 18.804406807831747 3 4.209092421272469 8.034946895676288 4 1.6626235707802628 4.231820310780268 5 0.8753510090974864 2.785002152969538 6 0.48626943951985724 1.856528074216597 7 0.3076868303695584 1.3705037939744067 8 0.20731744571197172 1.0553563572195475 9 0.15606833850274365 0.8937799552428504 >10 0.7872905830134133 8.721791254829593 >50 0.04123219791919337 1.7672678664439907 >100 0.014926993117550953 1.5877058204096635 >500 3.392102445120363E-4 0.12958128726509094 >1k 7.267604100842012E-5 0.09851721317159146 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.5411210391840812E-5 0.0 4 0.0 0.0 0.0 6.164484156736325E-5 0.0 5 0.0 0.0 0.0 6.164484156736325E-5 0.0 6 0.0 0.0 0.0 6.164484156736325E-5 0.0 7 0.0 0.0 0.0 7.705605195920406E-5 0.0 8 0.0 0.0 0.0 7.705605195920406E-5 0.0 9 1.5411210391840812E-5 0.0 0.0 7.705605195920406E-5 0.0 10-11 2.311681558776122E-5 0.0 0.0 1.3099528833064692E-4 0.0 12-13 3.0822420783681625E-5 1.5411210391840812E-5 0.0 1.6181770911432854E-4 0.0 14-15 3.0822420783681625E-5 4.623363117552244E-5 0.0 2.2346255068169177E-4 0.0 16-17 3.0822420783681625E-5 6.164484156736325E-5 0.0 2.46579366269453E-4 0.0 18-19 4.623363117552244E-5 8.476165715512446E-5 0.0 2.542849714653734E-4 0.0 20-21 6.164484156736325E-5 9.246726235104487E-5 0.0 3.3134102342457746E-4 0.0 22-23 6.164484156736325E-5 9.246726235104487E-5 0.0 4.2380828577562237E-4 0.0 24-25 6.164484156736325E-5 9.246726235104487E-5 0.0 5.625091793021896E-4 0.0 26-27 6.164484156736325E-5 1.0787847274288569E-4 0.0 7.705605195920406E-4 0.0 28-29 6.935044676328365E-5 1.155840779388061E-4 0.0 0.0015719434599677628 0.0 30-31 7.705605195920406E-5 1.232896831347265E-4 0.0 0.0031824149459151276 0.0 32-33 7.705605195920406E-5 1.232896831347265E-4 0.0 0.007066039964659012 0.0 34-35 9.246726235104487E-5 1.232896831347265E-4 0.0 0.013793033300697526 0.0 36-37 9.246726235104487E-5 1.3099528833064692E-4 0.0 0.023432745400793955 0.0 38-39 9.246726235104487E-5 1.387008935265673E-4 0.0 0.034852452301147994 0.0 40-41 9.246726235104487E-5 1.387008935265673E-4 0.0 0.04669596748727766 0.0 42-43 9.246726235104487E-5 1.387008935265673E-4 0.0 0.05959515058524842 0.0 44-45 9.246726235104487E-5 1.464064987224877E-4 0.0 0.07519900110698724 0.0 46-47 9.246726235104487E-5 1.5411210391840812E-4 0.0 0.09335340694857572 0.0 48-49 9.246726235104487E-5 1.5411210391840812E-4 0.0 0.1126482423591604 0.0 50-51 9.246726235104487E-5 1.6952331431024893E-4 0.0 0.1314036854060307 0.0 52-53 9.246726235104487E-5 1.6952331431024893E-4 0.0 0.1501283060321173 1.5411210391840812E-5 54-55 9.246726235104487E-5 1.6952331431024893E-4 0.0 0.17127248668972286 1.5411210391840812E-5 56-57 9.246726235104487E-5 1.6952331431024893E-4 0.0 0.19732513785712974 1.5411210391840812E-5 58-59 9.246726235104487E-5 1.6952331431024893E-4 0.0 0.22703024588740292 1.5411210391840812E-5 60-61 9.246726235104487E-5 1.8493452470208973E-4 0.0 0.25755985367363954 1.5411210391840812E-5 62-63 9.246726235104487E-5 1.9264012989801015E-4 0.0 0.2892607134496561 1.5411210391840812E-5 64-65 9.246726235104487E-5 2.0034573509393054E-4 0.0 0.3245832076677553 1.5411210391840812E-5 66-67 9.246726235104487E-5 2.1575694548577138E-4 0.0 0.3659854243854356 1.5411210391840812E-5 68-69 1.155840779388061E-4 2.1575694548577138E-4 0.0 0.4112250324906843 1.5411210391840812E-5 70-71 1.232896831347265E-4 2.1575694548577138E-4 0.0 0.4617044521291589 1.5411210391840812E-5 72-73 1.3099528833064692E-4 2.388737610735326E-4 0.0 0.5166223003604836 1.5411210391840812E-5 74-75 1.387008935265673E-4 2.6199057666129383E-4 0.0 0.5727807510283516 1.5411210391840812E-5 76-77 1.387008935265673E-4 2.9281299744497545E-4 0.0 0.6355506109543192 1.5411210391840812E-5 78-79 1.387008935265673E-4 3.159298130327367E-4 0.0 0.7096939441494653 1.5411210391840812E-5 80-81 1.387008935265673E-4 3.2363541822865707E-4 0.0 0.7917124058548421 1.5411210391840812E-5 82-83 1.387008935265673E-4 3.2363541822865707E-4 0.0 0.879232669670106 1.5411210391840812E-5 84-85 1.387008935265673E-4 3.3904662862049785E-4 0.0 0.9694344840935504 1.5411210391840812E-5 86-87 1.387008935265673E-4 3.4675223381641824E-4 0.0 1.0681972258896621 1.5411210391840812E-5 88 1.387008935265673E-4 3.544578390123387E-4 0.0 1.1498766409664185 1.5411210391840812E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6585 0.0 42.634613 1 GTATCAA 11100 0.0 33.850693 1 TCAACGC 11575 0.0 31.062975 4 ATCAACG 11635 0.0 30.984058 3 CAACGCA 12690 0.0 28.297697 5 TATCAAC 13450 0.0 27.082499 2 AACGCAG 13425 0.0 26.959127 6 ACGCAGA 15165 0.0 23.77291 7 GTACATG 18935 0.0 23.594025 1 CGCAGAG 15615 0.0 22.964752 8 GTGGTAT 3925 0.0 22.764477 1 TACATGG 18860 0.0 22.55362 2 ACATGGG 19620 0.0 21.105042 3 GCAGAGT 18080 0.0 18.976099 9 TGGTATC 4365 0.0 18.412859 2 CATGGGG 13280 0.0 17.554409 4 TAGACCG 270 5.451693E-7 17.40827 5 GTACCTG 4545 0.0 17.279306 1 AGAGTAC 16115 0.0 16.434235 10-11 CAGAGTA 17555 0.0 16.304308 10-11 >>END_MODULE