FastQCFastQC Report
Wed 25 May 2016
SRR2049472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6488783
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188780.29093282977717083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177550.2736260405071336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104440.16095468133238544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116000.055.71671
GTATCAA208700.039.7200471
ATCAACG258750.031.7369883
TCAACGC259550.031.6391684
GTGGTAT51550.031.3211921
TATCAAC269400.030.4823552
CAACGCA270300.030.433035
AACGCAG281650.029.2355066
TGGTATC54350.028.8868452
ACGCAGA321100.025.5558417
CGCAGAG325800.025.0429178
GTACATG176500.022.6697651
GCAGAGT358500.022.6406789
TATAACG5900.022.3079282
TACATGG181150.021.5892812
TAACGCA8200.020.6367744
GAGTACT257450.020.61044112-13
AGAGTAC331400.020.54245210-11
ACATGGG183750.020.3372863
CAGAGTA350150.020.27462610-11
Produced by FastQC (version 0.11.5)