##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049472_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6488783 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.879166863801732 33.0 31.0 34.0 30.0 34.0 2 31.96853077071617 33.0 31.0 34.0 30.0 34.0 3 32.012921837577245 33.0 31.0 34.0 30.0 34.0 4 35.75560116588889 37.0 35.0 37.0 33.0 37.0 5 35.82520127426052 37.0 35.0 37.0 35.0 37.0 6 35.91573597082843 37.0 35.0 37.0 35.0 37.0 7 35.91380864485682 37.0 35.0 37.0 35.0 37.0 8 35.9369300838077 37.0 35.0 37.0 35.0 37.0 9 37.65038081871439 39.0 37.0 39.0 35.0 39.0 10-11 37.63514051864579 39.0 37.0 39.0 35.0 39.0 12-13 37.623072693292414 39.0 37.0 39.0 35.0 39.0 14-15 38.93942184535991 40.0 38.0 41.0 36.0 41.0 16-17 38.95426530676092 40.0 38.0 41.0 36.0 41.0 18-19 39.02582364058098 40.0 38.5 41.0 36.0 41.0 20-21 39.04818476746718 40.0 39.0 41.0 36.0 41.0 22-23 39.01931240419043 40.0 39.0 41.0 35.0 41.0 24-25 39.001229121084805 40.0 39.0 41.0 35.0 41.0 26-27 38.90871608743889 40.0 38.0 41.0 35.0 41.0 28-29 38.82695483883495 40.0 38.0 41.0 35.0 41.0 30-31 38.721465720151215 40.0 38.0 41.0 35.0 41.0 32-33 38.592683866913106 40.0 38.0 41.0 35.0 41.0 34-35 38.479151560469816 40.0 38.0 41.0 34.0 41.0 36-37 38.35324844427684 40.0 38.0 41.0 34.0 41.0 38-39 38.251408931382045 40.0 38.0 41.0 34.0 41.0 40-41 38.161570359187536 40.0 38.0 41.0 34.0 41.0 42-43 38.02566968258917 40.0 38.0 41.0 33.5 41.0 44-45 37.855715856116625 40.0 38.0 41.0 33.0 41.0 46-47 37.61400951457307 40.0 37.5 41.0 33.0 41.0 48-49 37.3730458392583 40.0 37.0 41.0 33.0 41.0 50-51 37.21668269689401 40.0 37.0 41.0 32.0 41.0 52-53 37.103447287418916 40.0 37.0 41.0 32.0 41.0 54-55 36.96586093262789 40.0 36.5 41.0 32.0 41.0 56-57 36.81888915070823 40.0 36.0 41.0 31.0 41.0 58-59 36.64608887367631 39.0 36.0 41.0 31.0 41.0 60-61 36.46346102188962 39.0 35.0 41.0 31.0 41.0 62-63 36.248035417427275 39.0 35.0 41.0 31.0 41.0 64-65 36.03032764387405 39.0 35.0 41.0 31.0 41.0 66-67 35.77237896536222 38.0 35.0 40.0 30.0 41.0 68-69 35.481432573719914 37.5 35.0 40.0 30.0 41.0 70-71 35.19554021146955 37.0 35.0 40.0 30.0 41.0 72-73 34.863526257543214 36.5 34.5 39.0 29.0 41.0 74-75 34.539031510223104 36.0 34.0 39.0 29.0 41.0 76-77 34.215736602688054 35.5 34.0 38.5 29.0 40.0 78-79 33.90429692285903 35.0 34.0 37.0 29.0 39.5 80-81 33.59051597194728 35.0 34.0 37.0 29.0 39.0 82-83 33.31266872385777 35.0 34.0 36.5 29.0 39.0 84-85 33.02895758727021 35.0 34.0 36.0 29.0 37.5 86-87 32.780711729765045 35.0 34.0 36.0 29.0 37.0 88-89 32.56109419902006 35.0 34.0 35.5 28.5 37.0 90-91 32.370158009599024 35.0 34.0 35.0 27.5 36.0 92-93 32.206637053512196 35.0 33.5 35.0 27.0 36.0 94-95 32.05008234980273 35.0 33.0 35.0 27.0 36.0 96-97 31.9076465494377 35.0 33.0 35.0 27.0 36.0 98-99 31.775819903362464 35.0 33.0 35.0 27.0 35.5 100 31.66977151185361 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2294581660563857 1101 2 0.28428591217788934 1101 3 0.41746748350723806 1101 4 0.2742640497978144 1101 5 0.2878872139882702 1101 6 0.217771140022613 1101 7 0.23463978582766742 1101 8 0.2524882366645116 1101 9 0.3277253186664666 1101 10-11 0.2848907366015112 1101 12-13 0.25997503329558924 1101 14-15 0.3766008844658728 1101 16-17 0.3681362488649427 1101 18-19 0.3224588803978179 1101 20-21 0.33678613835537163 1101 22-23 0.37230179787894 1101 24-25 0.361527451904756 1101 26-27 0.3784359636190544 1101 28-29 0.3716158257475186 1101 30-31 0.3748188134554127 1101 32-33 0.35139877047938484 1101 34-35 0.3579986972850904 1101 36-37 0.39128180382906663 1101 38-39 0.3520987257689825 1101 40-41 0.35262500514895834 1101 42-43 0.33451791994133373 1101 44-45 0.36650721915003714 1101 46-47 0.35225103934330804 1101 48-49 0.3775419266594824 1101 50-51 0.29622733611135743 1101 52-53 0.2771028260497488 1101 54-55 0.21730969515419218 1101 56-57 0.31195615395063925 1101 58-59 0.28242476667017513 1101 60-61 0.231942988534378 1101 62-63 0.2241337585683425 1101 64-65 0.24033111177345745 1101 66-67 0.2917445135248826 1101 68-69 0.2899911387939653 1101 70-71 0.31676079548327607 1101 72-73 0.30440178567789644 1101 74-75 0.2822159705271119 1101 76-77 0.2910970071550594 1101 78-79 0.252064292220453 1101 80-81 0.24884352068668392 1101 82-83 0.2034467779765592 1101 84-85 0.20639176523173575 1101 86-87 0.1606128782526639 1101 88-89 0.15631526667751672 1101 90-91 0.15069995131462832 1101 92-93 0.23206567580793802 1101 94-95 0.1831349353559517 1101 96-97 0.18252648374948777 1101 98-99 0.27989359843565254 1101 100 0.2916648601367271 1102 1 0.2355930319096906 1102 2 0.22103301499410222 1102 3 0.30369252971533456 1102 4 0.2567885837122148 1102 5 0.23348821220302796 1102 6 0.19208675159175925 1102 7 0.24398771080943504 1102 8 0.2274404140807178 1102 9 0.2606675619835812 1102 10-11 0.2514419455899528 1102 12-13 0.22124708325390685 1102 14-15 0.3293007383710318 1102 16-17 0.353237288935496 1102 18-19 0.3449309821846924 1102 20-21 0.32958640262746997 1102 22-23 0.27581821424303854 1102 24-25 0.2846472330156118 1102 26-27 0.227901961825701 1102 28-29 0.3047235481179271 1102 30-31 0.3425015126686759 1102 32-33 0.32266068647113144 1102 34-35 0.3306512205503225 1102 36-37 0.321354415311518 1102 38-39 0.30095947979984317 1102 40-41 0.36745881888611365 1102 42-43 0.31619832786257973 1102 44-45 0.33153008687975927 1102 46-47 0.31315482019399354 1102 48-49 0.3428954306272587 1102 50-51 0.3169278927714174 1102 52-53 0.29852184478793475 1102 54-55 0.3286179450233675 1102 56-57 0.336348497692029 1102 58-59 0.3365845364450806 1102 60-61 0.25809418934478856 1102 62-63 0.27435590450915015 1102 64-65 0.28515728615396085 1102 66-67 0.2665394967839845 1102 68-69 0.2572709744690087 1102 70-71 0.2753849424061201 1102 72-73 0.3336879088102265 1102 74-75 0.28210480357109446 1102 76-77 0.25946442374881684 1102 78-79 0.25537623580148505 1102 80-81 0.22172598683621203 1102 82-83 0.20855588742012543 1102 84-85 0.17336895435686728 1102 86-87 0.19518206146180717 1102 88-89 0.18111680832996768 1102 90-91 0.18558935509590668 1102 92-93 0.23698850705321917 1102 94-95 0.1545180670395041 1102 96-97 0.1535753871420873 1102 98-99 0.2141482929076055 1102 100 0.20169418339061096 1103 1 0.17585119624124346 1103 2 0.19812878839887915 1103 3 0.28923577787932686 1103 4 0.18810846083908928 1103 5 0.21463884713139691 1103 6 0.19864826062529062 1103 7 0.1951155594821401 1103 8 0.17968377254160828 1103 9 0.23251739744390676 1103 10-11 0.25947307489115445 1103 12-13 0.21673691299953646 1103 14-15 0.33915323850654033 1103 16-17 0.33611323018394046 1103 18-19 0.30109996914195136 1103 20-21 0.3015049226710289 1103 22-23 0.32198804916269097 1103 24-25 0.3257176793417287 1103 26-27 0.3589640635741915 1103 28-29 0.3917833516325757 1103 30-31 0.3748817159258664 1103 32-33 0.36794379341324657 1103 34-35 0.3652514716425799 1103 36-37 0.367740824744061 1103 38-39 0.3402186003656098 1103 40-41 0.3414551618614112 1103 42-43 0.326510492065367 1103 44-45 0.3395591466238912 1103 46-47 0.33076156142954005 1103 48-49 0.3185237089214752 1103 50-51 0.3600931138637975 1103 52-53 0.3888822458559815 1103 54-55 0.3400156011703004 1103 56-57 0.30450106637744057 1103 58-59 0.30438615149775217 1103 60-61 0.23845238778635292 1103 62-63 0.2286747445222943 1103 64-65 0.2092218584099328 1103 66-67 0.22379296870688847 1103 68-69 0.2362377571886043 1103 70-71 0.22788456145318037 1103 72-73 0.26676892339433067 1103 74-75 0.23439358108719688 1103 76-77 0.2537148112162271 1103 78-79 0.24433398746830193 1103 80-81 0.20444807041733526 1103 82-83 0.22980490081285154 1103 84-85 0.24924484063541996 1103 86-87 0.17134914739190776 1103 88-89 0.17830404553097168 1103 90-91 0.2248684051872516 1103 92-93 0.2499241715174989 1103 94-95 0.23339600954344775 1103 96-97 0.27353514926929634 1103 98-99 0.30737762517101785 1103 100 0.28306667112904194 1104 1 0.21118516024089828 1104 2 0.19605240402684032 1104 3 0.28570030717283856 1104 4 0.2392687224812775 1104 5 0.20727608609033865 1104 6 0.18281155012928707 1104 7 0.17068516352228613 1104 8 0.15642886023371005 1104 9 0.23772443808651644 1104 10-11 0.2337659414950437 1104 12-13 0.21793047157061096 1104 14-15 0.2903424625207549 1104 16-17 0.2992961547287578 1104 18-19 0.27245549610689324 1104 20-21 0.299626636342893 1104 22-23 0.2589514381956235 1104 24-25 0.28289441806128224 1104 26-27 0.28543032232224874 1104 28-29 0.28000949217561555 1104 30-31 0.273582431474253 1104 32-33 0.30027282513227505 1104 34-35 0.29642724442240365 1104 36-37 0.3232064967006636 1104 38-39 0.34262273579376057 1104 40-41 0.29018502218384157 1104 42-43 0.29158922297748546 1104 44-45 0.3131666361431229 1104 46-47 0.28870100034461643 1104 48-49 0.25418629407622007 1104 50-51 0.27479124848846936 1104 52-53 0.3096023095486444 1104 54-55 0.25245280071388265 1104 56-57 0.2690009276397518 1104 58-59 0.28430868246528007 1104 60-61 0.2795830527224865 1104 62-63 0.26980333751309615 1104 64-65 0.2701708575405064 1104 66-67 0.3442322761331269 1104 68-69 0.2943624469059358 1104 70-71 0.2904720148124227 1104 72-73 0.3145520381071236 1104 74-75 0.2934687624440997 1104 76-77 0.29915153343318934 1104 78-79 0.2803997200579431 1104 80-81 0.29443440348813255 1104 82-83 0.2873553074944084 1104 84-85 0.24337686123770652 1104 86-87 0.21368499386309736 1104 88-89 0.20371716339111856 1104 90-91 0.20859682819352798 1104 92-93 0.21323269963149727 1104 94-95 0.1850556773047174 1104 96-97 0.17750437317182488 1104 98-99 0.2041572518966639 1104 100 0.20759978717296335 1105 1 0.14852245955178844 1105 2 0.15493292909867407 1105 3 0.2871897017678151 1105 4 0.18378171546681443 1105 5 0.1364756653637187 1105 6 0.12541045445267684 1105 7 0.1160366034868332 1105 8 0.07633077521879983 1105 9 0.15155740895478687 1105 10-11 0.1252368439468725 1105 12-13 0.15115627671381304 1105 14-15 0.17786861264892195 1105 16-17 0.16928056522981905 1105 18-19 0.20453385468454854 1105 20-21 0.20321418664423163 1105 22-23 0.23100510293787835 1105 24-25 0.2241645230449123 1105 26-27 0.20280000745295013 1105 28-29 0.21491768457757132 1105 30-31 0.19601174198184168 1105 32-33 0.19219188321491032 1105 34-35 0.2503087408484248 1105 36-37 0.26101234559236275 1105 38-39 0.2674239081311711 1105 40-41 0.24347260020390848 1105 42-43 0.21039146095842653 1105 44-45 0.19835994562322412 1105 46-47 0.1925699303481352 1105 48-49 0.20130964449112554 1105 50-51 0.18927292307012067 1105 52-53 0.16424945280105163 1105 54-55 0.19790166581555724 1105 56-57 0.17101730303858176 1105 58-59 0.1974491225103705 1105 60-61 0.16585855380893122 1105 62-63 0.1997338552686756 1105 64-65 0.14913888254233143 1105 66-67 0.1574839387598388 1105 68-69 0.1618854098971454 1105 70-71 0.2157060387969949 1105 72-73 0.20735457460096285 1105 74-75 0.20279440904962343 1105 76-77 0.19551070095510426 1105 78-79 0.20816788831504596 1105 80-81 0.21791474660049204 1105 82-83 0.16996136890128355 1105 84-85 0.14251990518687307 1105 86-87 0.14883488581662618 1105 88-89 0.154087679424741 1105 90-91 0.16128295511191482 1105 92-93 0.16917361007582343 1105 94-95 0.14208509068643593 1105 96-97 0.16581456103827819 1105 98-99 0.21827568239326567 1105 100 0.16588536562020195 1106 1 0.105717113290698 1106 2 0.10508009789647232 1106 3 0.21400868122916705 1106 4 0.12815480078591435 1106 5 0.12401286014738844 1106 6 0.10516069980570109 1106 7 0.10761558942439109 1106 8 0.11952713553979066 1106 9 0.11478236621164939 1106 10-11 0.1324303070291677 1106 12-13 0.13706374439630764 1106 14-15 0.1780228244646267 1106 16-17 0.16222784669383827 1106 18-19 0.14678615942129625 1106 20-21 0.20266108555949103 1106 22-23 0.15698643147882052 1106 24-25 0.13930107095404765 1106 26-27 0.1145762014215137 1106 28-29 0.1465575935300052 1106 30-31 0.1820841644123874 1106 32-33 0.17549342875558693 1106 34-35 0.12138468489821719 1106 36-37 0.0651295005122492 1106 38-39 0.039887443072764484 1106 40-41 0.01243902941168784 1106 42-43 0.05041302888410826 1106 44-45 0.06393780249403136 1106 46-47 0.07477503941984054 1106 48-49 0.07640370443375133 1106 50-51 0.03176137819553304 1106 52-53 0.010121380128005342 1106 54-55 0.08487224150845663 1106 56-57 0.06729255964218339 1106 58-59 0.07402695347239785 1106 60-61 0.036296710701606116 1106 62-63 0.07055352964555084 1106 64-65 0.02139450865405479 1106 66-67 0.0016835974147468846 1106 68-69 -0.013070462184714415 1106 70-71 0.019620991656204012 1106 72-73 0.09610024890245228 1106 74-75 0.06067987414784426 1106 76-77 0.07799317400989025 1106 78-79 0.06063893205897841 1106 80-81 -0.017074925570106814 1106 82-83 0.007998224196150261 1106 84-85 0.04566107573347011 1106 86-87 0.026966526422654624 1106 88-89 0.020046993116373812 1106 90-91 0.0246809397576655 1106 92-93 -0.010748877351112185 1106 94-95 0.0027397671891122855 1106 96-97 0.017169784710265645 1106 98-99 -0.005880619374995177 1106 100 -0.007655676611225459 1107 1 0.087416205519812 1107 2 0.05563896116433753 1107 3 0.15422909042796817 1107 4 0.0999370937161288 1107 5 0.09664560184856441 1107 6 0.08998292093908589 1107 7 0.0872778108446326 1107 8 0.09889940757535243 1107 9 0.07968575814278012 1107 10-11 0.0922196211404156 1107 12-13 0.0994974563715374 1107 14-15 0.14231853904106373 1107 16-17 0.18795267399209337 1107 18-19 0.157137464105503 1107 20-21 0.16978172928148183 1107 22-23 0.1663735642792048 1107 24-25 0.17646781972008085 1107 26-27 0.15777465532826085 1107 28-29 0.2057920852852746 1107 30-31 0.185811964679516 1107 32-33 0.14125939004622978 1107 34-35 0.14509791855830656 1107 36-37 0.14556235947286922 1107 38-39 0.16339601485174882 1107 40-41 0.16809219644095919 1107 42-43 0.17003002215108154 1107 44-45 0.1854101583409431 1107 46-47 0.15446220842525094 1107 48-49 0.17091246374368296 1107 50-51 0.20036971519367341 1107 52-53 0.1572565539557118 1107 54-55 0.1701617043518837 1107 56-57 0.12539235389062497 1107 58-59 0.13187543069919627 1107 60-61 0.183607364671019 1107 62-63 0.1667038718369156 1107 64-65 0.14068296848388684 1107 66-67 0.13881933400706714 1107 68-69 0.1098659780931257 1107 70-71 0.1404399092904356 1107 72-73 0.15634738416002136 1107 74-75 0.16212629622954466 1107 76-77 0.14427214323639248 1107 78-79 0.1905603204338533 1107 80-81 0.16610836010179497 1107 82-83 0.1333759324391366 1107 84-85 0.11159496783202627 1107 86-87 0.11813876361448195 1107 88-89 0.11617563312020707 1107 90-91 0.14438868713908448 1107 92-93 0.18178922134101327 1107 94-95 0.19353825545864112 1107 96-97 0.15444126330568864 1107 98-99 0.16429850402883162 1107 100 0.15162012928123048 1108 1 0.043000299345106185 1108 2 0.04112111405545349 1108 3 0.09213916791100729 1108 4 0.045132158922548626 1108 5 0.07345271301075229 1108 6 0.08257005916852478 1108 7 0.06245345414969705 1108 8 0.07803898882391991 1108 9 0.09513660168724414 1108 10-11 0.0415354346056489 1108 12-13 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-0.29898068814114254 2214 94-95 -0.23656353404157926 2214 96-97 -0.20828051991939844 2214 98-99 -0.24591366246886892 2214 100 -0.1912247534953515 2215 1 -0.37780560016003406 2215 2 -0.3390277075093593 2215 3 -0.23453082586884122 2215 4 -0.25575148194225505 2215 5 -0.2571909240424972 2215 6 -0.2494834574177034 2215 7 -0.25494898657001386 2215 8 -0.25887710660970953 2215 9 -0.34448262265304663 2215 10-11 -0.4035975085606438 2215 12-13 -0.42118862680631963 2215 14-15 -0.5545170204649992 2215 16-17 -0.5696398582502198 2215 18-19 -0.5032650106630001 2215 20-21 -0.515604570078608 2215 22-23 -0.4765489324963781 2215 24-25 -0.4935025439713385 2215 26-27 -0.4613402619482301 2215 28-29 -0.3920543651188737 2215 30-31 -0.4579938448698826 2215 32-33 -0.4604246587557128 2215 34-35 -0.49160331794786316 2215 36-37 -0.5060792406419239 2215 38-39 -0.5262833219452148 2215 40-41 -0.5869110787813199 2215 42-43 -0.546610001190686 2215 44-45 -0.5502932643227894 2215 46-47 -0.5709073297245055 2215 48-49 -0.5330159088634687 2215 50-51 -0.49723105590730654 2215 52-53 -0.5845605333468384 2215 54-55 -0.5016346490750863 2215 56-57 -0.47277434325781087 2215 58-59 -0.47756083467935184 2215 60-61 -0.4872657548985515 2215 62-63 -0.4577934611963599 2215 64-65 -0.45278649417645056 2215 66-67 -0.3835627837057558 2215 68-69 -0.40655513470974824 2215 70-71 -0.39837323093649246 2215 72-73 -0.3828599806872006 2215 74-75 -0.30352554701437384 2215 76-77 -0.36476498029806237 2215 78-79 -0.3374495724685147 2215 80-81 -0.40961895625557787 2215 82-83 -0.41265279468480287 2215 84-85 -0.3758177232215658 2215 86-87 -0.3603155798370423 2215 88-89 -0.3961547759702384 2215 90-91 -0.40527398754466404 2215 92-93 -0.3823034981822957 2215 94-95 -0.3840008132643291 2215 96-97 -0.3461833934607981 2215 98-99 -0.36410006280113194 2215 100 -0.39101044496270276 2216 1 -0.26702255924798735 2216 2 -0.2489565261555562 2216 3 -0.19400152001748694 2216 4 -0.16997487789254961 2216 5 -0.2723284315074892 2216 6 -0.25039048283028364 2216 7 -0.2986004827046358 2216 8 -0.28995254065951315 2216 9 -0.4049162198885128 2216 10-11 -0.39924309410607606 2216 12-13 -0.4539871964050093 2216 14-15 -0.5696026791964783 2216 16-17 -0.5742540843892954 2216 18-19 -0.5674732651622634 2216 20-21 -0.548002760074084 2216 22-23 -0.5206544985004342 2216 24-25 -0.5936977594638435 2216 26-27 -0.5719878109148624 2216 28-29 -0.6075817102263059 2216 30-31 -0.6050329746615191 2216 32-33 -0.5770745153762888 2216 34-35 -0.5951404739311954 2216 36-37 -0.6322875420731506 2216 38-39 -0.6027916510240345 2216 40-41 -0.6439343564133182 2216 42-43 -0.7324330690283318 2216 44-45 -0.7315927354801772 2216 46-47 -0.657112761259043 2216 48-49 -0.6149708223972823 2216 50-51 -0.5597712942111883 2216 52-53 -0.6206388763309221 2216 54-55 -0.6538096797481785 2216 56-57 -0.6046456074292124 2216 58-59 -0.5982883078301171 2216 60-61 -0.5977409740295485 2216 62-63 -0.622221533196921 2216 64-65 -0.6703415585553074 2216 66-67 -0.6401992027091268 2216 68-69 -0.6523676466937331 2216 70-71 -0.6622071412701445 2216 72-73 -0.6045218583919549 2216 74-75 -0.6154210770153412 2216 76-77 -0.5719801568547069 2216 78-79 -0.5530265304210076 2216 80-81 -0.5650667632133377 2216 82-83 -0.5418136483128535 2216 84-85 -0.6005517297396707 2216 86-87 -0.5757157615026216 2216 88-89 -0.6038419673909488 2216 90-91 -0.5165035730058065 2216 92-93 -0.5524001877671125 2216 94-95 -0.551888112214634 2216 96-97 -0.5558409887083613 2216 98-99 -0.63443248883409 2216 100 -0.6413635615744795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 479.0 3 26.0 4 59.0 5 127.0 6 404.0 7 1086.0 8 2396.0 9 4294.0 10 5658.0 11 7008.0 12 10073.0 13 14480.0 14 16927.0 15 21423.0 16 27570.0 17 39606.0 18 39625.0 19 20797.0 20 14601.0 21 14142.0 22 14414.0 23 15795.0 24 18317.0 25 22943.0 26 29558.0 27 38407.0 28 50521.0 29 66700.0 30 87916.0 31 114032.0 32 148453.0 33 196827.0 34 273236.0 35 404507.0 36 677475.0 37 1312051.0 38 1954982.0 39 819371.0 40 2497.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.403970522115046 20.82803713342042 14.801756255379134 22.966236089085402 2 15.411641905731784 21.260150015804196 39.6073500993946 23.720857979069418 3 17.831078647567654 25.97789138579607 31.03737634622702 25.15365362040925 4 12.713662947273779 17.964616785612957 38.56273202540446 30.758988241708806 5 12.519954235307154 37.780193530458135 35.595119028737955 14.104733205496759 6 29.548560259389568 38.72536456430207 17.810393428773054 13.915681747535318 7 25.764507766710643 33.200108556566 23.005346302997033 18.03003737372632 8 24.53161093536338 35.857571442903854 20.85671535016659 18.75410227156618 9 24.51217739905927 16.833341475589492 22.19476287001738 36.459718255333854 10-11 23.212072279193187 27.275815819391706 29.223176364504717 20.288935536910387 12-13 24.209616811041453 24.90298257778076 29.423198464180416 21.464202146997362 14-15 21.4728247192116 26.890620321252847 27.300412111177085 24.336142848358467 16-17 20.37524602070989 30.080740872363894 27.93600125015739 21.608011856768826 18-19 20.204096823703306 28.331807366651034 30.992953532272537 20.471142277373122 20-21 21.28713504520031 27.632323349386162 30.76653973480081 20.314001870612717 22-23 20.987063367660777 27.45967155936637 30.876113440686797 20.677151632286055 24-25 20.81963597796382 27.591237062481515 30.817311967436726 20.77181499211794 26-27 20.673036839111433 27.524629811167976 30.870927568389945 20.931405781330646 28-29 20.672520563563307 27.508548213124094 31.02396859318612 20.79496263012648 30-31 20.94427414200783 27.833593757103603 30.624348202120487 20.597783898768075 32-33 20.597298445640728 27.66872462833169 30.965120886304874 20.768856039722703 34-35 20.808193154247874 27.400924025352673 30.871320554254932 20.919562266144514 36-37 21.133847441037865 27.687117907934354 30.378932382235618 20.800102268792163 38-39 20.996209921028335 27.78955622340892 30.464356721437596 20.74987713412515 40-41 20.883731201983487 27.637647922576548 30.487758644417607 20.990862231022366 42-43 20.888107985734766 27.71583669849955 30.288645806155024 21.10740950961066 44-45 21.118505581092787 27.817334930140213 30.050357671076377 21.013801817690624 46-47 21.227717123534568 28.011300424131917 29.60406442933906 21.15691802299445 48-49 21.25056424294047 27.980593587426178 29.41839016653816 21.35045200309519 50-51 21.361864004390345 27.993862639573553 29.191444682307914 21.452828673728185 52-53 21.312224495718226 28.121074475753005 29.090431903794595 21.476269124734177 54-55 21.432162240592728 28.415482533473536 28.929423283225837 21.222931942707902 56-57 21.297892070053813 28.14106281563122 28.990967951925654 21.57007716238931 58-59 21.169894262144382 27.82551057725308 29.31205281483446 21.692542345768075 60-61 21.387115272617375 28.156581904495802 29.013383557440587 21.442919265446232 62-63 21.21532651037953 28.131530982003866 29.144902826924557 21.50823968069205 64-65 21.22359462475475 27.916621653089646 29.265102562375716 21.594681159779885 66-67 21.1734388405345 27.988946463458557 29.139655309786132 21.697959386220806 68-69 21.53208082316823 28.104831060000002 28.97370739628679 21.389380720544978 70-71 21.513710660381154 27.915119060076442 29.01003932478556 21.561130954756848 72-73 21.337768576942704 28.03035638578143 29.132912905239706 21.498962132036162 74-75 21.453685649041248 28.082355973558986 28.9168422581586 21.547116119241167 76-77 21.493606736424997 28.11135000199575 28.772036297099163 21.623006964480087 78-79 21.573429100649534 28.138057629604813 28.886002197946826 21.402511071798827 80-81 21.44521170991894 28.04815548703549 28.91603149902389 21.59060130402168 82-83 21.334916382501483 28.10834640019289 29.0257736339863 21.53096358331933 84-85 21.372686315879992 28.06101506166395 28.876918442873162 21.68938017958289 86-87 21.43997963244547 28.186924711171322 28.787286737672108 21.5858089187111 88-89 21.275021331187872 28.072891209901584 28.866404463671948 21.7856829952386 90-91 21.246576756930864 28.021808581956716 28.88365674022497 21.847957920887445 92-93 21.32104604773141 28.195680319542987 28.760988027463462 21.72228560526214 94-95 21.17687545082721 28.016110612086287 28.917568539413463 21.88944539767304 96-97 21.156718286818172 28.07320984258127 28.907993764587758 21.8620781060128 98-99 21.141021205309844 28.116357578284113 28.81632775723353 21.926293459172516 100 21.271974672576974 28.031969660846723 28.760365782334635 21.93568988424167 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1565.0 1 1111.0 2 1356.0 3 1997.0 4 1287.0 5 773.5 6 1726.0 7 4332.0 8 7216.5 9 8209.5 10 6324.0 11 4054.5 12 3706.0 13 4122.5 14 4649.0 15 5229.5 16 5970.0 17 6831.5 18 7764.5 19 8946.0 20 10682.5 21 13050.5 22 16487.0 23 21891.5 24 29371.5 25 38750.0 26 51571.5 27 67402.5 28 86324.5 29 107696.0 30 127107.5 31 144952.5 32 161924.5 33 177061.0 34 190646.0 35 201925.0 36 211705.0 37 220510.5 38 228695.5 39 233284.5 40 234559.5 41 234813.0 42 232018.0 43 228138.5 44 223490.0 45 218575.0 46 213317.5 47 205910.0 48 199226.0 49 194769.5 50 189469.0 51 180016.5 52 166634.0 53 155460.5 54 147981.0 55 140254.5 56 130174.5 57 117798.0 58 106988.0 59 98060.5 60 86373.0 61 72573.0 62 60646.0 63 50141.0 64 40596.5 65 32710.0 66 25783.5 67 19670.5 68 15193.0 69 11664.5 70 8576.5 71 6280.0 72 4519.0 73 3155.0 74 2167.5 75 1442.0 76 867.0 77 540.5 78 354.0 79 203.5 80 112.0 81 54.0 82 26.5 83 14.0 84 10.0 85 6.0 86 4.5 87 2.5 88 1.5 89 2.0 90 0.5 91 0.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 1.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11898995543540292 2 0.0 3 0.0 4 0.0 5 0.012513902838174738 6 1.5411210391840812E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.705605195920406E-6 76-77 0.0 78-79 0.0 80-81 1.618177091143285E-4 82-83 6.087428104777121E-4 84-85 3.7757465460009986E-4 86-87 4.854531273429856E-4 88-89 6.857988624369161E-4 90-91 8.16794150767563E-4 92-93 2.46579366269453E-4 94-95 2.542849714653734E-4 96-97 5.856259948899508E-4 98-99 0.0012020744105635834 100 9.246726235104488E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6488783.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.29148018653574 #Duplication Level Percentage of deduplicated Percentage of total 1 75.34876043683094 46.182370573940524 2 15.370516455759677 18.841634096100318 3 4.462539669081276 8.205469851273744 4 1.788970549872905 4.385946120473244 5 0.8927008833669533 2.735747925269429 6 0.5260971828439774 1.9347165035084353 7 0.31823936884821347 1.3653753379235138 8 0.2264839578439582 1.1105229611808929 9 0.16951355984725153 0.9350763295254262 >10 0.8309055413559956 9.022941801485674 >50 0.045560612822862924 1.8965963426589423 >100 0.01865691725470791 1.9841994310591082 >500 6.530256873483187E-4 0.2647163295361307 >1k 3.013789381485092E-4 0.32806203099321224 >5k 2.5114911470789754E-5 0.08195393446003266 >10k+ 7.534473441236927E-5 0.7246704306113744 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18878 0.29093282977717083 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17755 0.2736260405071336 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10444 0.16095468133238544 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5411210391840812E-5 0.0 5 0.0 0.0 0.0 1.5411210391840812E-5 0.0 6 0.0 0.0 0.0 1.5411210391840812E-5 0.0 7 0.0 0.0 0.0 3.0822420783681625E-5 0.0 8 0.0 0.0 0.0 3.0822420783681625E-5 0.0 9 1.5411210391840812E-5 3.0822420783681625E-5 0.0 3.0822420783681625E-5 0.0 10-11 1.5411210391840812E-5 3.0822420783681625E-5 0.0 3.0822420783681625E-5 0.0 12-13 1.5411210391840812E-5 3.0822420783681625E-5 0.0 3.852802597960203E-5 0.0 14-15 1.5411210391840812E-5 4.623363117552244E-5 0.0 1.0017286754696527E-4 0.0 16-17 1.5411210391840812E-5 6.164484156736325E-5 0.0 1.6181770911432854E-4 0.0 18-19 2.311681558776122E-5 6.164484156736325E-5 0.0 1.6952331431024893E-4 0.0 20-21 3.0822420783681625E-5 6.164484156736325E-5 0.0 1.8493452470208973E-4 0.0 22-23 3.0822420783681625E-5 6.164484156736325E-5 0.0 2.696961818572142E-4 0.0 24-25 3.0822420783681625E-5 6.935044676328365E-5 0.0 3.4675223381641824E-4 0.0 26-27 3.0822420783681625E-5 8.476165715512446E-5 0.0 5.393923637144285E-4 0.0 28-29 3.852802597960203E-5 9.246726235104487E-5 0.0 0.0013253640936983098 0.0 30-31 4.6233631175522434E-5 9.246726235104487E-5 0.0 0.0029512467900375157 0.0 32-33 4.6233631175522434E-5 9.246726235104487E-5 0.0 0.006881105439956923 0.0 34-35 4.6233631175522434E-5 9.246726235104487E-5 0.0 0.013754505274717924 0.0 36-37 4.6233631175522434E-5 1.0017286754696527E-4 0.0 0.023371100559226594 0.0 38-39 4.6233631175522434E-5 1.0787847274288569E-4 0.0 0.03472145701281735 0.0 40-41 4.6233631175522434E-5 1.0787847274288569E-4 0.0 0.046564972198947015 0.0 42-43 4.6233631175522434E-5 1.232896831347265E-4 0.0 0.05957203376966066 0.0 44-45 4.6233631175522434E-5 1.232896831347265E-4 0.0 0.07520670671218316 0.0 46-47 4.6233631175522434E-5 1.232896831347265E-4 0.0 0.09353063586808189 0.0 48-49 4.6233631175522434E-5 1.232896831347265E-4 0.0 0.11287170490984211 0.0 50-51 4.6233631175522434E-5 1.5411210391840812E-4 0.0 0.13171961521906342 0.0 52-53 4.6233631175522434E-5 1.5411210391840812E-4 0.0 0.1505521143178929 0.0 54-55 4.6233631175522434E-5 1.5411210391840812E-4 0.0 0.1716962949754985 0.0 56-57 4.6233631175522434E-5 1.5411210391840812E-4 0.0 0.1978414134052564 0.0 58-59 4.6233631175522434E-5 1.6952331431024893E-4 0.0 0.2276698111186643 0.0 60-61 4.6233631175522434E-5 1.8493452470208973E-4 0.0 0.25836894221921125 0.0 62-63 4.6233631175522434E-5 1.8493452470208973E-4 0.0 0.29008521320561964 0.0 64-65 4.6233631175522434E-5 2.0034573509393054E-4 0.0 0.3254693522652861 0.0 66-67 4.6233631175522434E-5 2.0034573509393054E-4 0.0 0.36681762974659504 0.0 68-69 4.6233631175522434E-5 2.0034573509393054E-4 0.0 0.4119493593791008 0.0 70-71 4.6233631175522434E-5 2.2346255068169177E-4 0.0 0.4624364846227713 0.0 72-73 4.6233631175522434E-5 2.619905766612938E-4 0.0 0.5174236833008593 0.0 74-75 7.705605195920406E-5 2.774017870531346E-4 0.0 0.5736360732050987 0.0 76-77 7.705605195920406E-5 2.774017870531346E-4 0.0 0.6366910405233154 0.0 78-79 7.705605195920406E-5 2.9281299744497545E-4 0.0 0.7107727288768941 0.0 80-81 7.705605195920406E-5 2.9281299744497545E-4 0.0 0.7927757793718792 0.0 82-83 7.705605195920406E-5 3.0051860264089584E-4 0.0 0.8806042673949799 0.0 84-85 7.705605195920406E-5 3.159298130327367E-4 0.0 0.9712453013145916 0.0 86-87 7.705605195920406E-5 3.2363541822865707E-4 0.0 1.0703933233704994 0.0 88 7.705605195920406E-5 3.2363541822865707E-4 0.0 1.1523116122083294 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11600 0.0 55.7167 1 GTATCAA 20870 0.0 39.720047 1 ATCAACG 25875 0.0 31.736988 3 TCAACGC 25955 0.0 31.639168 4 GTGGTAT 5155 0.0 31.321192 1 TATCAAC 26940 0.0 30.482355 2 CAACGCA 27030 0.0 30.43303 5 AACGCAG 28165 0.0 29.235506 6 TGGTATC 5435 0.0 28.886845 2 ACGCAGA 32110 0.0 25.555841 7 CGCAGAG 32580 0.0 25.042917 8 GTACATG 17650 0.0 22.669765 1 GCAGAGT 35850 0.0 22.640678 9 TATAACG 590 0.0 22.307928 2 TACATGG 18115 0.0 21.589281 2 TAACGCA 820 0.0 20.636774 4 GAGTACT 25745 0.0 20.610441 12-13 AGAGTAC 33140 0.0 20.542452 10-11 ACATGGG 18375 0.0 20.337286 3 CAGAGTA 35015 0.0 20.274626 10-11 >>END_MODULE