FastQCFastQC Report
Wed 25 May 2016
SRR2049471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7657980
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84000.045.183361
GTATCAA140150.035.269061
TCAACGC149950.031.6244164
ATCAACG150600.031.5199553
TATCAAC169400.028.5490152
CAACGCA168300.028.1776475
AACGCAG177550.026.8421826
GTACATG257800.024.5006371
ACGCAGA200050.023.518077
TACATGG265000.022.7191682
CGCAGAG205100.022.5710248
ACATGGG267550.021.7472763
GTGGTAT45750.020.0460031
GCAGAGT227450.019.630049
CATGGGG191300.018.6468124
CAGAGTA212400.017.72416310-11
GTATAGG27750.017.6260341
AGAGTAC199300.016.73142210-11
GAGTACA194650.016.5025311
TGGTATC55200.015.581212
Produced by FastQC (version 0.11.5)