##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049471_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7657980
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87370755734541	31.0	31.0	34.0	28.0	34.0
2	31.103688570615226	31.0	31.0	34.0	28.0	34.0
3	31.185878913238216	31.0	31.0	34.0	28.0	34.0
4	34.77345043993325	37.0	35.0	37.0	32.0	37.0
5	34.74397856876095	37.0	35.0	37.0	32.0	37.0
6	34.82229883076216	37.0	35.0	37.0	32.0	37.0
7	34.73634169846356	37.0	35.0	37.0	32.0	37.0
8	34.73765222682744	37.0	35.0	37.0	32.0	37.0
9	36.29025434905811	39.0	37.0	39.0	32.0	39.0
10-11	36.22423152058376	39.0	37.0	39.0	32.0	39.0
12-13	36.14110529147373	39.0	37.0	39.0	32.0	39.0
14-15	37.374089707729716	40.0	38.0	41.0	32.0	41.0
16-17	37.35945262327663	40.0	38.0	41.0	32.0	41.0
18-19	37.37280385950342	40.0	38.0	41.0	32.0	41.0
20-21	37.41568892841193	40.0	38.0	41.0	32.0	41.0
22-23	37.37667061026537	40.0	38.0	41.0	31.5	41.0
24-25	37.37529015484501	40.0	38.0	41.0	31.5	41.0
26-27	37.364247817309526	40.0	38.0	41.0	31.5	41.0
28-29	37.34108080721026	40.0	38.0	41.0	31.0	41.0
30-31	37.283056497927646	40.0	38.0	41.0	31.0	41.0
32-33	37.23800989294827	40.0	37.0	41.0	31.0	41.0
34-35	37.16576518873123	40.0	37.0	41.0	31.0	41.0
36-37	37.0618920394151	40.0	37.0	41.0	31.0	41.0
38-39	36.95998899187514	40.0	37.0	41.0	30.0	41.0
40-41	36.836309966858096	40.0	37.0	41.0	30.0	41.0
42-43	36.740921104521036	40.0	37.0	41.0	30.0	41.0
44-45	36.58631120739412	40.0	36.0	41.0	30.0	41.0
46-47	36.4592674569534	40.0	36.0	41.0	30.0	41.0
48-49	36.34679177798846	40.0	36.0	41.0	30.0	41.0
50-51	36.21638637081841	39.5	35.5	41.0	29.5	41.0
52-53	36.085815841775506	39.0	35.0	41.0	29.0	41.0
54-55	35.91933656133863	39.0	35.0	41.0	29.0	41.0
56-57	35.73240142961983	39.0	35.0	41.0	28.5	41.0
58-59	35.52408330917552	39.0	35.0	41.0	28.0	41.0
60-61	35.30863223722183	38.0	35.0	40.0	28.0	41.0
62-63	35.03503241063571	38.0	34.0	40.0	28.0	41.0
64-65	34.7760035283456	37.5	34.0	40.0	28.0	41.0
66-67	34.53103181256676	37.0	34.0	40.0	27.0	41.0
68-69	34.23381936228614	36.5	34.0	39.5	26.5	41.0
70-71	33.917240904259344	36.0	34.0	39.0	26.0	41.0
72-73	33.57847134622969	35.5	33.5	39.0	26.0	41.0
74-75	33.273944434433105	35.0	33.0	38.5	26.0	40.0
76-77	32.95282006482127	35.0	33.0	37.0	26.0	39.5
78-79	32.61394976220883	35.0	33.0	37.0	25.0	39.0
80-81	32.29323960104362	35.0	33.0	36.5	25.0	39.0
82-83	32.007341492142835	35.0	33.0	36.0	24.5	38.0
84-85	31.738051810007338	35.0	33.0	36.0	24.0	37.0
86-87	31.480813021188354	35.0	32.0	35.0	24.0	37.0
88-89	31.258632171930458	35.0	32.0	35.0	23.0	36.0
90-91	31.05107933162531	35.0	32.0	35.0	21.0	36.0
92-93	30.844599359099917	35.0	32.0	35.0	20.0	36.0
94-95	30.637308402476894	35.0	32.0	35.0	18.5	36.0
96-97	30.435672070180388	35.0	32.0	35.0	13.0	35.0
98-99	30.241217919085713	35.0	32.0	35.0	2.0	35.0
100	30.088310233246887	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3067657821469645
1101	2	0.3886143911755795
1101	3	0.39852799379452364
1101	4	0.4591692177600919
1101	5	0.43356795990469266
1101	6	0.4507076518610731
1101	7	0.5265712717733066
1101	8	0.5581796365831408
1101	9	0.6240051176476342
1101	10-11	0.6365758972692745
1101	12-13	0.5949298203270104
1101	14-15	0.7437471868527794
1101	16-17	0.6594882283310284
1101	18-19	0.6889564540527147
1101	20-21	0.6888568571069484
1101	22-23	0.7020090449141989
1101	24-25	0.7321464747561919
1101	26-27	0.736903893639429
1101	28-29	0.7019060748157528
1101	30-31	0.6885869101065296
1101	32-33	0.7509813878263145
1101	34-35	0.7092935999513585
1101	36-37	0.645564965105585
1101	38-39	0.693115941592545
1101	40-41	0.7087444955249111
1101	42-43	0.6427698500945738
1101	44-45	0.6856988329804636
1101	46-47	0.6525222846860501
1101	48-49	0.6161252798116053
1101	50-51	0.5841200883689126
1101	52-53	0.5963635507145426
1101	54-55	0.5366228252542058
1101	56-57	0.5624095496195665
1101	58-59	0.5619862581538797
1101	60-61	0.570816777928087
1101	62-63	0.5038783727555014
1101	64-65	0.5209864378120912
1101	66-67	0.4971079727837804
1101	68-69	0.463875652493158
1101	70-71	0.4805855752044508
1101	72-73	0.46938638645774944
1101	74-75	0.4905200563070693
1101	76-77	0.440200763427093
1101	78-79	0.42709432382849144
1101	80-81	0.47499678761418807
1101	82-83	0.4689339488486084
1101	84-85	0.4183824478717604
1101	86-87	0.3970107518841637
1101	88-89	0.37867418778854045
1101	90-91	0.4384060080185108
1101	92-93	0.3875670694482345
1101	94-95	0.4069762833640027
1101	96-97	0.4638476359859993
1101	98-99	0.5236647927245386
1101	100	0.5020996117834677
1102	1	0.187418118729326
1102	2	0.275680656073348
1102	3	0.30825700972889436
1102	4	0.3611185202195273
1102	5	0.29486723896413025
1102	6	0.3221013208671266
1102	7	0.29668090236597777
1102	8	0.4009350122228099
1102	9	0.48027261209492167
1102	10-11	0.4691870602782089
1102	12-13	0.4165344034469527
1102	14-15	0.477580501643871
1102	16-17	0.4686342288318244
1102	18-19	0.4689512327273704
1102	20-21	0.4509249800594475
1102	22-23	0.45982815106498265
1102	24-25	0.43322059895730547
1102	26-27	0.3890670447899254
1102	28-29	0.47556815281512854
1102	30-31	0.36507837638678353
1102	32-33	0.39400775595989757
1102	34-35	0.4551083603994428
1102	36-37	0.40155107153005787
1102	38-39	0.42060299382434607
1102	40-41	0.4278106617350659
1102	42-43	0.4223138302117846
1102	44-45	0.35518663011981033
1102	46-47	0.3021768225077821
1102	48-49	0.3013104979863144
1102	50-51	0.368325228644764
1102	52-53	0.2968690431243317
1102	54-55	0.3066150344498695
1102	56-57	0.3077562430943246
1102	58-59	0.3431330307626155
1102	60-61	0.37114518814427555
1102	62-63	0.3249159139660094
1102	64-65	0.3109386275736412
1102	66-67	0.35380947625270665
1102	68-69	0.31640879214440076
1102	70-71	0.29480078000729293
1102	72-73	0.3043381046181963
1102	74-75	0.35735029500235527
1102	76-77	0.33210409071657665
1102	78-79	0.3455989322639539
1102	80-81	0.35870776961412076
1102	82-83	0.3403525483519232
1102	84-85	0.2675997547666249
1102	86-87	0.21806308091171545
1102	88-89	0.2608661621567343
1102	90-91	0.25109624792134255
1102	92-93	0.24084117067842925
1102	94-95	0.21464283172813126
1102	96-97	0.2390890030423023
1102	98-99	0.28705480668791594
1102	100	0.21991201246373748
1103	1	0.2652138747069692
1103	2	0.31905918419913704
1103	3	0.3491377419423429
1103	4	0.3296940147596459
1103	5	0.26177703808926367
1103	6	0.31444395695450567
1103	7	0.3166155757884894
1103	8	0.3756514168167655
1103	9	0.39320860738684615
1103	10-11	0.43599517503860596
1103	12-13	0.43566359345513206
1103	14-15	0.5523554237262616
1103	16-17	0.5247016425676918
1103	18-19	0.4941417230167673
1103	20-21	0.5038104905541445
1103	22-23	0.484004658975131
1103	24-25	0.5067490469603086
1103	26-27	0.5199747432704456
1103	28-29	0.49856420083894193
1103	30-31	0.52029714894433
1103	32-33	0.5276422702594559
1103	34-35	0.5107832154443273
1103	36-37	0.525892118757092
1103	38-39	0.5955695233664073
1103	40-41	0.6082530433044582
1103	42-43	0.548071460284298
1103	44-45	0.5373201168049633
1103	46-47	0.5007110796139571
1103	48-49	0.5607188349487799
1103	50-51	0.5408216409426316
1103	52-53	0.5265203104947815
1103	54-55	0.4850666750827628
1103	56-57	0.48462940687097245
1103	58-59	0.48602774305901164
1103	60-61	0.4751511848883325
1103	62-63	0.5273039620227706
1103	64-65	0.5290207948460761
1103	66-67	0.44498584762386884
1103	68-69	0.46529411590946523
1103	70-71	0.49110991822687566
1103	72-73	0.4827729785846202
1103	74-75	0.4677956484633654
1103	76-77	0.387037198460483
1103	78-79	0.41036329790733106
1103	80-81	0.48944009240831576
1103	82-83	0.4804527767503757
1103	84-85	0.4115446207914779
1103	86-87	0.42589398984582516
1103	88-89	0.45648548417980805
1103	90-91	0.4516549433186867
1103	92-93	0.38174521493688474
1103	94-95	0.4303107311578742
1103	96-97	0.4461187067957155
1103	98-99	0.40805270819380723
1103	100	0.422604662554928
1104	1	0.17480485902750686
1104	2	0.23744591323865905
1104	3	0.2681769157731715
1104	4	0.2947857335308015
1104	5	0.2674590117737168
1104	6	0.31538683993951366
1104	7	0.3457862979042474
1104	8	0.3050341817580602
1104	9	0.42784029483015473
1104	10-11	0.4379248379125542
1104	12-13	0.47329275544115745
1104	14-15	0.47735291826933945
1104	16-17	0.44421945715643574
1104	18-19	0.4937183516644197
1104	20-21	0.43293144120004
1104	22-23	0.43546517260614337
1104	24-25	0.4345260380474514
1104	26-27	0.43520538015780375
1104	28-29	0.3905151883439544
1104	30-31	0.42891481249568386
1104	32-33	0.4474382197873723
1104	34-35	0.4498935819146439
1104	36-37	0.47191189686646595
1104	38-39	0.46737580606965423
1104	40-41	0.4719161299780268
1104	42-43	0.47697950661709143
1104	44-45	0.4889185550275812
1104	46-47	0.41873826097514666
1104	48-49	0.42916542005753655
1104	50-51	0.40339640475109917
1104	52-53	0.38616831100596016
1104	54-55	0.3925067803764577
1104	56-57	0.35440012866637716
1104	58-59	0.4208885602193604
1104	60-61	0.41420764128522336
1104	62-63	0.40003475508181197
1104	64-65	0.3875648228562838
1104	66-67	0.4093041367147663
1104	68-69	0.37484925109179557
1104	70-71	0.327155341392384
1104	72-73	0.31447533558429086
1104	74-75	0.3422764474424298
1104	76-77	0.3156602742316963
1104	78-79	0.32807307954447396
1104	80-81	0.3046547852821533
1104	82-83	0.3520553371260391
1104	84-85	0.2570833511726711
1104	86-87	0.25120915684518863
1104	88-89	0.2648081033929124
1104	90-91	0.3008728273329382
1104	92-93	0.32738413329120064
1104	94-95	0.3551598896641863
1104	96-97	0.40726846194926125
1104	98-99	0.434770376944396
1104	100	0.3659332401017288
1105	1	0.1223782194219929
1105	2	0.18296741256303406
1105	3	0.14398013032492685
1105	4	0.1730281224360155
1105	5	0.12173260164794186
1105	6	0.17115533928783577
1105	7	0.22893851212472072
1105	8	0.1864893704721311
1105	9	0.22980826277189692
1105	10-11	0.2763808731496695
1105	12-13	0.260236261644998
1105	14-15	0.2863025072255496
1105	16-17	0.24105710501535782
1105	18-19	0.304328317110226
1105	20-21	0.28917291559007197
1105	22-23	0.29059380748491037
1105	24-25	0.3221795615963856
1105	26-27	0.31962389591183893
1105	28-29	0.273313476343489
1105	30-31	0.23421269775648312
1105	32-33	0.25200095980902404
1105	34-35	0.22358654140742118
1105	36-37	0.24725387440670232
1105	38-39	0.24509880173843612
1105	40-41	0.2739968230540626
1105	42-43	0.27801688736283126
1105	44-45	0.23548079716320558
1105	46-47	0.1600588427643146
1105	48-49	0.20176290097781902
1105	50-51	0.21122852822750104
1105	52-53	0.16815422219954712
1105	54-55	0.11089461323658867
1105	56-57	0.1330571069670441
1105	58-59	0.14043821196193562
1105	60-61	0.1202154270947915
1105	62-63	0.20177152887879402
1105	64-65	0.18101131458264064
1105	66-67	0.13277973408415278
1105	68-69	0.140450699165946
1105	70-71	0.15635481774442184
1105	72-73	0.16870075949027807
1105	74-75	0.14279886069801506
1105	76-77	0.11201151130077847
1105	78-79	0.09726860901515266
1105	80-81	0.10970035294939606
1105	82-83	0.0738665493892583
1105	84-85	0.07465115289656765
1105	86-87	0.12393800859803505
1105	88-89	0.09174495137223815
1105	90-91	0.09318201489022471
1105	92-93	0.01126113541997853
1105	94-95	0.028353778480258995
1105	96-97	0.08224666483598142
1105	98-99	0.08933087697947428
1105	100	0.10591780963929764
1106	1	0.1959969219520019
1106	2	0.224862613783106
1106	3	0.2453144221881196
1106	4	0.2718644082866746
1106	5	0.24913532886452572
1106	6	0.3055467002222798
1106	7	0.33893762849414344
1106	8	0.35179559032786045
1106	9	0.4035991948731734
1106	10-11	0.3371785882267986
1106	12-13	0.3908003643234892
1106	14-15	0.48214489930773397
1106	16-17	0.4255609789361614
1106	18-19	0.437111319277669
1106	20-21	0.44182667543169885
1106	22-23	0.42420907424848053
1106	24-25	0.455377580815707
1106	26-27	0.46579183239943944
1106	28-29	0.40626224207869654
1106	30-31	0.39508509877017417
1106	32-33	0.4051688723915703
1106	34-35	0.3681245859867843
1106	36-37	0.4542872408111194
1106	38-39	0.4157715620456628
1106	40-41	0.45927442730868506
1106	42-43	0.39963815001322445
1106	44-45	0.3492967587833462
1106	46-47	0.3641323659678264
1106	48-49	0.32234013559944685
1106	50-51	0.4033799483715015
1106	52-53	0.3775674431716638
1106	54-55	0.37165796741678037
1106	56-57	0.36824680719323055
1106	58-59	0.35624479187246294
1106	60-61	0.37432550128015407
1106	62-63	0.447156114821901
1106	64-65	0.3633082213406382
1106	66-67	0.3731946859991808
1106	68-69	0.41437612834217674
1106	70-71	0.3853961091206415
1106	72-73	0.39636401872422766
1106	74-75	0.4046996164906602
1106	76-77	0.38360289585904894
1106	78-79	0.3606646190230762
1106	80-81	0.359026659391219
1106	82-83	0.37159381341055564
1106	84-85	0.37210018723640204
1106	86-87	0.3812720979066633
1106	88-89	0.3784813146226007
1106	90-91	0.37616265323031683
1106	92-93	0.28719829902273375
1106	94-95	0.30700637665558617
1106	96-97	0.3908182070793984
1106	98-99	0.4519974561720481
1106	100	0.4290314389870957
1107	1	0.18455045718049945
1107	2	0.20240642156498723
1107	3	0.2218437386082961
1107	4	0.18271335843210323
1107	5	0.21691708541279553
1107	6	0.17644883120627242
1107	7	0.19531292707399217
1107	8	0.1741087722988226
1107	9	0.24545563364318923
1107	10-11	0.24514572777579247
1107	12-13	0.27743815782611847
1107	14-15	0.3237477539017064
1107	16-17	0.29575040561198307
1107	18-19	0.3234628146757643
1107	20-21	0.34486275379497755
1107	22-23	0.2815302503884851
1107	24-25	0.3311137679325924
1107	26-27	0.4108172490455928
1107	28-29	0.38308049351384454
1107	30-31	0.3476459548353432
1107	32-33	0.3336776447894394
1107	34-35	0.39192858355229276
1107	36-37	0.4205310792755057
1107	38-39	0.39530399854177745
1107	40-41	0.435734743283561
1107	42-43	0.4093281617815734
1107	44-45	0.3837196062868955
1107	46-47	0.36717582072075317
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2216	96-97	-0.8270498657853622
2216	98-99	-0.882169164214595
2216	100	-0.955378997158558
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	115865.0
3	22973.0
4	14210.0
5	11809.0
6	10533.0
7	10836.0
8	12258.0
9	13509.0
10	14804.0
11	16622.0
12	17618.0
13	19388.0
14	20815.0
15	22102.0
16	22386.0
17	23336.0
18	23124.0
19	21160.0
20	21612.0
21	23596.0
22	27228.0
23	32510.0
24	39170.0
25	48674.0
26	59102.0
27	68530.0
28	83189.0
29	106414.0
30	129028.0
31	152191.0
32	195718.0
33	261425.0
34	362380.0
35	536109.0
36	914108.0
37	1584750.0
38	1944587.0
39	653095.0
40	1216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.144453291921394	19.9148342130496	14.656218018559839	23.284494476469174
2	15.576180815504442	20.10990271066014	40.04150997903344	24.272406494801977
3	18.63804132894149	25.125238029313195	29.941279572448416	26.2954410692969
4	13.214333055814029	17.048265504350525	37.52881140114599	32.20859003868946
5	13.83552382331712	37.59403975334803	34.72629333677407	13.844143086560779
6	31.26215402769672	36.9180252253984	17.740143214946087	14.079677531958785
7	27.46588235171192	32.375639461013776	22.163697840042797	17.994780347231508
8	25.202731248134953	36.01179527261876	20.23409019230656	18.551383286939725
9	25.31201663670826	17.229490247455885	21.13385111483393	36.32464200100192
10-11	24.282388750086767	26.934673425899376	28.362970874888266	20.41996694912559
12-13	24.61874803524652	24.532491520950035	28.28351691493576	22.56524352886769
14-15	22.141980659836864	26.647097787109846	25.45584393094836	25.75507762210492
16-17	21.98226228771275	29.769431158522657	26.240068099608997	22.00823845415559
18-19	21.98888034008161	28.866576813862938	27.85824343536596	21.286299410689498
20-21	22.863094283085314	27.999232335271234	27.54428193277292	21.593391448870534
22-23	22.80397821976592	27.788422075782858	27.453804178364926	21.953795526086296
24-25	22.465637116113495	27.941404546289515	27.393608389645692	22.1993499479513
26-27	22.228035631081553	28.054677558874968	27.48801057879352	22.229276231249962
28-29	22.27791572187206	28.032238106199	27.63190965233191	22.05793651959704
30-31	22.427000966083263	28.142504953666204	27.340332621259122	22.090161458991414
32-33	22.290659613983944	28.096350299378127	27.4636971288771	22.149292957760828
34-35	22.51957534067322	27.851447853026727	27.444929295272065	22.184047511027984
36-37	22.52584560261046	27.914236819140985	27.295721074094686	22.264196504153865
38-39	22.306577271037984	28.10759552650931	27.409890111397118	22.175937091055587
40-41	22.434789433933876	27.815458606977167	27.49222509120083	22.257526867888128
42-43	22.32980109773853	28.01521904557682	27.374471082076518	22.280508774608126
44-45	22.35730820346609	28.00097512675766	27.352645135817355	22.289071533958893
46-47	22.43760549336398	27.9110846241016	27.306284846237226	22.345025036297194
48-49	22.317887571515826	27.924821032076924	27.296011374225102	22.46128002218214
50-51	22.258213185578114	27.962932514614735	27.305829347634873	22.47302495217227
52-53	22.38561297684821	27.951337602775737	27.237833225330494	22.425216195045554
54-55	22.337619630581443	28.11560595806723	27.181572587269827	22.365201824081502
56-57	22.338004125935733	27.90106896869239	27.2613334256303	22.499593479741577
58-59	22.343708090215777	27.680397993266485	27.392326378088377	22.583567538429357
60-61	22.348037299379108	27.87948306266325	27.24542051975159	22.52705911820605
62-63	22.191846212717827	27.968612254973024	27.289217049920268	22.55032448238888
64-65	22.344679262011553	27.859301637424437	27.191834651256926	22.604184449307084
66-67	22.168300649646916	27.8174085156397	27.26975969914087	22.744531135572515
68-69	22.213354401916128	27.882562371696583	27.277552392786713	22.626530833600576
70-71	22.258223551339075	27.690068938340158	27.311276730431754	22.740430779889014
72-73	22.148658142512463	27.78300721784631	27.360715601600173	22.707619038041052
74-75	22.11452272120831	27.8102856459212	27.269427019382515	22.805764613487973
76-77	22.211079564431635	27.71030549506648	27.119199760697676	22.95941517980421
78-79	22.16907643453305	27.713082485360545	27.315329252738085	22.802511827368317
80-81	22.08755743515933	27.716111623387306	27.313210021341455	22.883120920111903
82-83	22.032812345526487	27.706460850428567	27.453902981262907	22.806823822782043
84-85	22.047045370458523	27.54366230954488	27.35165145010806	23.057640869888534
86-87	22.157816484635656	27.653167386597506	27.30066161195723	22.888354516809603
88-89	22.077628816451497	27.56467440950957	27.333769588463745	23.02392718557519
90-91	22.03800298792488	27.495197418144535	27.343646232201653	23.12315336172894
92-93	22.032292345137293	27.574424044754164	27.28442957862587	23.10885403148267
94-95	21.960846875192434	27.358792370190432	27.45560848102862	23.22475227358851
96-97	22.105584846225074	27.42388960646313	27.306388819598354	23.164136727713444
98-99	21.97146070165573	27.457915269893256	27.319803986584706	23.25082004186631
100	22.05032317479438	27.284966071021405	27.367082008591353	23.297628745592863
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3117.0
1	2429.0
2	2102.5
3	2586.0
4	2582.0
5	2307.5
6	2100.5
7	1999.0
8	2027.0
9	2088.0
10	1904.0
11	1667.5
12	1662.0
13	1766.5
14	1857.0
15	2017.5
16	2290.5
17	2680.0
18	3367.5
19	4287.0
20	5720.5
21	7862.5
22	11263.0
23	17000.0
24	24737.0
25	34457.5
26	46375.0
27	60260.0
28	77433.5
29	97024.0
30	114414.5
31	130504.0
32	146197.0
33	160469.5
34	174708.0
35	188467.5
36	203433.5
37	219217.0
38	235026.0
39	246011.5
40	255264.5
41	263579.0
42	268173.5
43	271544.5
44	271732.0
45	273174.5
46	271742.5
47	264302.0
48	259987.0
49	257470.5
50	251838.5
51	243699.5
52	229855.0
53	218957.0
54	214327.0
55	206832.0
56	190653.0
57	169239.5
58	152961.5
59	141869.0
60	128327.0
61	111639.0
62	96961.0
63	81984.5
64	66913.5
65	55091.0
66	44744.0
67	35714.0
68	28565.0
69	22375.0
70	16983.0
71	12752.5
72	9860.5
73	7282.0
74	5044.0
75	3486.0
76	2274.0
77	1519.0
78	1018.5
79	634.0
80	430.5
81	283.0
82	201.0
83	156.0
84	122.5
85	100.5
86	83.0
87	55.0
88	35.5
89	36.5
90	37.5
91	26.5
92	21.0
93	17.0
94	14.5
95	15.5
96	11.0
97	11.5
98	13.0
99	16.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07940736329946017
2	0.013397788973071229
3	0.01775925243993847
4	0.011164824144226022
5	0.009310549257114801
6	0.007038409606710909
7	0.02000527554263657
8	0.008814334850704755
9	0.00908855860161557
10-11	0.013012569894410797
12-13	0.015239005586329554
14-15	0.009845938485083534
16-17	0.012549001172632992
18-19	0.008376882676632742
20-21	0.012947278525146318
22-23	0.01354795912237953
24-25	0.015780923951224733
26-27	0.0051710764457467895
28-29	0.01165450941370962
30-31	0.01008751655136211
32-33	0.012287835695575072
34-35	0.008383411813559189
36-37	0.010159337057553036
38-39	0.01898020104518424
40-41	0.012757933554279326
42-43	0.007848022585590456
44-45	0.02010321259653329
46-47	0.01823587943556917
48-49	0.017896364315393876
50-51	0.010590260094698602
52-53	0.010107103962141453
54-55	0.011308465156607878
56-57	0.02004445036419526
58-59	0.002115440364169141
60-61	0.00696658910051998
62-63	0.015278180407888241
64-65	0.02443203037876829
66-67	0.012725287869647087
68-69	0.014122523171906952
70-71	0.025274289042280077
72-73	0.020122800007312633
74-75	0.020148916555018423
76-77	0.00940848631101152
78-79	0.011040770542623512
80-81	0.019848576256401816
82-83	0.012052786766222947
84-85	0.0112431737873434
86-87	0.016009443743650414
88-89	0.014958252698492292
90-91	0.015160655943212179
92-93	0.007737027257840841
94-95	0.015624224664989984
96-97	0.013188856591424892
98-99	0.009780647115819054
100	0.006711952760388509
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7657980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.01610775221972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.36445408706142	33.306111016182385
2	15.637154378282045	15.0167057913137
3	5.977050741252862	8.609841372974463
4	2.900327412973955	5.570497343122962
5	1.5716370020480481	3.7731945818857318
6	0.9774036692381101	2.8158671939731206
7	0.685130557474502	2.302811187042384
8	0.48539160331203207	1.8645292421322575
9	0.35223887947726945	1.5221825992351534
>10	1.875806582106517	16.129109413235643
>50	0.1179860233250302	3.889983309493952
>100	0.05337547147747117	4.377954326848899
>500	0.0015804527431660111	0.48962883949805147
>1k	4.631392275807055E-4	0.3315837830612973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.3058273852895933E-5	0.0	0.0	0.0
4	0.0	1.3058273852895933E-5	0.0	0.0	0.0
5	0.0	2.6116547705791865E-5	0.0	0.0	0.0
6	0.0	2.6116547705791865E-5	0.0	0.0	0.0
7	0.0	2.6116547705791865E-5	0.0	0.0	0.0
8	0.0	2.6116547705791865E-5	0.0	0.0	0.0
9	1.0446619082316746E-4	2.6116547705791865E-5	0.0	0.0	0.0
10-11	1.1099532774961543E-4	2.6116547705791865E-5	0.0	0.0	0.0
12-13	1.175244646760634E-4	4.570395848513576E-5	0.0	0.0	0.0
14-15	1.175244646760634E-4	6.529136926447967E-5	0.0	1.3058273852895933E-5	0.0
16-17	1.175244646760634E-4	7.83496431173756E-5	0.0	2.6116547705791865E-5	0.0
18-19	1.175244646760634E-4	1.3058273852895933E-4	0.0	4.570395848513576E-5	0.0
20-21	1.175244646760634E-4	1.4364101238185527E-4	0.0	8.487878004382357E-5	0.0
22-23	1.175244646760634E-4	1.4364101238185527E-4	0.0	2.676946139843666E-4	0.0
24-25	1.175244646760634E-4	1.4364101238185527E-4	0.0	4.3745217407201376E-4	0.0
26-27	1.175244646760634E-4	1.4364101238185527E-4	0.0	8.879626219969234E-4	0.0
28-29	1.175244646760634E-4	1.4364101238185527E-4	0.0	0.0027030626875494583	0.0
30-31	1.175244646760634E-4	1.4364101238185527E-4	0.0	0.008866567946116339	0.0
32-33	1.175244646760634E-4	1.4364101238185527E-4	0.0	0.023015207665729082	0.0
34-35	1.175244646760634E-4	1.4364101238185527E-4	0.0	0.04633728476700122	0.0
36-37	1.175244646760634E-4	1.4364101238185527E-4	0.0	0.08309632566290327	0.0
38-39	1.175244646760634E-4	1.4364101238185527E-4	0.0	0.12904186221431763	0.0
40-41	1.175244646760634E-4	1.4364101238185527E-4	0.0	0.17473276242560049	0.0
42-43	1.175244646760634E-4	1.5017014930830322E-4	0.0	0.22332259943222627	0.0
44-45	1.3058273852895933E-4	1.6975756008764714E-4	0.0	0.2878108848547528	0.0
46-47	1.4364101238185527E-4	1.8281583394054308E-4	0.0	0.36936633420301435	0.0
48-49	1.4364101238185527E-4	1.8281583394054308E-4	0.0	0.4540949440975296	0.0
50-51	1.4364101238185527E-4	2.7422375091081463E-4	0.0	0.5375307848806082	0.0
52-53	1.6975756008764714E-4	3.1992770939595036E-4	0.0	0.6226576721276369	0.0
54-55	1.8281583394054308E-4	3.6563166788108615E-4	0.0	0.7217830289449698	0.0
56-57	1.95874107793439E-4	3.98277352513326E-4	0.0	0.8445308031621916	0.0
58-59	2.0893238164633492E-4	4.0480648943977393E-4	0.0	0.9825763451980809	0.0
60-61	2.0893238164633492E-4	4.0480648943977393E-4	0.0	1.1230441970336824	0.0
62-63	2.4810720320502276E-4	4.1786476329266984E-4	0.0	1.2682848479625175	0.0
64-65	2.4810720320502276E-4	4.439813109984617E-4	0.0	1.4220917265388522	0.0
66-67	2.4810720320502276E-4	4.439813109984617E-4	0.0	1.6054886536658493	0.0
68-69	2.6116547705791867E-4	4.439813109984617E-4	0.0	1.8153873475772984	0.0
70-71	2.6116547705791867E-4	4.439813109984617E-4	0.0	2.041197548178501	0.0
72-73	2.6116547705791867E-4	4.8315613255714955E-4	0.0	2.272524869482553	0.0
74-75	2.8728202476371054E-4	5.092726802629414E-4	0.0	2.5099634629497594	0.0
76-77	2.8728202476371054E-4	5.158018171893894E-4	0.0	2.7737275365044045	0.0
78-79	2.8728202476371054E-4	5.615057756745252E-4	0.0	3.0733692174698812	0.0
80-81	2.8728202476371054E-4	5.745640495274211E-4	0.0	3.3984875907223575	0.0
82-83	2.8728202476371054E-4	5.745640495274211E-4	0.0	3.73587421225963	0.0
84-85	3.0034029861660645E-4	5.81093186453869E-4	0.0	4.072842969033609	0.0
86-87	3.0034029861660645E-4	5.941514603067649E-4	0.0	4.436241672085851	0.0
88	3.0034029861660645E-4	6.006805972332129E-4	0.0	4.729550090232673	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8400	0.0	45.18336	1
GTATCAA	14015	0.0	35.26906	1
TCAACGC	14995	0.0	31.624416	4
ATCAACG	15060	0.0	31.519955	3
TATCAAC	16940	0.0	28.549015	2
CAACGCA	16830	0.0	28.177647	5
AACGCAG	17755	0.0	26.842182	6
GTACATG	25780	0.0	24.500637	1
ACGCAGA	20005	0.0	23.51807	7
TACATGG	26500	0.0	22.719168	2
CGCAGAG	20510	0.0	22.571024	8
ACATGGG	26755	0.0	21.747276	3
GTGGTAT	4575	0.0	20.046003	1
GCAGAGT	22745	0.0	19.63004	9
CATGGGG	19130	0.0	18.646812	4
CAGAGTA	21240	0.0	17.724163	10-11
GTATAGG	2775	0.0	17.626034	1
AGAGTAC	19930	0.0	16.731422	10-11
GAGTACA	19465	0.0	16.502531	1
TGGTATC	5520	0.0	15.58121	2
>>END_MODULE