FastQCFastQC Report
Wed 25 May 2016
SRR2049471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7657980
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280710.36655880532464175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268520.3506407694979616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155980.20368295555747076No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80220.10475347284793117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155200.057.6458781
GTATCAA279050.040.9491161
GTGGTAT61400.034.7222371
ATCAACG346550.032.661843
TCAACGC350300.032.325614
TATCAAC356600.031.8204272
CAACGCA367350.030.7996715
TGGTATC68250.030.1662642
AACGCAG380750.029.7484846
ACGCAGA428600.026.2408687
CGCAGAG435850.025.6318428
GCAGAGT466100.023.6759079
GTACATG258300.023.2672141
TACATGG263900.022.2470842
GAGTACT313650.021.97477512-13
CAGAGTA453000.021.37775210-11
ACATGGG266700.020.9383983
AGAGTAC436950.020.43664210-11
GTACTTT340300.020.08812714-15
AGTACTT323300.019.6398112-13
Produced by FastQC (version 0.11.5)